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male c57bl 6j mice  (Valiant Co Ltd)

 
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    Valiant Co Ltd male c57bl 6j mice
    Male C57bl 6j Mice, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/0296023710/Fat+free+diet/pmc04430618-40-2-21
    Average 94 stars, based on 9 article reviews
    male c57bl 6j mice - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Mouse Assay:

    Article Title: Alleviating oxidative stress disorders with PUFA derivatives
    Article Snippet: .. Mice were fed with either D-PUFA or H-PUFA (fat-free diet (MPBio) was supplemented with 10% fat (saturated and monounsaturated (oleic acid), of which 10% (i.e. 1% of the total fat) was a mixture of LA:ALA (1:1), or D2-LA:D4-ALA (1:1)) for six days, and then challenged with MPTP or saline. ..

    Article Title: Alleviating oxidative stress disorders with PUFA derivatives
    Article Snippet: .. Mice were fed with either D-PUFA or H-PUFA (fat-free diet (MPBio) was supplemented with 10% fat (saturated and monounsaturated (oleic acid), of which 10% (i.e. 1% of the total fat) was a mixture of LA:ALA (1:1), or D2-LA:D4-ALA (1:1)) for six days, and then challenged with MPTP or saline. ..

    Article Title: DGAT2 inhibition blocks SREBP-1 cleavage and improves hepatic steatosis by increasing phosphatidylethanolamine in the ER
    Article Snippet: 5×10 11 GC of the scAAV8.CB6-mDgat2 or AAV-DJ-eGFP (VectorBiolabs, Cat# 7118) was injected into 8-week-old C57Bl/6J mice. .. Mice were housed for 5 additional weeks and fed the chow diet after the AAV injection and were then switched to a fat-free diet (MP biomedicals, Cat# 960238) for one week before samples were collected for study. .. In the DGAT2, Insig-1 and Insig-2 knockout mice study, control scramble sgRNA or sgRNAs specific for mouse Dgat2 , Insig-1 and Insig-2 were designed through CRISPick program developed by the Broad Institute ( https://portals.broadinstitute.org/gppx/crispick/public ).

    Saline:

    Article Title: Alleviating oxidative stress disorders with PUFA derivatives
    Article Snippet: .. Mice were fed with either D-PUFA or H-PUFA (fat-free diet (MPBio) was supplemented with 10% fat (saturated and monounsaturated (oleic acid), of which 10% (i.e. 1% of the total fat) was a mixture of LA:ALA (1:1), or D2-LA:D4-ALA (1:1)) for six days, and then challenged with MPTP or saline. ..

    Article Title: Alleviating oxidative stress disorders with PUFA derivatives
    Article Snippet: .. Mice were fed with either D-PUFA or H-PUFA (fat-free diet (MPBio) was supplemented with 10% fat (saturated and monounsaturated (oleic acid), of which 10% (i.e. 1% of the total fat) was a mixture of LA:ALA (1:1), or D2-LA:D4-ALA (1:1)) for six days, and then challenged with MPTP or saline. ..

    other:

    Article Title: Insulin induction of SREBP-1c in rodent liver requires LXRα-C/EBPβ complex.
    Article Snippet: For rats, the fasted group was fasted for 48 h, and the refed group was fasted for 48 h and then refed for 6 h with a 61.2% carbohydrate, fat-free diet (MP Biomedicals; catalog no. 960238).

    Chromatin Immunoprecipitation:

    Article Title: BHLHE40, a third transcription factor required for insulin induction of SREBP-1c mRNA in rodent liver
    Article Snippet: .. Description Source or reference Identifier 3,30,5-Triiodo-L-thyronine Sigma-Aldrich, St. Louis, MO T2877 Bovine insulin Sigma-Aldrich I6634 Bio-Gen PRO 200 Homogenizer PRO Scientific, Oxford, CT 01–01200 Calpain inhibitor I (ALLN) AG Scientific, San Diego, CA CAS110044-82-1 Chromatin Immunoprecipitation (ChIP) Assay Kit EMD Millipore Corp, Billerica, MA 17–295 Collagen I-coated dishes BD Sciences, Franklin Lakes, NJ 356400 Dexamethasone Sigma-Aldrich D4902 DMEM Sigma-Aldrich D6046 Freund’s Adjuvant, Incomplete Sigma-Aldrich F5506 Halt Phosphatase Inhibitor Cocktail Thermo Fisher Scientific, Waltham, MA 78426 High-Carbohydrate/Fat-free Diet MP Biomedicals, Santa Ana, CA 960238 Lentiviurs Encoding Rat BHLHE40 Origene, Rockville, MD BR206868LV Lipofectamine 2000 Invitrogen, Carlsbad, CA 11668–027 Medium 199 Invitrogen 11150–059 nProtein A Sepharose 4 Fast Flow Affinity Media GE Healthcare, Chicago, IL 17-5280-04 PBS Sigma-Aldrich D8537 Phenylmethysulfonyl fluoride (PMSF) Sigma-Aldrich P7626 Pierce Co-Immuno- precipitation Kit Thermo Fisher Scientific 26149 Protease Inhibitor cOmplete Tablets Roche Holding AG, Basel, CH 5892791001 Proteinase K Thermo Fisher Scientific AM2546 ProteoSilver Stain Kit Sigma-Aldrich PROTSIL1 Rapamycin Sigma-Aldrich R0395 Teklad Global 18% Protein Rodent Diet (chow diet for mice) Harlan Laboratories, Indianapolis, IN 2018 Teklad Global 16% Protein Rodent Diet (chow diet for rats) Harlan Laboratories 2016 Tian et al. eLife 2018;7:e36826. ..

