arthrobacter luteus yeast lytic enzyme (Valiant Co Ltd)

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Valiant Co Ltd arthrobacter luteus yeast lytic enzyme
Arthrobacter Luteus Yeast Lytic Enzyme, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0219012301/Yeast+lytic+enzyme/10__1074_slash_jbc__m704707200-62-15-21
Average 96 stars, based on 32 article reviews

arthrobacter luteus yeast lytic enzyme - by Bioz Stars, 2026-09

96/100 stars

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Immunofluorescence:

Article Title: Direct recruitment of Mis18 to interphase spindle pole bodies promotes CENP-A chromatin assembly.
Article Snippet: .. Immunofluorescence Cells were fixed in 3.7% formaldehyde for 15 min at RT, then washed 2x in PEM (100 mM PIPES, pH 7.0, 1 mM EDTA, 1 mMMgCl2), 1x in PEMS (PEM + 1.2 M sorbitol), and stored in PEM + 0.1% sodium azide at 4 C. Fixed cells were treated with 1 mg/mL zymolyase T100 (MPBiomedicals cat #08320932) at 37 C for 90min, washed in PEMS, then permeabilized in PEMS + 1%Triton-X100 for 1min. ..

Recombinant:

Article Title: An Essential and Cell-Cycle-Dependent ORC Dimerization Cycle Regulates Eukaryotic Chromosomal DNA Replication.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Orc3 (Mouse) Gift from Prof. Bruce Stillman (CSH) N/A Anti-Orc6 (Mouse) Gift from Prof. Bruce Stillman (CSH) N/A Anti-Mcm2 (Mouse) Gift from Prof. Bruce Stillman (CSH) N/A Anti-Cdc6 (Goat) Santa Cruz Biotechnology, Inc. Cat#sc-6317; RRID:AB_638343 Anti-HA (Mouse) Roche Diagnostics Cat#11666606001: RRID:AB_514506 Anti-Myc (Mouse) Roche Diagnostics Cat#11667203001; RRID:AB_390911 Anti-FLAG M2 (Mouse) Sigma-Aldrich Cat# F3165; RRID:AB_259529 Anti-Histone H3 antibody (Rabbit) Abcam Cat#ab1791; RRID:AB_302613 Anti-beta Actin antibody - Loading Control Abcam Cat#ab8226; RRID:AB_306371 Donkey anti-Goat IgG (H+L) Secondary Antibody, HRP ThermoFisher Scientific, Inc. Cat#A15999; RRID:AB_2763439 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, HRP ThermoFisher Scientific, Inc. Cat#A16110; RRID:AB_2534782 Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP ThermoFisher Scientific, Inc. Cat#31430; RRID:AB_228307 Peroxidase IgG Fraction Monoclonal Mouse AntiRabbit IgG, light chain specific Jackson ImmunoResearch Laboratories, Inc. Cat#211-032-171; RRID:AB_2339149 Peroxidase AffiniPure Goat Anti-Mouse IgG, light chain specific Jackson ImmunoResearch Laboratories, Inc. Cat#115-035-174; RRID:AB_2338512 Bacterial and Virus Strains Subcloning Efficiency DH5a Competent Cells ThermoFisher Scientific, Inc. Cat# 18265017 Chemicals, Peptides, and Recombinant Proteins Hydroxyurea Sigma-Aldrich Cat# H8627 Nocodozole Sigma-Aldrich Cat# M1404-50MG Alpha-Factor Sangon Biotech (Shanghai) Co., Ltd. .. Custom Synthesis SuperSignal West Pico PLUS ThermoFisher Scientific, Inc. Cat# 34580 Rec-Protein G - Sepharose 4B Conjugate Invitrogen Cat# 101243 Formaldehyde solution Sigma-Aldrich Cat# 252549-2.5L Glycine Ultrapure MB Grade Affymetrix, Inc. Cat# 16407 5 KG BioRad - Protein Assay Dye Reagent Concentrate Bio-Rad Laboratories, Inc. Cat# 500-0006 DNase I recombinant, RNase-free Roche Diagnostics Cat# 04716728001 Yeast Lytic Enzyme MP Biomedicals Cat# 02153526-CF Alkaline Phosphatase New England BioLabs, Inc. Cat# M0290 Beta-galactosidase Roche Diagnostics Cat# 10105031001 Experimental Models: Organisms/Strains Saccharomyces cerevisiae. .. NCBI NCBI:txid4896 W303-1A: MATalpha ade2-1 ura3-1 his3-11 trp1-1 leu2-3 leu2-112 can1-100 ATCC ATCC: 208353 See Table S1 for full strain list.

