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roseoflavin  (Valiant Co Ltd)

 
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    Valiant Co Ltd roseoflavin
    Roseoflavin, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/0215425210/Roseoflavin/10__1128_slash_mbio__00526___17-191-10-21
    Average 90 stars, based on 10 article reviews
    roseoflavin - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: Characterization of the small flavin-binding dodecin in the roseoflavin producer Streptomyces davawensis.
    Article Snippet: Roseoflavin was obtained from MP Biomedicals (Heidelberg, Germany).

    Article Title: Dual-Targeting Small-Molecule Inhibitors of the Staphylococcus aureus FMN Riboswitch Disrupt Riboflavin Homeostasis in an Infectious Setting.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 1920, E. faecalis, FAD binding enzyme(EF1586) Fwd seq: CGTAGTCG TAGGATGTACCCA This paper NA 1921, E. faecalis, FAD binding enzyme (EF1586) Rev seq: GCGGTCT AACCCGTCAACT This paper NA 1922, E. faecalis, intergnic (EF2734-2735) Fwd seq: CCAAGTGGC GGCATTCACT This paper NA 1923, E. faecalis, intergnic (EF2734-2735) Rev seq: GCTGTGCCAGAA TAGCCT This paper NA Recombinant DNA pnuX from C. glutamicum cloned into pET29 using Nde1& BamH1 This paper NA DribD constructed from S. aureus using 2-way PCR and cloned into pSAKOts This paper; vector from Mann et al., 2016 NA Software and Algorithms Sequencher 5.0 Gene Codes Version 5.0 Vector NTI Advance Invitrogen 11.5.1 Prism 7.02 Graph Pad graphpad.com/ program mutate_bases 3DNA x3dna.org/ SeqPrep https://github.com/ jstjohn/SeqPrep BWA algorithm Li and Durbin, 2009 biobwa.sourceforge.net/ bwa.shtml GATK (Genome Analysis Toolkit) Broad Institute software.broadinstitute.org/gatk/

    Article Title: The roseoflavin producer Streptomyces davaonensis has a high catalytic capacity and specific genetic adaptations with regard to the biosynthesis of riboflavin.
    Article Snippet: The bacterium Streptomyces davaonensis synthesizes the antibiotic roseoflavin in the stationary phase of growth.. The starting point for roseoflavin biosynthesis is riboflavin (vitamin B2) and four enzymes (RibC, RosB, RosA and RosC) are necessary to convert a vitamin (riboflavin) into a potent, broad-spectrum antibiotic (roseoflavin).. In S. davaonensis seven enzymatic functions are required to synthesize the roseoflavin precursor riboflavin from the central building blocks GTP and ribulose 5-phosphate.

    Binding Assay:

    Article Title: Characterization of the small flavin-binding dodecin in the roseoflavin producer Streptomyces davawensis.
    Article Snippet: Roseoflavin was obtained from MP Biomedicals (Heidelberg, Germany).

    Article Title: Dual-Targeting Small-Molecule Inhibitors of the Staphylococcus aureus FMN Riboswitch Disrupt Riboflavin Homeostasis in an Infectious Setting.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 1920, E. faecalis, FAD binding enzyme(EF1586) Fwd seq: CGTAGTCG TAGGATGTACCCA This paper NA 1921, E. faecalis, FAD binding enzyme (EF1586) Rev seq: GCGGTCT AACCCGTCAACT This paper NA 1922, E. faecalis, intergnic (EF2734-2735) Fwd seq: CCAAGTGGC GGCATTCACT This paper NA 1923, E. faecalis, intergnic (EF2734-2735) Rev seq: GCTGTGCCAGAA TAGCCT This paper NA Recombinant DNA pnuX from C. glutamicum cloned into pET29 using Nde1& BamH1 This paper NA DribD constructed from S. aureus using 2-way PCR and cloned into pSAKOts This paper; vector from Mann et al., 2016 NA Software and Algorithms Sequencher 5.0 Gene Codes Version 5.0 Vector NTI Advance Invitrogen 11.5.1 Prism 7.02 Graph Pad graphpad.com/ program mutate_bases 3DNA x3dna.org/ SeqPrep https://github.com/ jstjohn/SeqPrep BWA algorithm Li and Durbin, 2009 biobwa.sourceforge.net/ bwa.shtml GATK (Genome Analysis Toolkit) Broad Institute software.broadinstitute.org/gatk/

