Incubation:Article Title: Characterising commensal and pathogenic staphylococcal interactions with neonatal and adult blood
Article Snippet: Cells were lysed by 3 × 1-min cycles of bead-beating at 3,000 rpm on a BioSpec Mini-Beadbeater-24 with 1 min breaks on ice. .. Samples were incubated at RT for 5 min before phase separation was performed by addition of 200 μl chloroform (MP Biomedical), followed by a 2 min incubation at RT and centrifugation at 12,000 x g for 15 min. RNA-containing aqueous phase was purified using the RNA Clean-and-Concentrator-25 kit (Zymo Research) according to the manufacturer’s instructions with a final elution volume of 30 μl. .. 40 U of RNaseOUTTM Recombinant Ribonuclease Inhibitor (40 U/μl, InvitrogenTM) were added to eluted RNA samples before any remaining DNA traces were removed using the Ambion ® DNA-freeTM DNase Treatment and Removal Reagents (Life Technologies) as per the manufacturer’s protocol.
Article Title: Characterising commensal and pathogenic staphylococcal interactions with neonatal and adult blood.
Article Snippet: Cells were lysed by 3x 1-min cycles of bead-beating at 3,000 rpm on a BioSpec 624 Mini-Beadbeater-24 with 1 min breaks on ice. .. Samples were incubated at RT for 5 min before 625 phase separation was performed by addition of 200 μl chloroform (MP Biomedical), followed 626 by a 2 min incubation at RT and centrifugation at 12,000 x g for 15 min. RNA-containing 627 aqueous phase was purified using the RNA Clean-and-Concentrator-25 kit (Zymo Research) 628 according to the manufacturer’s instructions with a final elution volume of 30 μl. .. 40 U of 629 RNaseOUTTM Recombinant Ribonuclease Inhibitor (40 U/μl, InvitrogenTM) were added to 630 eluted RNA samples before any remaining DNA traces were removed using the Ambion® 631 DNA-freeTM DNase Treatment and Removal Reagents (Life Technologies) as per the 632 manufacturer’s protocol.
Centrifugation:Article Title: Characterising commensal and pathogenic staphylococcal interactions with neonatal and adult blood
Article Snippet: Cells were lysed by 3 × 1-min cycles of bead-beating at 3,000 rpm on a BioSpec Mini-Beadbeater-24 with 1 min breaks on ice. .. Samples were incubated at RT for 5 min before phase separation was performed by addition of 200 μl chloroform (MP Biomedical), followed by a 2 min incubation at RT and centrifugation at 12,000 x g for 15 min. RNA-containing aqueous phase was purified using the RNA Clean-and-Concentrator-25 kit (Zymo Research) according to the manufacturer’s instructions with a final elution volume of 30 μl. .. 40 U of RNaseOUTTM Recombinant Ribonuclease Inhibitor (40 U/μl, InvitrogenTM) were added to eluted RNA samples before any remaining DNA traces were removed using the Ambion ® DNA-freeTM DNase Treatment and Removal Reagents (Life Technologies) as per the manufacturer’s protocol.
Article Title: Characterising commensal and pathogenic staphylococcal interactions with neonatal and adult blood.
Article Snippet: Cells were lysed by 3x 1-min cycles of bead-beating at 3,000 rpm on a BioSpec 624 Mini-Beadbeater-24 with 1 min breaks on ice. .. Samples were incubated at RT for 5 min before 625 phase separation was performed by addition of 200 μl chloroform (MP Biomedical), followed 626 by a 2 min incubation at RT and centrifugation at 12,000 x g for 15 min. RNA-containing 627 aqueous phase was purified using the RNA Clean-and-Concentrator-25 kit (Zymo Research) 628 according to the manufacturer’s instructions with a final elution volume of 30 μl. .. 40 U of 629 RNaseOUTTM Recombinant Ribonuclease Inhibitor (40 U/μl, InvitrogenTM) were added to 630 eluted RNA samples before any remaining DNA traces were removed using the Ambion® 631 DNA-freeTM DNase Treatment and Removal Reagents (Life Technologies) as per the 632 manufacturer’s protocol.
