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Valiant Co Ltd d l sulforaphane sfn

(A) Percent of NRF2 target genes overlapping human orthologs of Drosophila Class I, II, or III genes. Orthologs were identified using either the top scoring ortholog only (best ortholog), or all orthologs scoring >2 as described in the text. *P<0.0005, **P<0.001, #P<0.05, based on hypergeometric test. (B) Gene Set Enrichment Analysis (GSEA) comparing conserved human NRF2 target genes (human orthologs of Drosophila Class I genes) and gene expression changes after treatment of LCL cells with <t>sulforaphane</t> <t>(SFN).</t>

D L Sulforaphane Sfn, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/02193781-cf/DL-Sulforaphane/pmc04837897-160-0-5
Average 90 stars, based on 6 article reviews

d l sulforaphane sfn - by Bioz Stars, 2026-09

90/100 stars

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1) Product Images from "Beyond antioxidant genes in the ancient NRF2 regulatory network"

Article Title: Beyond antioxidant genes in the ancient NRF2 regulatory network

Journal: Free radical biology & medicine

doi: 10.1016/j.freeradbiomed.2015.06.044

Figure Legend Snippet: (A) Percent of NRF2 target genes overlapping human orthologs of Drosophila Class I, II, or III genes. Orthologs were identified using either the top scoring ortholog only (best ortholog), or all orthologs scoring >2 as described in the text. *P<0.0005, **P<0.001, #P<0.05, based on hypergeometric test. (B) Gene Set Enrichment Analysis (GSEA) comparing conserved human NRF2 target genes (human orthologs of Drosophila Class I genes) and gene expression changes after treatment of LCL cells with sulforaphane (SFN).

Techniques Used: Expressing

(A) Human NRF2 ChIP-seq signal from LCL cells treated with DMSO or sulforaphane (SFN) as indicated. Select ancient NRF2 target genes with highly significant binding are represented (ChIP y-axis scale = 0-5000). (B) ChIP-seq signal as in (A) at select ancient NRF2 target genes with moderate binding (ChIP y-axis scale = 0-500). (C) Heatmap representing the response to sulforaphane (SFN), tert-butylhydroquinone (tBHQ), overexpression of NRF2, or overexpression of a dominant negative version of NRF2 (NRF2DN) for reporter constructs driven by the enhancer regions highlighted in panels (A) and (B). NQO1 is a positive control for human NRF2, but is not a conserved target because insects do not have an orthologous gene; the remaining nine are enhancers at deeply conserved NRF2 target genes.

Figure Legend Snippet: (A) Human NRF2 ChIP-seq signal from LCL cells treated with DMSO or sulforaphane (SFN) as indicated. Select ancient NRF2 target genes with highly significant binding are represented (ChIP y-axis scale = 0-5000). (B) ChIP-seq signal as in (A) at select ancient NRF2 target genes with moderate binding (ChIP y-axis scale = 0-500). (C) Heatmap representing the response to sulforaphane (SFN), tert-butylhydroquinone (tBHQ), overexpression of NRF2, or overexpression of a dominant negative version of NRF2 (NRF2DN) for reporter constructs driven by the enhancer regions highlighted in panels (A) and (B). NQO1 is a positive control for human NRF2, but is not a conserved target because insects do not have an orthologous gene; the remaining nine are enhancers at deeply conserved NRF2 target genes.

Techniques Used: ChIP-sequencing, Binding Assay, Over Expression, Dominant Negative Mutation, Construct, Positive Control

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Article Title: Variation of glucoraphanin metabolism in vivo and ex vivo by human gut bacteria
Article Snippet: Samples were incubated either with or without 50 μM glucoraphanin (C 2 Bioengineering, Hovedgaden, Denmark) anaerobically in duplicate using a GasPak 150 System (Becton, Dickinson and Company, Franklin Lakes, NJ) for 24 or 48 h at 37°C. .. Briefly, 100 μl urine sample was incubated with 500 μl 100 mM K 2 HPO 4 (pH 8.5) and 600 μl benzene dithiol (1.42 g/l in 2-propanol, Sigma-Aldrich) in a shaking water bath at 65°C for 1 h. A set of sulforaphane (MP Biomedicals) calibration standards (5 – 500 μM), a blank and a quality control sample were treated in the same manner. .. We also added Trace Mineral Supplement and Vitamin Supplement (ATCC, Manassas, VA) into the medium to promote bacteria growth (1:100 v/v dilution).

