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Valiant Co Ltd hundred microlitre extraction buffer
Hundred Microlitre Extraction Buffer, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0230001106/Alcohol/pmc03806258-134-1-30
Average 96 stars, based on 113 article reviews
hundred microlitre extraction buffer - by Bioz Stars, 2026-09
96/100 stars

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Related Articles

Extraction:

Article Title: Dissolved oxygen concentrations influence microbial diversity, abundance and dominant players in an oxygen minimum zone
Article Snippet: .. Briefly, DNA was extracted using a CTAB extraction buffer ((10% CTAB (hexadecyltrimethylammonium bromide), 1M NaCl, and 0.5 M phosphate buffer, pH 8) with 0.1M ammonium aluminum sulfate, and 25:24:1 phenol:chloroform:isoamyl alcohol) paired with bead beating via FastPrep-24 (MP Biomedicals). .. Genomic DNA was purified with a QIAGEN AllPrep DNA/RNA Kit (Valencia, CA, USA).

Article Title: Impact of microbiological molecular methodologies on adaptive sampling using nanopore sequencing in metagenomic studies.
Article Snippet: .. Procurement information, cost (RRP excluding VAT), type of extraction method, and whether they employ a bead-beating technique are shown Commercial kit Abbreviation Provider Product no Cost (RRP excludingVAT) (£) Bead-beating Extraction methodology AllPrep DNA/RNA Mini Kit AP Qiagen 98,204 594.00 N Column-based RNeasy PowerSoil DNA Elution Kit PS Qiagen 12,866–25 497.00 (Including RNeasy PowerSoil Total RNA kit) Y Alcohol precipitation QIAamp PowerFecal Pro DNA Kit PF Qiagen 51,804 352.00 Y Column-based FastDNATM SPIN Kit BM MP Biomedicals 116,540,600-CF 405.00 Y Column-based Quick DNA/RNATM MagBead MB Zymo Research R2130 442.00 Y Magnetic bead sample using the ligation sequencing kit by ONT (either SQK-LSK109 or SQK-LSK112) and native barcoding kits (either EXP-NBD104 and EXP-NBD114 or SQKNBD112.24, respectively). ..

Article Title: Uncovering novel functions of the enigmatic, abundant, and active Anaerolineae in a salt marsh ecosystem
Article Snippet: .. Specifically, a modified CTAB extraction buffer ([10% CTAB {hexadecyltrimethylammonium bromide}, 1 M NaCl, and 0.5 M phosphate buffer, pH 8] with 0.1 M ammonium aluminum sulfate, 25:24:1 phenol:chloroform:isoamyl alcohol) and bead beating using a FastPrep-24 (MP Biomedicals) were used to extract DNA and RNA ( ). ..

Article Title: Reduced legacy precipitation decreases microbial community growth efficiency and alters soil organic carbon in a California grassland
Article Snippet: .. For each extraction, soil (0.4 g) was added to a 2 mL Lysing Matrix E tube (MP Biomedicals, Irvine, CA) and extracted twice as follows: each tube received 500 μL extraction buffer (5% cetyltrimethylammonium bromide [CTAB], 0.5 M NaCl, 240 mM K 2 HPO 4 , pH 8.0) and 500 μL of 25:24:1 phenol:chloroform:isoamyl alcohol, shaken (30 s, 5.5 ms -1 ) (FastPrep24, MP Biomedicals, Santa Clara, CA), and then centrifuged for 5 min (16,100 × g). .. To remove residual phenol, the supernatant was added to a pre-spun 2 mL phase lock gel tube (5 prime, Gaithersburg, MD) with an equal volume of 24:1 chloroform:isoamyl alcohol, mixed by inverting, and centrifuged for 2 min (16,100 × g).

Incubation:

Article Title: Functional variants of CFAP410 affect the DNA damage response leading to motor neuron degeneration – Implications for ALS
Article Snippet: For proteinase K based DNA isolation, media was aspirated and cells were washed with PBS before adding 150μl/cm2 of lysis buffer (0.2 mg/ml proteinase K (Roche), 100mM NaCl, 0.5M EDTA, 0.5% SDS in 10mM Tris pH 8.0, all Sigma). .. After incubation overnight at 50°C lysates were transferred to microfuge tubes with equal volumes of phenol:chloroform:isoamyl alcohol (PCI, 25:24:1, MP biomedical, Sigma, Sigma). ..