Purification:Article Title: Characterising commensal and pathogenic staphylococcal interactions with neonatal and adult blood
Article Snippet: Cells were lysed by 3 × 1-min cycles of bead-beating at 3,000 rpm on a BioSpec Mini-Beadbeater-24 with 1 min breaks on ice. .. Samples were incubated at RT for 5 min before phase separation was performed by addition of 200 μl chloroform (MP Biomedical), followed by a 2 min incubation at RT and centrifugation at 12,000 x g for 15 min. RNA-containing aqueous phase was purified using the RNA Clean-and-Concentrator-25 kit (Zymo Research) according to the manufacturer’s instructions with a final elution volume of 30 μl. .. 40 U of RNaseOUTTM Recombinant Ribonuclease Inhibitor (40 U/μl, InvitrogenTM) were added to eluted RNA samples before any remaining DNA traces were removed using the Ambion ® DNA-freeTM DNase Treatment and Removal Reagents (Life Technologies) as per the manufacturer’s protocol.
Article Title: Fungal community development in the soils of a mixed temperate forest reflects harvesting intensity following gap felling and selective cutting
Article Snippet: Forestry is crucial to the global economy, but its impact on ecosystems—especially under rising CO2 and climate change—prompts investigation of different tree harvesting methods to ensure long-term forest ecosystem sustainability and preserve its biodiversity.. We compared the effects of gap felling and selective cutting in a BeforeAfter-Control-Impact design of 18 patches of 30 m x 30 m on fungal community development over 3 years after cutting implementation in a tree species-rich temperate forest containing both ectomycorrhizal (EcM) and arbuscular mycorrhizal (AM) tree species.. Fungal biomass and community composition were monitored before tree harvesting and then over three consecutive years.
Article Title: Characterising commensal and pathogenic staphylococcal interactions with neonatal and adult blood.
Article Snippet: Cells were lysed by 3x 1-min cycles of bead-beating at 3,000 rpm on a BioSpec 624 Mini-Beadbeater-24 with 1 min breaks on ice. .. Samples were incubated at RT for 5 min before 625 phase separation was performed by addition of 200 μl chloroform (MP Biomedical), followed 626 by a 2 min incubation at RT and centrifugation at 12,000 x g for 15 min. RNA-containing 627 aqueous phase was purified using the RNA Clean-and-Concentrator-25 kit (Zymo Research) 628 according to the manufacturer’s instructions with a final elution volume of 30 μl. .. 40 U of 629 RNaseOUTTM Recombinant Ribonuclease Inhibitor (40 U/μl, InvitrogenTM) were added to 630 eluted RNA samples before any remaining DNA traces were removed using the Ambion® 631 DNA-freeTM DNase Treatment and Removal Reagents (Life Technologies) as per the 632 manufacturer’s protocol.
Lysis:Article Title: A rapid ionic liquid-based DNA extraction method for molecular diagnostics of urinary tract infections.
Article Snippet: .. In short, the pellet was resuspended in 0.9% NaCl, and cell lysis was achieved by adding CTAB buffer, phenol, chloroform/isoamyl alcohol, and glass beads in a FastPrep 24 bench-top homogenizer (MP Biomedicals Inc., USA) at a speed setting of 6 m/s for 30 s. DNA was precipitated with isopropanol and washed with ethanol. ..
Article Title: A rapid ionic liquid-based DNA extraction method for molecular diagnostics of urinary tract infections
Article Snippet: .. In short, the pellet was resuspended in 0.9% NaCl, and cell lysis was achieved by adding CTAB buffer, phenol, chloroform/isoamyl alcohol, and glass beads in a FastPrep 24 bench-top homogenizer (MP Biomedicals Inc., USA) at a speed setting of 6 m/s for 30 s. DNA was precipitated with isopropanol and washed with ethanol. ..
