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il 2  (Valiant Co Ltd)

 
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    Valiant Co Ltd il 2
    Il 2, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/0215389610/Interleukin+2/pm21372257-64-6-30
    Average 93 stars, based on 28 article reviews
    il 2 - by Bioz Stars, 2026-09
    93/100 stars

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    Molecular Weight:

    Article Title: Dynamic Changes in Gut Microbiome of Ulcerative Colitis: Initial Study from Animal Model
    Article Snippet: .. The following reagents and instruments were used: DSS (molecular weight = 36 kDa to 50 kDa; MP Biomedical, California, USA) and enzyme-linked immunosorbent assay (ELISA) kits: interleukin-2 (IL-2), interleukin-4 (IL-4), interleukin-6 (IL-6), interleukin-10 (IL-10), interleukin-22 (IL-22), interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), 5-hydroxytryptamine (5-HT) (Neobioscience Technology Company, Shanghai, China). .. Twenty-four pathogen-free healthy male C57BL/6N mice (6 weeks old, 20±2 g) were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd (license number: SCXK (Beijing) 2016-0006) and were maintained at the Animal Experimental Center of Shandong University of Traditional Chinese Medicine (license number: SYXK (Lu) 2017-0022, Shandong, China).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Dynamic Changes in Gut Microbiome of Ulcerative Colitis: Initial Study from Animal Model
    Article Snippet: .. The following reagents and instruments were used: DSS (molecular weight = 36 kDa to 50 kDa; MP Biomedical, California, USA) and enzyme-linked immunosorbent assay (ELISA) kits: interleukin-2 (IL-2), interleukin-4 (IL-4), interleukin-6 (IL-6), interleukin-10 (IL-10), interleukin-22 (IL-22), interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), 5-hydroxytryptamine (5-HT) (Neobioscience Technology Company, Shanghai, China). .. Twenty-four pathogen-free healthy male C57BL/6N mice (6 weeks old, 20±2 g) were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd (license number: SCXK (Beijing) 2016-0006) and were maintained at the Animal Experimental Center of Shandong University of Traditional Chinese Medicine (license number: SYXK (Lu) 2017-0022, Shandong, China).

    Isolation:

    Article Title: Laboratory safety evaluation of lokivetmab, a canine anti-interleukin-31 monoclonal antibody, in dogs.
    Article Snippet: .. PBMCs were isolated by density gradient (LSM, MP Biomedicals) and cryopreserved (− 70 C) until used in IL-2 ELISpot (Venegas-Vargas et al., 2021) and lymphoproliferation assays. ..

    Enzyme-linked Immunospot:

    Article Title: Laboratory safety evaluation of lokivetmab, a canine anti-interleukin-31 monoclonal antibody, in dogs.
    Article Snippet: .. PBMCs were isolated by density gradient (LSM, MP Biomedicals) and cryopreserved (− 70 C) until used in IL-2 ELISpot (Venegas-Vargas et al., 2021) and lymphoproliferation assays. ..

    Derivative Assay:

    Article Title: Neoantigen-targeted dendritic cell vaccination in lung cancer patients induces long-lived T cells exhibiting the full differentiation spectrum.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD3 PE-Cy7 Biolegend Cat # 300420; RRID: AB_439781 CD3 BV421 Biolegend Cat # 317343; RRID: AB_2565848 CD3 BV650 Biolegend Cat # 317323; RRID: AB_11126748 CD4 PerCP/Cy5.5 Biolegend Cat # 344608; RRID: AB_1953236 CD8 APC/Fire750 Biolegend Cat # 344746; RRID: AB_2572095 CD8 PerCP/Cy5.5 Biolegend Cat # 344709; RRID: AB_2044009 CD45 FITC This manuscript N/A CD95 PE/Dazzle594 Biolegend Cat # 305633; RRID: AB_2564220 CD127 BV711 Biolegend Cat # 351327; RRID: AB_11219191 CD137 PE Biolegend Cat # 309804; RRID: AB_314783 IFNg FITC Biolegend Cat # 502506; RRID: AB_315231 CX3CR1 BV421 BD Biosciences Cat # 565801; RRID: AB_2744471 CCR7 BV711 Biolegend Cat # 353228; RRID: AB_2563865 CCR7 APC/Fire750 Biolegend Cat # 353263; RRID: AB_2894483 Granzyme B PE-Cy7 Biolegend Cat # 372213; RRID: AB_2728380 W6/32 HB-95 (Hybridoma) N/A Biological samples Patient PBMC This manuscript N/A Patient tumor samples This manuscript N/A Patient PHA blasts This manuscript N/A PBMC derived from healthy blood donor Rode Kruis Vlaanderen N/A Chemicals, peptides, and recombinant proteins IL-7 Miltenyi Cat # 130-095-367 IL-15 Miltenyi Cat # 130-095-760 Neoantigen peptides for stimulation assays (crude) JPT Custom, PepTrack peptide library Neoantigen peptides for MHC class I tetramer generation (>80% pure) GenScript Custom Peptide-MHC class I tetramers NIH Tetramer Core facility N/A Dasatinib Stemcell Technologies Cat # 73082 Phytohemagglutinin Roche Cat # 11249738001 IL-2 Roche Cat # 11011456001 Nivolumab Bristol Myers Squibb N/A Tris MP Biomedicals Cat # 0210313305 Sodium tetraborate decahydrate Sigma-Aldrich, Merck Cat #B3545-500G Dimethyl pimelimidate dihydrochloride Sigma-Aldrich, Merck Cat #D8388-250MG Ethanolamine Thermo Fisher Scientific Cat # 149582500 Octyl b-D-glucopyranoside Sigma-Aldrich, Merck Cat #O9882-500MG Sodium deoxycholate Milipore, Merck Cat # 1065040250 cOmplete protease inhibitor cocktail Roche Cat # 4693159001 Phenylmethylsulfonyl Fluoride Sigma-Aldrich, Merck Cat # 52332-5GM Iodoacetamide Sigma-Aldrich, Merck Cat #I1149-5G Ethylenediaminetetraacetic acid Sigma-Aldrich, Merck Cat # EDS-100G (Continued on next page) e1 Cell Reports Medicine 5, 101516, May 21, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Sodium chloride Sigma-Aldrich, Merck Cat #S9888 Acetic acid Sigma-Aldrich, Merck Cat # 1000562500 PierceTM Trifluoroacetic Acid (TFA), LC-MS Grade Thermor Fisher Scientific Cat # 85183 Acetonitrile Sigma-Aldrich, Merck Cat # 1000292500 Triethylammonium bicarbonate buffer Sigma-Aldrich, Merck Cat #T7408- 100ML Urea Sigma-Aldrich, Merck Cat #U5378 endoLysC Wako Cat # 129-02541 Trypsin Promega Cat #V5111 Sodium dodecyl sulfate Sigma-Aldrich, Merck Cat #L4509 Tris(2-carboxyethyl)phosphine) Thermo Fisher Scientific Cat # 20491 Chloroacetamide Sigma-Aldrich, Merck Cat #C0267 Phosphoric acid Sigma-Aldrich, Merck Cat # 438081 Methanol (90%) Thermo Fisher Scientific Cat # A456-212 Formic Acid Thermo Fisher Scientific Cat # 006914143BS Acetonitrile Thermo Fisher Scientific Cat # A955-212 Trifluoroacetic acid Thermo Fisher Scientific Cat # 002023441A8BS Critical commercial assays Pan T cell Isolation Kit Miltenyi Biotec Cat # 130-096-535 CellTrace Violet Cell Proliferation kit Invitrogen Cat #C34557 ImmunoCult Human CD3/CD28/ CD2 T cell Activator Stemcell Technologies Cat # 10970 GolgiPlug Protein Transport Inhibitor BD Biosciences Cat # 555029 Fixability Viability Dye eFluor 506 Invitrogen Cat # 65-0866-14 Cytofix/Cytoperm Fixation/ Permeabilization kit BD Biosciences Cat # 554714 Human Single Cell Multiplexing kit BD Biosciences Cat # 633781 Tumor Dissociation kit Miltenyi Cat # 130-095-929 IFNg Secretion Assay Detection kit (PE), human Miltenyi Cat # 130-090-433 Optimal Cutting Temperature Compound Sakura Cat # 4583 Allprep DNA/RNA Micro kit Qiagen Cat # 80284 MagCore Genomic DNA Large Volume Whole Blood kit Atrida Cat # 104 SureSelect Human Alle Exon v7 low input kit Agilent Cat # 5191-4030 Illumina TruSeq RNA Exome kit Illumina Cat # 20020189, 20020490, 20020492, 20020183 SMARTer Stranded Total RNA Sample Prep kit – HI Mammalian Takara Cat # 634873 BD Human Sample Multiplexing Kit BD Biosciences Cat # 633781 BD RhapsodyTM WTA Amplification Kit BD Biosciences Cat # 633801 BD RhapsodyTM TCR/BCR Amplification Kit BD Biosciences Cat # 665345 TMT10plexTM Isobaric Label Reagents and Kits Thermo Fisher Scientific Cat # 90110 Deposited data UniProt SwissProt (version January 2022) UniProt consortium https://www.uniprot.org Human reference genome full hg38/GRCh38 1000 genomes http://ftp.1000genomes.ebi.ac.uk/ vol1/ftp/technical/reference/GRCh38_ reference_genome/ UniProt SwissProt (version Februari 2022) UniProt consortium https://www.uniprot.org Raw Single Cell RNA sequencing data This paper GEO: GSE240361 (Continued on next page) Cell Reports Medicine 5, 101516, May 21, 2024 e2 OPEN ACCESS

    Recombinant:

    Article Title: Neoantigen-targeted dendritic cell vaccination in lung cancer patients induces long-lived T cells exhibiting the full differentiation spectrum.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD3 PE-Cy7 Biolegend Cat # 300420; RRID: AB_439781 CD3 BV421 Biolegend Cat # 317343; RRID: AB_2565848 CD3 BV650 Biolegend Cat # 317323; RRID: AB_11126748 CD4 PerCP/Cy5.5 Biolegend Cat # 344608; RRID: AB_1953236 CD8 APC/Fire750 Biolegend Cat # 344746; RRID: AB_2572095 CD8 PerCP/Cy5.5 Biolegend Cat # 344709; RRID: AB_2044009 CD45 FITC This manuscript N/A CD95 PE/Dazzle594 Biolegend Cat # 305633; RRID: AB_2564220 CD127 BV711 Biolegend Cat # 351327; RRID: AB_11219191 CD137 PE Biolegend Cat # 309804; RRID: AB_314783 IFNg FITC Biolegend Cat # 502506; RRID: AB_315231 CX3CR1 BV421 BD Biosciences Cat # 565801; RRID: AB_2744471 CCR7 BV711 Biolegend Cat # 353228; RRID: AB_2563865 CCR7 APC/Fire750 Biolegend Cat # 353263; RRID: AB_2894483 Granzyme B PE-Cy7 Biolegend Cat # 372213; RRID: AB_2728380 W6/32 HB-95 (Hybridoma) N/A Biological samples Patient PBMC This manuscript N/A Patient tumor samples This manuscript N/A Patient PHA blasts This manuscript N/A PBMC derived from healthy blood donor Rode Kruis Vlaanderen N/A Chemicals, peptides, and recombinant proteins IL-7 Miltenyi Cat # 130-095-367 IL-15 Miltenyi Cat # 130-095-760 Neoantigen peptides for stimulation assays (crude) JPT Custom, PepTrack peptide library Neoantigen peptides for MHC class I tetramer generation (>80% pure) GenScript Custom Peptide-MHC class I tetramers NIH Tetramer Core facility N/A Dasatinib Stemcell Technologies Cat # 73082 Phytohemagglutinin Roche Cat # 11249738001 IL-2 Roche Cat # 11011456001 Nivolumab Bristol Myers Squibb N/A Tris MP Biomedicals Cat # 0210313305 Sodium tetraborate decahydrate Sigma-Aldrich, Merck Cat #B3545-500G Dimethyl pimelimidate dihydrochloride Sigma-Aldrich, Merck Cat #D8388-250MG Ethanolamine Thermo Fisher Scientific Cat # 149582500 Octyl b-D-glucopyranoside Sigma-Aldrich, Merck Cat #O9882-500MG Sodium deoxycholate Milipore, Merck Cat # 1065040250 cOmplete protease inhibitor cocktail Roche Cat # 4693159001 Phenylmethylsulfonyl Fluoride Sigma-Aldrich, Merck Cat # 52332-5GM Iodoacetamide Sigma-Aldrich, Merck Cat #I1149-5G Ethylenediaminetetraacetic acid Sigma-Aldrich, Merck Cat # EDS-100G (Continued on next page) e1 Cell Reports Medicine 5, 101516, May 21, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Sodium chloride Sigma-Aldrich, Merck Cat #S9888 Acetic acid Sigma-Aldrich, Merck Cat # 1000562500 PierceTM Trifluoroacetic Acid (TFA), LC-MS Grade Thermor Fisher Scientific Cat # 85183 Acetonitrile Sigma-Aldrich, Merck Cat # 1000292500 Triethylammonium bicarbonate buffer Sigma-Aldrich, Merck Cat #T7408- 100ML Urea Sigma-Aldrich, Merck Cat #U5378 endoLysC Wako Cat # 129-02541 Trypsin Promega Cat #V5111 Sodium dodecyl sulfate Sigma-Aldrich, Merck Cat #L4509 Tris(2-carboxyethyl)phosphine) Thermo Fisher Scientific Cat # 20491 Chloroacetamide Sigma-Aldrich, Merck Cat #C0267 Phosphoric acid Sigma-Aldrich, Merck Cat # 438081 Methanol (90%) Thermo Fisher Scientific Cat # A456-212 Formic Acid Thermo Fisher Scientific Cat # 006914143BS Acetonitrile Thermo Fisher Scientific Cat # A955-212 Trifluoroacetic acid Thermo Fisher Scientific Cat # 002023441A8BS Critical commercial assays Pan T cell Isolation Kit Miltenyi Biotec Cat # 130-096-535 CellTrace Violet Cell Proliferation kit Invitrogen Cat #C34557 ImmunoCult Human CD3/CD28/ CD2 T cell Activator Stemcell Technologies Cat # 10970 GolgiPlug Protein Transport Inhibitor BD Biosciences Cat # 555029 Fixability Viability Dye eFluor 506 Invitrogen Cat # 65-0866-14 Cytofix/Cytoperm Fixation/ Permeabilization kit BD Biosciences Cat # 554714 Human Single Cell Multiplexing kit BD Biosciences Cat # 633781 Tumor Dissociation kit Miltenyi Cat # 130-095-929 IFNg Secretion Assay Detection kit (PE), human Miltenyi Cat # 130-090-433 Optimal Cutting Temperature Compound Sakura Cat # 4583 Allprep DNA/RNA Micro kit Qiagen Cat # 80284 MagCore Genomic DNA Large Volume Whole Blood kit Atrida Cat # 104 SureSelect Human Alle Exon v7 low input kit Agilent Cat # 5191-4030 Illumina TruSeq RNA Exome kit Illumina Cat # 20020189, 20020490, 20020492, 20020183 SMARTer Stranded Total RNA Sample Prep kit – HI Mammalian Takara Cat # 634873 BD Human Sample Multiplexing Kit BD Biosciences Cat # 633781 BD RhapsodyTM WTA Amplification Kit BD Biosciences Cat # 633801 BD RhapsodyTM TCR/BCR Amplification Kit BD Biosciences Cat # 665345 TMT10plexTM Isobaric Label Reagents and Kits Thermo Fisher Scientific Cat # 90110 Deposited data UniProt SwissProt (version January 2022) UniProt consortium https://www.uniprot.org Human reference genome full hg38/GRCh38 1000 genomes http://ftp.1000genomes.ebi.ac.uk/ vol1/ftp/technical/reference/GRCh38_ reference_genome/ UniProt SwissProt (version Februari 2022) UniProt consortium https://www.uniprot.org Raw Single Cell RNA sequencing data This paper GEO: GSE240361 (Continued on next page) Cell Reports Medicine 5, 101516, May 21, 2024 e2 OPEN ACCESS

