other:Article Title: Necrostatin-1 treatment inhibits osteocyte necroptosis and trabecular deterioration in ovariectomized rats
Article Snippet: Necrostatin-1 (Nec-1, Sigma-Aldrich, USA), Z-VAD-fmk (Z-VAD, MP Biomedicals, Solon, OH, USA) and 17β-estradiol (E2, Sigma Chemical Co. USA) were dissolved in 10% dimethyl sulfoxide (DMSO, Sigma, USA).
Article Title: Loop Diuretics Inhibit Ischemia-Induced Intracellular Ca 2+ Overload in Neurons via the Inhibition of Voltage-Gated Ca 2+ and Na + Channels
Article Snippet: The following reagents were used: TEA (Acros Organics, Waltham, MA); TTX (Alomone Labs, Jerusalem, Israel); Antibiotic/Antimycotic, BSA, DMEM, DMSO, FBS (Fisher Scientific, Fair Lawn, NJ); B-27, Fura-2 AM, Neurobasal (Life Technologies, Carlsbad, CA); Ethacrynic Acid (MP Biomedicals, LLC, Solon, OH); and Bumetanide, EGTA, HEPES, L-Glutamine, Poly-L-Lysine (Sigma-Aldrich, St. Louis, MO).
Article Title: The C-terminus of the B-chain of human insulin-like peptide 5 is critical for cognate RXFP4 receptor activity.
Article Snippet: 2, 2′-dipyridyl disulfide (DPDS) was from Fluka (Bucha, Switzerland) and trifluoromethanesulfonic acid (TFMSA) from MP Biomedicals (Sydney, Australia).
Article Title: Plasminogen Binding and Activation at the Mesothelial Cell Surface Promotes Invasion through a Collagen Matrix
Article Snippet: All reagents were commercially purchased as follows: Glu-Plg (R&D Systems), Lys-Plg (ThermoFisher, Waltham, MA, USA), FastGro synthetic FBS (MP Biomedicals, Solon, OH, USA), bovine serum albumin (BSA, Millipore Sigma, Burlington, MA, USA), UK371804 (Cayman Chemical, Ann Arbor, MI, USA), and ε-aminocaproic acid (EACA, MP Biomedicals, Solon, OH, USA).
Staining:Article Title: Separation and Detection of Phosphorylated and Nonphosphorylated BvgA, a Bordetella pertussis Response Regulator, in vivo and in vitro
Article Snippet: .. Bordetella pertussis strain BP536 is the tet -resistant derivative of the clinical isolate Tohama I strain (laboratory inventory) Formic acid (> 95%) (Sigma-Aldrich, catalog number: F0507) NaOH (Thermo Fisher Scientific, catalog number: SS255-1) Tris base (MP Biomedicals, catalog number: 819620) Bromophenol blue (Sigma-Aldrich, catalog number: B8026) Glycerol (Invitrogen, catalog number: 15514-011) Butanol (Sigma-Aldrich, catalog number: 15467-9) 30% 29:1 Acrylamide/bis-acrylamide (Sigma-Aldrich, catalog number: A3574) 10% SDS (Hoefer, catalog number: GR155-1) Phos-tagTM acrylamide (Wako Pure Chemical Industries, catalog number: AAL-107) Zn(NO 3 ) 2 (Sigma-Aldrich, catalog number: 228737) Ammonium persulfate (Sigma-Aldrich, catalog number: 3678) N,N,N’,N’-Tetramethylethylenediamine (TEMED) (Sigma-Aldrich, catalog number: T9281) 0.5 M EDTA, pH 8.0 (ResGen, catalog number: 750009) MOPS (Fluka, catalog number: 69947) Sodium metabisulfite (Na 2 S 2 O 5 ) (Sigma-Aldrich, catalog number: S9000) Glycine (Sigma-Aldrich, catalog number: G7126-1KG) Methanol (EMD Millipore, catalog number: MX0485-7) PBS buffer (Gibco, catalog number: 2014-10) BactoTM proteose peptone (Becton, Dickinson and Company, catalog number: 211684) DifcoTM Bordet Gengou agar base (Becton, Dickinson and Company, catalog number: 248200) Defibrinated sheep blood (LAMPIRE ® Biological Labs, catalog number: 7239001) Lithium potassium acetyl phosphate (Sigma-Aldrich, catalog number: A0262-500MG) Colloidal Blue staining kit (Life Technologies, catalog number: LC6025) Tween-20 (Bio-Rad Laboratories, catalog number: M3524) Non-fat dry milk (Giant Food Store, catalog number: 688267078330) Monoclonal anti-BvgA antibody (laboratory inventory) HRP-conjugated Secondary Antibody (Santa Cruz, catalog number: SC2005) Amersham ECL Primer Western