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Valiant Co Ltd lipoprotein cholesterol concentrate
Lipoprotein Cholesterol Concentrate, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 97/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0219147625/Lipoprotein-cholesterol+concentrate/bio_rxiv__2025__11__12__688119-224-26-29
Average 97 stars, based on 80 article reviews
lipoprotein cholesterol concentrate - by Bioz Stars, 2026-09
97/100 stars

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Article Title: Morphological and molecular characterisation of Orientia tsutsugamushi grown in tick cells
Article Snippet: The Ixodes scapularis embryo-derived cell line ISE6 ( ) was maintained at 32 °C in L-15B300 medium ( ) supplemented with 10% tryptose phosphate buffer (TPB), 10% fetal bovine serum (FBS; Thermo Scientific), 2 mM L-glutamine (Sigma-Aldrich), and 0.1% bovine lipoprotein concentrate (MP Biomedicals).

Article Title: Isolation, Molecular Characterization and In Vitro Propagation of an Anaplasma platys -Like Bacterium in Tick Cells
Article Snippet: The uninfected cells were grown in sealed T25 flasks at 28 °C in L-15B medium supplemented with 5% fetal bovine serum, 10% tryptose phosphate broth, 0.1% bovine lipoprotein concentrate (MP Biomedicals, Santa Ana, CA, USA), 2 mM L-glutamine, 100 IU/mL penicillin, and 100 μg/mL streptomycin.

Article Title: A 3D culture model facilitates mass production of in vitro Plasmodium falciparum haemolymph-like sporozoites.
Article Snippet: This C2 medium includes 54.5% v/v Schneider’s Drosophila Medium (21720024, ThermoFisher Scientific, US) supplemented with 10% v/v Insect Medium supplement (I7267, Sigma-Aldrich, MO), 0.1% v/v Trace Elements A (25-021-CI, Corning, US), 0.1% v/v Trace Elements C (25-023-CI), 0.1% v/v Trace Elements A (25-021-CI, Corning, US), 15% v/v FBS, 0.04% w/v NaHCO3 (BDH9280, VWR, US), 0.25% w/v trehalose (AC309870250 ThermoFisher Scientific, US), 1% v/v lipoprotein cholesterol concentrate (IC19147625, MP Biomedicals), 4% v/v human RBC lysate, 5 μg/mL silkworm haemolymph, 5 μM haemin chloride (3741, Sigma-Aldrich, MO), 0.022% v/v gentamicin (IB02030, VWR, US), and 1% v/v Penicillin/ Streptomycin solution (15140122, Gibco, US).

Article Title: A 3D culture model facilitates mass production of in vitro Plasmodium falciparum haemolymph-like sporozoites
Article Snippet: This C2 medium includes 54.5% v/v Schneider’s Drosophila Medium (21720024, ThermoFisher Scientific, US) supplemented with 10% v/v Insect Medium supplement (I7267, Sigma-Aldrich, MO), 0.1% v/v Trace Elements A (25-021-CI, Corning, US), 0.1% v/v Trace Elements C (25-023-CI), 0.1% v/v Trace Elements A (25-021-CI, Corning, US), 15% v/v FBS, 0.04% w/v NaHCO3 (BDH9280, VWR, US), 0.25% w/v trehalose (AC309870250 ThermoFisher Scientific, US), 1% v/v lipoprotein cholesterol concentrate (IC19147625, MP Biomedicals), 4% v/v human RBC lysate, 5 μg/mL silkworm haemolymph, 5 μM haemin chloride (3741, Sigma-Aldrich, MO), 0.022% v/v gentamicin (IB02030, VWR, US), and 1% v/v Penicillin/Streptomycin solution (15140122, Gibco, US).

Article Title:
Article Snippet: Leibovitz's L-15 Medium, powder Gibco 41300039 N/A L-aspartic acid Millipore-Sigma 11189 0.449 g/L L-glutamine Millipore-Sigma G8540 0.500 g/L L-proline Millipore-Sigma 81709 0.450 g/L L-glutamic acid Millipore-Sigma 49449 0.250 g/L alpha-ketoglutaric acid Millipore-Sigma K1128 0.449 g/L Sodium hydroxide Millipore-Sigma S8045 10 N D-glucose Millipore-Sigma G7021 18.018 g/L FBS (USDA approved; for tick media) Millipore-Sigma F0926-500ML 10% BactoTM Tryptose Phophate Broth BD 260300 10% Lipoprotein Concentrate MP Biomedicals 191476 0.1% Normal rabbit serum Pel-Freez #31126-5 6.0% Sodium bicarbonate Millipore-Sigma S6014 0.25% HEPES Millipore-Sigma H4034 25 mM CMRL1066 w/L-Glutamine (Powder) US Biological C5900 N/A Sodium citrate tribasic dihydrate Millipore-Sigma S4641 0.7 g/L Yeastolate BD 255772 2 g/L Neopeptone BD 211681 5 g/L N-Acetyl-α-D-glucosamine Millipore-Sigma 1079-25GM N/A Albumin, Bovine Fraction V MP Biomedicals 160069 N/A Rifampicin Millipore-Sigma 557303 50 ug/ml Phosphomycin Millipore-Sigma P5396 100 ug/ml Amphotericin B Gibco 15290-026 1:100 Sodium pyruvate Millipore-Sigma P5280 0.8 g/L Distilled water Gibco 15-230-147 N/A LB broth Millipore-Sigma L3022 N/A LB agar with 100 ug/ml ampicillin Quality Biological 50-751-7582 N/A Ampicillin Millipore-Sigma A0166 100 ug/ml Ammonium Chloride (15N, 99%) Cambridge Isotope NLM-467-10 0.6 g/L MOPS minimal media University of Maryland, Baltimore N/A N/A

Article Title: Tick Extracellular Vesicles Alter Epidermal Keratinocyte Function
Article Snippet: L15C300 medium was supplemented with 5% fetal bovine serum (Millipore-Sigma), 5% tryptose phosphate broth (BD Biosciences), 0.1% lipoprotein concentrate (MP Biomedicals), 0.25% sodium bicarbonate (Millipore-Sigma), and 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (Millipore-Sigma).

Article Title: Efficacy and immunological correlates of an mRNA-LNP vaccine for protection against an emerging rickettsial pathogen.
Article Snippet: Cell line ISE6 from embryos of the tick Ixodes scapularis [17] was maintained at 34°C in L15C300 medium supplemented with 5% each of FBS and Tryptose phosphate broth (DIFCO, Detroit, MI) and 0.2% bovine lipoprotein cholesterol concentrate (MP Biomedicals, Irvine, CA) [17].

Incubation:

Article Title: Adaptation of Anaplasma phagocytophilum to the tick vector is controlled by the transcriptional regulator Tr1
Article Snippet: .. I. scapularis (Say) embryonic tick cells (ISE6) were maintained in L15C-300 medium with 10% FBS (Sigma; F0926), 10% tryptone phosphate broth (TPB; BD; B260300) and 0.1% lipoprotein cholesterol concentrate (MP Biomedicals; 219147680) , in sealed tissue culture treated flasks, and incubated at 34°C . .. Infected ISE6 cell cultures were additionally supplemented with 0.25% NaHCO 3 and 25 mM HEPES buffer (Sigma), cultured in vented flasks, and kept at 34°C in a humidified chamber with 4% CO 2 .



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