    Adjuvant:

    Article Title: BHLHE40, a third transcription factor required for insulin induction of SREBP-1c mRNA in rodent liver
    Article Snippet: .. Description Source or reference Identifier 3,30,5-Triiodo-L-thyronine Sigma-Aldrich, St. Louis, MO T2877 Bovine insulin Sigma-Aldrich I6634 Bio-Gen PRO 200 Homogenizer PRO Scientific, Oxford, CT 01–01200 Calpain inhibitor I (ALLN) AG Scientific, San Diego, CA CAS110044-82-1 Chromatin Immunoprecipitation (ChIP) Assay Kit EMD Millipore Corp, Billerica, MA 17–295 Collagen I-coated dishes BD Sciences, Franklin Lakes, NJ 356400 Dexamethasone Sigma-Aldrich D4902 DMEM Sigma-Aldrich D6046 Freund’s Adjuvant, Incomplete Sigma-Aldrich F5506 Halt Phosphatase Inhibitor Cocktail Thermo Fisher Scientific, Waltham, MA 78426 High-Carbohydrate/Fat-free Diet MP Biomedicals, Santa Ana, CA 960238 Lentiviurs Encoding Rat BHLHE40 Origene, Rockville, MD BR206868LV Lipofectamine 2000 Invitrogen, Carlsbad, CA 11668–027 Medium 199 Invitrogen 11150–059 nProtein A Sepharose 4 Fast Flow Affinity Media GE Healthcare, Chicago, IL 17-5280-04 PBS Sigma-Aldrich D8537 Phenylmethysulfonyl fluoride (PMSF) Sigma-Aldrich P7626 Pierce Co-Immuno- precipitation Kit Thermo Fisher Scientific 26149 Protease Inhibitor cOmplete Tablets Roche Holding AG, Basel, CH 5892791001 Proteinase K Thermo Fisher Scientific AM2546 ProteoSilver Stain Kit Sigma-Aldrich PROTSIL1 Rapamycin Sigma-Aldrich R0395 Teklad Global 18% Protein Rodent Diet (chow diet for mice) Harlan Laboratories, Indianapolis, IN 2018 Teklad Global 16% Protein Rodent Diet (chow diet for rats) Harlan Laboratories 2016 Tian et al. eLife 2018;7:e36826. ..

    Protease Inhibitor:

    Article Title: BHLHE40, a third transcription factor required for insulin induction of SREBP-1c mRNA in rodent liver
    Article Snippet: .. Description Source or reference Identifier 3,30,5-Triiodo-L-thyronine Sigma-Aldrich, St. Louis, MO T2877 Bovine insulin Sigma-Aldrich I6634 Bio-Gen PRO 200 Homogenizer PRO Scientific, Oxford, CT 01–01200 Calpain inhibitor I (ALLN) AG Scientific, San Diego, CA CAS110044-82-1 Chromatin Immunoprecipitation (ChIP) Assay Kit EMD Millipore Corp, Billerica, MA 17–295 Collagen I-coated dishes BD Sciences, Franklin Lakes, NJ 356400 Dexamethasone Sigma-Aldrich D4902 DMEM Sigma-Aldrich D6046 Freund’s Adjuvant, Incomplete Sigma-Aldrich F5506 Halt Phosphatase Inhibitor Cocktail Thermo Fisher Scientific, Waltham, MA 78426 High-Carbohydrate/Fat-free Diet MP Biomedicals, Santa Ana, CA 960238 Lentiviurs Encoding Rat BHLHE40 Origene, Rockville, MD BR206868LV Lipofectamine 2000 Invitrogen, Carlsbad, CA 11668–027 Medium 199 Invitrogen 11150–059 nProtein A Sepharose 4 Fast Flow Affinity Media GE Healthcare, Chicago, IL 17-5280-04 PBS Sigma-Aldrich D8537 Phenylmethysulfonyl fluoride (PMSF) Sigma-Aldrich P7626 Pierce Co-Immuno- precipitation Kit Thermo Fisher Scientific 26149 Protease Inhibitor cOmplete Tablets Roche Holding AG, Basel, CH 5892791001 Proteinase K Thermo Fisher Scientific AM2546 ProteoSilver Stain Kit Sigma-Aldrich PROTSIL1 Rapamycin Sigma-Aldrich R0395 Teklad Global 18% Protein Rodent Diet (chow diet for mice) Harlan Laboratories, Indianapolis, IN 2018 Teklad Global 16% Protein Rodent Diet (chow diet for rats) Harlan Laboratories 2016 Tian et al. eLife 2018;7:e36826. ..