Blotting Detection System (General Electric Company, catalog number: RPN2232) PVDF filter (Invitrogen, catalog number: LC6025) BSA (Albumin from bovine serum, Sigma-Aldrich, catalog number: A2153) 1 M formic acid (see Recipes) 5 N NaOH (see Recipes) 1 M Tris-Cl solutions (see Recipes) 1% Bromophenol blue (see Recipes) 5x Loading Solution (see Recipes) Water-saturated butanol (see Recipes) 5 mM Phos-tagTM acrylamide (see Recipes) 10 mM Zn(NO 3 ) 2 (see Recipes) 10% APS (see Recipes) 1x MOPS Running Buffer, pH 7.8 (see Recipes) Transfer Buffer (see Recipes) BG agar plates (see Recipes) 200 mM acetyl phosphate (see Recipes) 1% or 5% BSA in PBS (see Recipes) 0.05% Tween-20 in PBS (see Recipes) 1% Non-fat milk in PBS (see Recipes) .. Polyester-tipped applicator (Puritan Medical, catalog number: 25-806 1PD) Spectrophotometer Mini gel cassettes (1.0 mm) (Invitrogen, catalog number: NC2010) 10, 12 or 15-well combs (1.0 mm) (Invitrogen, catalog number: NC3015) XCell SureLock ® Mini-Cell (Invitrogen, catalog number: EI0001) Platform shaker Eppendorf microfuge Bio-Rad Mini-Protean II (Bio-Rad Laboratories)
Food & Beverages:Article Title: Separation and Detection of Phosphorylated and Nonphosphorylated BvgA, a Bordetella pertussis Response Regulator, in vivo and in vitro
Article Snippet: .. Bordetella pertussis strain BP536 is the tet -resistant derivative of the clinical isolate Tohama I strain (laboratory inventory) Formic acid (> 95%) (Sigma-Aldrich, catalog number: F0507) NaOH (Thermo Fisher Scientific, catalog number: SS255-1) Tris base (MP Biomedicals, catalog number: 819620) Bromophenol blue (Sigma-Aldrich, catalog number: B8026) Glycerol (Invitrogen, catalog number: 15514-011) Butanol (Sigma-Aldrich, catalog number: 15467-9) 30% 29:1 Acrylamide/bis-acrylamide (Sigma-Aldrich, catalog number: A3574) 10% SDS (Hoefer, catalog number: GR155-1) Phos-tagTM acrylamide (Wako Pure Chemical Industries, catalog number: AAL-107) Zn(NO 3 ) 2 (Sigma-Aldrich, catalog number: 228737) Ammonium persulfate (Sigma-Aldrich, catalog number: 3678) N,N,N’,N’-Tetramethylethylenediamine (TEMED) (Sigma-Aldrich, catalog number: T9281) 0.5 M EDTA, pH 8.0 (ResGen, catalog number: 750009) MOPS (Fluka, catalog number: 69947) Sodium metabisulfite (Na 2 S 2 O 5 ) (Sigma-Aldrich, catalog number: S9000) Glycine (Sigma-Aldrich, catalog number: G7126-1KG) Methanol (EMD Millipore, catalog number: MX0485-7) PBS buffer (Gibco, catalog number: 2014-10) BactoTM proteose peptone (Becton, Dickinson and Company, catalog number: 211684) DifcoTM Bordet Gengou agar base (Becton, Dickinson and Company, catalog number: 248200) Defibrinated sheep blood (LAMPIRE ® Biological Labs, catalog number: 7239001) Lithium potassium acetyl phosphate (Sigma-Aldrich, catalog number: A0262-500MG) Colloidal Blue staining kit (Life Technologies, catalog number: LC6025) Tween-20 (Bio-Rad Laboratories, catalog number: M3524) Non-fat dry milk (Giant Food Store, catalog number: 688267078330) Monoclonal anti-BvgA antibody (laboratory inventory) HRP-conjugated Secondary Antibody (Santa Cruz, catalog number: SC2005) Amersham ECL Primer Western Blotting Detection System (General Electric Company, catalog number: RPN2232) PVDF filter (Invitrogen, catalog number: LC6025) BSA (Albumin from bovine serum, Sigma-Aldrich, catalog number: A2153) 1 M formic acid (see Recipes) 5 N NaOH (see Recipes) 1 M Tris-Cl solutions (see Recipes) 1% Bromophenol blue (see Recipes) 5x Loading Solution (see Recipes) Water-saturated butanol (see Recipes) 5 mM Phos-tagTM acrylamide (see Recipes) 10 mM Zn(NO 3 ) 2 (see Recipes) 10% APS (see Recipes) 1x MOPS Running Buffer, pH 7.8 (see Recipes) Transfer Buffer (see Recipes) BG agar plates (see Recipes) 200 mM acetyl phosphate (see Recipes) 1% or 5% BSA in PBS (see Recipes) 0.05% Tween-20 in PBS (see Recipes) 1% Non-fat milk in PBS (see Recipes) .. Polyester-tipped applicator (Puritan Medical, catalog number: 25-806 1PD) Spectrophotometer Mini gel cassettes (1.0 mm) (Invitrogen, catalog number: NC2010) 10, 12 or 15-well combs (1.0 mm) (Invitrogen, catalog number: NC3015) XCell SureLock ® Mini-Cell (Invitrogen, catalog number: EI0001) Platform shaker Eppendorf microfuge Bio-Rad Mini-Protean II (Bio-Rad Laboratories)
