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Valiant Co Ltd nahco3
Nahco3, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 160 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/02195497%2E5/Sodium+bicarbonate/pm41271759-469-32-29
Average 96 stars, based on 160 article reviews
nahco3 - by Bioz Stars, 2026-09
96/100 stars

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Lysis:

Article Title: Unraveling the influence of microbial necromass on subsurface microbiomes: metabolite utilization and community dynamics
Article Snippet: .. Thawed pellets were resuspended in 1 ml of 30 mM sodium bicarbonate buffer each and transferred in 1.5 ml aliquots into “Lysis Matrix A” (MP Bio) bead beating tubes and vortexed at maximum power for 20 min. After vortexing, the bead tubes were centrifuged at 10 000 g for 10 min and supernatants were recombined in 15 ml Falcon tubes, filtered through 0.22 μm syringe filters, and stored at -80°C until the incubation’s onset. ..

Incubation:

Article Title: Unraveling the influence of microbial necromass on subsurface microbiomes: metabolite utilization and community dynamics
Article Snippet: .. Thawed pellets were resuspended in 1 ml of 30 mM sodium bicarbonate buffer each and transferred in 1.5 ml aliquots into “Lysis Matrix A” (MP Bio) bead beating tubes and vortexed at maximum power for 20 min. After vortexing, the bead tubes were centrifuged at 10 000 g for 10 min and supernatants were recombined in 15 ml Falcon tubes, filtered through 0.22 μm syringe filters, and stored at -80°C until the incubation’s onset. ..

Article Title: Inhibition of Dormant Lung Cancer Cell Reactivation by Punica Granatum Peel and Dioscorea Nipponica: Involving MYC, SKP2 and p27
Article Snippet: 1 mL of dissolved solution was kept as control and the remaining solution was adjusted to pH 2 with HCl followed by incubation with 5 mg/sample of pepsin from porcine stomach mucosa (P6887, Sigma-Aldrich) for 2 hours in a shaking bath. .. Samples were then neutralized with NaHCO3 to pH 8.0 and incubated with 20 mg/sample of pancreatin from porcine pancreas (193975, MP Biomedicals) and 200 μg/mL of bile extract mixture (B8756, Sigma-Aldrich) for 2 hours in a shaking bath. ..

Article Title: In vitro enzymatic hydrolysis for xylo-oligosaccharide release from wheat-, maize-, maize and wheat distillers dried grains with solubles- and sorghum-based broiler diets
Article Snippet: .. Samples were incubated with shaking at 42°C for 20 min. For the intestinal phase, immediately after the gastric phase, the pH was adjusted to 6 with 1 M NaHCO3, and 50 μL of pancreatin (1 x USP) solution at 40 mg/mL (MP Biomedicals Australia Pty Ltd, Seven Hills, NSW, Australia) was added. ..

Article Title: In vitro enzymatic hydrolysis for xylo-oligosaccharide release from wheat-, maize-, maize and wheat distillers dried grains with solubles- and sorghum-based broiler diets.
Article Snippet: .. Samples were incubated with shaking at 42◦C for 20 min. For the intestinal phase, immediately after the gastric phase, the pH was adjusted to 6 with 1 M NaHCO3, and 50 μL of pancreatin (1 x USP) solution at 40 mg/mL (MP Biomedicals Australia Pty Ltd, Seven Hills, NSW, Australia) was added. ..

Purification:

Article Title: Immunogenicity and efficacy of homologous and heterologous NDV and MVA SARS-CoV-2 vaccines in mice and hamsters.
Article Snippet: Plaque assay with hamster tissues Vero-E6 cells (ATCC,CRL-1586)were seeded in 12-well plates and the next day, confluentmonolayerswere infectedwith serial 10-fold dilutions of viral stock or supernatants of hamster tissue homogenates at 37 °C and 5% CO2 using an inoculum of 200 μL per well. .. Plates were rocked every 10–15min, and after 1 h, inoculum was removed and an overlay consisting of MEM (Lonza) with penicillin/streptomycin (Corning), L-Glutamine (Gibco-Life Technologies), HEPES (Gibco-Life Technologies), BSA (MP Biomedicals), and NaHCO3 supplemented with 0.7% purified agar (Oxoid) and 2% FBS (Peak Serum)was added to eachwell. ..



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