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Valiant Co Ltd bleomycin sulfate

A single administration of <t>bleomycin</t> was used to induce pulmonary fibrosis in wildtype and fibulin 1c-deficient (fibulin-1 c−/−) mice that were sacrificed 28 days later. Control mice received PBS. (A) Representative images of lung tissue from both saline and bleomycin-exposed wildtype and fibulin-1 c−/− mice stained for OPG, scale bar = 300 µm. (B) Quantification of the average of positive OPG staining within the whole tissue (n= 7 or 8 mice). Each dot represents a mouse, and data are presented as the average of percentage of positive OPG-stained within the whole tissue. Groups were compared using a two-way ANOVA with Fisher Least Significant Difference post-hoc test and p<0.05 was considered significant. OPG: osteoprotegerin.

Bleomycin Sulfate, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0219030610/Bleomycin+sulfate/bio_rxiv__2024__12__05__626371-56-6-10
Average 96 stars, based on 14 article reviews

bleomycin sulfate - by Bioz Stars, 2026-09

96/100 stars

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1) Product Images from "Interaction of osteoprotegerin with fibulin-1 is essential for extracellular matrix deposition in fibrotic lung tissue"

Article Title: Interaction of osteoprotegerin with fibulin-1 is essential for extracellular matrix deposition in fibrotic lung tissue

Journal: bioRxiv

doi: 10.1101/2024.12.05.626371

A single administration of bleomycin was used to induce pulmonary fibrosis in wildtype and fibulin 1c-deficient (fibulin-1 c−/−) mice that were sacrificed 28 days later. Control mice received PBS. (A) Representative images of lung tissue from both saline and bleomycin-exposed wildtype and fibulin-1 c−/− mice stained for OPG, scale bar = 300 µm. (B) Quantification of the average of positive OPG staining within the whole tissue (n= 7 or 8 mice). Each dot represents a mouse, and data are presented as the average of percentage of positive OPG-stained within the whole tissue. Groups were compared using a two-way ANOVA with Fisher Least Significant Difference post-hoc test and p<0.05 was considered significant. OPG: osteoprotegerin.

Figure Legend Snippet: A single administration of bleomycin was used to induce pulmonary fibrosis in wildtype and fibulin 1c-deficient (fibulin-1 c−/−) mice that were sacrificed 28 days later. Control mice received PBS. (A) Representative images of lung tissue from both saline and bleomycin-exposed wildtype and fibulin-1 c−/− mice stained for OPG, scale bar = 300 µm. (B) Quantification of the average of positive OPG staining within the whole tissue (n= 7 or 8 mice). Each dot represents a mouse, and data are presented as the average of percentage of positive OPG-stained within the whole tissue. Groups were compared using a two-way ANOVA with Fisher Least Significant Difference post-hoc test and p<0.05 was considered significant. OPG: osteoprotegerin.

Techniques Used: Control, Saline, Staining

other:

Article Title: Ginkgo biloba Extract EGb761 Attenuates Bleomycin-Induced Experimental Pulmonary Fibrosis in Mice by Regulating the Balance of M1/M2 Macrophages and Nuclear Factor Kappa B (NF-κB)-Mediated Cellular Apoptosis
Article Snippet: The PF mouse model induced with BLM (MP Biomedicals, Shanghai, China) was performed as described before [33].

Control:

Article Title: HYDROGEL-BASED DELIVERY OF IL-10 IMPROVES TREATMENT OF BLEOMYCIN-INDUCED LUNG FIBROSIS IN MICE
Article Snippet: .. Two to four-month old wildtype C57Bl6J mice were treated with PBS control or bleomycin (MPBio, 0219030610) at a concentration of 1500–2000 international units/kg weight intratracheally; mice were anesthetized via ketamine/xylazine cocktail (K113–10ML, Sigma). ..

