Affinity Chromatography:Article Title: Glomerular basement membrane structural integrity dictates trans-tissue deposition of laminin in the kidney.
Article Snippet: Compound Sakura Finetek 4583 4× Laemmli sample buffer Bio-Rad 1610747 2× Laemmli sample buffer Bio-Rad 1610737 CanGet Signal solution 1 TOYOBO NKB-201 CanGet Signal solution 2 TOYOBO NKB-301 ECL Select Western Blotting Detection Reagent Cytiva RPN2235 RIPA buffer containing protease/ phosphatase inhibitor Cell Signaling 5872S Recombinant human laminin-211 protein Peter D. Yurchenco Lab NA Furin inhibitor 1 Calbiochem 344930 (Continued on next page) 16 Cell Reports 45, 117006, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Heparin affinity chromatography (heparin agarose) Sigma H6508 Adriamycin (Doxorubicin hydrochloride) MP Biomedicals 159101 Critical commercial assays BCA protein assay kit Pierce 23225 QIAquick PCR Purification Kit QIAGEN 28106 PureLinkTM HiPure Plasmid Midiprep Kit Invitrogen K210005 Wizard SV Gel and PCR Clean-Up System Promega A9281 the Quick-DNA Microprep Kit Zymo Research D3020 Hoechst 33258 Invitrogen H3569 Deposited data Human Plasma PeptideAtlas Institute for Systems Biology Human Plasma 2025-08 Experimental models: Cell lines .. Neuro-2a ATCC CCL-131; RRID:CVCL_0470 C2C12 Clay F. Semenkovich Lab NA Experimental models: Organisms/strains Rosa26-Cas9 knockin Jackson Laboratory 026179 Rosa26-LSL-Cas9 knockin Jackson Laboratory 026175 Igs2-dCas9-KRAB knockin Jackson Laboratory 030000 Hopx-CreERT2 knockin Jackson Laboratory 017606 Ai14 knockin Jackson Laboratory 007914 Col4a5-G5X mutant Yoav Segal, University of Minnesota NA Pax3-Cre Transgenic Jonathan Epstein, University of Pennsylvania NA Hopx-Cre knockin Jonathan Epstein, University of Pennsylvania NA Nphs2-Cre Transgenic Larry Holzman, University of Pennsylvania NA Lamb2 knockout Jeffrey H. Miner Lab NA Cd2ap knockout Andrey Shaw, Genentech NA Tg: Lama2-gRNA Transgenic This study NA Oligonucleotides Lama2 KO sgRNA #1; ACCCGAGATGTACTGCAAGT This study; IDT NA Lama2 KO sgRNA #2; TACAATATCTATCCCCGCAC This study; IDT NA Lama2 KO sgRNA #3; GGCTGCCTTCACAATTACGT This study; IDT NA Lama2 KO sgRNA #4; GGAACGTTCCCTGGATGACG This study; IDT NA Lama2 KO sgRNA #5; GTGCATTCGAAGCAAGATTC This study; IDT NA Lama2 KO sgRNA #6; AGTTGGTGGAACATGTCCCC This study; IDT NA Lama2 KO sgRNA #7; ATGACGTGGAGTACAAACCC This study; IDT NA Lama2 KO sgRNA #8; CGAGATGTACTGCAAGTTGG This study; IDT NA Lama2 KO sgRNA #9; CTGCTCTTGGGGACGCTCGA This study; IDT NA (Continued on next page) Cell Reports 45, 117006, March 24, 2026 17
Bicinchoninic Acid Protein Assay:Article Title: Glomerular basement membrane structural integrity dictates trans-tissue deposition of laminin in the kidney.