Article Title: A multiplexed, target-based phenotypic screening platform using CRISPR interference in Mycobacterium abscessus
Article Snippet: Briefly, 1.2-2.0 mL of each culture was spun down and resuspended in 0.5-0.7 mL TRIzol (Invitrogen) and stored at –80°C until ready for processing. .. Suspensions were transferred to FastPrep tubes (MP Biomedicals) containing ∼ 0.4-0.5 mL 0.1 mm zirconia/silica beads (BioSpec) and homogenized with a FastPrep bead beater (MP Biomedicals) at 10 m/s for 90 s. Samples were then incubated on ice for at least 3 minutes prior to addition of 200 μL 24:1 chloroform/isoamyl alcohol. ..

Lysis:

Article Title: A rapid ionic liquid-based DNA extraction method for molecular diagnostics of urinary tract infections
Article Snippet: .. In short, the pellet was resuspended in 0.9% NaCl and cell lysis was achieved by adding CTAB buffer, phenol, chloroform/isoamyl alcohol, and glass beads in a FastPrep 24 bench-top homogeniser (MP Biomedicals Inc., USA) at a speed setting of 6 m/s for 30 s. DNA was precipitated with isopropanol and washed with ethanol. ..

Ligation:

Article Title: Impact of microbiological molecular methodologies on adaptive sampling using nanopore sequencing in metagenomic studies.
Article Snippet: .. Procurement information, cost (RRP excluding VAT), type of extraction method, and whether they employ a bead-beating technique are shown Commercial kit Abbreviation Provider Product no Cost (RRP excludingVAT) (£) Bead-beating Extraction methodology AllPrep DNA/RNA Mini Kit AP Qiagen 98,204 594.00 N Column-based RNeasy PowerSoil DNA Elution Kit PS Qiagen 12,866–25 497.00 (Including RNeasy PowerSoil Total RNA kit) Y Alcohol precipitation QIAamp PowerFecal Pro DNA Kit PF Qiagen 51,804 352.00 Y Column-based FastDNATM SPIN Kit BM MP Biomedicals 116,540,600-CF 405.00 Y Column-based Quick DNA/RNATM MagBead MB Zymo Research R2130 442.00 Y Magnetic bead sample using the ligation sequencing kit by ONT (either SQK-LSK109 or SQK-LSK112) and native barcoding kits (either EXP-NBD104 and EXP-NBD114 or SQKNBD112.24, respectively). ..

Sequencing:

Article Title: Impact of microbiological molecular methodologies on adaptive sampling using nanopore sequencing in metagenomic studies.
Article Snippet: .. Procurement information, cost (RRP excluding VAT), type of extraction method, and whether they employ a bead-beating technique are shown Commercial kit Abbreviation Provider Product no Cost (RRP excludingVAT) (£) Bead-beating Extraction methodology AllPrep DNA/RNA Mini Kit AP Qiagen 98,204 594.00 N Column-based RNeasy PowerSoil DNA Elution Kit PS Qiagen 12,866–25 497.00 (Including RNeasy PowerSoil Total RNA kit) Y Alcohol precipitation QIAamp PowerFecal Pro DNA Kit PF Qiagen 51,804 352.00 Y Column-based FastDNATM SPIN Kit BM MP Biomedicals 116,540,600-CF 405.00 Y Column-based Quick DNA/RNATM MagBead MB Zymo Research R2130 442.00 Y Magnetic bead sample using the ligation sequencing kit by ONT (either SQK-LSK109 or SQK-LSK112) and native barcoding kits (either EXP-NBD104 and EXP-NBD114 or SQKNBD112.24, respectively). ..

Modification:

Article Title: Uncovering novel functions of the enigmatic, abundant, and active Anaerolineae in a salt marsh ecosystem
Article Snippet: .. Specifically, a modified CTAB extraction buffer ([10% CTAB {hexadecyltrimethylammonium bromide}, 1 M NaCl, and 0.5 M phosphate buffer, pH 8] with 0.1 M ammonium aluminum sulfate, 25:24:1 phenol:chloroform:isoamyl alcohol) and bead beating using a FastPrep-24 (MP Biomedicals) were used to extract DNA and RNA ( ). ..

Homogenization:

Article Title: Particle-associated and free-living Legionella spp. in drinking water.
Article Snippet: The Legionella genus houses several opportunistic pathogens that cause legionellosis, which ranges from mild (Pontiac Fever) to severe (Legionnaires’ disease).. Legionella pneumophila (Lp), the leading observed cause of legionellosis, persists in drinking water distribution systems (DWDSs) and building plumbing systems due to factors such as intrinsic disinfectant resistance and shielding by biofilms and free-living amoebae.. Remediation efforts, therefore, might be hindered if Lp is primarily associated with biofilms, free-living amoebae, or other particles.



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