    Article Title: In Vivo Ultrasound and Photoacoustic Imaging of Nanoparticle-Engineered T Cells and Post-Treatment Assessment to Guide Adoptive Cell Immunotherapy.
    Article Snippet: .. Gold chloride trihydrate (HAuCl4, SigmaAldrich), sodium citrate tribasic dihydrate (Sigma-Aldrich), cetyltrimethylammonium bromide (CTAB, VWR), hydrochloric acid (HCl, Sigma-Aldrich), nitric acid (HNO3, GFS Chemicals), silver nitrate (AgNO3, Sigma-Aldrich), sodium borohydride (NaBH4, Sigma-Aldrich), hydroquinone (SigmaAldrich), tetraethyl orthosilicate (TEOS, Sigma-Aldrich), sodium hydroxide (NaOH, Sigma-Aldrich), methanol (Sigma-Aldrich), toluene (Sigma-Aldrich), silane-PEG-maleimide 2 kDa (MW 2000 Creative PEGWorks), polyethylene glycol thiol (mPEG-SH, MW:2000, Biochempeg), Dulbecco’s Modified Eagle Medium (DMEM, Cytvia), Roswell Park Memorial Institute 1640 medium (RPMI 1640, Cytvia), bovine serum albumin (BSA, Sigma-Aldrich), Cell Tracker Green CMFDA (Thermo Fisher Scientific), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, EMD Millipore), lactate dehydrogenase assay kit (LDH, Abcam), fetal bovine serum (FBS, Sigma-Aldrich), penicillin/streptomycin (Sigma-Aldrich), red blood cell (RBC) lysis buffer (BioLegend), nonessential amino acids (NEAA, Thermo Fisher Scientific), sodium pyruvate (Thermo Fisher Scientific), 2-mercaptoethanol (Sigma-Aldrich), recombinant murine SDF-1α (CXCL12, PeproTech), recombinant human interleukin-2 (IL-2; > 1 × 107 units/mg, PeproTech; or 1 × 106 units, Frederick National Laboratory), ovalbumin (OVA 257− 264, chicken, Sigma-Aldrich), phosphate buffered saline (PBS, Sigma-Aldrich), gelatin (MP Biomedicals), and silica (0.25 μm diameter, Sigma-Aldrich) were used in accordance with manufacturer’s instructions. ..

    Immunopeptidomics:

    Article Title: Neoantigen-targeted dendritic cell vaccination in lung cancer patients induces long-lived T cells exhibiting the full differentiation spectrum.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD3 PE-Cy7 Biolegend Cat # 300420; RRID: AB_439781 CD3 BV421 Biolegend Cat # 317343; RRID: AB_2565848 CD3 BV650 Biolegend Cat # 317323; RRID: AB_11126748 CD4 PerCP/Cy5.5 Biolegend Cat # 344608; RRID: AB_1953236 CD8 APC/Fire750 Biolegend Cat # 344746; RRID: AB_2572095 CD8 PerCP/Cy5.5 Biolegend Cat # 344709; RRID: AB_2044009 CD45 FITC This manuscript N/A CD95 PE/Dazzle594 Biolegend Cat # 305633; RRID: AB_2564220 CD127 BV711 Biolegend Cat # 351327; RRID: AB_11219191 CD137 PE Biolegend Cat # 309804; RRID: AB_314783 IFNg FITC Biolegend Cat # 502506; RRID: AB_315231 CX3CR1 BV421 BD Biosciences Cat # 565801; RRID: AB_2744471 CCR7 BV711 Biolegend Cat # 353228; RRID: AB_2563865 CCR7 APC/Fire750 Biolegend Cat # 353263; RRID: AB_2894483 Granzyme B PE-Cy7 Biolegend Cat # 372213; RRID: AB_2728380 W6/32 HB-95 (Hybridoma) N/A Biological samples Patient PBMC This manuscript N/A Patient tumor samples This manuscript N/A Patient PHA blasts This manuscript N/A PBMC derived from healthy blood donor Rode Kruis Vlaanderen N/A Chemicals, peptides, and recombinant proteins IL-7 Miltenyi Cat # 130-095-367 IL-15 Miltenyi Cat # 130-095-760 Neoantigen peptides for stimulation assays (crude) JPT Custom, PepTrack peptide library Neoantigen peptides for MHC class I tetramer generation (>80% pure) GenScript Custom Peptide-MHC class I tetramers NIH Tetramer Core facility N/A Dasatinib Stemcell Technologies Cat # 73082 Phytohemagglutinin Roche Cat # 11249738001 IL-2 Roche Cat # 11011456001 Nivolumab Bristol Myers Squibb N/A Tris MP Biomedicals Cat # 0210313305 Sodium tetraborate decahydrate Sigma-Aldrich, Merck Cat #B3545-500G Dimethyl pimelimidate dihydrochloride Sigma-Aldrich, Merck Cat #D8388-250MG Ethanolamine Thermo Fisher Scientific Cat # 149582500 Octyl b-D-glucopyranoside Sigma-Aldrich, Merck Cat #O9882-500MG Sodium deoxycholate Milipore, Merck Cat # 1065040250 cOmplete protease inhibitor cocktail Roche Cat # 4693159001 Phenylmethylsulfonyl Fluoride Sigma-Aldrich, Merck Cat # 52332-5GM Iodoacetamide Sigma-Aldrich, Merck Cat #I1149-5G Ethylenediaminetetraacetic acid Sigma-Aldrich, Merck Cat # EDS-100G (Continued on next page) e1 Cell Reports Medicine 5, 101516, May 21, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Sodium chloride Sigma-Aldrich, Merck Cat #S9888 Acetic acid Sigma-Aldrich, Merck Cat # 1000562500 PierceTM Trifluoroacetic Acid (TFA), LC-MS Grade Thermor Fisher Scientific Cat # 85183 Acetonitrile Sigma-Aldrich, Merck Cat # 1000292500 Triethylammonium bicarbonate buffer Sigma-Aldrich, Merck Cat #T7408- 100ML Urea Sigma-Aldrich, Merck Cat #U5378 endoLysC Wako Cat # 129-02541 Trypsin Promega Cat #V5111 Sodium dodecyl sulfate Sigma-Aldrich, Merck Cat #L4509 Tris(2-carboxyethyl)phosphine) Thermo Fisher Scientific Cat # 20491 Chloroacetamide Sigma-Aldrich, Merck Cat #C0267 Phosphoric acid Sigma-Aldrich, Merck Cat # 438081 Methanol (90%) Thermo Fisher Scientific Cat # A456-212 Formic Acid Thermo Fisher Scientific Cat # 006914143BS Acetonitrile Thermo Fisher Scientific Cat # A955-212 Trifluoroacetic acid Thermo Fisher Scientific Cat # 002023441A8BS Critical commercial assays Pan T cell Isolation Kit Miltenyi Biotec Cat # 130-096-535 CellTrace Violet Cell Proliferation kit Invitrogen Cat #C34557 ImmunoCult Human CD3/CD28/ CD2 T cell Activator Stemcell Technologies Cat # 10970 GolgiPlug Protein Transport Inhibitor BD Biosciences Cat # 555029 Fixability Viability Dye eFluor 506 Invitrogen Cat # 65-0866-14 Cytofix/Cytoperm Fixation/ Permeabilization kit BD Biosciences Cat # 554714 Human Single Cell Multiplexing kit BD Biosciences Cat # 633781 Tumor Dissociation kit Miltenyi Cat # 130-095-929 IFNg Secretion Assay Detection kit (PE), human Miltenyi Cat # 130-090-433 Optimal Cutting Temperature Compound Sakura Cat # 4583 Allprep DNA/RNA Micro kit Qiagen Cat # 80284 MagCore Genomic DNA Large Volume Whole Blood kit Atrida Cat # 104 SureSelect Human Alle Exon v7 low input kit Agilent Cat # 5191-4030 Illumina TruSeq RNA Exome kit Illumina Cat # 20020189, 20020490, 20020492, 20020183 SMARTer Stranded Total RNA Sample Prep kit – HI Mammalian Takara Cat # 634873 BD Human Sample Multiplexing Kit BD Biosciences Cat # 633781 BD RhapsodyTM WTA Amplification Kit BD Biosciences Cat # 633801 BD RhapsodyTM TCR/BCR Amplification Kit BD Biosciences Cat # 665345 TMT10plexTM Isobaric Label Reagents and Kits Thermo Fisher Scientific Cat # 90110 Deposited data UniProt SwissProt (version January 2022) UniProt consortium https://www.uniprot.org Human reference genome full hg38/GRCh38 1000 genomes http://ftp.1000genomes.ebi.ac.uk/ vol1/ftp/technical/reference/GRCh38_ reference_genome/ UniProt SwissProt (version Februari 2022) UniProt consortium https://www.uniprot.org Raw Single Cell RNA sequencing data This paper GEO: GSE240361 (Continued on next page) Cell Reports Medicine 5, 101516, May 21, 2024 e2 OPEN ACCESS