Article Snippet: Compound Sakura Finetek 4583 4× Laemmli sample buffer Bio-Rad 1610747 2× Laemmli sample buffer Bio-Rad 1610737 CanGet Signal solution 1 TOYOBO NKB-201 CanGet Signal solution 2 TOYOBO NKB-301 ECL Select Western Blotting Detection Reagent Cytiva RPN2235 RIPA buffer containing protease/ phosphatase inhibitor Cell Signaling 5872S Recombinant human laminin-211 protein Peter D. Yurchenco Lab NA Furin inhibitor 1 Calbiochem 344930 (Continued on next page) 16 Cell Reports 45, 117006, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Heparin affinity chromatography (heparin agarose) Sigma H6508 Adriamycin (Doxorubicin hydrochloride) MP Biomedicals 159101 Critical commercial assays BCA protein assay kit Pierce 23225 QIAquick PCR Purification Kit QIAGEN 28106 PureLinkTM HiPure Plasmid Midiprep Kit Invitrogen K210005 Wizard SV Gel and PCR Clean-Up System Promega A9281 the Quick-DNA Microprep Kit Zymo Research D3020 Hoechst 33258 Invitrogen H3569 Deposited data Human Plasma PeptideAtlas Institute for Systems Biology Human Plasma 2025-08 Experimental models: Cell lines .. Neuro-2a ATCC CCL-131; RRID:CVCL_0470 C2C12 Clay F. Semenkovich Lab NA Experimental models: Organisms/strains Rosa26-Cas9 knockin Jackson Laboratory 026179 Rosa26-LSL-Cas9 knockin Jackson Laboratory 026175 Igs2-dCas9-KRAB knockin Jackson Laboratory 030000 Hopx-CreERT2 knockin Jackson Laboratory 017606 Ai14 knockin Jackson Laboratory 007914 Col4a5-G5X mutant Yoav Segal, University of Minnesota NA Pax3-Cre Transgenic Jonathan Epstein, University of Pennsylvania NA Hopx-Cre knockin Jonathan Epstein, University of Pennsylvania NA Nphs2-Cre Transgenic Larry Holzman, University of Pennsylvania NA Lamb2 knockout Jeffrey H. Miner Lab NA Cd2ap knockout Andrey Shaw, Genentech NA Tg: Lama2-gRNA Transgenic This study NA Oligonucleotides Lama2 KO sgRNA #1; ACCCGAGATGTACTGCAAGT This study; IDT NA Lama2 KO sgRNA #2; TACAATATCTATCCCCGCAC This study; IDT NA Lama2 KO sgRNA #3; GGCTGCCTTCACAATTACGT This study; IDT NA Lama2 KO sgRNA #4; GGAACGTTCCCTGGATGACG This study; IDT NA Lama2 KO sgRNA #5; GTGCATTCGAAGCAAGATTC This study; IDT NA Lama2 KO sgRNA #6; AGTTGGTGGAACATGTCCCC This study; IDT NA Lama2 KO sgRNA #7; ATGACGTGGAGTACAAACCC This study; IDT NA Lama2 KO sgRNA #8; CGAGATGTACTGCAAGTTGG This study; IDT NA Lama2 KO sgRNA #9; CTGCTCTTGGGGACGCTCGA This study; IDT NA (Continued on next page) Cell Reports 45, 117006, March 24, 2026 17
Polymerase Chain Reaction:Article Title: Glomerular basement membrane structural integrity dictates trans-tissue deposition of laminin in the kidney.
Article Snippet: Compound Sakura Finetek 4583 4× Laemmli sample buffer Bio-Rad 1610747 2× Laemmli sample buffer Bio-Rad 1610737 CanGet Signal solution 1 TOYOBO NKB-201 CanGet Signal solution 2 TOYOBO NKB-301 ECL Select Western Blotting Detection Reagent Cytiva RPN2235 RIPA buffer containing protease/ phosphatase inhibitor Cell Signaling 5872S Recombinant human laminin-211 protein Peter D. Yurchenco Lab NA Furin inhibitor 1 Calbiochem 344930 (Continued on next page) 16 Cell Reports 45, 117006, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Heparin affinity chromatography (heparin agarose) Sigma H6508 Adriamycin (Doxorubicin hydrochloride) MP Biomedicals 159101 Critical commercial assays BCA protein assay kit Pierce 23225 QIAquick PCR Purification Kit QIAGEN 28106 PureLinkTM HiPure Plasmid Midiprep Kit Invitrogen K210005 Wizard SV Gel and PCR Clean-Up System Promega A9281 the Quick-DNA Microprep Kit Zymo Research D3020 Hoechst 33258 Invitrogen H3569 Deposited data Human Plasma PeptideAtlas Institute for Systems Biology Human Plasma 2025-08 Experimental models: Cell lines .. Neuro-2a ATCC CCL-131; RRID:CVCL_0470 C2C12 Clay F. Semenkovich Lab NA Experimental models: Organisms/strains Rosa26-Cas9 knockin Jackson Laboratory 026179 Rosa26-LSL-Cas9 knockin Jackson