other:
Amplification:Article Title: High-throughput evaluation of polymeric nanoparticles for tissue-targeted gene expression using barcoded plasmid DNA
Article Snippet: .. 1,4-butanediol diacrylate (B4) (Alfa Aesar, Ward Hill, MA), 1,5-pentanediol diacrylate (B5) (Monomer Polymer & Dajac Labs), 3-amino-1-propanol (S3) (Alfa Aesar), 4-amino-1-pentanol (S4) (Alfa Aesar), 5-amino-1-pentanol (S5) (Alfa Aesar), 2-methylpentane-1,5-diamine (E4) (TCI America, Portland, OR), 2-(3-aminopropylamino)ethanol (E6) (Sigma-Aldrich, St. Louis, MO), and 1-(3-aminopropyl)-4-methylpiperazine (E7) (Alfa Aesar) were purchased and used as received. pEGFP-N1 was purchased from Elim Biopharmaceuticals and amplified by Aldevron (Fargo, ND). pfLuc Luciferase-pcDNA3 was a gift from William Kaelin (Addgene plasmid # 18964) and amplified by Aldevron (Fargo, ND). .. Purelink Genomic DNA Extraction kit (Thermo Fisher Scientific), PowerUp SYBR Green Master Mix (Applied Biosystems, Foster City, CA), Label IT®-TrackerTM CyTM3 and CyTM5 kit (Mirus Bio LLC, Madison, WI) were obtained from commercial vendors and used per manufacturer’s instructions.
Article Title: Poly(β-amino ester)-co-polyethylene glycol (PEG-PBAE-PEG) polymers for gene and drug delivery
Article Snippet: .. Materials 1,4-butanediol diacrylate (B4), 4-amino-1-pentanol (S4), 5-amino-1-pentanol (S5), 1-(3-aminopropyl)-4-methylpiperazine (E7) (Alfa Aesar), 1,5-pentanediol diacrylate (B5) (Monomer Polymer & Dajac Labs), 2-methylpentane-1,5-diamine (E4) (TCI America), 2-(3-aminopropylamino)ethanol (E6) (Fluka), poly(ethylene glycol) methyl ether thiol (800 Da), branched 25 kDa poly(ethylenimine) (PEI) (Sigma-Aldrich), α-Mercaptoethyl-co-methoxy polyoxyethylene (5000 Da) (NOF America Corporation), and cell culture media components were purchased and used as received. pEGFP-N1 (EGFP) DNA (purchased from Elim Biopharmaceuticals and amplified by Aldevron, Fargo, ND), ganciclovir (Invivogen, San Diego, CA), Label IT-Tracker Cy3 kit (Mirus Bio LLC), and CellTiter 96 AQueous One MTS assay (Promega, Fitchburg, WI) were obtained from commercial vendors and used per manufacturer's instructions. .. HSV-tk gene was cloned into the pcDNA3.1 vector (Life Technologies) and amplified (Aldevron, Fargo, ND).
Luciferase:Article Title: High-throughput evaluation of polymeric nanoparticles for tissue-targeted gene expression using barcoded plasmid DNA
Article Snippet: .. 1,4-butanediol diacrylate (B4) (Alfa Aesar, Ward Hill, MA), 1,5-pentanediol diacrylate (B5) (Monomer Polymer & Dajac Labs), 3-amino-1-propanol (S3) (Alfa Aesar), 4-amino-1-pentanol (S4) (Alfa Aesar), 5-amino-1-pentanol (S5) (Alfa Aesar), 2-methylpentane-1,5-diamine (E4) (TCI America, Portland, OR), 2-(3-aminopropylamino)ethanol (E6) (Sigma-Aldrich, St. Louis, MO), and 1-(3-aminopropyl)-4-methylpiperazine (E7) (Alfa Aesar) were purchased and used as received. pEGFP-N1 was purchased from Elim Biopharmaceuticals and amplified by Aldevron (Fargo, ND). pfLuc Luciferase-pcDNA3 was a gift from William Kaelin (Addgene plasmid # 18964) and amplified by Aldevron (Fargo, ND). .. Purelink Genomic DNA Extraction kit (Thermo Fisher Scientific), PowerUp SYBR Green Master Mix (Applied Biosystems, Foster City, CA), Label IT®-TrackerTM CyTM3 and CyTM5 kit (Mirus Bio LLC, Madison, WI) were obtained from commercial vendors and used per manufacturer’s instructions.
