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Valiant Co Ltd ethidium bromide
Ethidium Bromide, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 179 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/02193993%2E2/Ethidium+bromide/10__1134_slash_s1068162024050327-122-60-77
Average 94 stars, based on 179 article reviews
ethidium bromide - by Bioz Stars, 2026-09
94/100 stars

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Related Articles

Staining:

Article Title: Alternative splicing of the Snap23 microexon is regulated by MBNL, QKI, and RBFOX2 in a tissue-specific manner and is altered in striated muscle diseases
Article Snippet: PCR products were loaded alongside the pUC19 ladder (Thermo Fisher Scientific, SM0221) into a 6% polyacrylamide gel in TAE buffer (40 mm Tris, 20 mm acetic acid, 1 mm EDTA (pH 8.0)), and the bands were resolved at 140–150 V for 2.5–3 hours. .. The DNA was stained by submerging the gel into an aqueous solution of 0.4 μg/mL ethidium bromide (MP Biomedicals, ETBC1001) for 10 minutes, and the gel was imaged on the ChemiDoc XRS+ Imaging System (Bio-Rad). .. Alternative splicing was quantified by densitometry using the Image Lab software (Bio-Rad), and the percent spliced in (PSI) [ ] was calculated.

Article Title: Snord67 promotes breast cancer metastasis by guiding U6 modification and modulating the splicing landscape
Article Snippet: The pUC19 ladder (Thermo Scientific, SM0221) was loaded alongside samples. .. The gel was submerged in an aqueous solution of 0.4 μg/mL ethidium bromide (MP Biomedicals, ETBC1001) for 10 minutes to stain the DNA. .. The ChemiDoc XRS+ Imaging System (Bio-Rad) was used to visualize bands, and the Image Lab software (Bio-Rad) was used to quantify the bands by densitometry.

Article Title: Snord67 promotes breast cancer metastasis by guiding U6 modification and modulating the splicing landscape.
Article Snippet: The pUC19 ladder (Thermo Scientific, SM0221) was loaded alongside samples. .. The gel was submerged in an aqueous solution of 0.4 μg/mL ethidium bromide (MP Biomedicals, ETBC1001) for 10minutes to stain the DNA. .. The ChemiDoc XRS+ Imaging System (Bio-Rad) was used to visualize bands, and the Image Lab software (Bio-Rad) was used to quantify the bands by densitometry.

Article Title: Interleukin-10 limits immune-mediated pathology in chronic subclinical plasmodial infection.
Article Snippet: .. The size of the PCR product was predicted as 274 bp and confirmed in a 2% agarose gel in 1x Tris Acetate-EDTA (TAE) buffer (Sigma, T9650), stained by ethidium bromide (MP Biomedicals, LLC – Cat. 802511), and a 100 bp DNA ladder (Thermo Scientific SM0243) was used as a reference. .. An agarose gel loading dye 6X, glycerol-based (Boston Bioproducts – Cat. BM-100G), was added to samples, which ran for 45 minutes at 124V in an Eppendorf thermocycler Mastercycler nexus GX2, and the images were acquired in a ChemiDoc Touch Imaging System (Bio-Rad).

Article Title: Interleukin-10 limits immune-mediated pathology in chronic subclinical plasmodial infection
Article Snippet: .. The size of the PCR product was predicted as 274 bp and confirmed in a 2% agarose gel in 1x Tris Acetate-EDTA (TAE) buffer (Sigma, T9650), stained by ethidium bromide (MP Biomedicals, LLC – Cat. 802511), and a 100 bp DNA ladder (Thermo Scientific SM0243) was used as a reference. .. An agarose gel loading dye 6X, glycerol-based (Boston Bioproducts – Cat. BM-100G), was added to samples, which ran for 45 minutes at 124V in an Eppendorf thermocycler Mastercycler nexus GX2, and the images were acquired in a ChemiDoc Touch Imaging System (Bio-Rad).