    Protease Inhibitor:

    Article Title: Neoantigen-targeted dendritic cell vaccination in lung cancer patients induces long-lived T cells exhibiting the full differentiation spectrum.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD3 PE-Cy7 Biolegend Cat # 300420; RRID: AB_439781 CD3 BV421 Biolegend Cat # 317343; RRID: AB_2565848 CD3 BV650 Biolegend Cat # 317323; RRID: AB_11126748 CD4 PerCP/Cy5.5 Biolegend Cat # 344608; RRID: AB_1953236 CD8 APC/Fire750 Biolegend Cat # 344746; RRID: AB_2572095 CD8 PerCP/Cy5.5 Biolegend Cat # 344709; RRID: AB_2044009 CD45 FITC This manuscript N/A CD95 PE/Dazzle594 Biolegend Cat # 305633; RRID: AB_2564220 CD127 BV711 Biolegend Cat # 351327; RRID: AB_11219191 CD137 PE Biolegend Cat # 309804; RRID: AB_314783 IFNg FITC Biolegend Cat # 502506; RRID: AB_315231 CX3CR1 BV421 BD Biosciences Cat # 565801; RRID: AB_2744471 CCR7 BV711 Biolegend Cat # 353228; RRID: AB_2563865 CCR7 APC/Fire750 Biolegend Cat # 353263; RRID: AB_2894483 Granzyme B PE-Cy7 Biolegend Cat # 372213; RRID: AB_2728380 W6/32 HB-95 (Hybridoma) N/A Biological samples Patient PBMC This manuscript N/A Patient tumor samples This manuscript N/A Patient PHA blasts This manuscript N/A PBMC derived from healthy blood donor Rode Kruis Vlaanderen N/A Chemicals, peptides, and recombinant proteins IL-7 Miltenyi Cat # 130-095-367 IL-15 Miltenyi Cat # 130-095-760 Neoantigen peptides for stimulation assays (crude) JPT Custom, PepTrack peptide library Neoantigen peptides for MHC class I tetramer generation (>80% pure) GenScript Custom Peptide-MHC class I tetramers NIH Tetramer Core facility N/A Dasatinib Stemcell Technologies Cat # 73082 Phytohemagglutinin Roche Cat # 11249738001 IL-2 Roche Cat # 11011456001 Nivolumab Bristol Myers Squibb N/A Tris MP Biomedicals Cat # 0210313305 Sodium tetraborate decahydrate Sigma-Aldrich, Merck Cat #B3545-500G Dimethyl pimelimidate dihydrochloride Sigma-Aldrich, Merck Cat #D8388-250MG Ethanolamine Thermo Fisher Scientific Cat # 149582500 Octyl b-D-glucopyranoside Sigma-Aldrich, Merck Cat #O9882-500MG Sodium deoxycholate Milipore, Merck Cat # 1065040250 cOmplete protease inhibitor cocktail Roche Cat # 4693159001 Phenylmethylsulfonyl Fluoride Sigma-Aldrich, Merck Cat # 52332-5GM Iodoacetamide Sigma-Aldrich, Merck Cat #I1149-5G Ethylenediaminetetraacetic acid Sigma-Aldrich, Merck Cat # EDS-100G (Continued on next page) e1 Cell Reports Medicine 5, 101516, May 21, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Sodium chloride Sigma-Aldrich, Merck Cat #S9888 Acetic acid Sigma-Aldrich, Merck Cat # 1000562500 PierceTM Trifluoroacetic Acid (TFA), LC-MS Grade Thermor Fisher Scientific Cat # 85183 Acetonitrile Sigma-Aldrich, Merck Cat # 1000292500 Triethylammonium bicarbonate buffer Sigma-Aldrich, Merck Cat #T7408- 100ML Urea Sigma-Aldrich, Merck Cat #U5378 endoLysC Wako Cat # 129-02541 Trypsin Promega Cat #V5111 Sodium dodecyl sulfate Sigma-Aldrich, Merck Cat #L4509 Tris(2-carboxyethyl)phosphine) Thermo Fisher Scientific Cat # 20491 Chloroacetamide Sigma-Aldrich, Merck Cat #C0267 Phosphoric acid Sigma-Aldrich, Merck Cat # 438081 Methanol (90%) Thermo Fisher Scientific Cat # A456-212 Formic Acid Thermo Fisher Scientific Cat # 006914143BS Acetonitrile Thermo Fisher Scientific Cat # A955-212 Trifluoroacetic acid Thermo Fisher Scientific Cat # 002023441A8BS Critical commercial assays Pan T cell Isolation Kit Miltenyi Biotec Cat # 130-096-535 CellTrace Violet Cell Proliferation kit Invitrogen Cat #C34557 ImmunoCult Human CD3/CD28/ CD2 T cell Activator Stemcell Technologies Cat # 10970 GolgiPlug Protein Transport Inhibitor BD Biosciences Cat # 555029 Fixability Viability Dye eFluor 506 Invitrogen Cat # 65-0866-14 Cytofix/Cytoperm Fixation/ Permeabilization kit BD Biosciences Cat # 554714 Human Single Cell Multiplexing kit BD Biosciences Cat # 633781 Tumor Dissociation kit Miltenyi Cat # 130-095-929 IFNg Secretion Assay Detection kit (PE), human Miltenyi Cat # 130-090-433 Optimal Cutting Temperature Compound Sakura Cat # 4583 Allprep DNA/RNA Micro kit Qiagen Cat # 80284 MagCore Genomic DNA Large Volume Whole Blood kit Atrida Cat # 104 SureSelect Human Alle Exon v7 low input kit Agilent Cat # 5191-4030 Illumina TruSeq RNA Exome kit Illumina Cat # 20020189, 20020490, 20020492, 20020183 SMARTer Stranded Total RNA Sample Prep kit – HI Mammalian Takara Cat # 634873 BD Human Sample Multiplexing Kit BD Biosciences Cat # 633781 BD RhapsodyTM WTA Amplification Kit BD Biosciences Cat # 633801 BD RhapsodyTM TCR/BCR Amplification Kit BD Biosciences Cat # 665345 TMT10plexTM Isobaric Label Reagents and Kits Thermo Fisher Scientific Cat # 90110 Deposited data UniProt SwissProt (version January 2022) UniProt consortium https://www.uniprot.org Human reference genome full hg38/GRCh38 1000 genomes http://ftp.1000genomes.ebi.ac.uk/ vol1/ftp/technical/reference/GRCh38_ reference_genome/ UniProt SwissProt (version Februari 2022) UniProt consortium https://www.uniprot.org Raw Single Cell RNA sequencing data This paper GEO: GSE240361 (Continued on next page) Cell Reports Medicine 5, 101516, May 21, 2024 e2 OPEN ACCESS