Recombinant:Article Title: Rediscovery of the greater chestnut weevil highlights the power of digital platforms in biodiversity research and conservation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Curculio taxon information This paper Data S1D Chemicals, peptides, and recombinant proteins Cetyltrimethylammonium bromide (CTAB) Sigma Aldrich SKU 6269-100G Cetyltrimethylammonium bromide (CTAB) Extraction Solution G-Biosciences Cat #786-564 Sodium chloride (NaCl) Fisher Scientific Cat #S271-1 Ethylenediaminetetraacetic acid (EDTA) Fisher Scientific Cat #BP120-500 tris(hydroxymethyl)aminomethane Thermo Scientific Cat #17926 Hydrochloric acid Fisher Scientific Cat #A144C-212 β-mercaptoethanol G-Biosciences Cat #P001 High-performance liquid chromatography (HPLC)-grade water Fisher Scientific Cat #W5-1 Proteinase K G-Biosciences Cat #786-038 RNase A Thermo Scientific Cat #EN0531 Chloroform Fisher Scientific Cat #C606-4 Isoamyl alcohol MP Biomedicals SKU 0215507880 Isopropyl alcohol Fisher Scientific Cat #A451-4 Ethanol – 200 Proof Decon Laboratories Cat #04-355-452 Critical commercial assays NEBNext Ultra II DNA Library Prep Kit for Illumina New England Biolabs NEB #E7645L NEBNext Multiplex Oligos for Illumina New England Biolabs NEB #E76002 NEBNext Multiplex Oligos for Illumina New England Biolabs NEB #6440S NEBNext Multiplex Oligos for Illumina New England Biolabs NEB #E6442S myBaits Target Capture Kit Daicel Arbor Biosciences Cat #300048.V5 Deposited data Raw Reads (Ingroup Taxa) This paper NCBI BioProject: PRJNA1190358 Raw Reads (Outgroup Taxa) Haran et al. 38 NCBI SRA: SRS19152263 and SRA: SRS19152262 C. caryatrypes Occurrence Data This paper Data S1B Curculio taxon information This paper Data S1D iNaturalist Observation Data This paper Zenodo: https://doi.org/10.5281/zenodo.14207480 Bird Data This paper Zenodo: https://doi.org/10.5281/zenodo.14207480 Chestnut Count Data This paper Zenodo: https://doi.org/10.5281/zenodo.14207480 Coleoptera AHE Probe Sequences Haddad et al. 45 Dryad: https://doi.org/10.5061/dryad.v0b7v Software and algorithms Phylogenomic Pipeline and Scripts Haran et al. 38 Zenodo: https://doi.org/10.5281/zenodo.7849369 IQ-TREE v.2.2.2.7 Nguyen et al. 46 https://github.com/iqtree/iqtree2/releases; RRID: SCR_017254 Trim Galore v.0.6.6 Babraham Bioinformatics https://github.com/FelixKrueger/TrimGalore; RRID: SCR_011847 NCBI Blastn Camacho et al. 47 https://blast.ncbi.nlm.nih.gov/Blast.cgi; RRID: SCR_001598 USEARCH Edgar 48 https://www.drive5.com/usearch/; RRID: SCR_027438 MAFFT v.7.407 Katoh et al. 43 https://mafft.cbrc.jp/alignment/software/; RRID: SCR_011811 (Continued on next page) Current Biology 35, 1–8.e1–e3, December 15, 2025 e1 Report ..
Extraction:Article Title: Rediscovery of the greater chestnut weevil highlights the power of digital platforms in biodiversity research and conservation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Curculio taxon information This paper Data S1D Chemicals, peptides, and recombinant proteins Cetyltrimethylammonium bromide (CTAB) Sigma Aldrich SKU 6269-100G Cetyltrimethylammonium bromide (CTAB) Extraction Solution G-Biosciences Cat #786-564 Sodium chloride (NaCl) Fisher Scientific Cat #S271-1 Ethylenediaminetetraacetic acid (EDTA) Fisher Scientific Cat #BP120-500 tris(hydroxymethyl)aminomethane Thermo Scientific Cat #17926 Hydrochloric acid Fisher Scientific Cat #A144C-212 β-mercaptoethanol G-Biosciences Cat #P001 High-performance liquid chromatography (HPLC)-grade water Fisher Scientific Cat #W5-1 Proteinase K G-Biosciences Cat #786-038 RNase A Thermo Scientific Cat #EN0531 Chloroform Fisher Scientific Cat #C606-4 Isoamyl alcohol MP Biomedicals SKU 0215507880 Isopropyl alcohol Fisher Scientific Cat #A451-4 Ethanol – 200 Proof Decon Laboratories Cat #04-355-452 Critical commercial assays NEBNext Ultra II DNA Library Prep Kit for Illumina New England Biolabs NEB #E7645L NEBNext Multiplex