Imaging:

Article Title: Alternative splicing of the Snap23 microexon is regulated by MBNL, QKI, and RBFOX2 in a tissue-specific manner and is altered in striated muscle diseases
Article Snippet: PCR products were loaded alongside the pUC19 ladder (Thermo Fisher Scientific, SM0221) into a 6% polyacrylamide gel in TAE buffer (40 mm Tris, 20 mm acetic acid, 1 mm EDTA (pH 8.0)), and the bands were resolved at 140–150 V for 2.5–3 hours. .. The DNA was stained by submerging the gel into an aqueous solution of 0.4 μg/mL ethidium bromide (MP Biomedicals, ETBC1001) for 10 minutes, and the gel was imaged on the ChemiDoc XRS+ Imaging System (Bio-Rad). .. Alternative splicing was quantified by densitometry using the Image Lab software (Bio-Rad), and the percent spliced in (PSI) [ ] was calculated.

Polymerase Chain Reaction:

Article Title: Interleukin-10 limits immune-mediated pathology in chronic subclinical plasmodial infection.
Article Snippet: .. The size of the PCR product was predicted as 274 bp and confirmed in a 2% agarose gel in 1x Tris Acetate-EDTA (TAE) buffer (Sigma, T9650), stained by ethidium bromide (MP Biomedicals, LLC – Cat. 802511), and a 100 bp DNA ladder (Thermo Scientific SM0243) was used as a reference. .. An agarose gel loading dye 6X, glycerol-based (Boston Bioproducts – Cat. BM-100G), was added to samples, which ran for 45 minutes at 124V in an Eppendorf thermocycler Mastercycler nexus GX2, and the images were acquired in a ChemiDoc Touch Imaging System (Bio-Rad).

Article Title: Interleukin-10 limits immune-mediated pathology in chronic subclinical plasmodial infection
Article Snippet: .. The size of the PCR product was predicted as 274 bp and confirmed in a 2% agarose gel in 1x Tris Acetate-EDTA (TAE) buffer (Sigma, T9650), stained by ethidium bromide (MP Biomedicals, LLC – Cat. 802511), and a 100 bp DNA ladder (Thermo Scientific SM0243) was used as a reference. .. An agarose gel loading dye 6X, glycerol-based (Boston Bioproducts – Cat. BM-100G), was added to samples, which ran for 45 minutes at 124V in an Eppendorf thermocycler Mastercycler nexus GX2, and the images were acquired in a ChemiDoc Touch Imaging System (Bio-Rad).

Agarose Gel Electrophoresis:

Article Title: Interleukin-10 limits immune-mediated pathology in chronic subclinical plasmodial infection.
Article Snippet: .. The size of the PCR product was predicted as 274 bp and confirmed in a 2% agarose gel in 1x Tris Acetate-EDTA (TAE) buffer (Sigma, T9650), stained by ethidium bromide (MP Biomedicals, LLC – Cat. 802511), and a 100 bp DNA ladder (Thermo Scientific SM0243) was used as a reference. .. An agarose gel loading dye 6X, glycerol-based (Boston Bioproducts – Cat. BM-100G), was added to samples, which ran for 45 minutes at 124V in an Eppendorf thermocycler Mastercycler nexus GX2, and the images were acquired in a ChemiDoc Touch Imaging System (Bio-Rad).

Article Title: Interleukin-10 limits immune-mediated pathology in chronic subclinical plasmodial infection
Article Snippet: .. The size of the PCR product was predicted as 274 bp and confirmed in a 2% agarose gel in 1x Tris Acetate-EDTA (TAE) buffer (Sigma, T9650), stained by ethidium bromide (MP Biomedicals, LLC – Cat. 802511), and a 100 bp DNA ladder (Thermo Scientific SM0243) was used as a reference. .. An agarose gel loading dye 6X, glycerol-based (Boston Bioproducts – Cat. BM-100G), was added to samples, which ran for 45 minutes at 124V in an Eppendorf thermocycler Mastercycler nexus GX2, and the images were acquired in a ChemiDoc Touch Imaging System (Bio-Rad).

