Review



e 64  (Valiant Co Ltd)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 91
      Buy from Supplier

    Structured Review

    Valiant Co Ltd e 64
    E 64, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 91/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/0215284601/E-64/pm26086376-223-37-38
    Average 91 stars, based on 16 article reviews
    e 64 - by Bioz Stars, 2026-09
    91/100 stars

    Images

    Related Articles

    Protease Inhibitor:

    Article Title: Inhibition of Hyaluronan Synthesis Reduces Versican and Fibronectin Levels in Trabecular Meshwork Cells
    Article Snippet: .. For hyaluronidase treatments, hyaluronidase (1 unit in PBS; from Streptomyces hyalurolyticus; MP Biomedicals, Solon, OH) was added to confluent TM cells in serum-free media each day for 3 days in the presence of protease inhibitor cocktail for tissue culture (contains aprotinin, bestatin, E-64, leupeptin and pepstatin A; Sigma). .. Total RNA was isolated from 4MU-treated or vehicle control-treated TM cells using cells-to-cDNA lysis buffer (Ambion, Austin, TX) and cDNA was generated using Superscript III reverse transcriptase (Invitrogen, Carlsbad, CA).

    Lysis:

    Article Title: Cryo-Electron Microscopy Snapshots of Eukaryotic Membrane Proteins in Native Lipid-Bilayer Nanodiscs.
    Article Snippet: New technologies for purifying membrane-bound protein complexes in combination with cryo-electron microscopy (EM) have recently allowed the exploration of such complexes under near-native conditions.. In particular, polymer-encapsulated nanodiscs enable the study of membrane proteins at high resolution while retaining protein−protein and protein−lipid interactions within a lipid bilayer.. However, this powerful technology has not been exploited to address the important question of how endogenous�as opposed to overexpressed�membrane proteins are organized within a lipid environment.

    other:

    Article Title: Stage-specific expression of protease genes in the apicomplexan parasite, Eimeria tenella
    Article Snippet: E-64 , Cysteine , 10 μM , MP Biomedicals.