    Article Title: The roseoflavin producer Streptomyces davaonensis has a high catalytic capacity and specific genetic adaptations with regard to the biosynthesis of riboflavin.
    Article Snippet: The bacterium Streptomyces davaonensis synthesizes the antibiotic roseoflavin in the stationary phase of growth.. The starting point for roseoflavin biosynthesis is riboflavin (vitamin B2) and four enzymes (RibC, RosB, RosA and RosC) are necessary to convert a vitamin (riboflavin) into a potent, broad-spectrum antibiotic (roseoflavin).. In S. davaonensis seven enzymatic functions are required to synthesize the roseoflavin precursor riboflavin from the central building blocks GTP and ribulose 5-phosphate.

    Recombinant:

    Article Title: Characterization of the small flavin-binding dodecin in the roseoflavin producer Streptomyces davawensis.
    Article Snippet: Roseoflavin was obtained from MP Biomedicals (Heidelberg, Germany).

    Article Title: Dual-Targeting Small-Molecule Inhibitors of the Staphylococcus aureus FMN Riboswitch Disrupt Riboflavin Homeostasis in an Infectious Setting.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 1920, E. faecalis, FAD binding enzyme(EF1586) Fwd seq: CGTAGTCG TAGGATGTACCCA This paper NA 1921, E. faecalis, FAD binding enzyme (EF1586) Rev seq: GCGGTCT AACCCGTCAACT This paper NA 1922, E. faecalis, intergnic (EF2734-2735) Fwd seq: CCAAGTGGC GGCATTCACT This paper NA 1923, E. faecalis, intergnic (EF2734-2735) Rev seq: GCTGTGCCAGAA TAGCCT This paper NA Recombinant DNA pnuX from C. glutamicum cloned into pET29 using Nde1& BamH1 This paper NA DribD constructed from S. aureus using 2-way PCR and cloned into pSAKOts This paper; vector from Mann et al., 2016 NA Software and Algorithms Sequencher 5.0 Gene Codes Version 5.0 Vector NTI Advance Invitrogen 11.5.1 Prism 7.02 Graph Pad graphpad.com/ program mutate_bases 3DNA x3dna.org/ SeqPrep https://github.com/ jstjohn/SeqPrep BWA algorithm Li and Durbin, 2009 biobwa.sourceforge.net/ bwa.shtml GATK (Genome Analysis Toolkit) Broad Institute software.broadinstitute.org/gatk/

    Article Title: The roseoflavin producer Streptomyces davaonensis has a high catalytic capacity and specific genetic adaptations with regard to the biosynthesis of riboflavin.
    Article Snippet: The bacterium Streptomyces davaonensis synthesizes the antibiotic roseoflavin in the stationary phase of growth.. The starting point for roseoflavin biosynthesis is riboflavin (vitamin B2) and four enzymes (RibC, RosB, RosA and RosC) are necessary to convert a vitamin (riboflavin) into a potent, broad-spectrum antibiotic (roseoflavin).. In S. davaonensis seven enzymatic functions are required to synthesize the roseoflavin precursor riboflavin from the central building blocks GTP and ribulose 5-phosphate.

    High Performance Liquid Chromatography:

    Article Title: Characterization of the small flavin-binding dodecin in the roseoflavin producer Streptomyces davawensis.
    Article Snippet: Roseoflavin was obtained from MP Biomedicals (Heidelberg, Germany).

    Article Title: Dual-Targeting Small-Molecule Inhibitors of the Staphylococcus aureus FMN Riboswitch Disrupt Riboflavin Homeostasis in an Infectious Setting.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 1920, E. faecalis, FAD binding enzyme(EF1586) Fwd seq: CGTAGTCG TAGGATGTACCCA This paper NA 1921, E. faecalis, FAD binding enzyme (EF1586) Rev seq: GCGGTCT AACCCGTCAACT This paper NA 1922, E. faecalis, intergnic (EF2734-2735) Fwd seq: CCAAGTGGC GGCATTCACT This paper NA 1923, E. faecalis, intergnic (EF2734-2735) Rev seq: GCTGTGCCAGAA TAGCCT This paper NA Recombinant DNA pnuX from C. glutamicum cloned into pET29 using Nde1& BamH1 This paper NA DribD constructed from S. aureus using 2-way PCR and cloned into pSAKOts This paper; vector from Mann et al., 2016 NA Software and Algorithms Sequencher 5.0 Gene Codes Version 5.0 Vector NTI Advance Invitrogen 11.5.1 Prism 7.02 Graph Pad graphpad.com/ program mutate_bases 3DNA x3dna.org/ SeqPrep https://github.com/ jstjohn/SeqPrep BWA algorithm Li and Durbin, 2009 biobwa.sourceforge.net/ bwa.shtml GATK (Genome Analysis Toolkit) Broad Institute software.broadinstitute.org/gatk/