Oligos for Illumina New England Biolabs NEB #E76002 NEBNext Multiplex Oligos for Illumina New England Biolabs NEB #6440S NEBNext Multiplex Oligos for Illumina New England Biolabs NEB #E6442S myBaits Target Capture Kit Daicel Arbor Biosciences Cat #300048.V5 Deposited data Raw Reads (Ingroup Taxa) This paper NCBI BioProject: PRJNA1190358 Raw Reads (Outgroup Taxa) Haran et al. 38 NCBI SRA: SRS19152263 and SRA: SRS19152262 C. caryatrypes Occurrence Data This paper Data S1B Curculio taxon information This paper Data S1D iNaturalist Observation Data This paper Zenodo: https://doi.org/10.5281/zenodo.14207480 Bird Data This paper Zenodo: https://doi.org/10.5281/zenodo.14207480 Chestnut Count Data This paper Zenodo: https://doi.org/10.5281/zenodo.14207480 Coleoptera AHE Probe Sequences Haddad et al. 45 Dryad: https://doi.org/10.5061/dryad.v0b7v Software and algorithms Phylogenomic Pipeline and Scripts Haran et al. 38 Zenodo: https://doi.org/10.5281/zenodo.7849369 IQ-TREE v.2.2.2.7 Nguyen et al. 46 https://github.com/iqtree/iqtree2/releases; RRID: SCR_017254 Trim Galore v.0.6.6 Babraham Bioinformatics https://github.com/FelixKrueger/TrimGalore; RRID: SCR_011847 NCBI Blastn Camacho et al. 47 https://blast.ncbi.nlm.nih.gov/Blast.cgi; RRID: SCR_001598 USEARCH Edgar 48 https://www.drive5.com/usearch/; RRID: SCR_027438 MAFFT v.7.407 Katoh et al. 43 https://mafft.cbrc.jp/alignment/software/; RRID: SCR_011811 (Continued on next page) Current Biology 35, 1–8.e1–e3, December 15, 2025 e1 Report ..
Article Title: Fungal community development in the soils of a mixed temperate forest reflects harvesting intensity following gap felling and selective cutting
Article Snippet: Forestry is crucial to the global economy, but its impact on ecosystems—especially under rising CO2 and climate change—prompts investigation of different tree harvesting methods to ensure long-term forest ecosystem sustainability and preserve its biodiversity.. We compared the effects of gap felling and selective cutting in a BeforeAfter-Control-Impact design of 18 patches of 30 m x 30 m on fungal community development over 3 years after cutting implementation in a tree species-rich temperate forest containing both ectomycorrhizal (EcM) and arbuscular mycorrhizal (AM) tree species.. Fungal biomass and community composition were monitored before tree harvesting and then over three consecutive years.
High Performance Liquid Chromatography:Article Title: Rediscovery of the greater chestnut weevil highlights the power of digital platforms in biodiversity research and conservation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Curculio taxon information This paper Data S1D Chemicals, peptides, and recombinant proteins Cetyltrimethylammonium bromide (CTAB) Sigma Aldrich SKU 6269-100G Cetyltrimethylammonium bromide (CTAB) Extraction Solution G-Biosciences Cat #786-564 Sodium chloride (NaCl) Fisher Scientific Cat #S271-1 Ethylenediaminetetraacetic acid (EDTA) Fisher Scientific Cat #BP120-500 tris(hydroxymethyl)aminomethane Thermo Scientific Cat #17926 Hydrochloric acid Fisher Scientific Cat #A144C-212 β-mercaptoethanol G-Biosciences Cat #P001 High-performance liquid chromatography (HPLC)-grade water Fisher Scientific Cat #W5-1 Proteinase K G-Biosciences Cat #786-038 RNase A Thermo Scientific Cat #EN0531 Chloroform Fisher Scientific Cat #C606-4 Isoamyl alcohol MP Biomedicals SKU 0215507880 Isopropyl alcohol Fisher Scientific Cat #A451-4 Ethanol – 200 Proof Decon Laboratories Cat #04-355-452 Critical commercial assays NEBNext Ultra II DNA Library Prep Kit for Illumina New England Biolabs NEB #E7645L NEBNext Multiplex Oligos for Illumina New England Biolabs NEB #E76002 NEBNext Multiplex Oligos for Illumina New England Biolabs NEB #6440S NEBNext Multiplex Oligos for Illumina New England Biolabs NEB #E6442S myBaits Target Capture Kit Daicel Arbor Biosciences Cat #300048.V5 Deposited data Raw Reads (Ingroup Taxa) This