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nuclease p1  (Valiant Co Ltd)

 
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    Structured Review

    Valiant Co Ltd nuclease p1
    Nuclease P1, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/0218055501/Nuclease+P1/10__1128_slash_jvi__79__12__7327___7337__2005-106-16-40
    Average 96 stars, based on 54 article reviews
    nuclease p1 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Structural and Mechanistic Insights into the Transport of Aristolochic Acids and their Active Metabolites by Human Serum Albumin.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Structural and mechanistic insights into the transport of aristolochic acids and their active metabolites by human serum albumin
    Article Snippet: .. Enzymes were obtained from the following sources: Micrococcal nuclease ( Staphylococcus aureus ) and potato apyrase were purchased from Sigma Aldrich; Bovine spleen phosphodiesterase II and nuclease P1 ( Penicillium citrinum ) were purchased from MP Biomedicals; and polynucleotide kinase lacking 3′-phosphatase activity was purchased from New England Biolabs. .. Gamma- 32 P-ATP (6000 Ci/mmol; 10 μCi/μl) was obtained from PerkinElmer.

    Centrifugation:

    Article Title: Characterization of ADAT2/3 molecules in Trypanosoma cruzi and regulation of mucin gene expression by tRNA editing
    Article Snippet: .. After centrifugation, the pellet was dissolved in 30 mM ammonium acetate, 10 mM Zn++ acetate containing 0.4 units of nuclease P1 (MPBiomedicals) in a 20 ml reaction and incubated at 37°C for at least 12 h. The reaction was dried down in a SpeedVacDNA110 concentrator system (Savant) for 10 min under high heat and resuspended in 3 ml of double-distilled H2O, where 1 ml (13.33 pmol) was spotted and dried individually onto a cellulose thin-layer chromatography (TLC) sheet (EMD Chemicals). ..

    Incubation:

    Article Title: Characterization of ADAT2/3 molecules in Trypanosoma cruzi and regulation of mucin gene expression by tRNA editing
    Article Snippet: .. After centrifugation, the pellet was dissolved in 30 mM ammonium acetate, 10 mM Zn++ acetate containing 0.4 units of nuclease P1 (MPBiomedicals) in a 20 ml reaction and incubated at 37°C for at least 12 h. The reaction was dried down in a SpeedVacDNA110 concentrator system (Savant) for 10 min under high heat and resuspended in 3 ml of double-distilled H2O, where 1 ml (13.33 pmol) was spotted and dried individually onto a cellulose thin-layer chromatography (TLC) sheet (EMD Chemicals). ..

    Thin Layer Chromatography:

    Article Title: Characterization of ADAT2/3 molecules in Trypanosoma cruzi and regulation of mucin gene expression by tRNA editing
    Article Snippet: .. After centrifugation, the pellet was dissolved in 30 mM ammonium acetate, 10 mM Zn++ acetate containing 0.4 units of nuclease P1 (MPBiomedicals) in a 20 ml reaction and incubated at 37°C for at least 12 h. The reaction was dried down in a SpeedVacDNA110 concentrator system (Savant) for 10 min under high heat and resuspended in 3 ml of double-distilled H2O, where 1 ml (13.33 pmol) was spotted and dried individually onto a cellulose thin-layer chromatography (TLC) sheet (EMD Chemicals). ..



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    Representative autoradiographic profiles of AA–DNA adducts detected by <t>the</t> <t>nuclease</t> <t>P1</t> enrichment version of the 32P-postlabeling assay. Electronic autoradiography was performed for 5–10 min. Ori-gins in the bottom left corners were cut off before exposure. (A) Liver control; (B) AA-treated liver; (C) kidney control; (D) AA-treated kid-ney; spot (1) dA–AAI; spot (2) dG–AAI; spot (3) dA–AAII.
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    Image Search Results


    Representative autoradiographic profiles of AA–DNA adducts detected by the nuclease P1 enrichment version of the 32P-postlabeling assay. Electronic autoradiography was performed for 5–10 min. Ori-gins in the bottom left corners were cut off before exposure. (A) Liver control; (B) AA-treated liver; (C) kidney control; (D) AA-treated kid-ney; spot (1) dA–AAI; spot (2) dG–AAI; spot (3) dA–AAII.

    Journal: Mutation research

    Article Title: DNA adduct formation and mutation induction by aristolochic acid in rat kidney and liver

    doi: 10.1016/j.mrfmmm.2006.08.004

    Figure Lengend Snippet: Representative autoradiographic profiles of AA–DNA adducts detected by the nuclease P1 enrichment version of the 32P-postlabeling assay. Electronic autoradiography was performed for 5–10 min. Ori-gins in the bottom left corners were cut off before exposure. (A) Liver control; (B) AA-treated liver; (C) kidney control; (D) AA-treated kid-ney; spot (1) dA–AAI; spot (2) dG–AAI; spot (3) dA–AAII.

    Article Snippet: For nuclease P1 enrichment, the digests were incubated with 4 μg nuclease P1 (MP Biomedicals, London, UK) in 3 μl of a buffer containing 0.8 M sodium acetate (pH 5.0) and 2 mM zinc chloride for 30 min at 37 °C.

    Techniques: Postlabeling Assay, Autoradiography