    Article Title: The roseoflavin producer Streptomyces davaonensis has a high catalytic capacity and specific genetic adaptations with regard to the biosynthesis of riboflavin.
    Article Snippet: The bacterium Streptomyces davaonensis synthesizes the antibiotic roseoflavin in the stationary phase of growth.. The starting point for roseoflavin biosynthesis is riboflavin (vitamin B2) and four enzymes (RibC, RosB, RosA and RosC) are necessary to convert a vitamin (riboflavin) into a potent, broad-spectrum antibiotic (roseoflavin).. In S. davaonensis seven enzymatic functions are required to synthesize the roseoflavin precursor riboflavin from the central building blocks GTP and ribulose 5-phosphate.

    Sequencing:

    Article Title: Characterization of the small flavin-binding dodecin in the roseoflavin producer Streptomyces davawensis.
    Article Snippet: Roseoflavin was obtained from MP Biomedicals (Heidelberg, Germany).

    Article Title: Dual-Targeting Small-Molecule Inhibitors of the Staphylococcus aureus FMN Riboswitch Disrupt Riboflavin Homeostasis in an Infectious Setting.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 1920, E. faecalis, FAD binding enzyme(EF1586) Fwd seq: CGTAGTCG TAGGATGTACCCA This paper NA 1921, E. faecalis, FAD binding enzyme (EF1586) Rev seq: GCGGTCT AACCCGTCAACT This paper NA 1922, E. faecalis, intergnic (EF2734-2735) Fwd seq: CCAAGTGGC GGCATTCACT This paper NA 1923, E. faecalis, intergnic (EF2734-2735) Rev seq: GCTGTGCCAGAA TAGCCT This paper NA Recombinant DNA pnuX from C. glutamicum cloned into pET29 using Nde1& BamH1 This paper NA DribD constructed from S. aureus using 2-way PCR and cloned into pSAKOts This paper; vector from Mann et al., 2016 NA Software and Algorithms Sequencher 5.0 Gene Codes Version 5.0 Vector NTI Advance Invitrogen 11.5.1 Prism 7.02 Graph Pad graphpad.com/ program mutate_bases 3DNA x3dna.org/ SeqPrep https://github.com/ jstjohn/SeqPrep BWA algorithm Li and Durbin, 2009 biobwa.sourceforge.net/ bwa.shtml GATK (Genome Analysis Toolkit) Broad Institute software.broadinstitute.org/gatk/

    Article Title: The roseoflavin producer Streptomyces davaonensis has a high catalytic capacity and specific genetic adaptations with regard to the biosynthesis of riboflavin.
    Article Snippet: The bacterium Streptomyces davaonensis synthesizes the antibiotic roseoflavin in the stationary phase of growth.. The starting point for roseoflavin biosynthesis is riboflavin (vitamin B2) and four enzymes (RibC, RosB, RosA and RosC) are necessary to convert a vitamin (riboflavin) into a potent, broad-spectrum antibiotic (roseoflavin).. In S. davaonensis seven enzymatic functions are required to synthesize the roseoflavin precursor riboflavin from the central building blocks GTP and ribulose 5-phosphate.

    Next-Generation Sequencing:

    Article Title: Characterization of the small flavin-binding dodecin in the roseoflavin producer Streptomyces davawensis.
    Article Snippet: Roseoflavin was obtained from MP Biomedicals (Heidelberg, Germany).

