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peroxidase conjugated goat anti rabbit antibody  (Valiant Co Ltd)

 
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    Structured Review

    Valiant Co Ltd peroxidase conjugated goat anti rabbit antibody
    Peroxidase Conjugated Goat Anti Rabbit Antibody, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 407 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/0219537301/Peroxidase/pmc08539567-90-100-104
    Average 95 stars, based on 407 article reviews
    peroxidase conjugated goat anti rabbit antibody - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Dot Blot:

    Article Title: Functional Transport Properties of Human Zinc Transporter 1: Kinetics and pH-Dependency.
    Article Snippet: .. The reaction mixture was analyzed on a dot blot probed with rabbit polyclonal primary antibodies specific for the C-terminal region (E494-L507) of rat ZnT1 (Synaptic Systems, Göttingen, Germany) and peroxidase-conjugated goat polyclonal secondary antibodies to rabbit IgG (MP Biomedicals, Irvine, CA, U.S.A.). ..

    Blocking Assay:

    Article Title: Viral mimic polyinosine-polycytidylic acid potentiates liver injury in trichloroethylene-sensitized mice – viral-chemical interaction as a novel mechanism
    Article Snippet: .. After antigen retrieval and endogenous peroxidase block, tissues were incubated with C3 antibody (MP Biomedicals Headquarters, Santa Ana, CA), C5b-9 antibody (Abcam, Cambridge, MA, USA), factor B antibody (Abcam, Cambridge, MA, USA) overnight at 4°C. .. Thereafter, slides were incubated with horseradish peroxidase-labeled avidin-biotin complex (Dako, Carpinteria, CA, USA).

    Article Title: Heat-killed Bifidobacterium longum LB-P23 ameliorates metabolic dysfunction in high-fat and high-sucrose diet-fed mice through gut microbiota modulation
    Article Snippet: .. Antigen retrieval was performed using 10 mM Tris and 1 mM ethylenediaminetetraacetic acid (EDTA) buffer in a pressure cooker for 30 min. Endogenous peroxidase activity was blocked with 3% hydrogen peroxide for 15 min. After blocking with 4% bovine serum albumin (BSA) in phosphate-buffered saline with tween 20 (PBST) (QBiogene, BSASG100) for 30 min at room temperauter, the sections were incubated with anti-UCP1 primary antibody (Abcam, USA, ab10983, 1:4000) for 1 h, followed by a secondary anti-rabbit antibody (Abcam, USA) for 30 min. Signal detection was performed using 3,3′-diaminobenzidine (DAB, Sigma), and slides were counterstained with Mayer's hematoxylin, dehydrated, and mounted. .. The proportion of UCP1-positive area was quantified using ImageJ software.

    Incubation:

    Article Title: Viral mimic polyinosine-polycytidylic acid potentiates liver injury in trichloroethylene-sensitized mice – viral-chemical interaction as a novel mechanism
    Article Snippet: .. After antigen retrieval and endogenous peroxidase block, tissues were incubated with C3 antibody (MP Biomedicals Headquarters, Santa Ana, CA), C5b-9 antibody (Abcam, Cambridge, MA, USA), factor B antibody (Abcam, Cambridge, MA, USA) overnight at 4°C. .. Thereafter, slides were incubated with horseradish peroxidase-labeled avidin-biotin complex (Dako, Carpinteria, CA, USA).

    Article Title: Heat-killed Bifidobacterium longum LB-P23 ameliorates metabolic dysfunction in high-fat and high-sucrose diet-fed mice through gut microbiota modulation
    Article Snippet: .. Antigen retrieval was performed using 10 mM Tris and 1 mM ethylenediaminetetraacetic acid (EDTA) buffer in a pressure cooker for 30 min. Endogenous peroxidase activity was blocked with 3% hydrogen peroxide for 15 min. After blocking with 4% bovine serum albumin (BSA) in phosphate-buffered saline with tween 20 (PBST) (QBiogene, BSASG100) for 30 min at room temperauter, the sections were incubated with anti-UCP1 primary antibody (Abcam, USA, ab10983, 1:4000) for 1 h, followed by a secondary anti-rabbit antibody (Abcam, USA) for 30 min. Signal detection was performed using 3,3′-diaminobenzidine (DAB, Sigma), and slides were counterstained with Mayer's hematoxylin, dehydrated, and mounted. .. The proportion of UCP1-positive area was quantified using ImageJ software.

    Activity Assay:

    Article Title: Heat-killed Bifidobacterium longum LB-P23 ameliorates metabolic dysfunction in high-fat and high-sucrose diet-fed mice through gut microbiota modulation
    Article Snippet: .. Antigen retrieval was performed using 10 mM Tris and 1 mM ethylenediaminetetraacetic acid (EDTA) buffer in a pressure cooker for 30 min. Endogenous peroxidase activity was blocked with 3% hydrogen peroxide for 15 min. After blocking with 4% bovine serum albumin (BSA) in phosphate-buffered saline with tween 20 (PBST) (QBiogene, BSASG100) for 30 min at room temperauter, the sections were incubated with anti-UCP1 primary antibody (Abcam, USA, ab10983, 1:4000) for 1 h, followed by a secondary anti-rabbit antibody (Abcam, USA) for 30 min. Signal detection was performed using 3,3′-diaminobenzidine (DAB, Sigma), and slides were counterstained with Mayer's hematoxylin, dehydrated, and mounted. .. The proportion of UCP1-positive area was quantified using ImageJ software.