Concentration Assay:

Injection:

Article Title: Anti-Gal3 antibodies and uses thereof
Article Snippet: .. Briefly, C57Blck/6 male mice were injected intra-tracheally with 30 ug of Bleomycin Sulfate (MP biomedicals, cat #190306) reconstituted in 50 ul of PBS. .. Mice were treated on day 18, 20, 22, and 24 with mIgG2b isotype control, anti-GAL3 antibody (mIMT001) (10 mg/kg) by tail vein injection, or small molecule TD139 (Med Chem Express, cat #HY-19940) at 10 uM in 50 ul of Captisol (Med Chem Express, cat #HY-17031) injected intra-tracheally.

Article Title: Anti-GAL3 antibodies and uses thereof
Article Snippet: .. Briefly, C57Blck/6 male mice were injected intra-tracheally with 30 ug of Bleomycin Sulfate (MP biomedicals, cat #190306) reconstituted in 50 ul of PBS. .. Mice were treated on day 18, 20, 22, and 24 with mIgG2b isotype control, anti-GAL3 antibody (mIMT001) (10 mg/kg) by tail vein injection, or small molecule TD139 (Med Chem Express, cat #HY-19940) at 10 uM in 50 ul of Captisol (Med Chem Express, cat #HY-17031) injected intra-tracheally.

Article Title: Adiponectin inhibits the activation of lung fibroblasts and pulmonary fibrosis by regulating the nuclear factor kappa B (NF-κB) pathway
Article Snippet: .. BLM (1.5 U/kg; cat. No. 0219030610, MP Biomedicals, Santa Ana, CA, USA) was intraperitoneally injected into the mice [ ]. ..

Mouse Assay:

Image Search Results

A) ELISA quantification of IL-10 released from HH-10 formulation over 6 days (n=13). B)-C) Fluorescent microscopy images of lung tissue with fluorescently labeled HH reagent in both healthy (B) and bleomycin-challenged (C) lung tissue, displaying deposition locations of HH reagent via intranasal treatment. Images on right are high magnification of the squared regions.

Journal: Biomaterials

Article Title: HYDROGEL-BASED DELIVERY OF IL-10 IMPROVES TREATMENT OF BLEOMYCIN-INDUCED LUNG FIBROSIS IN MICE

doi: 10.1016/j.biomaterials.2019.02.017

Figure Lengend Snippet: A) ELISA quantification of IL-10 released from HH-10 formulation over 6 days (n=13). B)-C) Fluorescent microscopy images of lung tissue with fluorescently labeled HH reagent in both healthy (B) and bleomycin-challenged (C) lung tissue, displaying deposition locations of HH reagent via intranasal treatment. Images on right are high magnification of the squared regions.

Article Snippet: Two to four-month old wildtype C57Bl6J mice were treated with PBS control or bleomycin (MPBio, 0219030610) at a concentration of 1500–2000 international units/kg weight intratracheally; mice were anesthetized via ketamine/xylazine cocktail (K113–10ML, Sigma).

Techniques: Enzyme-linked Immunosorbent Assay, Microscopy, Labeling

A) Experimental design timeline of prevention cohort and analysis. B) Trichrome and α-SMA IHC stain of lung tissue sections by group of prevention cohorts, all parameters include bleomycin challenge. C) Sircol- assay quantifying collagen content of lung samples from healthy control and bleomycin challenged mice with preventative PBS, IL-10, HH, or HH-10. D) Ashcroft Score quantifying fibrosis of lung sections from healthy control and bleomycin challenged mice with preventative PBS, IL-10, HH, or HH-10. E) BAL cell counts from healthy control and bleomycin challenged mice with preventative PBS, IL-10, HH, or HH-10. For all graphs- ***p< .001 vs. PBS + BLM, **p< .01 vs. PBS + BLM, *p< .05 vs. PBS + BLM.

