arecaidine propargyl ester tosylate  (Tocris)

Journal: Molecular Brain

Article Title: Involvement of muscarinic receptors in psychomotor hyperactivity in dopamine-deficient mice

doi: 10.1186/s13041-022-00984-x

Figure Lengend Snippet: Effects of CHRM subtype-selective agonists and antagonists on CHRM density and locomotor activity in DD mice. ( a – c ) Binding assays with [ 3 H]pirenzepine, [ 3 H]AFDX-384, and [ 3 H]4-DAMP were conducted. WT mice: n = 5, DD mice: n = 5. ** p < 0.01 (Student’s t -test). The data are expressed as the mean + SEM with data point overlap. ( d - h ) Change in locomotor activity in WT mice ( n = 11–12) and DD mice ( n = 9–12) following xanomeline, arecaidine propargyl ester tosylate, VU0255035, AQRA-741, and saline treatment. * p < 0.05, ** p < 0.01 (two-way repeated-measures ANOVA). The data are expressed as the mean ± SEM

Article Snippet: Xanomeline (CHRM1/CHRM4 agonist; 10 mg/kg; Tocris Bioscience, Bristol, UK), arecaidine propargyl ester tosylate (CHRM2 agonist; 5 mg/kg; Tocris Bioscience), VU0255035 (CHRM1 antagonist; 10 mg/kg; Tocris Bioscience), and AQRA-741 (CHRM2 antagonist; 1 mg/kg; Tocris Bioscience) were dissolved in saline and administered subcutaneously.

Techniques: Activity Assay, Binding Assay, Saline

Journal: Scientific Reports

Article Title: Molecular basis for epitope recognition by non-neutralizing anti-gp41 antibody F240

doi: 10.1038/srep36685

Figure Lengend Snippet: ( A ) Surface Plasmon Resonance (SPR) analysis of mAb F240 binding to the gp41 583–618 peptide (red lines) and the gp41 583–618 peptide pretreated in reducing (with 0.05 nM TCEP in PBS) conditions (green lines). Sensorgrams were obtained at room temperature for mAb F240 immobilized on a Protein A chip with 0–200 nM concentrations of gp41 peptide passed over the chip. Black and red/green curves correspond to the experimental data (for concentrations of peptide in a range of 3.1–200 nM) and best fit using the BIAevaluation software, respectively. Standard deviations of k a , k d and K D for two experiments are shown. (B ) Isothermal titration calorimetry (ITC) curves for mAb F240 binding to gp41 583–618 (left) and gp41 583–618 C698AC605A mutant (right), respectively. The binding isotherms were fitted to a one-site binding model using Origin for ITC version 7.0383 (MicroCal). The fitting yields the stoichiometry (N), the binding constant (K D ), and the enthalpy (ΔH) of the binding reaction.

Article Snippet: Data were corrected for the heat of dilution and fitted using a nonlinear least-squares routine using a single-site binding model with Origin for ITC version 7.0383 (MicroCal).

Techniques: SPR Assay, Binding Assay, Software, Isothermal Titration Calorimetry, Mutagenesis