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Valiant Co Ltd calcein
Calcein, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0219016701/Calcein/pm41845010-313-0-1
Average 94 stars, based on 46 article reviews
calcein - by Bioz Stars, 2026-09
94/100 stars

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Related Articles

other:

Article Title: Cholesterol Attenuates the Pore-Forming Capacity of CARC-Containing Amphipathic Peptides.
Article Snippet: Calcein was from MP Biomedicals (Solon, OH, USA).

Article Title: Cholesterol Attenuates the Pore-Forming Capacity of CARC-Containing Amphipathic Peptides
Article Snippet: Calcein was from MP Biomedicals (Solon, OH, USA).

Construct:

Article Title: 3D Printing of a Vascularized Mini-Liver Based on the Size-Dependent Functional Enhancements of Cell Spheroids for Rescue of Liver Failure.
Article Snippet: Viability of 3D-Printed Endothelial Cells: To study the biocompatibility of the inks and 3D printing process, HUVECs were embedded in the external ink at a density of 1 × 106 cells mL−1 during 3D printing and cultured in a complete medium (DMEM/F12 1:1 basic medium (ThermoFisher, USA) supplemented with 10% fetal bovine serum (FBS, TBD, China) and 1× penicillin/streptomycin (Sigma-Aldrich, USA)) at 37 °C with 5% of CO2 supply. .. The 3D-printed constructs were incubated in a fresh basic medium containing 1 μm calcein-AM and 1 μm propidium iodide (PI, MP Biomedicals, France) at room temperature for 20 min on day 1. ..

Article Title: 3D Printing of a Vascularized Mini‐Liver Based on the Size‐Dependent Functional Enhancements of Cell Spheroids for Rescue of Liver Failure
Article Snippet: To study the biocompatibility of the inks and 3D printing process, HUVECs were embedded in the external ink at a density of 1 × 10 6 cells mL −1 during 3D printing and cultured in a complete medium (DMEM/F12 1:1 basic medium (ThermoFisher, USA) supplemented with 10% fetal bovine serum (FBS, TBD, China) and 1× penicillin/streptomycin (Sigma‐Aldrich, USA)) at 37 °C with 5% of CO 2 supply. .. The 3D‐printed constructs were incubated in a fresh basic medium containing 1 μ m calcein‐AM and 1 μ m propidium iodide (PI, MP Biomedicals, France) at room temperature for 20 min on day 1. ..

Incubation:

Article Title: 3D Printing of a Vascularized Mini-Liver Based on the Size-Dependent Functional Enhancements of Cell Spheroids for Rescue of Liver Failure.
Article Snippet: Viability of 3D-Printed Endothelial Cells: To study the biocompatibility of the inks and 3D printing process, HUVECs were embedded in the external ink at a density of 1 × 106 cells mL−1 during 3D printing and cultured in a complete medium (DMEM/F12 1:1 basic medium (ThermoFisher, USA) supplemented with 10% fetal bovine serum (FBS, TBD, China) and 1× penicillin/streptomycin (Sigma-Aldrich, USA)) at 37 °C with 5% of CO2 supply. .. The 3D-printed constructs were incubated in a fresh basic medium containing 1 μm calcein-AM and 1 μm propidium iodide (PI, MP Biomedicals, France) at room temperature for 20 min on day 1. ..

Article Title: Algorithmic construction of topologically complex biomineral lattices via cellular syncytia
Article Snippet: Post overnight incubation, samples were washed 4 times in PBST and mounted in Vectashield Antifade Mounting Medium (H-1000-10) before on glass slide/thin glass bottom dish (Pelco, Ted Pella Inc.) before performing confocal scans. .. For short time scale calcein labeling, live juveniles were incubated in 25 μg/ml concentration working solution of calcein (MP Biomedicals, 02190167-CF) in seawater. ..

Article Title: 3D Printing of a Vascularized Mini‐Liver Based on the Size‐Dependent Functional Enhancements of Cell Spheroids for Rescue of Liver Failure
Article Snippet: To study the biocompatibility of the inks and 3D printing process, HUVECs were embedded in the external ink at a density of 1 × 10 6 cells mL −1 during 3D printing and cultured in a complete medium (DMEM/F12 1:1 basic medium (ThermoFisher, USA) supplemented with 10% fetal bovine serum (FBS, TBD, China) and 1× penicillin/streptomycin (Sigma‐Aldrich, USA)) at 37 °C with 5% of CO 2 supply. .. The 3D‐printed constructs were incubated in a fresh basic medium containing 1 μ m calcein‐AM and 1 μ m propidium iodide (PI, MP Biomedicals, France) at room temperature for 20 min on day 1. ..

Labeling:

Article Title: Algorithmic construction of topologically complex biomineral lattices via cellular syncytia
Article Snippet: Post overnight incubation, samples were washed 4 times in PBST and mounted in Vectashield Antifade Mounting Medium (H-1000-10) before on glass slide/thin glass bottom dish (Pelco, Ted Pella Inc.) before performing confocal scans. .. For short time scale calcein labeling, live juveniles were incubated in 25 μg/ml concentration working solution of calcein (MP Biomedicals, 02190167-CF) in seawater. ..

Concentration Assay:

Article Title: Algorithmic construction of topologically complex biomineral lattices via cellular syncytia
Article Snippet: Post overnight incubation, samples were washed 4 times in PBST and mounted in Vectashield Antifade Mounting Medium (H-1000-10) before on glass slide/thin glass bottom dish (Pelco, Ted Pella Inc.) before performing confocal scans. .. For short time scale calcein labeling, live juveniles were incubated in 25 μg/ml concentration working solution of calcein (MP Biomedicals, 02190167-CF) in seawater. ..

Article Title: Ocean acidification disrupts the biomineralization process in the oyster Crassostrea virginica via intracellular calcium signaling dysregulation.
Article Snippet: .. Calcein (MP Biomedicals), a calcium-dependent fluorescent signal (UV channel, Exc: 408 nm/Em: 450–490 nm), was utilized for CaCO3 staining of the calcified shell (final concentration 10 mM in 0.01% DMSO). .. Calcofluor White Stain (Sigma-Aldrich), a chitin-staining fluorescent signal (FITC channel, Exc: 488 nm/Em: 520-560 nm), was used to visualize the shell organic matrix (final concentration 0.02 mM in 0.01% DMSO).

Staining:

Article Title: Ocean acidification disrupts the biomineralization process in the oyster Crassostrea virginica via intracellular calcium signaling dysregulation.
Article Snippet: .. Calcein (MP Biomedicals), a calcium-dependent fluorescent signal (UV channel, Exc: 408 nm/Em: 450–490 nm), was utilized for CaCO3 staining of the calcified shell (final concentration 10 mM in 0.01% DMSO). .. Calcofluor White Stain (Sigma-Aldrich), a chitin-staining fluorescent signal (FITC channel, Exc: 488 nm/Em: 520-560 nm), was used to visualize the shell organic matrix (final concentration 0.02 mM in 0.01% DMSO).



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