paper NCBI BioProject: PRJNA1190358 Raw Reads (Outgroup Taxa) Haran et al. 38 NCBI SRA: SRS19152263 and SRA: SRS19152262 C. caryatrypes Occurrence Data This paper Data S1B Curculio taxon information This paper Data S1D iNaturalist Observation Data This paper Zenodo: https://doi.org/10.5281/zenodo.14207480 Bird Data This paper Zenodo: https://doi.org/10.5281/zenodo.14207480 Chestnut Count Data This paper Zenodo: https://doi.org/10.5281/zenodo.14207480 Coleoptera AHE Probe Sequences Haddad et al. 45 Dryad: https://doi.org/10.5061/dryad.v0b7v Software and algorithms Phylogenomic Pipeline and Scripts Haran et al. 38 Zenodo: https://doi.org/10.5281/zenodo.7849369 IQ-TREE v.2.2.2.7 Nguyen et al. 46 https://github.com/iqtree/iqtree2/releases; RRID: SCR_017254 Trim Galore v.0.6.6 Babraham Bioinformatics https://github.com/FelixKrueger/TrimGalore; RRID: SCR_011847 NCBI Blastn Camacho et al. 47 https://blast.ncbi.nlm.nih.gov/Blast.cgi; RRID: SCR_001598 USEARCH Edgar 48 https://www.drive5.com/usearch/; RRID: SCR_027438 MAFFT v.7.407 Katoh et al. 43 https://mafft.cbrc.jp/alignment/software/; RRID: SCR_011811 (Continued on next page) Current Biology 35, 1–8.e1–e3, December 15, 2025 e1 Report ..
Multiplex Assay:Article Title: Rediscovery of the greater chestnut weevil highlights the power of digital platforms in biodiversity research and conservation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Curculio taxon information This paper Data S1D Chemicals, peptides, and recombinant proteins Cetyltrimethylammonium bromide (CTAB) Sigma Aldrich SKU 6269-100G Cetyltrimethylammonium bromide (CTAB) Extraction Solution G-Biosciences Cat #786-564 Sodium chloride (NaCl) Fisher Scientific Cat #S271-1 Ethylenediaminetetraacetic acid (EDTA) Fisher Scientific Cat #BP120-500 tris(hydroxymethyl)aminomethane Thermo Scientific Cat #17926 Hydrochloric acid Fisher Scientific Cat #A144C-212 β-mercaptoethanol G-Biosciences Cat #P001 High-performance liquid chromatography (HPLC)-grade water Fisher Scientific Cat #W5-1 Proteinase K G-Biosciences Cat #786-038 RNase A Thermo Scientific Cat #EN0531 Chloroform Fisher Scientific Cat #C606-4 Isoamyl alcohol MP Biomedicals SKU 0215507880 Isopropyl alcohol Fisher Scientific Cat #A451-4 Ethanol – 200 Proof Decon Laboratories Cat #04-355-452 Critical commercial assays NEBNext Ultra II DNA Library Prep Kit for Illumina New England Biolabs NEB #E7645L NEBNext Multiplex Oligos for Illumina New England Biolabs NEB #E76002 NEBNext Multiplex Oligos for Illumina New England Biolabs NEB #6440S NEBNext Multiplex Oligos for Illumina New England Biolabs NEB #E6442S myBaits Target Capture Kit Daicel Arbor Biosciences Cat #300048.V5 Deposited data Raw Reads (Ingroup Taxa) This paper NCBI BioProject: PRJNA1190358 Raw Reads (Outgroup Taxa) Haran et al. 38 NCBI SRA: SRS19152263 and SRA: SRS19152262 C. caryatrypes Occurrence Data This paper Data S1B Curculio taxon information This paper Data S1D iNaturalist Observation Data This paper Zenodo: https://doi.org/10.5281/zenodo.14207480 Bird Data This paper Zenodo: https://doi.org/10.5281/zenodo.14207480 Chestnut Count Data This paper Zenodo: https://doi.org/10.5281/zenodo.14207480 Coleoptera AHE Probe Sequences Haddad et al. 45 Dryad: https://doi.org/10.5061/dryad.v0b7v Software and algorithms Phylogenomic Pipeline and Scripts Haran et al. 38 Zenodo: https://doi.org/10.5281/zenodo.7849369 IQ-TREE v.2.2.2.7 Nguyen et al. 46 https://github.com/iqtree/iqtree2/releases; RRID: SCR_017254 Trim Galore v.0.6.6 Babraham Bioinformatics https://github.com/FelixKrueger/TrimGalore; RRID: SCR_011847 NCBI Blastn Camacho et al. 47 https://blast.ncbi.nlm.nih.gov/Blast.cgi; RRID: SCR_001598 USEARCH Edgar 48 https://www.drive5.com/usearch/; RRID: SCR_027438 MAFFT v.7.407 Katoh et al. 43 https://mafft.cbrc.jp/alignment/software/; RRID: SCR_011811 (Continued on next page) Current Biology 35, 1–8.e1–e3, December 15, 2025 e1 Report ..