Article Title: Design, construction, and functional characterization of a tRNA neochromosome in yeast.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Escherichia coli DH5a Invitrogen Cat# 18258012 Escherichia coli Top10 Invitrogen Cat# C4040-10 Chemicals, peptides, and recombinant proteins 1 kb Plus DNA Ladder New England Biolabs (NEB) Cat# N3200L 5-Fluoroorotic acid (5-FOA) Formedium Cat# 5FOA10 Agencourt AMPure XP Beckman Coulter Cat# A63881/A63882 Biotin-14-dCTP Fisher Scientific Cat# 10022582 Certified Megabase Agarose Bio-Rad Cat# 1613108 cOmplete Protease inhibitor-cocktail Roche Cat# 4693116001 Covaris microTUBE Snap-Cap AFA Fiber Covaris Cat# 520045 DMSO Sigma Aldrich Cat# D8418-100ML Deoxynucleotide (dNTP) Solution Set NEB Cat# N0446S DreamTaq Green PCR Master Mix (2X) Thermo Fisher Cat# K1082 Dynabeads Streptavidin C1 Fisher Scientific Cat# 10202333 Formaldehyde Sigma Aldrich Cat# F8775 Glycine Sigma Cat# G7126 GoTaq G2 Green Master Mix Promega UK Limited Cat# M7823 Herring sperm DNA Promega UK Limited Cat# D1815 Lambda PFG Ladder NEB Cat# N0341S Lithium acetate dihydrate Sigma Aldrich Cat# L4158-250G Lyticase Sigma Aldrich Cat# L2524-50KU N-Lauroylsarcosine sodium salt Sigma Aldrich Cat# L9150-100G PEG 3350 Sigma Aldrich Cat# P4338-1KG PEG 8000 Sigma Aldrich Cat# P2139-500G PhosSTOP Roche Cat# 4906845001 Phusion polymerase NEB Cat# M0530L Plasmid-Safe ATP-Dependent DNase Epicentre Cat# E3101K Primary antibody: a-Tubulin Cell Signaling Technology Cat# 2144; RRID:AB_2210548 Primary antibody: Anti-HA Sigma Aldrich Cat# H6908; RRID:AB_260070 Primary antibody: Phospho-eIF2a (Ser51) Cell Signaling Technology Cat# 3398; RRID:AB_2096481 Proteinase K from Tritirachium album Sigma Aldrich Cat# P4850-1ML Q5 polymerase NEB Cat# M0491L Restriction enzymes NEB N/A RNase A Qiagen Cat# 19101 RNase cocktail Invitrogen Cat# AM2286 Rubidium chloride Sigma Aldrich Cat# R2252-100G SeaKem LE agarose Lonza Cat# 50001 Secondary antibody: Anti-rabbit IgG, HRP-linked Cell Signaling Technology Cat# 7074; RRID:AB_2099233 Sodium deoxycholate Sigma Aldrich Cat# D6750-100G Sytox Green Invitrogen Cat# S7020 T4 DNA Ligase New England Biolabs Cat# M0202L T4 DNA Polymerase New England Biolabs Cat# M0203L T5 exonuclease NEB Cat# M0663S (Continued on next page) e1 Cell 186, 5237–5253.e1–e10, November 22, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Taq ligase NEB Cat# M0208L Zymolyase 20T from Arthrobacter luteus MP Bio Cat# MP08320921 Experimental models: organisms/strains See Methods S1 for Saccharomyces cerevisiae strains used in this study This study N/A Oligonucleotides ‘PCR Tags’ are listed in Methods S1 IDT N/A Recombinant DNA Plasmids are listed in Methods S1 and sequences are provided as Genbank files in Data S4. .. N/A N/A Software and algorithms Bowtie2 Langmead and Salzberg69 and Langmead et al.70 http://bowtie-bio.sourceforge.net/ bowtie2/index.shtml cutadapt v1.15 and v4.4 Kechin et al.71,72 https://cutadapt.readthedocs.io/ en/stable/ DEseq2 v1.30.1 Anders and Huber73 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html FastQC v0.10.1 and v0.11.9 de Sena Brandine and Smith74 https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ featureCounts v2.0.1 Liao et al.75 N/A Hisat2 v2.1.0 Kim et al.76 http://daehwankimlab.github.io/hisat2/ GATK v2.7 McKenna et al.77 https://gatk.broadinstitute.org/hc/en-us Growthcurver Sprouffske and Wagner78 https://github.com/cran/growthcurver Guppy Oxford Nanopore Technologies https://nanoporetech.com/ IGV Viewer and IGV 2.5.2 Robinson et al.79 https://software.broadinstitute.org/ software/igv/ IQuant Wen et al.80 N/A Je Girardot et al.81 https://gbcs.embl.de/portal/ tiki-index.php?page=Je Mascot Matrix Science Inc https://www.matrixscience.com/ Minimap2 Li82 https://github.com/lh3/minimap2 NUCwave Quintales et al.83 http://nucleosome.usal.es/nucwave/ PySam Li et al.84 https://github.com/pysam-developers/pysam R / RStudio R studio https://www.r-project.org/ https://www.rstudio.com/ Salmon v1.2.1 Patro et al.85 N/A Samtools Li et al.84 and Danecek et al.86 http://www.htslib.org/ Sleuth v0.30.0 Pimentel et al.87 https://pachterlab.github.io/sleuth/ Snapgene GSL Biotech LLC http://www.snapgene.com/ SOAPnuke Chen et al.88 N/A Tablet v1.21.02 Milne et al.89 N/A tRNAscan-SE v.2.0 Lowe and Chan90 http://lowelab.ucsc.edu/tRNAscan-SE/ Deposited data Code for tRNA gene assignment to flanking sequences This study https://github.com/the-cai-lab/tRNA-neochr Raw sequencing reads This study NCBI BioProject: PRJNA884518 Proteomics raw data This study iProX: PXD037209 MSR-seq raw data This study NCBI GEO: GSE243449 ll OPEN ACCESS Cell 186, 5237–5253.e1–e10, November 22, 2023 e2 Article ll OPEN ACCESS Article