    Article Title: Dual-Targeting Small-Molecule Inhibitors of the Staphylococcus aureus FMN Riboswitch Disrupt Riboflavin Homeostasis in an Infectious Setting.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 1920, E. faecalis, FAD binding enzyme(EF1586) Fwd seq: CGTAGTCG TAGGATGTACCCA This paper NA 1921, E. faecalis, FAD binding enzyme (EF1586) Rev seq: GCGGTCT AACCCGTCAACT This paper NA 1922, E. faecalis, intergnic (EF2734-2735) Fwd seq: CCAAGTGGC GGCATTCACT This paper NA 1923, E. faecalis, intergnic (EF2734-2735) Rev seq: GCTGTGCCAGAA TAGCCT This paper NA Recombinant DNA pnuX from C. glutamicum cloned into pET29 using Nde1& BamH1 This paper NA DribD constructed from S. aureus using 2-way PCR and cloned into pSAKOts This paper; vector from Mann et al., 2016 NA Software and Algorithms Sequencher 5.0 Gene Codes Version 5.0 Vector NTI Advance Invitrogen 11.5.1 Prism 7.02 Graph Pad graphpad.com/ program mutate_bases 3DNA x3dna.org/ SeqPrep https://github.com/ jstjohn/SeqPrep BWA algorithm Li and Durbin, 2009 biobwa.sourceforge.net/ bwa.shtml GATK (Genome Analysis Toolkit) Broad Institute software.broadinstitute.org/gatk/

    Article Title: The roseoflavin producer Streptomyces davaonensis has a high catalytic capacity and specific genetic adaptations with regard to the biosynthesis of riboflavin.
    Article Snippet: The bacterium Streptomyces davaonensis synthesizes the antibiotic roseoflavin in the stationary phase of growth.. The starting point for roseoflavin biosynthesis is riboflavin (vitamin B2) and four enzymes (RibC, RosB, RosA and RosC) are necessary to convert a vitamin (riboflavin) into a potent, broad-spectrum antibiotic (roseoflavin).. In S. davaonensis seven enzymatic functions are required to synthesize the roseoflavin precursor riboflavin from the central building blocks GTP and ribulose 5-phosphate.

    Clone Assay:

    Article Title: Characterization of the small flavin-binding dodecin in the roseoflavin producer Streptomyces davawensis.
    Article Snippet: Roseoflavin was obtained from MP Biomedicals (Heidelberg, Germany).

    Article Title: Dual-Targeting Small-Molecule Inhibitors of the Staphylococcus aureus FMN Riboswitch Disrupt Riboflavin Homeostasis in an Infectious Setting.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 1920, E. faecalis, FAD binding enzyme(EF1586) Fwd seq: CGTAGTCG TAGGATGTACCCA This paper NA 1921, E. faecalis, FAD binding enzyme (EF1586) Rev seq: GCGGTCT AACCCGTCAACT This paper NA 1922, E. faecalis, intergnic (EF2734-2735) Fwd seq: CCAAGTGGC GGCATTCACT This paper NA 1923, E. faecalis, intergnic (EF2734-2735) Rev seq: GCTGTGCCAGAA TAGCCT This paper NA Recombinant DNA pnuX from C. glutamicum cloned into pET29 using Nde1& BamH1 This paper NA DribD constructed from S. aureus using 2-way PCR and cloned into pSAKOts This paper; vector from Mann et al., 2016 NA Software and Algorithms Sequencher 5.0 Gene Codes Version 5.0 Vector NTI Advance Invitrogen 11.5.1 Prism 7.02 Graph Pad graphpad.com/ program mutate_bases 3DNA x3dna.org/ SeqPrep https://github.com/ jstjohn/SeqPrep BWA algorithm Li and Durbin, 2009 biobwa.sourceforge.net/ bwa.shtml GATK (Genome Analysis Toolkit) Broad Institute software.broadinstitute.org/gatk/

    Article Title: The roseoflavin producer Streptomyces davaonensis has a high catalytic capacity and specific genetic adaptations with regard to the biosynthesis of riboflavin.
    Article Snippet: The bacterium Streptomyces davaonensis synthesizes the antibiotic roseoflavin in the stationary phase of growth.. The starting point for roseoflavin biosynthesis is riboflavin (vitamin B2) and four enzymes (RibC, RosB, RosA and RosC) are necessary to convert a vitamin (riboflavin) into a potent, broad-spectrum antibiotic (roseoflavin).. In S. davaonensis seven enzymatic functions are required to synthesize the roseoflavin precursor riboflavin from the central building blocks GTP and ribulose 5-phosphate.