    Saline:

    Article Title: Heat-killed Bifidobacterium longum LB-P23 ameliorates metabolic dysfunction in high-fat and high-sucrose diet-fed mice through gut microbiota modulation
    Article Snippet: .. Antigen retrieval was performed using 10 mM Tris and 1 mM ethylenediaminetetraacetic acid (EDTA) buffer in a pressure cooker for 30 min. Endogenous peroxidase activity was blocked with 3% hydrogen peroxide for 15 min. After blocking with 4% bovine serum albumin (BSA) in phosphate-buffered saline with tween 20 (PBST) (QBiogene, BSASG100) for 30 min at room temperauter, the sections were incubated with anti-UCP1 primary antibody (Abcam, USA, ab10983, 1:4000) for 1 h, followed by a secondary anti-rabbit antibody (Abcam, USA) for 30 min. Signal detection was performed using 3,3′-diaminobenzidine (DAB, Sigma), and slides were counterstained with Mayer's hematoxylin, dehydrated, and mounted. .. The proportion of UCP1-positive area was quantified using ImageJ software.

    Virus:

    Article Title: The free fatty acid receptor GPR164 maintains intestinal homeostasis and barrier function.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models C57BL/6JJmsSlc (M. musculus) Japan SLC GPR164−/− (M. musculus) This study Table EV1 Recombinant DNA pcDNA3.1_HA-Or51e1 This study Materials and methods pcDNA3.1_Rtp1S This study Materials and methods pcDNA3.1_Rtp4 This study Materials and methods peSpCAS9(1.1)-2xsgRNA Addgene #80768 eSpCAS9(1.1)-2xsgRNA and pDonor-tBFPNLS-Neo Addgene #80766 Antibodies Anti-p53 antibody Santa Cruz sc-126 Anti-tubulin antibody Sigma T5168 Anti-β-catenin Proteintech 51067-2-AP Anti-Ki67 antibody Proteintech 28074-1-AP Oligonucleotides and other sequence-based reagents Genotyping primers This study Fig. EV1 Cloning primers This study Materials and methods qPCR primers This study Table EV1 16S rRNA sequencing primer This study Materials and methods Bacterial primers This study Materials and methods 12 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/resource Reference or source Identifier or catalog number Chemicals, enzymes and other reagents CE-2 Japan CLEA 30088828 HFD Research diet D12492 FITC-dextran Sigma 60842-46-8 DMEM Wako 043-30085 FBS Cosmo Bio S-FBS-CO015 PenStrept Gibco 15140-122 Lipofectamine 2000 Invitrogen 11668019 G418 Wako 074-06801 Culture insert Falcon 353095 Sodium butyrate Tokyo Chemical Industry 156-54-7 Sodium palmitate Tokyo Chemical Industry 408-35-5 RNAiso plus TAKARA 9109 Moloney murine leukemia virus reverse transcriptase Invitrogen 28025013 3-isobutyl 1-methylxantine Sigma 28822-58-4 cAMP EIA kit Cayman Chemical 581001 Folskolin Sigma 66575-29-9 Crystal violet Wako 031-04852 Alexa Fluor 488 Invitrogen A11008 Goat anti-rabbit IgG antibody (H+ L), peroxidase Vector Laboratories PI-1000 Diaminobenzidine DOJINDO 349-00903 Sodium citrate buffer KANTO KAGAKU 73114 Carnoy’s solution Wako 034-17711 Alcian blue-PAS stain kit Scy Tek APS-1 FastDNA SPIN kit MP Biomedicals 116560200- CF AMPure XP Beckman Coulter A63881 RNeasy mini kit Qiagen 74904 RNA 6000 Nano kit Agilent Technologies 5067-1511 NEBNext® UltraTM II Directional RNA Library Prep Kit Illumina E7760S NEBNext Multiplex Oligos for Illumina Illumina E7335S IntestiCult organoid growth medium Veritas ST-06010 Software CRISPOR http://crispor.tefor.net trimmomatic (v0.39) https://github.com/usadellab/ Trimmomatic/releases/ download/v0.39/Trimmomatic0.39.zip FastQC (v0.11.8.-2) https://sourceforge.net/ projects/fastqc.mirror/files/ v0.11.8/ Reagent/resource Reference or source Identifier or catalog number STAR (v2.7.10a) https://github.com/alexdobin/ STAR/releases/download/ 2.7.11b/STAR_2.7.11b.zip GraphPad Prism https://www.graphpad.com/ Primer 3 https://primer3.ut.ee/ Other Millicell-ERS system Millipore MERS00002 StepOne real-time PCR system Applied Biosystems SYBR Premix Ex Taq II TAKARA RR820A Preparation of crosssections Kyoto Institute of Nutrition & Pathology, Inc GCMS-QP2010 Ultra system Shimazu MiSeq platform Illumina Agilent 2100 Bioanalyzer Agilent Technologies NovaSeq 6000 platform Illumina Kyoto Encyclopedia of Genes and Genomes (KEGG) database http://www.genome.jp/kegg/ Fluorescence microscope Keyence BZ-X710 .. Male C57BL/6J mice were purchased from Japan SLC and Or51e1 gene knockout (Gpr164−/−) mice were generated by using the CRISPR/Cas9 system in wild-type C57BL/6J zygotes (Fig. EV1D–F).