    Modification:

    Article Title: In Vivo Ultrasound and Photoacoustic Imaging of Nanoparticle-Engineered T Cells and Post-Treatment Assessment to Guide Adoptive Cell Immunotherapy.
    Article Snippet: .. Gold chloride trihydrate (HAuCl4, SigmaAldrich), sodium citrate tribasic dihydrate (Sigma-Aldrich), cetyltrimethylammonium bromide (CTAB, VWR), hydrochloric acid (HCl, Sigma-Aldrich), nitric acid (HNO3, GFS Chemicals), silver nitrate (AgNO3, Sigma-Aldrich), sodium borohydride (NaBH4, Sigma-Aldrich), hydroquinone (SigmaAldrich), tetraethyl orthosilicate (TEOS, Sigma-Aldrich), sodium hydroxide (NaOH, Sigma-Aldrich), methanol (Sigma-Aldrich), toluene (Sigma-Aldrich), silane-PEG-maleimide 2 kDa (MW 2000 Creative PEGWorks), polyethylene glycol thiol (mPEG-SH, MW:2000, Biochempeg), Dulbecco’s Modified Eagle Medium (DMEM, Cytvia), Roswell Park Memorial Institute 1640 medium (RPMI 1640, Cytvia), bovine serum albumin (BSA, Sigma-Aldrich), Cell Tracker Green CMFDA (Thermo Fisher Scientific), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, EMD Millipore), lactate dehydrogenase assay kit (LDH, Abcam), fetal bovine serum (FBS, Sigma-Aldrich), penicillin/streptomycin (Sigma-Aldrich), red blood cell (RBC) lysis buffer (BioLegend), nonessential amino acids (NEAA, Thermo Fisher Scientific), sodium pyruvate (Thermo Fisher Scientific), 2-mercaptoethanol (Sigma-Aldrich), recombinant murine SDF-1α (CXCL12, PeproTech), recombinant human interleukin-2 (IL-2; > 1 × 107 units/mg, PeproTech; or 1 × 106 units, Frederick National Laboratory), ovalbumin (OVA 257− 264, chicken, Sigma-Aldrich), phosphate buffered saline (PBS, Sigma-Aldrich), gelatin (MP Biomedicals), and silica (0.25 μm diameter, Sigma-Aldrich) were used in accordance with manufacturer’s instructions. ..