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    Figure 1. FMN Riboswitch Regulation, Cognate Inhibitors, and RF Homeostasis Gene Organization (A) Schematic structure of the FMN riboswitch containing the 50 mRNA FMN aptamer that binds FMN and the 30 expression platform (blue) that directly co- ordinates expression of the downstream rib open reading frame. Left, FMN aptamer binding to the FMN ligand transduces a conformational change in the expression platform (OFF), resulting in the formation of a terminator/sequester loop that abolishes rib expression by sequestering the RBS to prevent translation of completely transcribed rib mRNAs. Right, when FMN levels are low, the FMN aptamer lacking the bound ligand adopts a second structural conformation (ON) that induces an anti-terminator/anti-sequester loop, thereby facilitating uninterrupted rib expression. (B) Chemical structure of ribocil-A, ribocil-B, ribocil-C, FMN, FMNH2, and roseoflavin. (C) Gene organization of MRSA COL RF biosynthetic operon and ribU transporter, with relative genome coordinates from GB accession CP000046.1. FMN riboswitches SA1 and SA2 regulate the RF biosynthetic operon and ribU RF transporter, respectively. See also Figure S1.
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    Image Search Results


    Figure 1. FMN Riboswitch Regulation, Cognate Inhibitors, and RF Homeostasis Gene Organization (A) Schematic structure of the FMN riboswitch containing the 50 mRNA FMN aptamer that binds FMN and the 30 expression platform (blue) that directly co- ordinates expression of the downstream rib open reading frame. Left, FMN aptamer binding to the FMN ligand transduces a conformational change in the expression platform (OFF), resulting in the formation of a terminator/sequester loop that abolishes rib expression by sequestering the RBS to prevent translation of completely transcribed rib mRNAs. Right, when FMN levels are low, the FMN aptamer lacking the bound ligand adopts a second structural conformation (ON) that induces an anti-terminator/anti-sequester loop, thereby facilitating uninterrupted rib expression. (B) Chemical structure of ribocil-A, ribocil-B, ribocil-C, FMN, FMNH2, and roseoflavin. (C) Gene organization of MRSA COL RF biosynthetic operon and ribU transporter, with relative genome coordinates from GB accession CP000046.1. FMN riboswitches SA1 and SA2 regulate the RF biosynthetic operon and ribU RF transporter, respectively. See also Figure S1.

    Journal: Cell chemical biology

    Article Title: Dual-Targeting Small-Molecule Inhibitors of the Staphylococcus aureus FMN Riboswitch Disrupt Riboflavin Homeostasis in an Infectious Setting.

    doi: 10.1016/j.chembiol.2017.03.014

    Figure Lengend Snippet: Figure 1. FMN Riboswitch Regulation, Cognate Inhibitors, and RF Homeostasis Gene Organization (A) Schematic structure of the FMN riboswitch containing the 50 mRNA FMN aptamer that binds FMN and the 30 expression platform (blue) that directly co- ordinates expression of the downstream rib open reading frame. Left, FMN aptamer binding to the FMN ligand transduces a conformational change in the expression platform (OFF), resulting in the formation of a terminator/sequester loop that abolishes rib expression by sequestering the RBS to prevent translation of completely transcribed rib mRNAs. Right, when FMN levels are low, the FMN aptamer lacking the bound ligand adopts a second structural conformation (ON) that induces an anti-terminator/anti-sequester loop, thereby facilitating uninterrupted rib expression. (B) Chemical structure of ribocil-A, ribocil-B, ribocil-C, FMN, FMNH2, and roseoflavin. (C) Gene organization of MRSA COL RF biosynthetic operon and ribU transporter, with relative genome coordinates from GB accession CP000046.1. FMN riboswitches SA1 and SA2 regulate the RF biosynthetic operon and ribU RF transporter, respectively. See also Figure S1.

    Article Snippet: MRSACOLwas grown to late-exponential phase (OD600 1.0; approximately 5X109 CFU/ml) in CAMHB and 100 ml of this broth was spread on CAMH agar plates containing 2-fold escalating agar MIC levels of roseoflavin (RoF) (MP Biomed).

    Techniques: Expressing, Binding Assay