Article Title: Variation of glucoraphanin metabolismin vivoandex vivoby human gut bacteria
Article Snippet: .. Briefly, 100ml of the urine sample were incubated with 500ml of 100 mM-K2HPO4 (pH 8·5) and 600ml benzenedithiol (1·42 g/l in 2-propanol; Sigma-Aldrich) in a shaking water-bath at 658C for 1 h. A set of sulforaphane (MP Biomedicals) calibration standards (5–500mM), a blank and a quality-control sample, were treated in the same manner. .. Chromatographic separation of the cyclocondensation product occurred under gradient conditions on a C18 mBondapak 150 £ 3·9 mm column (Waters Corporation, Milford, MA, USA), which was attached to an 1100 UV-HPLC (Agilent Technologies, Santa Clara, CA, USA).

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The ARE allele of rs667897 is responsive to NRF1 activation. (A) Allele-specific reporter assays in which the region encompassing rs667897 G (non-ARE allele) or rs667897 A (ARE allele) was cloned upstream of luciferase and transfected into HepG2 cells. Luciferase driven by the LDHA promoter was included as an internal control. Transfected cells were treated with vehicle (DMSO) control, sulforaphane, or MG132. (B) Quantitative, reverse transcription PCR monitoring of MG132-responsive MS4A6A expression in peripheral blood monocytes heterozygous (A/G) at rs667897. MS4A6A expression is significantly increased in MG132-treated cells. (C) Allele-specific HepG2 reporter assays, using the same rs667897 G (non-ARE allele) or rs667897 A (ARE allele) constructs from (A), only with knockdown of NRF1 ( NRF1 KD ) or NRF2 ( NRF2 KD ) using RNA interference. Cells were also treated with no RNAi (“none”) or scrambled RNAi duplexes (“Scram”) as negative controls. For all panels, values represent mean ± standard deviation, treatments were compared to the corresponding DMSO control, and asterisks represent an FDR-adjusted p-value < 0.05 (Welch's t -test).

Journal: Redox Biology

Article Title: A hypermorphic antioxidant response element is associated with increased MS4A6A expression and Alzheimer's disease

doi: 10.1016/j.redox.2017.10.018

Figure Lengend Snippet: The ARE allele of rs667897 is responsive to NRF1 activation. (A) Allele-specific reporter assays in which the region encompassing rs667897 G (non-ARE allele) or rs667897 A (ARE allele) was cloned upstream of luciferase and transfected into HepG2 cells. Luciferase driven by the LDHA promoter was included as an internal control. Transfected cells were treated with vehicle (DMSO) control, sulforaphane, or MG132. (B) Quantitative, reverse transcription PCR monitoring of MG132-responsive MS4A6A expression in peripheral blood monocytes heterozygous (A/G) at rs667897. MS4A6A expression is significantly increased in MG132-treated cells. (C) Allele-specific HepG2 reporter assays, using the same rs667897 G (non-ARE allele) or rs667897 A (ARE allele) constructs from (A), only with knockdown of NRF1 ( NRF1 KD ) or NRF2 ( NRF2 KD ) using RNA interference. Cells were also treated with no RNAi (“none”) or scrambled RNAi duplexes (“Scram”) as negative controls. For all panels, values represent mean ± standard deviation, treatments were compared to the corresponding DMSO control, and asterisks represent an FDR-adjusted p-value < 0.05 (Welch's t -test).

Article Snippet: D,L-sulforaphane (SFN) was purchased from MP Biomedicals LLC (Santa Ana, CA).

Techniques: Activation Assay, Clone Assay, Luciferase, Transfection, Expressing, Construct, Standard Deviation

Journal: Free radical biology & medicine

Article Title: Beyond antioxidant genes in the ancient NRF2 regulatory network

doi: 10.1016/j.freeradbiomed.2015.06.044

Figure Lengend Snippet: (A) Percent of NRF2 target genes overlapping human orthologs of Drosophila Class I, II, or III genes. Orthologs were identified using either the top scoring ortholog only (best ortholog), or all orthologs scoring >2 as described in the text. *P<0.0005, **P<0.001, #P<0.05, based on hypergeometric test. (B) Gene Set Enrichment Analysis (GSEA) comparing conserved human NRF2 target genes (human orthologs of Drosophila Class I genes) and gene expression changes after treatment of LCL cells with sulforaphane (SFN).

Article Snippet: Chemicals and materials D,L-sulforaphane (SFN) was purchased from MP Biomedicals LLC (Santa Ana, CA) or Calbiochem/Millipore (Billerica, MA) and ethyl 7-chloro-4-hydroxy-8-methylquinoline-3-carboxylate (AI-1) was purchased from Calbiochem/Millipore.