Plasmid Preparation:Article Title: High-throughput evaluation of polymeric nanoparticles for tissue-targeted gene expression using barcoded plasmid DNA
Article Snippet: .. 1,4-butanediol diacrylate (B4) (Alfa Aesar, Ward Hill, MA), 1,5-pentanediol diacrylate (B5) (Monomer Polymer & Dajac Labs), 3-amino-1-propanol (S3) (Alfa Aesar), 4-amino-1-pentanol (S4) (Alfa Aesar), 5-amino-1-pentanol (S5) (Alfa Aesar), 2-methylpentane-1,5-diamine (E4) (TCI America, Portland, OR), 2-(3-aminopropylamino)ethanol (E6) (Sigma-Aldrich, St. Louis, MO), and 1-(3-aminopropyl)-4-methylpiperazine (E7) (Alfa Aesar) were purchased and used as received. pEGFP-N1 was purchased from Elim Biopharmaceuticals and amplified by Aldevron (Fargo, ND). pfLuc Luciferase-pcDNA3 was a gift from William Kaelin (Addgene plasmid # 18964) and amplified by Aldevron (Fargo, ND). .. Purelink Genomic DNA Extraction kit (Thermo Fisher Scientific), PowerUp SYBR Green Master Mix (Applied Biosystems, Foster City, CA), Label IT®-TrackerTM CyTM3 and CyTM5 kit (Mirus Bio LLC, Madison, WI) were obtained from commercial vendors and used per manufacturer’s instructions.
Cell Culture:Article Title: Poly(β-amino ester)-co-polyethylene glycol (PEG-PBAE-PEG) polymers for gene and drug delivery
Article Snippet: .. Materials 1,4-butanediol diacrylate (B4), 4-amino-1-pentanol (S4), 5-amino-1-pentanol (S5), 1-(3-aminopropyl)-4-methylpiperazine (E7) (Alfa Aesar), 1,5-pentanediol diacrylate (B5) (Monomer Polymer & Dajac Labs), 2-methylpentane-1,5-diamine (E4) (TCI America), 2-(3-aminopropylamino)ethanol (E6) (Fluka), poly(ethylene glycol) methyl ether thiol (800 Da), branched 25 kDa poly(ethylenimine) (PEI) (Sigma-Aldrich), α-Mercaptoethyl-co-methoxy polyoxyethylene (5000 Da) (NOF America Corporation), and cell culture media components were purchased and used as received. pEGFP-N1 (EGFP) DNA (purchased from Elim Biopharmaceuticals and amplified by Aldevron, Fargo, ND), ganciclovir (Invivogen, San Diego, CA), Label IT-Tracker Cy3 kit (Mirus Bio LLC), and CellTiter 96 AQueous One MTS assay (Promega, Fitchburg, WI) were obtained from commercial vendors and used per manufacturer's instructions. .. HSV-tk gene was cloned into the pcDNA3.1 vector (Life Technologies) and amplified (Aldevron, Fargo, ND).
MTS Assay:Article Title: Poly(β-amino ester)-co-polyethylene glycol (PEG-PBAE-PEG) polymers for gene and drug delivery
Article Snippet: .. Materials 1,4-butanediol diacrylate (B4), 4-amino-1-pentanol (S4), 5-amino-1-pentanol (S5), 1-(3-aminopropyl)-4-methylpiperazine (E7) (Alfa Aesar), 1,5-pentanediol diacrylate (B5) (Monomer Polymer & Dajac Labs), 2-methylpentane-1,5-diamine (E4) (TCI America), 2-(3-aminopropylamino)ethanol (E6) (Fluka), poly(ethylene glycol) methyl ether thiol (800 Da), branched 25 kDa poly(ethylenimine) (PEI) (Sigma-Aldrich), α-Mercaptoethyl-co-methoxy polyoxyethylene (5000 Da) (NOF America Corporation), and cell culture media components were purchased and used as received. pEGFP-N1 (EGFP) DNA (purchased from Elim Biopharmaceuticals and amplified by Aldevron, Fargo, ND), ganciclovir (Invivogen, San Diego, CA), Label IT-Tracker Cy3 kit (Mirus Bio LLC), and CellTiter 96 AQueous One MTS assay (Promega, Fitchburg, WI) were obtained from commercial vendors and used per manufacturer's instructions. .. HSV-tk gene was cloned into the pcDNA3.1 vector (Life Technologies) and amplified (Aldevron, Fargo, ND).
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