    Staining:

    Article Title: BHLHE40, a third transcription factor required for insulin induction of SREBP-1c mRNA in rodent liver
    Article Snippet: .. Description Source or reference Identifier 3,30,5-Triiodo-L-thyronine Sigma-Aldrich, St. Louis, MO T2877 Bovine insulin Sigma-Aldrich I6634 Bio-Gen PRO 200 Homogenizer PRO Scientific, Oxford, CT 01–01200 Calpain inhibitor I (ALLN) AG Scientific, San Diego, CA CAS110044-82-1 Chromatin Immunoprecipitation (ChIP) Assay Kit EMD Millipore Corp, Billerica, MA 17–295 Collagen I-coated dishes BD Sciences, Franklin Lakes, NJ 356400 Dexamethasone Sigma-Aldrich D4902 DMEM Sigma-Aldrich D6046 Freund’s Adjuvant, Incomplete Sigma-Aldrich F5506 Halt Phosphatase Inhibitor Cocktail Thermo Fisher Scientific, Waltham, MA 78426 High-Carbohydrate/Fat-free Diet MP Biomedicals, Santa Ana, CA 960238 Lentiviurs Encoding Rat BHLHE40 Origene, Rockville, MD BR206868LV Lipofectamine 2000 Invitrogen, Carlsbad, CA 11668–027 Medium 199 Invitrogen 11150–059 nProtein A Sepharose 4 Fast Flow Affinity Media GE Healthcare, Chicago, IL 17-5280-04 PBS Sigma-Aldrich D8537 Phenylmethysulfonyl fluoride (PMSF) Sigma-Aldrich P7626 Pierce Co-Immuno- precipitation Kit Thermo Fisher Scientific 26149 Protease Inhibitor cOmplete Tablets Roche Holding AG, Basel, CH 5892791001 Proteinase K Thermo Fisher Scientific AM2546 ProteoSilver Stain Kit Sigma-Aldrich PROTSIL1 Rapamycin Sigma-Aldrich R0395 Teklad Global 18% Protein Rodent Diet (chow diet for mice) Harlan Laboratories, Indianapolis, IN 2018 Teklad Global 16% Protein Rodent Diet (chow diet for rats) Harlan Laboratories 2016 Tian et al. eLife 2018;7:e36826. ..

    Bioprocessing:

    Article Title: DGAT2 inhibition blocks SREBP-1 cleavage and improves hepatic steatosis by increasing phosphatidylethanolamine in the ER
    Article Snippet: 5×10 11 GC of the scAAV8.CB6-mDgat2 or AAV-DJ-eGFP (VectorBiolabs, Cat# 7118) was injected into 8-week-old C57Bl/6J mice. .. Mice were housed for 5 additional weeks and fed the chow diet after the AAV injection and were then switched to a fat-free diet (MP biomedicals, Cat# 960238) for one week before samples were collected for study. .. In the DGAT2, Insig-1 and Insig-2 knockout mice study, control scramble sgRNA or sgRNAs specific for mouse Dgat2 , Insig-1 and Insig-2 were designed through CRISPick program developed by the Broad Institute ( https://portals.broadinstitute.org/gppx/crispick/public ).

    Injection:

    Article Title: DGAT2 inhibition blocks SREBP-1 cleavage and improves hepatic steatosis by increasing phosphatidylethanolamine in the ER
    Article Snippet: 5×10 11 GC of the scAAV8.CB6-mDgat2 or AAV-DJ-eGFP (VectorBiolabs, Cat# 7118) was injected into 8-week-old C57Bl/6J mice. .. Mice were housed for 5 additional weeks and fed the chow diet after the AAV injection and were then switched to a fat-free diet (MP biomedicals, Cat# 960238) for one week before samples were collected for study. .. In the DGAT2, Insig-1 and Insig-2 knockout mice study, control scramble sgRNA or sgRNAs specific for mouse Dgat2 , Insig-1 and Insig-2 were designed through CRISPick program developed by the Broad Institute ( https://portals.broadinstitute.org/gppx/crispick/public ).