Journal: Biomaterials

Article Title: HYDROGEL-BASED DELIVERY OF IL-10 IMPROVES TREATMENT OF BLEOMYCIN-INDUCED LUNG FIBROSIS IN MICE

doi: 10.1016/j.biomaterials.2019.02.017

Figure Lengend Snippet: A) Experimental design timeline of prevention cohort and analysis. B) Trichrome and α-SMA IHC stain of lung tissue sections by group of prevention cohorts, all parameters include bleomycin challenge. C) Sircol- assay quantifying collagen content of lung samples from healthy control and bleomycin challenged mice with preventative PBS, IL-10, HH, or HH-10. D) Ashcroft Score quantifying fibrosis of lung sections from healthy control and bleomycin challenged mice with preventative PBS, IL-10, HH, or HH-10. E) BAL cell counts from healthy control and bleomycin challenged mice with preventative PBS, IL-10, HH, or HH-10. For all graphs- ***p< .001 vs. PBS + BLM, **p< .01 vs. PBS + BLM, *p< .05 vs. PBS + BLM.

Techniques: Staining

A) Experimental design timeline of treatment cohort and analysis. B) Trichrome IHC and α-SMA IHC stain of lung tissue sections of bleomycin-challenged treatment cohorts. C) Sircol- assay of lung samples from healthy control and bleomycin challenged mice with PBS, IL-10, HH, or HH-10 treatment. D) Ashcroft Score of lung sections from healthy control and bleomycin challenged mice with PBS, IL-10, HH, or HH-10 treatment. E) BAL cell counts from healthy control and bleomycin challenged mice with PBS, IL-10, HH, or HH-10 treatment. For all graphs- ***p< .001 vs. PBS + BLM, **p< .01 vs. PBS + BLM, *p< .05 vs. PBS + BLM.

Journal: Biomaterials

Article Title: HYDROGEL-BASED DELIVERY OF IL-10 IMPROVES TREATMENT OF BLEOMYCIN-INDUCED LUNG FIBROSIS IN MICE

doi: 10.1016/j.biomaterials.2019.02.017

Figure Lengend Snippet: A) Experimental design timeline of treatment cohort and analysis. B) Trichrome IHC and α-SMA IHC stain of lung tissue sections of bleomycin-challenged treatment cohorts. C) Sircol- assay of lung samples from healthy control and bleomycin challenged mice with PBS, IL-10, HH, or HH-10 treatment. D) Ashcroft Score of lung sections from healthy control and bleomycin challenged mice with PBS, IL-10, HH, or HH-10 treatment. E) BAL cell counts from healthy control and bleomycin challenged mice with PBS, IL-10, HH, or HH-10 treatment. For all graphs- ***p< .001 vs. PBS + BLM, **p< .01 vs. PBS + BLM, *p< .05 vs. PBS + BLM.

Techniques: Staining

A) IHC staining for phospho-Smad3 in bleomycin challenged mice treated in prevention or treatment regiments with PBS, IL-10, HH, or HH-10. B)-C) Quantification of pSmad3 positive cells from IHC images in bleomycin challenged mice treated in prevention or treatment regiments with PBS, IL-10, HH, or HH-10. For all graphs- ***p< .001 vs. PBS + BLM and **p< .01 vs. PBS + BLM.

Journal: Biomaterials

Article Title: HYDROGEL-BASED DELIVERY OF IL-10 IMPROVES TREATMENT OF BLEOMYCIN-INDUCED LUNG FIBROSIS IN MICE

doi: 10.1016/j.biomaterials.2019.02.017

Figure Lengend Snippet: A) IHC staining for phospho-Smad3 in bleomycin challenged mice treated in prevention or treatment regiments with PBS, IL-10, HH, or HH-10. B)-C) Quantification of pSmad3 positive cells from IHC images in bleomycin challenged mice treated in prevention or treatment regiments with PBS, IL-10, HH, or HH-10. For all graphs- ***p< .001 vs. PBS + BLM and **p< .01 vs. PBS + BLM.

Techniques: Immunohistochemistry

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bleomycin sulfate (Valiant Co Ltd)

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1) Product Images from "Interaction of osteoprotegerin with fibulin-1 is essential for extracellular matrix deposition in fibrotic lung tissue"

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