Migration:Article Title: Upregulation of the lncRNA MEG3 in Metastatic Hepatoblastoma.
Article Snippet: .. For migration assays, the bottom of Transwell inserts (8 μm pores, Corning Life Sciences, Corning, NY, USA) was coated with Type I collagen (10 μg/mL, MP Biomedicals, Santa Ana, CA, USA) for HLM_2 and HuH6 cells or fibronectin (10 μg/mL, Qiagen, Germantown, MD, USA) for COA67 cells for 4 h at 37 ◦C. .. Additionally, for invasion assays, the tops of the inserts were coated with 80 μL of Matrigel (1 mg/mL, BD Biosciences, San Jose, CA, USA) for 4 h at 37 ◦C.
Article Title: Upregulation of the lncRNA MEG3 in Metastatic Hepatoblastoma
Article Snippet: .. For migration assays, the bottom of Transwell inserts (8 μm pores, Corning Life Sciences, Corning, NY, USA) was coated with Type I collagen (10 μg/mL, MP Biomedicals, Santa Ana, CA, USA) for HLM_2 and HuH6 cells or fibronectin (10 μg/mL, Qiagen, Germantown, MD, USA) for COA67 cells for 4 h at 37 °C. .. Additionally, for invasion assays, the tops of the inserts were coated with 80 μL of Matrigel (1 mg/mL, BD Biosciences, San Jose, CA, USA) for 4 h at 37 °C.
Membrane:Article Title: Dichotomous SMAD2/3 regulation and selective antihypertrophic activity of heparin during in vitro chondrogenesis of mesenchymal stromal cells
Article Snippet: .. For collagen detection, the lower part of the membrane was incubated with mouse anti-human type X collagen antibody (1:500, clone X53, no. 41-9771-82, Invitrogen) and the upper part with mouse anti-human type II collagen antibody (1:1000, clone II-4C11, no. 63171, MP Biomedicals, Eschwege, Germany). .. For the detection of SMAD, AKT, or β-catenin, the upper membrane sections were treated with rat monoclonal anti-pSMAD1/9 (1:1000, pS463/pS465, pS465/pS467, clone N6-1233 (RUO), no. 562509, BD Biosciences, East Rutherford, NJ, USA), rabbit monoclonal anti-pSMAD2 (1:500, pS465/pS467, clone 138D4, no. 3108), rabbit monoclonal anti-pSMAD3 (1:500, pS423/pS425, clone C25A9, no. 9520), rabbit polyclonal anti-pAKT (1:500, pS473, no. 9721, all Cell Signaling Technology, Danvers, MA, USA), or mouse monoclonal anti-β-catenin antibodies (1:10,000, clone 14/Beta-Catenin (RUO), no. 610154, BD Biosciences).
Article Title: Dichotomous SMAD2/3 regulation and selective antihypertrophic activity of heparin during in vitro chondrogenesis of mesenchymal stromal cells.
Article Snippet: .. For collagen detection, the lower part of the membrane was incubated with mouse antihuman type X collagen antibody (1:500, clone X53, no. 41-9771-82, Invitrogen) and the upper part with mouse anti-human type II collagen antibody (1:1000, clone II-4C11, no. 63171, MP Biomedicals, Eschwege, Germany). .. For the detection of SMAD, AKT, or β-catenin, the upper membrane sections were treated with rat monoclonal antipSMAD1/9 (1:1000, pS463/pS465, pS465/pS467, clone N6-1233 (RUO), no. 562509, BD Biosciences, East Rutherford, NJ, USA), rabbit monoclonal anti-pSMAD2 (1:500, pS465/ pS467, clone 138D4, no. 3108), rabbit monoclonal anti-pSMAD3 (1:500, pS423/pS425, clone C25A9, no. 9520), rabbit polyclonal anti-pAKT (1:500, pS473, no. 9721, all Cell Signaling Technology, Danvers, MA, USA), or mouse monoclonal anti-β-catenin antibodies (1:10,000, clone 14/Beta-Catenin (RUO), no. 610154, BD Biosciences).
Incubation:Article Title: Dichotomous SMAD2/3 regulation and selective antihypertrophic activity of heparin during in vitro chondrogenesis of mesenchymal stromal cells
Article Snippet: .. For collagen detection, the lower part of the membrane was incubated with mouse anti-human type X collagen antibody (1:500, clone X53, no. 41-9771-82, Invitrogen) and the upper part with mouse anti-human type II collagen antibody (1:1000, clone II-4C11, no. 63171, MP Biomedicals, Eschwege, Germany). .. For the detection of SMAD, AKT, or β-catenin, the upper membrane sections were treated with rat monoclonal anti-pSMAD1/9 (1:1000, pS463/pS465, pS465/pS467, clone N6-1233 (RUO), no. 562509, BD Biosciences, East Rutherford, NJ, USA), rabbit monoclonal anti-pSMAD2 (1:500, pS465/pS467, clone 138D4, no. 3108), rabbit monoclonal anti-pSMAD3 (1:500, pS423/pS425, clone C25A9, no. 9520), rabbit polyclonal anti-pAKT (1:500, pS473, no. 9721, all Cell Signaling Technology, Danvers, MA, USA), or mouse monoclonal anti-β-catenin antibodies (1:10,000, clone 14/Beta-Catenin (RUO), no. 610154, BD Biosciences).
Article Title: Dichotomous SMAD2/3 regulation and selective antihypertrophic activity of heparin during in vitro chondrogenesis of mesenchymal stromal cells.
Article Snippet: .. For collagen detection, the lower part of the membrane was incubated with mouse antihuman type X collagen antibody (1:500, clone X53, no. 41-9771-82, Invitrogen) and the upper part with mouse anti-human type II collagen antibody (1:1000, clone II-4C11, no. 63171, MP Biomedicals, Eschwege, Germany). .. For the detection of SMAD, AKT, or β-catenin, the upper membrane sections were treated with rat monoclonal antipSMAD1/9 (1:1000, pS463/pS465, pS465/pS467, clone N6-1233 (RUO), no. 562509, BD Biosciences, East Rutherford, NJ, USA), rabbit monoclonal anti-pSMAD2 (1:500, pS465/ pS467, clone 138D4, no. 3108), rabbit monoclonal anti-pSMAD3 (1:500, pS423/pS425, clone C25A9, no. 9520), rabbit polyclonal anti-pAKT (1:500, pS473, no. 9721, all Cell Signaling Technology, Danvers, MA, USA), or mouse monoclonal anti-β-catenin antibodies (1:10,000, clone 14/Beta-Catenin (RUO), no. 610154, BD Biosciences).
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