    Reverse Transcription:

    Article Title: The free fatty acid receptor GPR164 maintains intestinal homeostasis and barrier function.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models C57BL/6JJmsSlc (M. musculus) Japan SLC GPR164−/− (M. musculus) This study Table EV1 Recombinant DNA pcDNA3.1_HA-Or51e1 This study Materials and methods pcDNA3.1_Rtp1S This study Materials and methods pcDNA3.1_Rtp4 This study Materials and methods peSpCAS9(1.1)-2xsgRNA Addgene #80768 eSpCAS9(1.1)-2xsgRNA and pDonor-tBFPNLS-Neo Addgene #80766 Antibodies Anti-p53 antibody Santa Cruz sc-126 Anti-tubulin antibody Sigma T5168 Anti-β-catenin Proteintech 51067-2-AP Anti-Ki67 antibody Proteintech 28074-1-AP Oligonucleotides and other sequence-based reagents Genotyping primers This study Fig. EV1 Cloning primers This study Materials and methods qPCR primers This study Table EV1 16S rRNA sequencing primer This study Materials and methods Bacterial primers This study Materials and methods 12 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/resource Reference or source Identifier or catalog number Chemicals, enzymes and other reagents CE-2 Japan CLEA 30088828 HFD Research diet D12492 FITC-dextran Sigma 60842-46-8 DMEM Wako 043-30085 FBS Cosmo Bio S-FBS-CO015 PenStrept Gibco 15140-122 Lipofectamine 2000 Invitrogen 11668019 G418 Wako 074-06801 Culture insert Falcon 353095 Sodium butyrate Tokyo Chemical Industry 156-54-7 Sodium palmitate Tokyo Chemical Industry 408-35-5 RNAiso plus TAKARA 9109 Moloney murine leukemia virus reverse transcriptase Invitrogen 28025013 3-isobutyl 1-methylxantine Sigma 28822-58-4 cAMP EIA kit Cayman Chemical 581001 Folskolin Sigma 66575-29-9 Crystal violet Wako 031-04852 Alexa Fluor 488 Invitrogen A11008 Goat anti-rabbit IgG antibody (H+ L), peroxidase Vector Laboratories PI-1000 Diaminobenzidine DOJINDO 349-00903 Sodium citrate buffer KANTO KAGAKU 73114 Carnoy’s solution Wako 034-17711 Alcian blue-PAS stain kit Scy Tek APS-1 FastDNA SPIN kit MP Biomedicals 116560200- CF AMPure XP Beckman Coulter A63881 RNeasy mini kit Qiagen 74904 RNA 6000 Nano kit Agilent Technologies 5067-1511 NEBNext® UltraTM II Directional RNA Library Prep Kit Illumina E7760S NEBNext Multiplex Oligos for Illumina Illumina E7335S IntestiCult organoid growth medium Veritas ST-06010 Software CRISPOR http://crispor.tefor.net trimmomatic (v0.39) https://github.com/usadellab/ Trimmomatic/releases/ download/v0.39/Trimmomatic0.39.zip FastQC (v0.11.8.-2) https://sourceforge.net/ projects/fastqc.mirror/files/ v0.11.8/ Reagent/resource Reference or source Identifier or catalog number STAR (v2.7.10a) https://github.com/alexdobin/ STAR/releases/download/ 2.7.11b/STAR_2.7.11b.zip GraphPad Prism https://www.graphpad.com/ Primer 3 https://primer3.ut.ee/ Other Millicell-ERS system Millipore MERS00002 StepOne real-time PCR system Applied Biosystems SYBR Premix Ex Taq II TAKARA RR820A Preparation of crosssections Kyoto Institute of Nutrition & Pathology, Inc GCMS-QP2010 Ultra system Shimazu MiSeq platform Illumina Agilent 2100 Bioanalyzer Agilent Technologies NovaSeq 6000 platform Illumina Kyoto Encyclopedia of Genes and Genomes (KEGG) database http://www.genome.jp/kegg/ Fluorescence microscope Keyence BZ-X710 .. Male C57BL/6J mice were purchased from Japan SLC and Or51e1 gene knockout (Gpr164−/−) mice were generated by using the CRISPR/Cas9 system in wild-type C57BL/6J zygotes (Fig. EV1D–F).

    Enzyme-linked Immunosorbent Assay:

    Article Title: The free fatty acid receptor GPR164 maintains intestinal homeostasis and barrier function.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models C57BL/6JJmsSlc (M. musculus) Japan SLC GPR164−/− (M. musculus) This study Table EV1 Recombinant DNA pcDNA3.1_HA-Or51e1 This study Materials and methods pcDNA3.1_Rtp1S This study Materials and methods pcDNA3.1_Rtp4 This study Materials and methods peSpCAS9(1.1)-2xsgRNA Addgene #80768 eSpCAS9(1.1)-2xsgRNA and pDonor-tBFPNLS-Neo Addgene #80766 Antibodies Anti-p53 antibody Santa Cruz sc-126 Anti-tubulin antibody Sigma T5168 Anti-β-catenin Proteintech 51067-2-AP Anti-Ki67 antibody Proteintech 28074-1-AP Oligonucleotides and other sequence-based reagents Genotyping primers This study Fig. EV1 Cloning primers This study Materials and methods qPCR primers This study Table EV1 16S rRNA sequencing primer This study Materials and methods Bacterial primers This study Materials and methods 12 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/resource Reference or source Identifier or catalog number Chemicals, enzymes and other reagents CE-2 Japan CLEA 30088828 HFD Research diet D12492 FITC-dextran Sigma 60842-46-8 DMEM Wako 043-30085 FBS Cosmo Bio S-FBS-CO015 PenStrept Gibco 15140-122 Lipofectamine 2000 Invitrogen 11668019 G418 Wako 074-06801 Culture insert Falcon 353095 Sodium butyrate Tokyo Chemical Industry 156-54-7 Sodium palmitate Tokyo Chemical Industry 408-35-5 RNAiso plus TAKARA 9109 Moloney murine leukemia virus reverse transcriptase Invitrogen 28025013 3-isobutyl 1-methylxantine Sigma 28822-58-4 cAMP EIA kit Cayman Chemical 581001 Folskolin Sigma 66575-29-9 Crystal violet Wako 031-04852 Alexa Fluor 488 Invitrogen A11008 Goat anti-rabbit IgG antibody (H+ L), peroxidase Vector Laboratories PI-1000 Diaminobenzidine DOJINDO 349-00903 Sodium citrate buffer KANTO KAGAKU 73114 Carnoy’s solution Wako 034-17711 Alcian blue-PAS stain kit Scy Tek APS-1 FastDNA SPIN kit MP Biomedicals 116560200- CF AMPure XP Beckman Coulter A63881 RNeasy mini kit Qiagen 74904 RNA 6000 Nano kit Agilent Technologies 5067-1511 NEBNext® UltraTM II Directional RNA Library Prep Kit Illumina E7760S NEBNext Multiplex Oligos for Illumina Illumina E7335S IntestiCult organoid growth medium Veritas ST-06010 Software CRISPOR http://crispor.tefor.net trimmomatic (v0.39) https://github.com/usadellab/ Trimmomatic/releases/ download/v0.39/Trimmomatic0.39.zip FastQC (v0.11.8.-2) https://sourceforge.net/ projects/fastqc.mirror/files/ v0.11.8/ Reagent/resource Reference or source Identifier or catalog number STAR (v2.7.10a) https://github.com/alexdobin/ STAR/releases/download/ 2.7.11b/STAR_2.7.11b.zip GraphPad Prism https://www.graphpad.com/ Primer 3 https://primer3.ut.ee/ Other Millicell-ERS system Millipore MERS00002 StepOne real-time PCR system Applied Biosystems SYBR Premix Ex Taq II TAKARA RR820A Preparation of crosssections Kyoto Institute of Nutrition & Pathology, Inc GCMS-QP2010 Ultra system Shimazu MiSeq platform Illumina Agilent 2100 Bioanalyzer Agilent Technologies NovaSeq 6000 platform Illumina Kyoto Encyclopedia of Genes and Genomes (KEGG) database http://www.genome.jp/kegg/ Fluorescence microscope Keyence BZ-X710 .. Male C57BL/6J mice were purchased from Japan SLC and Or51e1 gene knockout (Gpr164−/−) mice were generated by using the CRISPR/Cas9 system in wild-type C57BL/6J zygotes (Fig. EV1D–F).

    Staining:

    Article Title: The free fatty acid receptor GPR164 maintains intestinal homeostasis and barrier function.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models C57BL/6JJmsSlc (M. musculus) Japan SLC GPR164−/− (M. musculus) This study Table EV1 Recombinant DNA pcDNA3.1_HA-Or51e1 This study Materials and methods pcDNA3.1_Rtp1S This study Materials and methods pcDNA3.1_Rtp4 This study Materials and methods peSpCAS9(1.1)-2xsgRNA Addgene #80768 eSpCAS9(1.1)-2xsgRNA and pDonor-tBFPNLS-Neo Addgene #80766 Antibodies Anti-p53 antibody Santa Cruz sc-126 Anti-tubulin antibody Sigma T5168 Anti-β-catenin Proteintech 51067-2-AP Anti-Ki67 antibody Proteintech 28074-1-AP Oligonucleotides and other sequence-based reagents Genotyping primers This study Fig. EV1 Cloning primers This study Materials and methods qPCR primers This study Table EV1 16S rRNA sequencing primer This study Materials and methods Bacterial primers This study Materials and methods 12 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/resource Reference or source Identifier or catalog number Chemicals, enzymes and other reagents CE-2 Japan CLEA 30088828 HFD Research diet D12492 FITC-dextran Sigma 60842-46-8 DMEM Wako 043-30085 FBS Cosmo Bio S-FBS-CO015 PenStrept Gibco 15140-122 Lipofectamine 2000 Invitrogen 11668019 G418 Wako 074-06801 Culture insert Falcon 353095 Sodium butyrate Tokyo Chemical Industry 156-54-7 Sodium palmitate Tokyo Chemical Industry 408-35-5 RNAiso plus TAKARA 9109 Moloney murine leukemia virus reverse transcriptase Invitrogen 28025013 3-isobutyl 1-methylxantine Sigma 28822-58-4 cAMP EIA kit Cayman Chemical 581001 Folskolin Sigma 66575-29-9 Crystal violet Wako 031-04852 Alexa Fluor 488 Invitrogen A11008 Goat anti-rabbit IgG antibody (H+ L), peroxidase Vector Laboratories PI-1000 Diaminobenzidine DOJINDO 349-00903 Sodium citrate buffer KANTO KAGAKU 73114 Carnoy’s solution Wako 034-17711 Alcian blue-PAS stain kit Scy Tek APS-1 FastDNA SPIN kit MP Biomedicals 116560200- CF AMPure XP Beckman Coulter A63881 RNeasy mini kit Qiagen 74904 RNA 6000 Nano kit Agilent Technologies 5067-1511 NEBNext® UltraTM II Directional RNA Library Prep Kit Illumina E7760S NEBNext Multiplex Oligos for Illumina Illumina E7335S IntestiCult organoid growth medium Veritas ST-06010 Software CRISPOR http://crispor.tefor.net trimmomatic (v0.39) https://github.com/usadellab/ Trimmomatic/releases/ download/v0.39/Trimmomatic0.39.zip FastQC (v0.11.8.-2) https://sourceforge.net/ projects/fastqc.mirror/files/ v0.11.8/ Reagent/resource Reference or source Identifier or catalog number STAR (v2.7.10a) https://github.com/alexdobin/ STAR/releases/download/ 2.7.11b/STAR_2.7.11b.zip GraphPad Prism https://www.graphpad.com/ Primer 3 https://primer3.ut.ee/ Other Millicell-ERS system Millipore MERS00002 StepOne real-time PCR system Applied Biosystems SYBR Premix Ex Taq II TAKARA RR820A Preparation of crosssections Kyoto Institute of Nutrition & Pathology, Inc GCMS-QP2010 Ultra system Shimazu MiSeq platform Illumina Agilent 2100 Bioanalyzer Agilent Technologies NovaSeq 6000 platform Illumina Kyoto Encyclopedia of Genes and Genomes (KEGG) database http://www.genome.jp/kegg/ Fluorescence microscope Keyence BZ-X710 .. Male C57BL/6J mice were purchased from Japan SLC and Or51e1 gene knockout (Gpr164−/−) mice were generated by using the CRISPR/Cas9 system in wild-type C57BL/6J zygotes (Fig. EV1D–F).