Modification:

Article Title: Reno-protection by Didymocarpus pedicellata against oxalate-induced damage: mechanistic insights into mitigation of oxidative stress induced signaling pathways in renal cell line and rat model.
Article Snippet: 1 School of Biotechnology, Gautam Buddha University, Greater Noida, Uttar Pradesh, India 2 Amity Institute of Molecular Medicine and Stem Cell Research (AIMMSCR), Amity University Uttar Pradesh, Noida, India 3 Department of Biophysics, Panjab University, Chandigarh, India 4 Amity University Uttar Pradesh, Noida, India Abstract Oxidative stress is a key driver of inflammatory injury which predisposes renal cells to nephrolithiatic damage.. Recurrent stone formation is associated with cellular dysfunction, oxidative stress, inflammation and renal epithelial cell death.. Didymocarpus pedicellata is an important medicinal herb in the ayurvedic system for the treatment of renal afflictions.

Saline:

Article Title: Reno-protection by Didymocarpus pedicellata against oxalate-induced damage: mechanistic insights into mitigation of oxidative stress induced signaling pathways in renal cell line and rat model.
Article Snippet: 1 School of Biotechnology, Gautam Buddha University, Greater Noida, Uttar Pradesh, India 2 Amity Institute of Molecular Medicine and Stem Cell Research (AIMMSCR), Amity University Uttar Pradesh, Noida, India 3 Department of Biophysics, Panjab University, Chandigarh, India 4 Amity University Uttar Pradesh, Noida, India Abstract Oxidative stress is a key driver of inflammatory injury which predisposes renal cells to nephrolithiatic damage.. Recurrent stone formation is associated with cellular dysfunction, oxidative stress, inflammation and renal epithelial cell death.. Didymocarpus pedicellata is an important medicinal herb in the ayurvedic system for the treatment of renal afflictions.

cDNA Synthesis:

Article Title: Reno-protection by Didymocarpus pedicellata against oxalate-induced damage: mechanistic insights into mitigation of oxidative stress induced signaling pathways in renal cell line and rat model.
Article Snippet: 1 School of Biotechnology, Gautam Buddha University, Greater Noida, Uttar Pradesh, India 2 Amity Institute of Molecular Medicine and Stem Cell Research (AIMMSCR), Amity University Uttar Pradesh, Noida, India 3 Department of Biophysics, Panjab University, Chandigarh, India 4 Amity University Uttar Pradesh, Noida, India Abstract Oxidative stress is a key driver of inflammatory injury which predisposes renal cells to nephrolithiatic damage.. Recurrent stone formation is associated with cellular dysfunction, oxidative stress, inflammation and renal epithelial cell death.. Didymocarpus pedicellata is an important medicinal herb in the ayurvedic system for the treatment of renal afflictions.

Sterility:

Article Title: Protocol for efficient recovery of high-quality DNA from microbiome of marine invertebrates.
Article Snippet: .. - Sterile sea water - 50X TAE buffer (2 M Tris-acetate, 50 mM EDTA; pH 8.3) - 2 μM deoxynucleotide triphosphate (dNTPs) - 10X Ex-Taq buffer (200 mM Tris-HCl; pH 8.4, 500 mM KCl) - Ex-Taq DNA polymerase - 0.5 μg/μl ethidium bromide (EtBr) - Agarose - Absolute ethanol - TNE buffer (100 mM Tris-HCl, 100 mM NaCl, 0.5 mM EDTA; pH 8.0) - TE buffer (10 mM Tris-HCl, 1 mM EDTA; pH 8.0) - 10% sodium lauryl sarcosine - 10% SDS solution - 10 mg/ml RNase A - 20 mg/ml proteinase K - Phenol-chloroform-isoamylalcohol (25:24:1) solution - 3 M sodium acetate - DNase-free water - Purification buffers in FastDNATM SPIN Kit for Soil (Cat. # 6560220, MP Biomedicals): Binding Matrix suspension, SEWS-M wash buffer, DES elution buffer B. Equipments - Micro-centrifuge - Conical tube centrifuge - Vortex mixer - Heating block (or water bath) - Thermal cycler - Nanodrop Spectrophotometer ND-2000 (Thermo Fisher) - Handy held homogenizer (Possible to replace with kitchen blender) ..