Centrifugation:

Article Title: Diverse DNA Sequence Motifs Activate Meiotic Recombination Hotspots Through a Common Chromatin Remodeling Pathway
Article Snippet: .. Cells were collected by centrifugation at 4°, then resuspended in 3.3 ml of spheroplast buffer (1 M Sorbitol, 50 mM Tris-HCl, pH 7.4) supplemented with 2.3 μl of BME and 50 μl of yeast lytic enzyme (100 mg/ml; MP Biomedicals, Santa Anna, CA). ..

Article Title: Diverse DNA Sequence Motifs Activate Meiotic Recombination Hotspots Through a Common Chromatin Remodeling Pathway.
Article Snippet: .. Cells were collected by centrifugation at 4 , then resuspended in 3.3 ml of spheroplast buffer (1 M Sorbitol, 50 mM Tris-HCl, pH 7.4) supplemented with 2.3 ml of BME and 50 ml of yeast lytic enzyme (100 mg/ml; MP Biomedicals, Santa Anna, CA). ..

Polymerase Chain Reaction:

other:

Article Title: Cell nuclei extraction technology
Article Snippet: Afterwards, take 3 ml out of this solution and mixed with 30 mg ImmunO Yeast Lytic Enzyme (MP Biomedicals, lnc),then placed at 30℃ incubator shaking 1 hour 30 minutes.

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