    Multiplex Assay:

    Article Title: The free fatty acid receptor GPR164 maintains intestinal homeostasis and barrier function.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models C57BL/6JJmsSlc (M. musculus) Japan SLC GPR164−/− (M. musculus) This study Table EV1 Recombinant DNA pcDNA3.1_HA-Or51e1 This study Materials and methods pcDNA3.1_Rtp1S This study Materials and methods pcDNA3.1_Rtp4 This study Materials and methods peSpCAS9(1.1)-2xsgRNA Addgene #80768 eSpCAS9(1.1)-2xsgRNA and pDonor-tBFPNLS-Neo Addgene #80766 Antibodies Anti-p53 antibody Santa Cruz sc-126 Anti-tubulin antibody Sigma T5168 Anti-β-catenin Proteintech 51067-2-AP Anti-Ki67 antibody Proteintech 28074-1-AP Oligonucleotides and other sequence-based reagents Genotyping primers This study Fig. EV1 Cloning primers This study Materials and methods qPCR primers This study Table EV1 16S rRNA sequencing primer This study Materials and methods Bacterial primers This study Materials and methods 12 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/resource Reference or source Identifier or catalog number Chemicals, enzymes and other reagents CE-2 Japan CLEA 30088828 HFD Research diet D12492 FITC-dextran Sigma 60842-46-8 DMEM Wako 043-30085 FBS Cosmo Bio S-FBS-CO015 PenStrept Gibco 15140-122 Lipofectamine 2000 Invitrogen 11668019 G418 Wako 074-06801 Culture insert Falcon 353095 Sodium butyrate Tokyo Chemical Industry 156-54-7 Sodium palmitate Tokyo Chemical Industry 408-35-5 RNAiso plus TAKARA 9109 Moloney murine leukemia virus reverse transcriptase Invitrogen 28025013 3-isobutyl 1-methylxantine Sigma 28822-58-4 cAMP EIA kit Cayman Chemical 581001 Folskolin Sigma 66575-29-9 Crystal violet Wako 031-04852 Alexa Fluor 488 Invitrogen A11008 Goat anti-rabbit IgG antibody (H+ L), peroxidase Vector Laboratories PI-1000 Diaminobenzidine DOJINDO 349-00903 Sodium citrate buffer KANTO KAGAKU 73114 Carnoy’s solution Wako 034-17711 Alcian blue-PAS stain kit Scy Tek APS-1 FastDNA SPIN kit MP Biomedicals 116560200- CF AMPure XP Beckman Coulter A63881 RNeasy mini kit Qiagen 74904 RNA 6000 Nano kit Agilent Technologies 5067-1511 NEBNext® UltraTM II Directional RNA Library Prep Kit Illumina E7760S NEBNext Multiplex Oligos for Illumina Illumina E7335S IntestiCult organoid growth medium Veritas ST-06010 Software CRISPOR http://crispor.tefor.net trimmomatic (v0.39) https://github.com/usadellab/ Trimmomatic/releases/ download/v0.39/Trimmomatic0.39.zip FastQC (v0.11.8.-2) https://sourceforge.net/ projects/fastqc.mirror/files/ v0.11.8/ Reagent/resource Reference or source Identifier or catalog number STAR (v2.7.10a) https://github.com/alexdobin/ STAR/releases/download/ 2.7.11b/STAR_2.7.11b.zip GraphPad Prism https://www.graphpad.com/ Primer 3 https://primer3.ut.ee/ Other Millicell-ERS system Millipore MERS00002 StepOne real-time PCR system Applied Biosystems SYBR Premix Ex Taq II TAKARA RR820A Preparation of crosssections Kyoto Institute of Nutrition & Pathology, Inc GCMS-QP2010 Ultra system Shimazu MiSeq platform Illumina Agilent 2100 Bioanalyzer Agilent Technologies NovaSeq 6000 platform Illumina Kyoto Encyclopedia of Genes and Genomes (KEGG) database http://www.genome.jp/kegg/ Fluorescence microscope Keyence BZ-X710 .. Male C57BL/6J mice were purchased from Japan SLC and Or51e1 gene knockout (Gpr164−/−) mice were generated by using the CRISPR/Cas9 system in wild-type C57BL/6J zygotes (Fig. EV1D–F).