    Similar Products

    lysis buffer  (Valiant Co Ltd)
    91
    Valiant Co Ltd lysis buffer
    Lysis Buffer, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/0215284601/E-64/pm36399657-56-8-67
    Average 91 stars, based on 1 article reviews
    lysis buffer - by Bioz Stars, 2026-09
    91/100 stars
    		  Buy from Supplier
    protease inhibitor cocktail  (Valiant Co Ltd)
    91
    Valiant Co Ltd protease inhibitor cocktail
    Protease Inhibitor Cocktail, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/0215284601/E-64/pmc03489675-155-33-11
    Average 91 stars, based on 1 article reviews
    protease inhibitor cocktail - by Bioz Stars, 2026-09
    91/100 stars
    		  Buy from Supplier
    e 64  (Valiant Co Ltd)
    91
    Valiant Co Ltd e 64
    E 64, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/0215284601/E-64/pmc03770453-100-0-7
    Average 91 stars, based on 1 article reviews
    e 64 - by Bioz Stars, 2026-09
    91/100 stars
    		  Buy from Supplier
    cysteine  (Valiant Co Ltd)
    91
    Valiant Co Ltd cysteine
    Cysteine, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/0215284601/E-64/pmc03770453-100-2-7
    Average 91 stars, based on 1 article reviews
    cysteine - by Bioz Stars, 2026-09
    91/100 stars
    		  Buy from Supplier
    e 64 controls  (Valiant Co Ltd)
    91
    Valiant Co Ltd e 64 controls
    hnf4 is required for blood feeding a , Representative brightfield micrographs of live control(RNAi) animals or hnf4(RNAi) animals that were cultured in media containing bovine red blood cells. The inset in the right panel shows a magnification of the indicated area of the gut filled with undigested hemoglobin as evidenced by the red pigmentation. The number of parasites similar to the representative micrograph is indicated in the upper right of each panel. b , Pie chart depicting the frequency of different gut pigmentation of animals from a . n = 69 control(RNAi) animals and 69 hnf4(RNAi) animals from three biological replicates. c , Graph of the cysteine protease cathepsin activity of lysates from control(RNAi) animals or hnf4(RNAi) animals as determined by the ability to cleave the fluorogenic substrate, Z-FR-AMC, in the presence of no inhibitor (DMSO), the general cysteine protease inhibitor, <t>E-64,</t> or the cathepsin B-selective inhibitor, CA-074. Data are from three biological replicates each in triplicate. d , Representative micrographs of H&E-stained sections of mouse livers 22 days after transplant with RNAi-treated parasites. No granulomata are present in the livers of mice that received hnf4(RNAi) parasites. The number of sections similar to the representative micrograph is indicated in the upper right each panel. Data are from three recipients from one biological replicate. e , Representative image of DAPI-stained parasites recovered from mice 22 days after transplant with RNAi-treated parasites. The number of parasites grossly similar to the representative micrograph is indicated in the upper right of each panel. Data are from parasites perfused from three separate recipients. Nuclei are pseudo-colored grey. Scale bars, a , 100µm, e , 100µm, f , 1mm. ****, p <0.0001 (Welch’s t-test).
    E 64 Controls, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/0215284601/E-64/bio_rxiv__2020__02__03__932004-187-6-2
    Average 91 stars, based on 1 article reviews
    e 64 controls - by Bioz Stars, 2026-09
    91/100 stars
    		  Buy from Supplier
    e64 c  (Valiant Co Ltd)
    91
    Valiant Co Ltd e64 c
    hnf4 is required for blood feeding a , Representative brightfield micrographs of live control(RNAi) animals or hnf4(RNAi) animals that were cultured in media containing bovine red blood cells. The inset in the right panel shows a magnification of the indicated area of the gut filled with undigested hemoglobin as evidenced by the red pigmentation. The number of parasites similar to the representative micrograph is indicated in the upper right of each panel. b , Pie chart depicting the frequency of different gut pigmentation of animals from a . n = 69 control(RNAi) animals and 69 hnf4(RNAi) animals from three biological replicates. c , Graph of the cysteine protease cathepsin activity of lysates from control(RNAi) animals or hnf4(RNAi) animals as determined by the ability to cleave the fluorogenic substrate, Z-FR-AMC, in the presence of no inhibitor (DMSO), the general cysteine protease inhibitor, <t>E-64,</t> or the cathepsin B-selective inhibitor, CA-074. Data are from three biological replicates each in triplicate. d , Representative micrographs of H&E-stained sections of mouse livers 22 days after transplant with RNAi-treated parasites. No granulomata are present in the livers of mice that received hnf4(RNAi) parasites. The number of sections similar to the representative micrograph is indicated in the upper right each panel. Data are from three recipients from one biological replicate. e , Representative image of DAPI-stained parasites recovered from mice 22 days after transplant with RNAi-treated parasites. The number of parasites grossly similar to the representative micrograph is indicated in the upper right of each panel. Data are from parasites perfused from three separate recipients. Nuclei are pseudo-colored grey. Scale bars, a , 100µm, e , 100µm, f , 1mm. ****, p <0.0001 (Welch’s t-test).
    E64 C, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/0215284601/E-64/jiang_zhenze__2020__biochemical_characterization_of_proteolytic_activities_and_substrate_specificities-1142-41-51
    Average 91 stars, based on 1 article reviews
    e64 c - by Bioz Stars, 2026-09
    91/100 stars
    		  Buy from Supplier
    e 64 inhibitor 67  (Valiant Co Ltd)
    91
    Valiant Co Ltd e 64 inhibitor 67
    hnf4 is required for blood feeding a , Representative brightfield micrographs of live control(RNAi) animals or hnf4(RNAi) animals that were cultured in media containing bovine red blood cells. The inset in the right panel shows a magnification of the indicated area of the gut filled with undigested hemoglobin as evidenced by the red pigmentation. The number of parasites similar to the representative micrograph is indicated in the upper right of each panel. b , Pie chart depicting the frequency of different gut pigmentation of animals from a . n = 69 control(RNAi) animals and 69 hnf4(RNAi) animals from three biological replicates. c , Graph of the cysteine protease cathepsin activity of lysates from control(RNAi) animals or hnf4(RNAi) animals as determined by the ability to cleave the fluorogenic substrate, Z-FR-AMC, in the presence of no inhibitor (DMSO), the general cysteine protease inhibitor, <t>E-64,</t> or the cathepsin B-selective inhibitor, CA-074. Data are from three biological replicates each in triplicate. d , Representative micrographs of H&E-stained sections of mouse livers 22 days after transplant with RNAi-treated parasites. No granulomata are present in the livers of mice that received hnf4(RNAi) parasites. The number of sections similar to the representative micrograph is indicated in the upper right each panel. Data are from three recipients from one biological replicate. e , Representative image of DAPI-stained parasites recovered from mice 22 days after transplant with RNAi-treated parasites. The number of parasites grossly similar to the representative micrograph is indicated in the upper right of each panel. Data are from parasites perfused from three separate recipients. Nuclei are pseudo-colored grey. Scale bars, a , 100µm, e , 100µm, f , 1mm. ****, p <0.0001 (Welch’s t-test).
    E 64 Inhibitor 67, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/0215284601/E-64/adem_sandeep_r__2018__magnetic_biosensors_for_the_detection_of_proteases-445-37-43
    Average 91 stars, based on 1 article reviews
    e 64 inhibitor 67 - by Bioz Stars, 2026-09
    91/100 stars
    		  Buy from Supplier
    lysosomal cathepsins e 64  (Valiant Co Ltd)
    91
    Valiant Co Ltd lysosomal cathepsins e 64
    hnf4 is required for blood feeding a , Representative brightfield micrographs of live control(RNAi) animals or hnf4(RNAi) animals that were cultured in media containing bovine red blood cells. The inset in the right panel shows a magnification of the indicated area of the gut filled with undigested hemoglobin as evidenced by the red pigmentation. The number of parasites similar to the representative micrograph is indicated in the upper right of each panel. b , Pie chart depicting the frequency of different gut pigmentation of animals from a . n = 69 control(RNAi) animals and 69 hnf4(RNAi) animals from three biological replicates. c , Graph of the cysteine protease cathepsin activity of lysates from control(RNAi) animals or hnf4(RNAi) animals as determined by the ability to cleave the fluorogenic substrate, Z-FR-AMC, in the presence of no inhibitor (DMSO), the general cysteine protease inhibitor, <t>E-64,</t> or the cathepsin B-selective inhibitor, CA-074. Data are from three biological replicates each in triplicate. d , Representative micrographs of H&E-stained sections of mouse livers 22 days after transplant with RNAi-treated parasites. No granulomata are present in the livers of mice that received hnf4(RNAi) parasites. The number of sections similar to the representative micrograph is indicated in the upper right each panel. Data are from three recipients from one biological replicate. e , Representative image of DAPI-stained parasites recovered from mice 22 days after transplant with RNAi-treated parasites. The number of parasites grossly similar to the representative micrograph is indicated in the upper right of each panel. Data are from parasites perfused from three separate recipients. Nuclei are pseudo-colored grey. Scale bars, a , 100µm, e , 100µm, f , 1mm. ****, p <0.0001 (Welch’s t-test).
    Lysosomal Cathepsins E 64, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/0215284601/E-64/pm25310712-67-7-12
    Average 91 stars, based on 1 article reviews
    lysosomal cathepsins e 64 - by Bioz Stars, 2026-09
    91/100 stars
    		  Buy from Supplier