Purification:

Article Title: Protocol for efficient recovery of high-quality DNA from microbiome of marine invertebrates.
Article Snippet: .. - Sterile sea water - 50X TAE buffer (2 M Tris-acetate, 50 mM EDTA; pH 8.3) - 2 μM deoxynucleotide triphosphate (dNTPs) - 10X Ex-Taq buffer (200 mM Tris-HCl; pH 8.4, 500 mM KCl) - Ex-Taq DNA polymerase - 0.5 μg/μl ethidium bromide (EtBr) - Agarose - Absolute ethanol - TNE buffer (100 mM Tris-HCl, 100 mM NaCl, 0.5 mM EDTA; pH 8.0) - TE buffer (10 mM Tris-HCl, 1 mM EDTA; pH 8.0) - 10% sodium lauryl sarcosine - 10% SDS solution - 10 mg/ml RNase A - 20 mg/ml proteinase K - Phenol-chloroform-isoamylalcohol (25:24:1) solution - 3 M sodium acetate - DNase-free water - Purification buffers in FastDNATM SPIN Kit for Soil (Cat. # 6560220, MP Biomedicals): Binding Matrix suspension, SEWS-M wash buffer, DES elution buffer B. Equipments - Micro-centrifuge - Conical tube centrifuge - Vortex mixer - Heating block (or water bath) - Thermal cycler - Nanodrop Spectrophotometer ND-2000 (Thermo Fisher) - Handy held homogenizer (Possible to replace with kitchen blender) ..

Binding Assay:

Article Title: Protocol for efficient recovery of high-quality DNA from microbiome of marine invertebrates.
Article Snippet: .. - Sterile sea water - 50X TAE buffer (2 M Tris-acetate, 50 mM EDTA; pH 8.3) - 2 μM deoxynucleotide triphosphate (dNTPs) - 10X Ex-Taq buffer (200 mM Tris-HCl; pH 8.4, 500 mM KCl) - Ex-Taq DNA polymerase - 0.5 μg/μl ethidium bromide (EtBr) - Agarose - Absolute ethanol - TNE buffer (100 mM Tris-HCl, 100 mM NaCl, 0.5 mM EDTA; pH 8.0) - TE buffer (10 mM Tris-HCl, 1 mM EDTA; pH 8.0) - 10% sodium lauryl sarcosine - 10% SDS solution - 10 mg/ml RNase A - 20 mg/ml proteinase K - Phenol-chloroform-isoamylalcohol (25:24:1) solution - 3 M sodium acetate - DNase-free water - Purification buffers in FastDNATM SPIN Kit for Soil (Cat. # 6560220, MP Biomedicals): Binding Matrix suspension, SEWS-M wash buffer, DES elution buffer B. Equipments - Micro-centrifuge - Conical tube centrifuge - Vortex mixer - Heating block (or water bath) - Thermal cycler - Nanodrop Spectrophotometer ND-2000 (Thermo Fisher) - Handy held homogenizer (Possible to replace with kitchen blender) ..