Laboratory 026175 Igs2-dCas9-KRAB knockin Jackson Laboratory 030000 Hopx-CreERT2 knockin Jackson Laboratory 017606 Ai14 knockin Jackson Laboratory 007914 Col4a5-G5X mutant Yoav Segal, University of Minnesota NA Pax3-Cre Transgenic Jonathan Epstein, University of Pennsylvania NA Hopx-Cre knockin Jonathan Epstein, University of Pennsylvania NA Nphs2-Cre Transgenic Larry Holzman, University of Pennsylvania NA Lamb2 knockout Jeffrey H. Miner Lab NA Cd2ap knockout Andrey Shaw, Genentech NA Tg: Lama2-gRNA Transgenic This study NA Oligonucleotides Lama2 KO sgRNA #1; ACCCGAGATGTACTGCAAGT This study; IDT NA Lama2 KO sgRNA #2; TACAATATCTATCCCCGCAC This study; IDT NA Lama2 KO sgRNA #3; GGCTGCCTTCACAATTACGT This study; IDT NA Lama2 KO sgRNA #4; GGAACGTTCCCTGGATGACG This study; IDT NA Lama2 KO sgRNA #5; GTGCATTCGAAGCAAGATTC This study; IDT NA Lama2 KO sgRNA #6; AGTTGGTGGAACATGTCCCC This study; IDT NA Lama2 KO sgRNA #7; ATGACGTGGAGTACAAACCC This study; IDT NA Lama2 KO sgRNA #8; CGAGATGTACTGCAAGTTGG This study; IDT NA Lama2 KO sgRNA #9; CTGCTCTTGGGGACGCTCGA This study; IDT NA (Continued on next page) Cell Reports 45, 117006, March 24, 2026 17
Purification:Article Title: Glomerular basement membrane structural integrity dictates trans-tissue deposition of laminin in the kidney.
Article Snippet: Compound Sakura Finetek 4583 4× Laemmli sample buffer Bio-Rad 1610747 2× Laemmli sample buffer Bio-Rad 1610737 CanGet Signal solution 1 TOYOBO NKB-201 CanGet Signal solution 2 TOYOBO NKB-301 ECL Select Western Blotting Detection Reagent Cytiva RPN2235 RIPA buffer containing protease/ phosphatase inhibitor Cell Signaling 5872S Recombinant human laminin-211 protein Peter D. Yurchenco Lab NA Furin inhibitor 1 Calbiochem 344930 (Continued on next page) 16 Cell Reports 45, 117006, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Heparin affinity chromatography (heparin agarose) Sigma H6508 Adriamycin (Doxorubicin hydrochloride) MP Biomedicals 159101 Critical commercial assays BCA protein assay kit Pierce 23225 QIAquick PCR Purification Kit QIAGEN 28106 PureLinkTM HiPure Plasmid Midiprep Kit Invitrogen K210005 Wizard SV Gel and PCR Clean-Up System Promega A9281 the Quick-DNA Microprep Kit Zymo Research D3020 Hoechst 33258 Invitrogen H3569 Deposited data Human Plasma PeptideAtlas Institute for Systems Biology Human Plasma 2025-08 Experimental models: Cell lines .. Neuro-2a ATCC CCL-131; RRID:CVCL_0470 C2C12 Clay F. Semenkovich Lab NA Experimental models: Organisms/strains Rosa26-Cas9 knockin Jackson Laboratory 026179 Rosa26-LSL-Cas9 knockin Jackson Laboratory 026175 Igs2-dCas9-KRAB knockin Jackson Laboratory 030000 Hopx-CreERT2 knockin Jackson Laboratory 017606 Ai14 knockin Jackson Laboratory 007914 Col4a5-G5X mutant Yoav Segal, University of Minnesota NA Pax3-Cre Transgenic Jonathan Epstein, University of Pennsylvania NA Hopx-Cre knockin Jonathan Epstein, University of Pennsylvania NA Nphs2-Cre Transgenic Larry Holzman, University of Pennsylvania NA Lamb2 knockout Jeffrey H. Miner Lab NA Cd2ap knockout Andrey Shaw, Genentech NA Tg: Lama2-gRNA Transgenic This study NA Oligonucleotides Lama2 KO sgRNA #1; ACCCGAGATGTACTGCAAGT This study; IDT NA Lama2 KO sgRNA #2; TACAATATCTATCCCCGCAC This study; IDT NA Lama2 KO sgRNA #3; GGCTGCCTTCACAATTACGT This study; IDT NA Lama2 KO sgRNA #4; GGAACGTTCCCTGGATGACG This study; IDT NA Lama2 KO sgRNA #5; GTGCATTCGAAGCAAGATTC This study; IDT NA Lama2 KO sgRNA #6; AGTTGGTGGAACATGTCCCC This study; IDT NA Lama2 KO sgRNA #7; ATGACGTGGAGTACAAACCC This study; IDT NA Lama2 KO sgRNA #8; CGAGATGTACTGCAAGTTGG This study; IDT NA Lama2 KO sgRNA #9; CTGCTCTTGGGGACGCTCGA This study; IDT NA (Continued on next page) Cell Reports 45, 117006, March 24, 2026 17
Plasmid Preparation:Article Title: Glomerular basement membrane structural integrity dictates trans-tissue deposition of laminin in the kidney.