    MTT Assay:

    Article Title: In Vivo Ultrasound and Photoacoustic Imaging of Nanoparticle-Engineered T Cells and Post-Treatment Assessment to Guide Adoptive Cell Immunotherapy.
    Article Snippet: .. Gold chloride trihydrate (HAuCl4, SigmaAldrich), sodium citrate tribasic dihydrate (Sigma-Aldrich), cetyltrimethylammonium bromide (CTAB, VWR), hydrochloric acid (HCl, Sigma-Aldrich), nitric acid (HNO3, GFS Chemicals), silver nitrate (AgNO3, Sigma-Aldrich), sodium borohydride (NaBH4, Sigma-Aldrich), hydroquinone (SigmaAldrich), tetraethyl orthosilicate (TEOS, Sigma-Aldrich), sodium hydroxide (NaOH, Sigma-Aldrich), methanol (Sigma-Aldrich), toluene (Sigma-Aldrich), silane-PEG-maleimide 2 kDa (MW 2000 Creative PEGWorks), polyethylene glycol thiol (mPEG-SH, MW:2000, Biochempeg), Dulbecco’s Modified Eagle Medium (DMEM, Cytvia), Roswell Park Memorial Institute 1640 medium (RPMI 1640, Cytvia), bovine serum albumin (BSA, Sigma-Aldrich), Cell Tracker Green CMFDA (Thermo Fisher Scientific), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, EMD Millipore), lactate dehydrogenase assay kit (LDH, Abcam), fetal bovine serum (FBS, Sigma-Aldrich), penicillin/streptomycin (Sigma-Aldrich), red blood cell (RBC) lysis buffer (BioLegend), nonessential amino acids (NEAA, Thermo Fisher Scientific), sodium pyruvate (Thermo Fisher Scientific), 2-mercaptoethanol (Sigma-Aldrich), recombinant murine SDF-1α (CXCL12, PeproTech), recombinant human interleukin-2 (IL-2; > 1 × 107 units/mg, PeproTech; or 1 × 106 units, Frederick National Laboratory), ovalbumin (OVA 257− 264, chicken, Sigma-Aldrich), phosphate buffered saline (PBS, Sigma-Aldrich), gelatin (MP Biomedicals), and silica (0.25 μm diameter, Sigma-Aldrich) were used in accordance with manufacturer’s instructions. ..

    Lactate Dehydrogenase Assay:

    Article Title: In Vivo Ultrasound and Photoacoustic Imaging of Nanoparticle-Engineered T Cells and Post-Treatment Assessment to Guide Adoptive Cell Immunotherapy.
    Article Snippet: .. Gold chloride trihydrate (HAuCl4, SigmaAldrich), sodium citrate tribasic dihydrate (Sigma-Aldrich), cetyltrimethylammonium bromide (CTAB, VWR), hydrochloric acid (HCl, Sigma-Aldrich), nitric acid (HNO3, GFS Chemicals), silver nitrate (AgNO3, Sigma-Aldrich), sodium borohydride (NaBH4, Sigma-Aldrich), hydroquinone (SigmaAldrich), tetraethyl orthosilicate (TEOS, Sigma-Aldrich), sodium hydroxide (NaOH, Sigma-Aldrich), methanol (Sigma-Aldrich), toluene (Sigma-Aldrich), silane-PEG-maleimide 2 kDa (MW 2000 Creative PEGWorks), polyethylene glycol thiol (mPEG-SH, MW:2000, Biochempeg), Dulbecco’s Modified Eagle Medium (DMEM, Cytvia), Roswell Park Memorial Institute 1640 medium (RPMI 1640, Cytvia), bovine serum albumin (BSA, Sigma-Aldrich), Cell Tracker Green CMFDA (Thermo Fisher Scientific), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, EMD Millipore), lactate dehydrogenase assay kit (LDH, Abcam), fetal bovine serum (FBS, Sigma-Aldrich), penicillin/streptomycin (Sigma-Aldrich), red blood cell (RBC) lysis buffer (BioLegend), nonessential amino acids (NEAA, Thermo Fisher Scientific), sodium pyruvate (Thermo Fisher Scientific), 2-mercaptoethanol (Sigma-Aldrich), recombinant murine SDF-1α (CXCL12, PeproTech), recombinant human interleukin-2 (IL-2; > 1 × 107 units/mg, PeproTech; or 1 × 106 units, Frederick National Laboratory), ovalbumin (OVA 257− 264, chicken, Sigma-Aldrich), phosphate buffered saline (PBS, Sigma-Aldrich), gelatin (MP Biomedicals), and silica (0.25 μm diameter, Sigma-Aldrich) were used in accordance with manufacturer’s instructions. ..

    Red Blood Cell Lysis:

    Article Title: In Vivo Ultrasound and Photoacoustic Imaging of Nanoparticle-Engineered T Cells and Post-Treatment Assessment to Guide Adoptive Cell Immunotherapy.
    Article Snippet: .. Gold chloride trihydrate (HAuCl4, SigmaAldrich), sodium citrate tribasic dihydrate (Sigma-Aldrich), cetyltrimethylammonium bromide (CTAB, VWR), hydrochloric acid (HCl, Sigma-Aldrich), nitric acid (HNO3, GFS Chemicals), silver nitrate (AgNO3, Sigma-Aldrich), sodium borohydride (NaBH4, Sigma-Aldrich), hydroquinone (SigmaAldrich), tetraethyl orthosilicate (TEOS, Sigma-Aldrich), sodium hydroxide (NaOH, Sigma-Aldrich), methanol (Sigma-Aldrich), toluene (Sigma-Aldrich), silane-PEG-maleimide 2 kDa (MW 2000 Creative PEGWorks), polyethylene glycol thiol (mPEG-SH, MW:2000, Biochempeg), Dulbecco’s Modified Eagle Medium (DMEM, Cytvia), Roswell Park Memorial Institute 1640 medium (RPMI 1640, Cytvia), bovine serum albumin (BSA, Sigma-Aldrich), Cell Tracker Green CMFDA (Thermo Fisher Scientific), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, EMD Millipore), lactate dehydrogenase assay kit (LDH, Abcam), fetal bovine serum (FBS, Sigma-Aldrich), penicillin/streptomycin (Sigma-Aldrich), red blood cell (RBC) lysis buffer (BioLegend), nonessential amino acids (NEAA, Thermo Fisher Scientific), sodium pyruvate (Thermo Fisher Scientific), 2-mercaptoethanol (Sigma-Aldrich), recombinant murine SDF-1α (CXCL12, PeproTech), recombinant human interleukin-2 (IL-2; > 1 × 107 units/mg, PeproTech; or 1 × 106 units, Frederick National Laboratory), ovalbumin (OVA 257− 264, chicken, Sigma-Aldrich), phosphate buffered saline (PBS, Sigma-Aldrich), gelatin (MP Biomedicals), and silica (0.25 μm diameter, Sigma-Aldrich) were used in accordance with manufacturer’s instructions. ..

