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Valiant Co Ltd mci
Mci, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0219550690/Sphingomyelin/10__1165_slash_rcmb__2009___0174oc-89-20-24
Average 90 stars, based on 10 article reviews
mci - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Incubation:

Article Title: Melanoma cell metastasis via P-selectin-mediated activation of acid sphingomyelinase in platelets.
Article Snippet: Metastatic dissemination of cancer cells is one of the hallmarks of malignancy and accounts for approximately 90 % of human cancer deaths.. Within the blood vasculature, tumor cells may aggregate with platelets to form clots, adhere to and spread onto endothelial cells, and finally extravasate to form metastatic colonies.. We have previously shown that sphingolipids play a central role in the interaction of tumor cells with platelets; this interaction is a prerequisite for hematogenous tumor metastasis in at least some tumor models.

Article Title: Regulation of hematogenous tumor metastasis by acid sphingomyelinase
Article Snippet: .. The samples were diluted to 0.1% NP-40 and sonicated three times for 10 s. The samples were incubated for 30 min at 37°C with [ 14 C]sphingomyelin (0.05 mCi per sample, 52 mCi/mmol; MP Biomedicals) that was resuspended after drying in 250 mM sodium acetate (pH 5.0), 1.3 mM EDTA, 0.1% NP-40. ..

Sonication:

Article Title: Lipid storage disorders block lysosomal trafficking by inhibiting a TRP channel and lysosomal calcium release.
Article Snippet: .. Sphingomyelins (Sigma) were dissolved in ‘Tyrode’ or ‘Low pH Tyrode’ solutions by sonication. β-cyclodextrin, filipin and fluoxetine were obtained from Sigma; desipramine was purchased from Santa Cruz; C6-ceramide was obtained from Matreya, LLC; sphingomyelinase was obtained from MP Biomedicals; SF-51 and ML-SA1 were purchased from Princeton BioMolecular Research Inc; PI(3,5)P2 was purchased from Echelon; U18666A was purchased from Enzo; LacCerBODIPY was purchased from Invitrogen, and lysenin and anti-lysenin antibody were purchased from Peptides International. ..

Article Title: Regulation of hematogenous tumor metastasis by acid sphingomyelinase
Article Snippet: .. The samples were diluted to 0.1% NP-40 and sonicated three times for 10 s. The samples were incubated for 30 min at 37°C with [ 14 C]sphingomyelin (0.05 mCi per sample, 52 mCi/mmol; MP Biomedicals) that was resuspended after drying in 250 mM sodium acetate (pH 5.0), 1.3 mM EDTA, 0.1% NP-40. ..

other:

Article Title: Acid Sphingomyelinase Inhibitors Normalize Pulmonary Ceramide and Inflammation in Cystic Fibrosis
Article Snippet: Employing genetic mouse models we have recently shown that ceramide accumulation is critically involved in the pathogenesis of cystic fibrosis (CF) lung disease.. Genetic or systemic inhibition of the acidsphingomyelinase (Asm) isnot feasible for treatmentofpatients or might cause adverse effects.. Thus, a manipulation of ceramide specifically in lungs of CF mice must be developed.



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