Review




Structured Review

Valiant Co Ltd carrier ampholyte
Carrier Ampholyte, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0215355583/Nortriptyline+hydrochloride/pm36943849-99-27-52
Average 93 stars, based on 23 article reviews
carrier ampholyte - by Bioz Stars, 2026-09
93/100 stars

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Related Articles

Incubation:

Article Title: Phosphorylation of Cx43 residue Y313 by Src contributes to blocking the interaction with Drebrin and disassembling gap junctions
Article Snippet: Slides were washed again with TBS-T and blocked (1× TBS with 2.5% horse serum and 0.2% Triton) for 1 h at RT and incubated at 4 °C overnight with the following primary antibodies: α-Cx43 (1:50, ab87645 Abcam) and α-Cx43 pY313 (1:100). .. The next day, sections were washed 3 × 10 min with 1× TBS-T, incubated with secondary antibody (ab150130 and ab150079, Abcam) for 1 h at RT, stained with DAPI (100 ng/mL) (MP Biomedicals) for 10 min, and then washed 3 × 10 min with 1× TBS-T. Coverslips were mounted on a drop of SlowFade anti-fade (Life Tech), sealed with clear nail polish, and imaged. ..

Article Title: Phosphorylation of Cx43 residue Y313 by Src contributes to blocking the interaction with Drebrin and disassembling gap junctions
Article Snippet: .. Coverslips were washed 3 × 10 min with 1× TBS, incubated with secondary antibody for 1 h at RT, stained with DAPI (100 ng/mL) (MP Biomedicals) for 10 min, and then washed 3 × 10 min with 1× TBS. .. Coverslips were mounted on a drop of SlowFade anti-fade (Life Tech), sealed with clear nail polish, and imaged.

Staining:

Article Title: Phosphorylation of Cx43 residue Y313 by Src contributes to blocking the interaction with Drebrin and disassembling gap junctions
Article Snippet: Slides were washed again with TBS-T and blocked (1× TBS with 2.5% horse serum and 0.2% Triton) for 1 h at RT and incubated at 4 °C overnight with the following primary antibodies: α-Cx43 (1:50, ab87645 Abcam) and α-Cx43 pY313 (1:100). .. The next day, sections were washed 3 × 10 min with 1× TBS-T, incubated with secondary antibody (ab150130 and ab150079, Abcam) for 1 h at RT, stained with DAPI (100 ng/mL) (MP Biomedicals) for 10 min, and then washed 3 × 10 min with 1× TBS-T. Coverslips were mounted on a drop of SlowFade anti-fade (Life Tech), sealed with clear nail polish, and imaged. ..

Article Title: Phosphorylation of Cx43 residue Y313 by Src contributes to blocking the interaction with Drebrin and disassembling gap junctions
Article Snippet: .. Coverslips were washed 3 × 10 min with 1× TBS, incubated with secondary antibody for 1 h at RT, stained with DAPI (100 ng/mL) (MP Biomedicals) for 10 min, and then washed 3 × 10 min with 1× TBS. .. Coverslips were mounted on a drop of SlowFade anti-fade (Life Tech), sealed with clear nail polish, and imaged.

Sonication:

Article Title: New insights into the role of microheterogeneity of ZP3 during structural maturation of the avian equivalent of mammalian zona pellucida.
Article Snippet: Preparative liquid-phase isoelectric focusing of chicken ZP3 isoforms Fractionation of ZP3 isoforms was performed using MicroRotoforTM Liquid-Phase IEF Cell (Bio-Rad, Hercules, CA) according to the manufacturer’s instructions. .. The chicken IPVL (33 or 29 μg of wet weight) from which the ZPD was dissociated and removed by sonication and centrifugation [18] was solubilized in 8% carrier ampholyte (Bio-Lyte1 3/10 or 5/7 Ampholytes for pH range of ~3.5–9.5 or ~5–7, respectively; Bio-Rad) containing 3% Triton X-100 and 8M urea (ultra-pure grade; MP Biomedicals, Irvine, CA) and subjected to the liquid-phase isoelectric focusing (LP-IEF). ..

Article Title: New insights into the role of microheterogeneity of ZP3 during structural maturation of the avian equivalent of mammalian zona pellucida
Article Snippet: Fractionation of ZP3 isoforms was performed using MicroRotofor TM Liquid-Phase IEF Cell (Bio-Rad, Hercules, CA) according to the manufacturer’s instructions. .. The chicken IPVL (33 or 29 μg of wet weight) from which the ZPD was dissociated and removed by sonication and centrifugation [ ] was solubilized in 8% carrier ampholyte (Bio-Lyte ® 3/10 or 5/7 Ampholytes for pH range of ~3.5–9.5 or ~5–7, respectively; Bio-Rad) containing 3% Triton X-100 and 8M urea (ultra-pure grade; MP Biomedicals, Irvine, CA) and subjected to the liquid-phase isoelectric focusing (LP-IEF). ..

Centrifugation:

Article Title: New insights into the role of microheterogeneity of ZP3 during structural maturation of the avian equivalent of mammalian zona pellucida.
Article Snippet: Preparative liquid-phase isoelectric focusing of chicken ZP3 isoforms Fractionation of ZP3 isoforms was performed using MicroRotoforTM Liquid-Phase IEF Cell (Bio-Rad, Hercules, CA) according to the manufacturer’s instructions. .. The chicken IPVL (33 or 29 μg of wet weight) from which the ZPD was dissociated and removed by sonication and centrifugation [18] was solubilized in 8% carrier ampholyte (Bio-Lyte1 3/10 or 5/7 Ampholytes for pH range of ~3.5–9.5 or ~5–7, respectively; Bio-Rad) containing 3% Triton X-100 and 8M urea (ultra-pure grade; MP Biomedicals, Irvine, CA) and subjected to the liquid-phase isoelectric focusing (LP-IEF). ..

Article Title: New insights into the role of microheterogeneity of ZP3 during structural maturation of the avian equivalent of mammalian zona pellucida
Article Snippet: Fractionation of ZP3 isoforms was performed using MicroRotofor TM Liquid-Phase IEF Cell (Bio-Rad, Hercules, CA) according to the manufacturer’s instructions. .. The chicken IPVL (33 or 29 μg of wet weight) from which the ZPD was dissociated and removed by sonication and centrifugation [ ] was solubilized in 8% carrier ampholyte (Bio-Lyte ® 3/10 or 5/7 Ampholytes for pH range of ~3.5–9.5 or ~5–7, respectively; Bio-Rad) containing 3% Triton X-100 and 8M urea (ultra-pure grade; MP Biomedicals, Irvine, CA) and subjected to the liquid-phase isoelectric focusing (LP-IEF). ..



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