    Saline:

    Article Title: In Vivo Ultrasound and Photoacoustic Imaging of Nanoparticle-Engineered T Cells and Post-Treatment Assessment to Guide Adoptive Cell Immunotherapy.
    Article Snippet: .. Gold chloride trihydrate (HAuCl4, SigmaAldrich), sodium citrate tribasic dihydrate (Sigma-Aldrich), cetyltrimethylammonium bromide (CTAB, VWR), hydrochloric acid (HCl, Sigma-Aldrich), nitric acid (HNO3, GFS Chemicals), silver nitrate (AgNO3, Sigma-Aldrich), sodium borohydride (NaBH4, Sigma-Aldrich), hydroquinone (SigmaAldrich), tetraethyl orthosilicate (TEOS, Sigma-Aldrich), sodium hydroxide (NaOH, Sigma-Aldrich), methanol (Sigma-Aldrich), toluene (Sigma-Aldrich), silane-PEG-maleimide 2 kDa (MW 2000 Creative PEGWorks), polyethylene glycol thiol (mPEG-SH, MW:2000, Biochempeg), Dulbecco’s Modified Eagle Medium (DMEM, Cytvia), Roswell Park Memorial Institute 1640 medium (RPMI 1640, Cytvia), bovine serum albumin (BSA, Sigma-Aldrich), Cell Tracker Green CMFDA (Thermo Fisher Scientific), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, EMD Millipore), lactate dehydrogenase assay kit (LDH, Abcam), fetal bovine serum (FBS, Sigma-Aldrich), penicillin/streptomycin (Sigma-Aldrich), red blood cell (RBC) lysis buffer (BioLegend), nonessential amino acids (NEAA, Thermo Fisher Scientific), sodium pyruvate (Thermo Fisher Scientific), 2-mercaptoethanol (Sigma-Aldrich), recombinant murine SDF-1α (CXCL12, PeproTech), recombinant human interleukin-2 (IL-2; > 1 × 107 units/mg, PeproTech; or 1 × 106 units, Frederick National Laboratory), ovalbumin (OVA 257− 264, chicken, Sigma-Aldrich), phosphate buffered saline (PBS, Sigma-Aldrich), gelatin (MP Biomedicals), and silica (0.25 μm diameter, Sigma-Aldrich) were used in accordance with manufacturer’s instructions. ..

    Cell Culture:

    Article Title: Glycolysis promotes caspase-3 activation in lipid rafts in T cells.
    Article Snippet: .. Cells were then washed three times and cultured in RPMI-C medium with 50 U/mL IL-2 and the indicated doses of 2-deoxy-D-glucose (SigmaAldrich, St. Louis, MO, USA) or rapamycin (MP Biomedicals, Solon, OH, USA) for 3 days, replacing media, cytokine, and inhibitor each day. .. IL-2-cultured T cells cultured in 2-deoxy-D-glucose were centrifuged over Histopaque-1077 (Sigma-Aldrich) to minimize the number of dead cells in the cultures prior to use in the assays.



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    Levels of inflammatory cytokines was measured by ELISA in DSS-induced ulcerative colitis. ( A ) The level of inflammatory cytokines such as IL-2, IL-4, IL-6, IL-10, IL-22, or 5-HT from the serum of the mice. ( B ) The level of inflammatory cytokines such as IL-2, IL-4, IL-6, IL-10, IL-22, TNF-α, IFN-γ, or 5-HT from the colon of the mice. The graphs are represented as mean ±SD. * p <0.05, ** p <0.01 and *** p <0.001.

    Journal: Journal of Inflammation Research

    Article Title: Dynamic Changes in Gut Microbiome of Ulcerative Colitis: Initial Study from Animal Model

    doi: 10.2147/JIR.S358807

    Figure Lengend Snippet: Levels of inflammatory cytokines was measured by ELISA in DSS-induced ulcerative colitis. ( A ) The level of inflammatory cytokines such as IL-2, IL-4, IL-6, IL-10, IL-22, or 5-HT from the serum of the mice. ( B ) The level of inflammatory cytokines such as IL-2, IL-4, IL-6, IL-10, IL-22, TNF-α, IFN-γ, or 5-HT from the colon of the mice. The graphs are represented as mean ±SD. * p <0.05, ** p <0.01 and *** p <0.001.

    Article Snippet: The following reagents and instruments were used: DSS (molecular weight = 36 kDa to 50 kDa; MP Biomedical, California, USA) and enzyme-linked immunosorbent assay (ELISA) kits: interleukin-2 (IL-2), interleukin-4 (IL-4), interleukin-6 (IL-6), interleukin-10 (IL-10), interleukin-22 (IL-22), interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), 5-hydroxytryptamine (5-HT) (Neobioscience Technology Company, Shanghai, China).

    Techniques: Enzyme-linked Immunosorbent Assay