Clinical Proteomics:
Article Title: Metabolic Signature of Arsenic Exposure and Metabolism: The Folic Acid and Creatine Trial
Article Snippet: .. 67 As previously described,8 plasma vitamin B12 was measured using a radio protein-binding assay 68 (SimulTRAC-SNB; MP Biomedicals), with intra- and inter-assay coefficients of variation (CVs) 69 of 6% and 17%, whereas plasma total homocysteine and cysteine concentrations were measured 70 by HPLC with fluorescence detection using the method of Pfeiffer et al,9 with intra- and inter71 assay CVs of 4% and 7% and of 5% and 8%, respectively. .. Plasma dimethylglycine 72 concentrations were measured using HPLC coupled with tandem mass spectrometry (MS/MS) at 73 Division of Nutritional Sciences Cornell University, with overall intra- and inter-assay CVs less 74 than 10%.10, 11 The analyses of 5-methyl-tetrahydrofolate (5-mTHF) and other OCM 75 intermediates, including methionine, SAM, S-adenosylhomocysteine (SAH), betaine, choline, 76 and cystathionine, were carried out at the Center of Metabolomics, Baylor Scott & White S3 77 Research Institute using HPLC-MS/MS-based methods,12, 13 with overall intra- and inter-assay 78 CVs of less than 15%.
Article Title: The Correlation Between Plasma Vitamin D and Blood Parameters in Prenatal Women
Article Snippet: .. Ferritin and total iron-binding capacity were analyzed by the turbidimetric method; red blood cell (RBC) counts were analyzed using a hematology analyzer; hemoglobin values were analyzed by the sodium lauryl sulfate hemoglobin detection method; iron concentration was tested using UniCel DxC 800 (Beckman Coulter, Brea, CA, USA); plasma folate and vitamin B12 were analyzed using SimulTRAC-SNB radioimmunoassay (MP Biomedicals, Irvine, CA, USA); and plasma vitamin D was analyzed using a Elecsys ® Vitamin D total reagent kit (Roche, Basel, Switzerland). .. Urinary iodine was tested using a spectrophotometer (405 nm) to determine absorbance (HITACHI Spectrometer F-7000, Tokyo, Japan).
Protein Binding:
Article Title: Metabolic Signature of Arsenic Exposure and Metabolism: The Folic Acid and Creatine Trial
Article Snippet: .. 67 As previously described,8 plasma vitamin B12 was measured using a radio protein-binding assay 68 (SimulTRAC-SNB; MP Biomedicals), with intra- and inter-assay coefficients of variation (CVs) 69 of 6% and 17%, whereas plasma total homocysteine and cysteine concentrations were measured 70 by HPLC with fluorescence detection using the method of Pfeiffer et al,9 with intra- and inter71 assay CVs of 4% and 7% and of 5% and 8%, respectively. .. Plasma dimethylglycine 72 concentrations were measured using HPLC coupled with tandem mass spectrometry (MS/MS) at 73 Division of Nutritional Sciences Cornell University, with overall intra- and inter-assay CVs less 74 than 10%.10, 11 The analyses of 5-methyl-tetrahydrofolate (5-mTHF) and other OCM 75 intermediates, including methionine, SAM, S-adenosylhomocysteine (SAH), betaine, choline, 76 and cystathionine, were carried out at the Center of Metabolomics, Baylor Scott & White S3 77 Research Institute using HPLC-MS/MS-based methods,12, 13 with overall intra- and inter-assay 78 CVs of less than 15%.
Concentration Assay:Article Title: Effect of supplying a portion of trace mineral amino acid complexes on serum folate concentration from the dry period to early lactation
Article Snippet: .. Serum folate concentration was analyzed in triplicate according to the manufacturer instructions (Folate/Vitamin B12 ChLIA Kit, MP Biomedicals, Solon, OH). ..
Article Title: The Correlation Between Plasma Vitamin D and Blood Parameters in Prenatal Women
Article Snippet: .. Ferritin and total iron-binding capacity were analyzed by the turbidimetric method; red blood cell (RBC) counts were analyzed using a hematology analyzer; hemoglobin values were analyzed by the sodium lauryl sulfate hemoglobin detection method; iron concentration was tested using UniCel DxC 800 (Beckman Coulter, Brea, CA, USA); plasma folate and vitamin B12 were analyzed using SimulTRAC-SNB radioimmunoassay (MP Biomedicals, Irvine, CA, USA); and plasma vitamin D was analyzed using a Elecsys ® Vitamin D total reagent kit (Roche, Basel, Switzerland). .. Urinary iodine was tested using a spectrophotometer (405 nm) to determine absorbance (HITACHI Spectrometer F-7000, Tokyo, Japan).