Software:Article Title: Rediscovery of the greater chestnut weevil highlights the power of digital platforms in biodiversity research and conservation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Curculio taxon information This paper Data S1D Chemicals, peptides, and recombinant proteins Cetyltrimethylammonium bromide (CTAB) Sigma Aldrich SKU 6269-100G Cetyltrimethylammonium bromide (CTAB) Extraction Solution G-Biosciences Cat #786-564 Sodium chloride (NaCl) Fisher Scientific Cat #S271-1 Ethylenediaminetetraacetic acid (EDTA) Fisher Scientific Cat #BP120-500 tris(hydroxymethyl)aminomethane Thermo Scientific Cat #17926 Hydrochloric acid Fisher Scientific Cat #A144C-212 β-mercaptoethanol G-Biosciences Cat #P001 High-performance liquid chromatography (HPLC)-grade water Fisher Scientific Cat #W5-1 Proteinase K G-Biosciences Cat #786-038 RNase A Thermo Scientific Cat #EN0531 Chloroform Fisher Scientific Cat #C606-4 Isoamyl alcohol MP Biomedicals SKU 0215507880 Isopropyl alcohol Fisher Scientific Cat #A451-4 Ethanol – 200 Proof Decon Laboratories Cat #04-355-452 Critical commercial assays NEBNext Ultra II DNA Library Prep Kit for Illumina New England Biolabs NEB #E7645L NEBNext Multiplex Oligos for Illumina New England Biolabs NEB #E76002 NEBNext Multiplex Oligos for Illumina New England Biolabs NEB #6440S NEBNext Multiplex Oligos for Illumina New England Biolabs NEB #E6442S myBaits Target Capture Kit Daicel Arbor Biosciences Cat #300048.V5 Deposited data Raw Reads (Ingroup Taxa) This paper NCBI BioProject: PRJNA1190358 Raw Reads (Outgroup Taxa) Haran et al. 38 NCBI SRA: SRS19152263 and SRA: SRS19152262 C. caryatrypes Occurrence Data This paper Data S1B Curculio taxon information This paper Data S1D iNaturalist Observation Data This paper Zenodo: https://doi.org/10.5281/zenodo.14207480 Bird Data This paper Zenodo: https://doi.org/10.5281/zenodo.14207480 Chestnut Count Data This paper Zenodo: https://doi.org/10.5281/zenodo.14207480 Coleoptera AHE Probe Sequences Haddad et al. 45 Dryad: https://doi.org/10.5061/dryad.v0b7v Software and algorithms Phylogenomic Pipeline and Scripts Haran et al. 38 Zenodo: https://doi.org/10.5281/zenodo.7849369 IQ-TREE v.2.2.2.7 Nguyen et al. 46 https://github.com/iqtree/iqtree2/releases; RRID: SCR_017254 Trim Galore v.0.6.6 Babraham Bioinformatics https://github.com/FelixKrueger/TrimGalore; RRID: SCR_011847 NCBI Blastn Camacho et al. 47 https://blast.ncbi.nlm.nih.gov/Blast.cgi; RRID: SCR_001598 USEARCH Edgar 48 https://www.drive5.com/usearch/; RRID: SCR_027438 MAFFT v.7.407 Katoh et al. 43 https://mafft.cbrc.jp/alignment/software/; RRID: SCR_011811 (Continued on next page) Current Biology 35, 1–8.e1–e3, December 15, 2025 e1 Report ..
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