Suspension:

Article Title: Protocol for efficient recovery of high-quality DNA from microbiome of marine invertebrates.
Article Snippet: .. - Sterile sea water - 50X TAE buffer (2 M Tris-acetate, 50 mM EDTA; pH 8.3) - 2 μM deoxynucleotide triphosphate (dNTPs) - 10X Ex-Taq buffer (200 mM Tris-HCl; pH 8.4, 500 mM KCl) - Ex-Taq DNA polymerase - 0.5 μg/μl ethidium bromide (EtBr) - Agarose - Absolute ethanol - TNE buffer (100 mM Tris-HCl, 100 mM NaCl, 0.5 mM EDTA; pH 8.0) - TE buffer (10 mM Tris-HCl, 1 mM EDTA; pH 8.0) - 10% sodium lauryl sarcosine - 10% SDS solution - 10 mg/ml RNase A - 20 mg/ml proteinase K - Phenol-chloroform-isoamylalcohol (25:24:1) solution - 3 M sodium acetate - DNase-free water - Purification buffers in FastDNATM SPIN Kit for Soil (Cat. # 6560220, MP Biomedicals): Binding Matrix suspension, SEWS-M wash buffer, DES elution buffer B. Equipments - Micro-centrifuge - Conical tube centrifuge - Vortex mixer - Heating block (or water bath) - Thermal cycler - Nanodrop Spectrophotometer ND-2000 (Thermo Fisher) - Handy held homogenizer (Possible to replace with kitchen blender) ..

Blocking Assay:

Article Title: Protocol for efficient recovery of high-quality DNA from microbiome of marine invertebrates.
Article Snippet: .. - Sterile sea water - 50X TAE buffer (2 M Tris-acetate, 50 mM EDTA; pH 8.3) - 2 μM deoxynucleotide triphosphate (dNTPs) - 10X Ex-Taq buffer (200 mM Tris-HCl; pH 8.4, 500 mM KCl) - Ex-Taq DNA polymerase - 0.5 μg/μl ethidium bromide (EtBr) - Agarose - Absolute ethanol - TNE buffer (100 mM Tris-HCl, 100 mM NaCl, 0.5 mM EDTA; pH 8.0) - TE buffer (10 mM Tris-HCl, 1 mM EDTA; pH 8.0) - 10% sodium lauryl sarcosine - 10% SDS solution - 10 mg/ml RNase A - 20 mg/ml proteinase K - Phenol-chloroform-isoamylalcohol (25:24:1) solution - 3 M sodium acetate - DNase-free water - Purification buffers in FastDNATM SPIN Kit for Soil (Cat. # 6560220, MP Biomedicals): Binding Matrix suspension, SEWS-M wash buffer, DES elution buffer B. Equipments - Micro-centrifuge - Conical tube centrifuge - Vortex mixer - Heating block (or water bath) - Thermal cycler - Nanodrop Spectrophotometer ND-2000 (Thermo Fisher) - Handy held homogenizer (Possible to replace with kitchen blender) ..

Spectrophotometry:

Article Title: Protocol for efficient recovery of high-quality DNA from microbiome of marine invertebrates.
Article Snippet: .. - Sterile sea water - 50X TAE buffer (2 M Tris-acetate, 50 mM EDTA; pH 8.3) - 2 μM deoxynucleotide triphosphate (dNTPs) - 10X Ex-Taq buffer (200 mM Tris-HCl; pH 8.4, 500 mM KCl) - Ex-Taq DNA polymerase - 0.5 μg/μl ethidium bromide (EtBr) - Agarose - Absolute ethanol - TNE buffer (100 mM Tris-HCl, 100 mM NaCl, 0.5 mM EDTA; pH 8.0) - TE buffer (10 mM Tris-HCl, 1 mM EDTA; pH 8.0) - 10% sodium lauryl sarcosine - 10% SDS solution - 10 mg/ml RNase A - 20 mg/ml proteinase K - Phenol-chloroform-isoamylalcohol (25:24:1) solution - 3 M sodium acetate - DNase-free water - Purification buffers in FastDNATM SPIN Kit for Soil (Cat. # 6560220, MP Biomedicals): Binding Matrix suspension, SEWS-M wash buffer, DES elution buffer B. Equipments - Micro-centrifuge - Conical tube centrifuge - Vortex mixer - Heating block (or water bath) - Thermal cycler - Nanodrop Spectrophotometer ND-2000 (Thermo Fisher) - Handy held homogenizer (Possible to replace with kitchen blender) ..



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