Techniques: Expressing

Journal: Free radical biology & medicine

Article Title: Beyond antioxidant genes in the ancient NRF2 regulatory network

doi: 10.1016/j.freeradbiomed.2015.06.044

Figure Lengend Snippet: (A) Human NRF2 ChIP-seq signal from LCL cells treated with DMSO or sulforaphane (SFN) as indicated. Select ancient NRF2 target genes with highly significant binding are represented (ChIP y-axis scale = 0-5000). (B) ChIP-seq signal as in (A) at select ancient NRF2 target genes with moderate binding (ChIP y-axis scale = 0-500). (C) Heatmap representing the response to sulforaphane (SFN), tert-butylhydroquinone (tBHQ), overexpression of NRF2, or overexpression of a dominant negative version of NRF2 (NRF2DN) for reporter constructs driven by the enhancer regions highlighted in panels (A) and (B). NQO1 is a positive control for human NRF2, but is not a conserved target because insects do not have an orthologous gene; the remaining nine are enhancers at deeply conserved NRF2 target genes.

Techniques: ChIP-sequencing, Binding Assay, Over Expression, Dominant Negative Mutation, Construct, Positive Control

(A) Structures of the ARE inducers used in panel B, shown as conjugates to Keap1 C151. The regioselectivity of the thiol addition at the endocyclic β-position of 15d-PGJ2 within the cyclopentenone ring has been established and is reviewed elsewhere [48]. Both Michael acceptor positions within CDDO-Im have been shown to contribute to ARE activation [61]. Only one conjugation is shown for simplicity, and the other Michael acceptor site is marked with an asterisk. (B) MDA-MB-231 cells were transfected with expression vectors for Nrf2, either wild-type Keap1 or Keap1 C151S, and a plasmid containing an ARE-dependent firefly luciferase reporter gene. A plasmid containing a constitutively expressed Renilla luciferase was included to normalize for plasmid levels. The transfected cells were exposed to either DMSO (vehicle), 5 μM sulforaphane, 50 nM CDDO-Im or 25 μM 15d-PGJ2 for 16 h prior to analysis of firefly and Renilla luciferase activities in cell lysates. The y-axis is the ratio of the firefly to Renilla values, and all data are normalized to wt Keap1 with DMSO.

Journal: The Biochemical journal

Article Title: Cul3-mediated Nrf2 ubiquitination and ARE activation are dependent on the partial molar volume at position 151 of Keap1

doi: 10.1042/BJ20090471

Figure Lengend Snippet: (A) Structures of the ARE inducers used in panel B, shown as conjugates to Keap1 C151. The regioselectivity of the thiol addition at the endocyclic β-position of 15d-PGJ2 within the cyclopentenone ring has been established and is reviewed elsewhere [48]. Both Michael acceptor positions within CDDO-Im have been shown to contribute to ARE activation [61]. Only one conjugation is shown for simplicity, and the other Michael acceptor site is marked with an asterisk. (B) MDA-MB-231 cells were transfected with expression vectors for Nrf2, either wild-type Keap1 or Keap1 C151S, and a plasmid containing an ARE-dependent firefly luciferase reporter gene. A plasmid containing a constitutively expressed Renilla luciferase was included to normalize for plasmid levels. The transfected cells were exposed to either DMSO (vehicle), 5 μM sulforaphane, 50 nM CDDO-Im or 25 μM 15d-PGJ2 for 16 h prior to analysis of firefly and Renilla luciferase activities in cell lysates. The y-axis is the ratio of the firefly to Renilla values, and all data are normalized to wt Keap1 with DMSO.

Article Snippet: Sulforaphane was purchased from MP Biomedicals, and the 1-[2-cyano-3-,12-dioxooleana-1,9(11)-dien-28-oyl]imidazole (CDDO-Im) was a generous gift from Michael Sporn (Dartmouth).

Techniques: Activation Assay, Conjugation Assay, Transfection, Expressing, Plasmid Preparation, Luciferase

(A) Structures of Cys, Ser and Trp, shown as a side chain at position C151. (B) Transient transfection reporter gene assays were conducted essentially as described for Figure 1. Cells were exposed to DMSO or sulforaphane for 16 h. The data for wt Keap1 and Keap1 C151S are the same as in Figure 1, shown here on a different scale, to compare with the ARE activity of the Keap1 C151W protein.

Journal: The Biochemical journal

Article Title: Cul3-mediated Nrf2 ubiquitination and ARE activation are dependent on the partial molar volume at position 151 of Keap1

doi: 10.1042/BJ20090471

Figure Lengend Snippet: (A) Structures of Cys, Ser and Trp, shown as a side chain at position C151. (B) Transient transfection reporter gene assays were conducted essentially as described for Figure 1. Cells were exposed to DMSO or sulforaphane for 16 h. The data for wt Keap1 and Keap1 C151S are the same as in Figure 1, shown here on a different scale, to compare with the ARE activity of the Keap1 C151W protein.

Article Snippet: Sulforaphane was purchased from MP Biomedicals, and the 1-[2-cyano-3-,12-dioxooleana-1,9(11)-dien-28-oyl]imidazole (CDDO-Im) was a generous gift from Michael Sporn (Dartmouth).

Techniques: Transfection, Activity Assay

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d l sulforaphane sfn (Valiant Co Ltd)

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