Western Blot:Article Title: Separation and Detection of Phosphorylated and Nonphosphorylated BvgA, a Bordetella pertussis Response Regulator, in vivo and in vitro
Article Snippet: .. Bordetella pertussis strain BP536 is the tet -resistant derivative of the clinical isolate Tohama I strain (laboratory inventory) Formic acid (> 95%) (Sigma-Aldrich, catalog number: F0507) NaOH (Thermo Fisher Scientific, catalog number: SS255-1) Tris base (MP Biomedicals, catalog number: 819620) Bromophenol blue (Sigma-Aldrich, catalog number: B8026) Glycerol (Invitrogen, catalog number: 15514-011) Butanol (Sigma-Aldrich, catalog number: 15467-9) 30% 29:1 Acrylamide/bis-acrylamide (Sigma-Aldrich, catalog number: A3574) 10% SDS (Hoefer, catalog number: GR155-1) Phos-tagTM acrylamide (Wako Pure Chemical Industries, catalog number: AAL-107) Zn(NO 3 ) 2 (Sigma-Aldrich, catalog number: 228737) Ammonium persulfate (Sigma-Aldrich, catalog number: 3678) N,N,N’,N’-Tetramethylethylenediamine (TEMED) (Sigma-Aldrich, catalog number: T9281) 0.5 M EDTA, pH 8.0 (ResGen, catalog number: 750009) MOPS (Fluka, catalog number: 69947) Sodium metabisulfite (Na 2 S 2 O 5 ) (Sigma-Aldrich, catalog number: S9000) Glycine (Sigma-Aldrich, catalog number: G7126-1KG) Methanol (EMD Millipore, catalog number: MX0485-7) PBS buffer (Gibco, catalog number: 2014-10) BactoTM proteose peptone (Becton, Dickinson and Company, catalog number: 211684) DifcoTM Bordet Gengou agar base (Becton, Dickinson and Company, catalog number: 248200) Defibrinated sheep blood (LAMPIRE ® Biological Labs, catalog number: 7239001) Lithium potassium acetyl phosphate (Sigma-Aldrich, catalog number: A0262-500MG) Colloidal Blue staining kit (Life Technologies, catalog number: LC6025) Tween-20 (Bio-Rad Laboratories, catalog number: M3524) Non-fat dry milk (Giant Food Store, catalog number: 688267078330) Monoclonal anti-BvgA antibody (laboratory inventory) HRP-conjugated Secondary Antibody (Santa Cruz, catalog number: SC2005) Amersham ECL Primer Western Blotting Detection System (General Electric Company, catalog number: RPN2232) PVDF filter (Invitrogen, catalog number: LC6025) BSA (Albumin from bovine serum, Sigma-Aldrich, catalog number: A2153) 1 M formic acid (see Recipes) 5 N NaOH (see Recipes) 1 M Tris-Cl solutions (see Recipes) 1% Bromophenol blue (see Recipes) 5x Loading Solution (see Recipes) Water-saturated butanol (see Recipes) 5 mM Phos-tagTM acrylamide (see Recipes) 10 mM Zn(NO 3 ) 2 (see Recipes) 10% APS (see Recipes) 1x MOPS Running Buffer, pH 7.8 (see Recipes) Transfer Buffer (see Recipes) BG agar plates (see Recipes) 200 mM acetyl phosphate (see Recipes) 1% or 5% BSA in PBS (see Recipes) 0.05% Tween-20 in PBS (see Recipes) 1% Non-fat milk in PBS (see Recipes) .. Polyester-tipped applicator (Puritan Medical, catalog number: 25-806 1PD) Spectrophotometer Mini gel cassettes (1.0 mm) (Invitrogen, catalog number: NC2010) 10, 12 or 15-well combs (1.0 mm) (Invitrogen, catalog number: NC3015) XCell SureLock ® Mini-Cell (Invitrogen, catalog number: EI0001) Platform shaker Eppendorf microfuge Bio-Rad Mini-Protean II (Bio-Rad Laboratories)
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