    Software:

    Article Title: The free fatty acid receptor GPR164 maintains intestinal homeostasis and barrier function.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models C57BL/6JJmsSlc (M. musculus) Japan SLC GPR164−/− (M. musculus) This study Table EV1 Recombinant DNA pcDNA3.1_HA-Or51e1 This study Materials and methods pcDNA3.1_Rtp1S This study Materials and methods pcDNA3.1_Rtp4 This study Materials and methods peSpCAS9(1.1)-2xsgRNA Addgene #80768 eSpCAS9(1.1)-2xsgRNA and pDonor-tBFPNLS-Neo Addgene #80766 Antibodies Anti-p53 antibody Santa Cruz sc-126 Anti-tubulin antibody Sigma T5168 Anti-β-catenin Proteintech 51067-2-AP Anti-Ki67 antibody Proteintech 28074-1-AP Oligonucleotides and other sequence-based reagents Genotyping primers This study Fig. EV1 Cloning primers This study Materials and methods qPCR primers This study Table EV1 16S rRNA sequencing primer This study Materials and methods Bacterial primers This study Materials and methods 12 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/resource Reference or source Identifier or catalog number Chemicals, enzymes and other reagents CE-2 Japan CLEA 30088828 HFD Research diet D12492 FITC-dextran Sigma 60842-46-8 DMEM Wako 043-30085 FBS Cosmo Bio S-FBS-CO015 PenStrept Gibco 15140-122 Lipofectamine 2000 Invitrogen 11668019 G418 Wako 074-06801 Culture insert Falcon 353095 Sodium butyrate Tokyo Chemical Industry 156-54-7 Sodium palmitate Tokyo Chemical Industry 408-35-5 RNAiso plus TAKARA 9109 Moloney murine leukemia virus reverse transcriptase Invitrogen 28025013 3-isobutyl 1-methylxantine Sigma 28822-58-4 cAMP EIA kit Cayman Chemical 581001 Folskolin Sigma 66575-29-9 Crystal violet Wako 031-04852 Alexa Fluor 488 Invitrogen A11008 Goat anti-rabbit IgG antibody (H+ L), peroxidase Vector Laboratories PI-1000 Diaminobenzidine DOJINDO 349-00903 Sodium citrate buffer KANTO KAGAKU 73114 Carnoy’s solution Wako 034-17711 Alcian blue-PAS stain kit Scy Tek APS-1 FastDNA SPIN kit MP Biomedicals 116560200- CF AMPure XP Beckman Coulter A63881 RNeasy mini kit Qiagen 74904 RNA 6000 Nano kit Agilent Technologies 5067-1511 NEBNext® UltraTM II Directional RNA Library Prep Kit Illumina E7760S NEBNext Multiplex Oligos for Illumina Illumina E7335S IntestiCult organoid growth medium Veritas ST-06010 Software CRISPOR http://crispor.tefor.net trimmomatic (v0.39) https://github.com/usadellab/ Trimmomatic/releases/ download/v0.39/Trimmomatic0.39.zip FastQC (v0.11.8.-2) https://sourceforge.net/ projects/fastqc.mirror/files/ v0.11.8/ Reagent/resource Reference or source Identifier or catalog number STAR (v2.7.10a) https://github.com/alexdobin/ STAR/releases/download/ 2.7.11b/STAR_2.7.11b.zip GraphPad Prism https://www.graphpad.com/ Primer 3 https://primer3.ut.ee/ Other Millicell-ERS system Millipore MERS00002 StepOne real-time PCR system Applied Biosystems SYBR Premix Ex Taq II TAKARA RR820A Preparation of crosssections Kyoto Institute of Nutrition & Pathology, Inc GCMS-QP2010 Ultra system Shimazu MiSeq platform Illumina Agilent 2100 Bioanalyzer Agilent Technologies NovaSeq 6000 platform Illumina Kyoto Encyclopedia of Genes and Genomes (KEGG) database http://www.genome.jp/kegg/ Fluorescence microscope Keyence BZ-X710 .. Male C57BL/6J mice were purchased from Japan SLC and Or51e1 gene knockout (Gpr164−/−) mice were generated by using the CRISPR/Cas9 system in wild-type C57BL/6J zygotes (Fig. EV1D–F).

    Real-time Polymerase Chain Reaction:

    Article Title: The free fatty acid receptor GPR164 maintains intestinal homeostasis and barrier function.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models C57BL/6JJmsSlc (M. musculus) Japan SLC GPR164−/− (M. musculus) This study Table EV1 Recombinant DNA pcDNA3.1_HA-Or51e1 This study Materials and methods pcDNA3.1_Rtp1S This study Materials and methods pcDNA3.1_Rtp4 This study Materials and methods peSpCAS9(1.1)-2xsgRNA Addgene #80768 eSpCAS9(1.1)-2xsgRNA and pDonor-tBFPNLS-Neo Addgene #80766 Antibodies Anti-p53 antibody Santa Cruz sc-126 Anti-tubulin antibody Sigma T5168 Anti-β-catenin Proteintech 51067-2-AP Anti-Ki67 antibody Proteintech 28074-1-AP Oligonucleotides and other sequence-based reagents Genotyping primers This study Fig. EV1 Cloning primers This study Materials and methods qPCR primers This study Table EV1 16S rRNA sequencing primer This study Materials and methods Bacterial primers This study Materials and methods 12 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/resource Reference or source Identifier or catalog number Chemicals, enzymes and other reagents CE-2 Japan CLEA 30088828 HFD Research diet D12492 FITC-dextran Sigma 60842-46-8 DMEM Wako 043-30085 FBS Cosmo Bio S-FBS-CO015 PenStrept Gibco 15140-122 Lipofectamine 2000 Invitrogen 11668019 G418 Wako 074-06801 Culture insert Falcon 353095 Sodium butyrate Tokyo Chemical Industry 156-54-7 Sodium palmitate Tokyo Chemical Industry 408-35-5 RNAiso plus TAKARA 9109 Moloney murine leukemia virus reverse transcriptase Invitrogen 28025013 3-isobutyl 1-methylxantine Sigma 28822-58-4 cAMP EIA kit Cayman Chemical 581001 Folskolin Sigma 66575-29-9 Crystal violet Wako 031-04852 Alexa Fluor 488 Invitrogen A11008 Goat anti-rabbit IgG antibody (H+ L), peroxidase Vector Laboratories PI-1000 Diaminobenzidine DOJINDO 349-00903 Sodium citrate buffer KANTO KAGAKU 73114 Carnoy’s solution Wako 034-17711 Alcian blue-PAS stain kit Scy Tek APS-1 FastDNA SPIN kit MP Biomedicals 116560200- CF AMPure XP Beckman Coulter A63881 RNeasy mini kit Qiagen 74904 RNA 6000 Nano kit Agilent Technologies 5067-1511 NEBNext® UltraTM II Directional RNA Library Prep Kit Illumina E7760S NEBNext Multiplex Oligos for Illumina Illumina E7335S IntestiCult organoid growth medium Veritas ST-06010 Software CRISPOR http://crispor.tefor.net trimmomatic (v0.39) https://github.com/usadellab/ Trimmomatic/releases/ download/v0.39/Trimmomatic0.39.zip FastQC (v0.11.8.-2) https://sourceforge.net/ projects/fastqc.mirror/files/ v0.11.8/ Reagent/resource Reference or source Identifier or catalog number STAR (v2.7.10a) https://github.com/alexdobin/ STAR/releases/download/ 2.7.11b/STAR_2.7.11b.zip GraphPad Prism https://www.graphpad.com/ Primer 3 https://primer3.ut.ee/ Other Millicell-ERS system Millipore MERS00002 StepOne real-time PCR system Applied Biosystems SYBR Premix Ex Taq II TAKARA RR820A Preparation of crosssections Kyoto Institute of Nutrition & Pathology, Inc GCMS-QP2010 Ultra system Shimazu MiSeq platform Illumina Agilent 2100 Bioanalyzer Agilent Technologies NovaSeq 6000 platform Illumina Kyoto Encyclopedia of Genes and Genomes (KEGG) database http://www.genome.jp/kegg/ Fluorescence microscope Keyence BZ-X710 .. Male C57BL/6J mice were purchased from Japan SLC and Or51e1 gene knockout (Gpr164−/−) mice were generated by using the CRISPR/Cas9 system in wild-type C57BL/6J zygotes (Fig. EV1D–F).

    Fluorescence:

    Article Title: The free fatty acid receptor GPR164 maintains intestinal homeostasis and barrier function.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models C57BL/6JJmsSlc (M. musculus) Japan SLC GPR164−/− (M. musculus) This study Table EV1 Recombinant DNA pcDNA3.1_HA-Or51e1 This study Materials and methods pcDNA3.1_Rtp1S This study Materials and methods pcDNA3.1_Rtp4 This study Materials and methods peSpCAS9(1.1)-2xsgRNA Addgene #80768 eSpCAS9(1.1)-2xsgRNA and pDonor-tBFPNLS-Neo Addgene #80766 Antibodies Anti-p53 antibody Santa Cruz sc-126 Anti-tubulin antibody Sigma T5168 Anti-β-catenin Proteintech 51067-2-AP Anti-Ki67 antibody Proteintech 28074-1-AP Oligonucleotides and other sequence-based reagents Genotyping primers This study Fig. EV1 Cloning primers This study Materials and methods qPCR primers This study Table EV1 16S rRNA sequencing primer This study Materials and methods Bacterial primers This study Materials and methods 12 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/resource Reference or source Identifier or catalog number Chemicals, enzymes and other reagents CE-2 Japan CLEA 30088828 HFD Research diet D12492 FITC-dextran Sigma 60842-46-8 DMEM Wako 043-30085 FBS Cosmo Bio S-FBS-CO015 PenStrept Gibco 15140-122 Lipofectamine 2000 Invitrogen 11668019 G418 Wako 074-06801 Culture insert Falcon 353095 Sodium butyrate Tokyo Chemical Industry 156-54-7 Sodium palmitate Tokyo Chemical Industry 408-35-5 RNAiso plus TAKARA 9109 Moloney murine leukemia virus reverse transcriptase Invitrogen 28025013 3-isobutyl 1-methylxantine Sigma 28822-58-4 cAMP EIA kit Cayman Chemical 581001 Folskolin Sigma 66575-29-9 Crystal violet Wako 031-04852 Alexa Fluor 488 Invitrogen A11008 Goat anti-rabbit IgG antibody (H+ L), peroxidase Vector Laboratories PI-1000 Diaminobenzidine DOJINDO 349-00903 Sodium citrate buffer KANTO KAGAKU 73114 Carnoy’s solution Wako 034-17711 Alcian blue-PAS stain kit Scy Tek APS-1 FastDNA SPIN kit MP Biomedicals 116560200- CF AMPure XP Beckman Coulter A63881 RNeasy mini kit Qiagen 74904 RNA 6000 Nano kit Agilent Technologies 5067-1511 NEBNext® UltraTM II Directional RNA Library Prep Kit Illumina E7760S NEBNext Multiplex Oligos for Illumina Illumina E7335S IntestiCult organoid growth medium Veritas ST-06010 Software CRISPOR http://crispor.tefor.net trimmomatic (v0.39) https://github.com/usadellab/ Trimmomatic/releases/ download/v0.39/Trimmomatic0.39.zip FastQC (v0.11.8.-2) https://sourceforge.net/ projects/fastqc.mirror/files/ v0.11.8/ Reagent/resource Reference or source Identifier or catalog number STAR (v2.7.10a) https://github.com/alexdobin/ STAR/releases/download/ 2.7.11b/STAR_2.7.11b.zip GraphPad Prism https://www.graphpad.com/ Primer 3 https://primer3.ut.ee/ Other Millicell-ERS system Millipore MERS00002 StepOne real-time PCR system Applied Biosystems SYBR Premix Ex Taq II TAKARA RR820A Preparation of crosssections Kyoto Institute of Nutrition & Pathology, Inc GCMS-QP2010 Ultra system Shimazu MiSeq platform Illumina Agilent 2100 Bioanalyzer Agilent Technologies NovaSeq 6000 platform Illumina Kyoto Encyclopedia of Genes and Genomes (KEGG) database http://www.genome.jp/kegg/ Fluorescence microscope Keyence BZ-X710 .. Male C57BL/6J mice were purchased from Japan SLC and Or51e1 gene knockout (Gpr164−/−) mice were generated by using the CRISPR/Cas9 system in wild-type C57BL/6J zygotes (Fig. EV1D–F).