Article Snippet: Compound Sakura Finetek 4583 4× Laemmli sample buffer Bio-Rad 1610747 2× Laemmli sample buffer Bio-Rad 1610737 CanGet Signal solution 1 TOYOBO NKB-201 CanGet Signal solution 2 TOYOBO NKB-301 ECL Select Western Blotting Detection Reagent Cytiva RPN2235 RIPA buffer containing protease/ phosphatase inhibitor Cell Signaling 5872S Recombinant human laminin-211 protein Peter D. Yurchenco Lab NA Furin inhibitor 1 Calbiochem 344930 (Continued on next page) 16 Cell Reports 45, 117006, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Heparin affinity chromatography (heparin agarose) Sigma H6508 Adriamycin (Doxorubicin hydrochloride) MP Biomedicals 159101 Critical commercial assays BCA protein assay kit Pierce 23225 QIAquick PCR Purification Kit QIAGEN 28106 PureLinkTM HiPure Plasmid Midiprep Kit Invitrogen K210005 Wizard SV Gel and PCR Clean-Up System Promega A9281 the Quick-DNA Microprep Kit Zymo Research D3020 Hoechst 33258 Invitrogen H3569 Deposited data Human Plasma PeptideAtlas Institute for Systems Biology Human Plasma 2025-08 Experimental models: Cell lines .. Neuro-2a ATCC CCL-131; RRID:CVCL_0470 C2C12 Clay F. Semenkovich Lab NA Experimental models: Organisms/strains Rosa26-Cas9 knockin Jackson Laboratory 026179 Rosa26-LSL-Cas9 knockin Jackson Laboratory 026175 Igs2-dCas9-KRAB knockin Jackson Laboratory 030000 Hopx-CreERT2 knockin Jackson Laboratory 017606 Ai14 knockin Jackson Laboratory 007914 Col4a5-G5X mutant Yoav Segal, University of Minnesota NA Pax3-Cre Transgenic Jonathan Epstein, University of Pennsylvania NA Hopx-Cre knockin Jonathan Epstein, University of Pennsylvania NA Nphs2-Cre Transgenic Larry Holzman, University of Pennsylvania NA Lamb2 knockout Jeffrey H. Miner Lab NA Cd2ap knockout Andrey Shaw, Genentech NA Tg: Lama2-gRNA Transgenic This study NA Oligonucleotides Lama2 KO sgRNA #1; ACCCGAGATGTACTGCAAGT This study; IDT NA Lama2 KO sgRNA #2; TACAATATCTATCCCCGCAC This study; IDT NA Lama2 KO sgRNA #3; GGCTGCCTTCACAATTACGT This study; IDT NA Lama2 KO sgRNA #4; GGAACGTTCCCTGGATGACG This study; IDT NA Lama2 KO sgRNA #5; GTGCATTCGAAGCAAGATTC This study; IDT NA Lama2 KO sgRNA #6; AGTTGGTGGAACATGTCCCC This study; IDT NA Lama2 KO sgRNA #7; ATGACGTGGAGTACAAACCC This study; IDT NA Lama2 KO sgRNA #8; CGAGATGTACTGCAAGTTGG This study; IDT NA Lama2 KO sgRNA #9; CTGCTCTTGGGGACGCTCGA This study; IDT NA (Continued on next page) Cell Reports 45, 117006, March 24, 2026 17
Clinical Proteomics:Article Title: Glomerular basement membrane structural integrity dictates trans-tissue deposition of laminin in the kidney.