    Control:

    Article Title: Maternal omega-3 polyunsaturated fatty acids improved levels of DHA-enriched phosphatidylethanolamines and enriched lipid clustering in the neuronal membranes of C57BL/6 mice fetal brains during gestation.
    Article Snippet: .. Briefly, a fatfree semi-synthetic diet was procured from MP Biomedicals (USA) to control the total fat content of the diets at 20%. ..



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    Image Search Results


    DGAT2 and/or Insigs were knocked out using sgRNAs packaged in AAV-DJ and injected into Cas9-expressing mice. (A and B) Relative mRNA levels of Dgat2, Insig-1, Insig-2a, Insig-2b, and lipogenesis genes in Dgat2 and/or Insig-1 and −2 (Insig) hepatocyte-specific knockout mice (n = 5 per group). Total RNA was extracted from livers of the mice 6 weeks after the AAV injection and subjected to quantitative real-time PCR analysis. (C) Immunoblot analysis of precursor and nuclear forms of SREBP-1 in Dgat2 and/or Insig knockout mice. Membrane and nuclear proteins from individual livers were prepared and loaded to 8% SDS-PAGE (3 samples from each group were loaded for the blot on the left panel, and the other 2 samples from each group were loaded for the blot on the right panel) and subjected to immunoblot analysis. Precursor SREBP-1 (P) and Insig proteins were detected in membrane fractions, and nSREBP-1 (N) was measured in the nuclear protein fractions. Calnexin and LSD1 were used as loading controls for membrane and nuclear proteins, respectively. (D) Protein intensities of immunoblots from (C) were quantified, and intensities of the precursor (P) and nuclear (N) SREBP-1 were normalized to calnexin and LSD1, respectively. # denotes a nonspecific bond detected by Insig antibody. (E) PE and PC content in the ER fractions were measured using LC-MS/MS. Data are presented as mean ± SD. Statistical significance was assessed by two-tailed Student’s t test. *p < 0.05, **p < 0.01, ***p < 0.001.

    Journal: Cell metabolism

    Article Title: DGAT2 inhibition blocks SREBP-1 cleavage and improves hepatic steatosis by increasing phosphatidylethanolamine in the ER

    doi: 10.1016/j.cmet.2024.01.011

    Figure Lengend Snippet: DGAT2 and/or Insigs were knocked out using sgRNAs packaged in AAV-DJ and injected into Cas9-expressing mice. (A and B) Relative mRNA levels of Dgat2, Insig-1, Insig-2a, Insig-2b, and lipogenesis genes in Dgat2 and/or Insig-1 and −2 (Insig) hepatocyte-specific knockout mice (n = 5 per group). Total RNA was extracted from livers of the mice 6 weeks after the AAV injection and subjected to quantitative real-time PCR analysis. (C) Immunoblot analysis of precursor and nuclear forms of SREBP-1 in Dgat2 and/or Insig knockout mice. Membrane and nuclear proteins from individual livers were prepared and loaded to 8% SDS-PAGE (3 samples from each group were loaded for the blot on the left panel, and the other 2 samples from each group were loaded for the blot on the right panel) and subjected to immunoblot analysis. Precursor SREBP-1 (P) and Insig proteins were detected in membrane fractions, and nSREBP-1 (N) was measured in the nuclear protein fractions. Calnexin and LSD1 were used as loading controls for membrane and nuclear proteins, respectively. (D) Protein intensities of immunoblots from (C) were quantified, and intensities of the precursor (P) and nuclear (N) SREBP-1 were normalized to calnexin and LSD1, respectively. # denotes a nonspecific bond detected by Insig antibody. (E) PE and PC content in the ER fractions were measured using LC-MS/MS. Data are presented as mean ± SD. Statistical significance was assessed by two-tailed Student’s t test. *p < 0.05, **p < 0.01, ***p < 0.001.

    Article Snippet: Mice were housed for 5 additional weeks and fed the chow diet after the AAV injection and were then switched to a fat-free diet (MP biomedicals, Cat# 960238) for one week before samples were collected for study.

    Techniques: Injection, Expressing, Knock-Out, Real-time Polymerase Chain Reaction, Western Blot, Membrane, SDS Page, Liquid Chromatography with Mass Spectroscopy, Two Tailed Test