    Microscopy:

    Article Title: The free fatty acid receptor GPR164 maintains intestinal homeostasis and barrier function.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models C57BL/6JJmsSlc (M. musculus) Japan SLC GPR164−/− (M. musculus) This study Table EV1 Recombinant DNA pcDNA3.1_HA-Or51e1 This study Materials and methods pcDNA3.1_Rtp1S This study Materials and methods pcDNA3.1_Rtp4 This study Materials and methods peSpCAS9(1.1)-2xsgRNA Addgene #80768 eSpCAS9(1.1)-2xsgRNA and pDonor-tBFPNLS-Neo Addgene #80766 Antibodies Anti-p53 antibody Santa Cruz sc-126 Anti-tubulin antibody Sigma T5168 Anti-β-catenin Proteintech 51067-2-AP Anti-Ki67 antibody Proteintech 28074-1-AP Oligonucleotides and other sequence-based reagents Genotyping primers This study Fig. EV1 Cloning primers This study Materials and methods qPCR primers This study Table EV1 16S rRNA sequencing primer This study Materials and methods Bacterial primers This study Materials and methods 12 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/resource Reference or source Identifier or catalog number Chemicals, enzymes and other reagents CE-2 Japan CLEA 30088828 HFD Research diet D12492 FITC-dextran Sigma 60842-46-8 DMEM Wako 043-30085 FBS Cosmo Bio S-FBS-CO015 PenStrept Gibco 15140-122 Lipofectamine 2000 Invitrogen 11668019 G418 Wako 074-06801 Culture insert Falcon 353095 Sodium butyrate Tokyo Chemical Industry 156-54-7 Sodium palmitate Tokyo Chemical Industry 408-35-5 RNAiso plus TAKARA 9109 Moloney murine leukemia virus reverse transcriptase Invitrogen 28025013 3-isobutyl 1-methylxantine Sigma 28822-58-4 cAMP EIA kit Cayman Chemical 581001 Folskolin Sigma 66575-29-9 Crystal violet Wako 031-04852 Alexa Fluor 488 Invitrogen A11008 Goat anti-rabbit IgG antibody (H+ L), peroxidase Vector Laboratories PI-1000 Diaminobenzidine DOJINDO 349-00903 Sodium citrate buffer KANTO KAGAKU 73114 Carnoy’s solution Wako 034-17711 Alcian blue-PAS stain kit Scy Tek APS-1 FastDNA SPIN kit MP Biomedicals 116560200- CF AMPure XP Beckman Coulter A63881 RNeasy mini kit Qiagen 74904 RNA 6000 Nano kit Agilent Technologies 5067-1511 NEBNext® UltraTM II Directional RNA Library Prep Kit Illumina E7760S NEBNext Multiplex Oligos for Illumina Illumina E7335S IntestiCult organoid growth medium Veritas ST-06010 Software CRISPOR http://crispor.tefor.net trimmomatic (v0.39) https://github.com/usadellab/ Trimmomatic/releases/ download/v0.39/Trimmomatic0.39.zip FastQC (v0.11.8.-2) https://sourceforge.net/ projects/fastqc.mirror/files/ v0.11.8/ Reagent/resource Reference or source Identifier or catalog number STAR (v2.7.10a) https://github.com/alexdobin/ STAR/releases/download/ 2.7.11b/STAR_2.7.11b.zip GraphPad Prism https://www.graphpad.com/ Primer 3 https://primer3.ut.ee/ Other Millicell-ERS system Millipore MERS00002 StepOne real-time PCR system Applied Biosystems SYBR Premix Ex Taq II TAKARA RR820A Preparation of crosssections Kyoto Institute of Nutrition & Pathology, Inc GCMS-QP2010 Ultra system Shimazu MiSeq platform Illumina Agilent 2100 Bioanalyzer Agilent Technologies NovaSeq 6000 platform Illumina Kyoto Encyclopedia of Genes and Genomes (KEGG) database http://www.genome.jp/kegg/ Fluorescence microscope Keyence BZ-X710 .. Male C57BL/6J mice were purchased from Japan SLC and Or51e1 gene knockout (Gpr164−/−) mice were generated by using the CRISPR/Cas9 system in wild-type C57BL/6J zygotes (Fig. EV1D–F).



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