Article Snippet: Compound Sakura Finetek 4583 4× Laemmli sample buffer Bio-Rad 1610747 2× Laemmli sample buffer Bio-Rad 1610737 CanGet Signal solution 1 TOYOBO NKB-201 CanGet Signal solution 2 TOYOBO NKB-301 ECL Select Western Blotting Detection Reagent Cytiva RPN2235 RIPA buffer containing protease/ phosphatase inhibitor Cell Signaling 5872S Recombinant human laminin-211 protein Peter D. Yurchenco Lab NA Furin inhibitor 1 Calbiochem 344930 (Continued on next page) 16 Cell Reports 45, 117006, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Heparin affinity chromatography (heparin agarose) Sigma H6508 Adriamycin (Doxorubicin hydrochloride) MP Biomedicals 159101 Critical commercial assays BCA protein assay kit Pierce 23225 QIAquick PCR Purification Kit QIAGEN 28106 PureLinkTM HiPure Plasmid Midiprep Kit Invitrogen K210005 Wizard SV Gel and PCR Clean-Up System Promega A9281 the Quick-DNA Microprep Kit Zymo Research D3020 Hoechst 33258 Invitrogen H3569 Deposited data Human Plasma PeptideAtlas Institute for Systems Biology Human Plasma 2025-08 Experimental models: Cell lines .. Neuro-2a ATCC CCL-131; RRID:CVCL_0470 C2C12 Clay F. Semenkovich Lab NA Experimental models: Organisms/strains Rosa26-Cas9 knockin Jackson Laboratory 026179 Rosa26-LSL-Cas9 knockin Jackson Laboratory 026175 Igs2-dCas9-KRAB knockin Jackson Laboratory 030000 Hopx-CreERT2 knockin Jackson Laboratory 017606 Ai14 knockin Jackson Laboratory 007914 Col4a5-G5X mutant Yoav Segal, University of Minnesota NA Pax3-Cre Transgenic Jonathan Epstein, University of Pennsylvania NA Hopx-Cre knockin Jonathan Epstein, University of Pennsylvania NA Nphs2-Cre Transgenic Larry Holzman, University of Pennsylvania NA Lamb2 knockout Jeffrey H. Miner Lab NA Cd2ap knockout Andrey Shaw, Genentech NA Tg: Lama2-gRNA Transgenic This study NA Oligonucleotides Lama2 KO sgRNA #1; ACCCGAGATGTACTGCAAGT This study; IDT NA Lama2 KO sgRNA #2; TACAATATCTATCCCCGCAC This study; IDT NA Lama2 KO sgRNA #3; GGCTGCCTTCACAATTACGT This study; IDT NA Lama2 KO sgRNA #4; GGAACGTTCCCTGGATGACG This study; IDT NA Lama2 KO sgRNA #5; GTGCATTCGAAGCAAGATTC This study; IDT NA Lama2 KO sgRNA #6; AGTTGGTGGAACATGTCCCC This study; IDT NA Lama2 KO sgRNA #7; ATGACGTGGAGTACAAACCC This study; IDT NA Lama2 KO sgRNA #8; CGAGATGTACTGCAAGTTGG This study; IDT NA Lama2 KO sgRNA #9; CTGCTCTTGGGGACGCTCGA This study; IDT NA (Continued on next page) Cell Reports 45, 117006, March 24, 2026 17
other:Article Title: Cross-regulation between FOXC1 and OCT4/SOX2 ensures acquisition of chemoresistance in breast cancer stem cells: Amendment by hsa-miR-5688
Article Snippet: To assess the effect of chemotherapy on cells, mammospheres, and sorted CSCs, they were treated with chemotherapeutic agent Dox (MP Biomedicals) (2.5 μM) for 24 h.36,53 Molecular Therapy: Oncology Vol.
Article Title: hsa-miR-5688 inhibits FOXC1-OCT4/SOX2 feedforward loop that drives chemoresistance in breast cancer stem cells
Article Snippet: To assess the effect of chemotherapy on cells, mammospheres, and sorted CSCs, they were treated with chemotherapeutic agent Dox (MP Biomedicals) (2.5 μM) for 24 h. ,
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