Inter Assay:Article Title: Metabolic Signature of Arsenic Exposure and Metabolism: The Folic Acid and Creatine Trial
Article Snippet: .. 67 As previously described,8 plasma vitamin B12 was measured using a radio protein-binding assay 68 (SimulTRAC-SNB; MP Biomedicals), with intra- and inter-assay coefficients of variation (CVs) 69 of 6% and 17%, whereas plasma total homocysteine and cysteine concentrations were measured 70 by HPLC with fluorescence detection using the method of Pfeiffer et al,9 with intra- and inter71 assay CVs of 4% and 7% and of 5% and 8%, respectively. .. Plasma dimethylglycine 72 concentrations were measured using HPLC coupled with tandem mass spectrometry (MS/MS) at 73 Division of Nutritional Sciences Cornell University, with overall intra- and inter-assay CVs less 74 than 10%.10, 11 The analyses of 5-methyl-tetrahydrofolate (5-mTHF) and other OCM 75 intermediates, including methionine, SAM, S-adenosylhomocysteine (SAH), betaine, choline, 76 and cystathionine, were carried out at the Center of Metabolomics, Baylor Scott & White S3 77 Research Institute using HPLC-MS/MS-based methods,12, 13 with overall intra- and inter-assay 78 CVs of less than 15%.
High Performance Liquid Chromatography:Article Title: Metabolic Signature of Arsenic Exposure and Metabolism: The Folic Acid and Creatine Trial
Article Snippet: .. 67 As previously described,8 plasma vitamin B12 was measured using a radio protein-binding assay 68 (SimulTRAC-SNB; MP Biomedicals), with intra- and inter-assay coefficients of variation (CVs) 69 of 6% and 17%, whereas plasma total homocysteine and cysteine concentrations were measured 70 by HPLC with fluorescence detection using the method of Pfeiffer et al,9 with intra- and inter71 assay CVs of 4% and 7% and of 5% and 8%, respectively. .. Plasma dimethylglycine 72 concentrations were measured using HPLC coupled with tandem mass spectrometry (MS/MS) at 73 Division of Nutritional Sciences Cornell University, with overall intra- and inter-assay CVs less 74 than 10%.10, 11 The analyses of 5-methyl-tetrahydrofolate (5-mTHF) and other OCM 75 intermediates, including methionine, SAM, S-adenosylhomocysteine (SAH), betaine, choline, 76 and cystathionine, were carried out at the Center of Metabolomics, Baylor Scott & White S3 77 Research Institute using HPLC-MS/MS-based methods,12, 13 with overall intra- and inter-assay 78 CVs of less than 15%.
Fluorescence:Article Title: Metabolic Signature of Arsenic Exposure and Metabolism: The Folic Acid and Creatine Trial
Article Snippet: .. 67 As previously described,8 plasma vitamin B12 was measured using a radio protein-binding assay 68 (SimulTRAC-SNB; MP Biomedicals), with intra- and inter-assay coefficients of variation (CVs) 69 of 6% and 17%, whereas plasma total homocysteine and cysteine concentrations were measured 70 by HPLC with fluorescence detection using the method of Pfeiffer et al,9 with intra- and inter71 assay CVs of 4% and 7% and of 5% and 8%, respectively. .. Plasma dimethylglycine 72 concentrations were measured using HPLC coupled with tandem mass spectrometry (MS/MS) at 73 Division of Nutritional Sciences Cornell University, with overall intra- and inter-assay CVs less 74 than 10%.10, 11 The analyses of 5-methyl-tetrahydrofolate (5-mTHF) and other OCM 75 intermediates, including methionine, SAM, S-adenosylhomocysteine (SAH), betaine, choline, 76 and cystathionine, were carried out at the Center of Metabolomics, Baylor Scott & White S3 77 Research Institute using HPLC-MS/MS-based methods,12, 13 with overall intra- and inter-assay 78 CVs of less than 15%.
RIA Assay:Article Title: The Correlation Between Plasma Vitamin D and Blood Parameters in Prenatal Women
Article Snippet: .. Ferritin and total iron-binding capacity were analyzed by the turbidimetric method; red blood cell (RBC) counts were analyzed using a hematology analyzer; hemoglobin values were analyzed by the sodium lauryl sulfate hemoglobin detection method; iron concentration was tested using UniCel DxC 800 (Beckman Coulter, Brea, CA, USA); plasma folate and vitamin B12 were analyzed using SimulTRAC-SNB radioimmunoassay (MP Biomedicals, Irvine, CA, USA); and plasma vitamin D was analyzed using a Elecsys ® Vitamin D total reagent kit (Roche, Basel, Switzerland). .. Urinary iodine was tested using a spectrophotometer (405 nm) to determine absorbance (HITACHI Spectrometer F-7000, Tokyo, Japan).
|