    Image Search Results


    hnf4 is required for blood feeding a , Representative brightfield micrographs of live control(RNAi) animals or hnf4(RNAi) animals that were cultured in media containing bovine red blood cells. The inset in the right panel shows a magnification of the indicated area of the gut filled with undigested hemoglobin as evidenced by the red pigmentation. The number of parasites similar to the representative micrograph is indicated in the upper right of each panel. b , Pie chart depicting the frequency of different gut pigmentation of animals from a . n = 69 control(RNAi) animals and 69 hnf4(RNAi) animals from three biological replicates. c , Graph of the cysteine protease cathepsin activity of lysates from control(RNAi) animals or hnf4(RNAi) animals as determined by the ability to cleave the fluorogenic substrate, Z-FR-AMC, in the presence of no inhibitor (DMSO), the general cysteine protease inhibitor, E-64, or the cathepsin B-selective inhibitor, CA-074. Data are from three biological replicates each in triplicate. d , Representative micrographs of H&E-stained sections of mouse livers 22 days after transplant with RNAi-treated parasites. No granulomata are present in the livers of mice that received hnf4(RNAi) parasites. The number of sections similar to the representative micrograph is indicated in the upper right each panel. Data are from three recipients from one biological replicate. e , Representative image of DAPI-stained parasites recovered from mice 22 days after transplant with RNAi-treated parasites. The number of parasites grossly similar to the representative micrograph is indicated in the upper right of each panel. Data are from parasites perfused from three separate recipients. Nuclei are pseudo-colored grey. Scale bars, a , 100µm, e , 100µm, f , 1mm. ****, p <0.0001 (Welch’s t-test).

    Journal: bioRxiv

    Article Title: A single-cell RNAseq atlas of the pathogenic stage of Schistosoma mansoni identifies a key regulator of blood feeding

    doi: 10.1101/2020.02.03.932004

    Figure Lengend Snippet: hnf4 is required for blood feeding a , Representative brightfield micrographs of live control(RNAi) animals or hnf4(RNAi) animals that were cultured in media containing bovine red blood cells. The inset in the right panel shows a magnification of the indicated area of the gut filled with undigested hemoglobin as evidenced by the red pigmentation. The number of parasites similar to the representative micrograph is indicated in the upper right of each panel. b , Pie chart depicting the frequency of different gut pigmentation of animals from a . n = 69 control(RNAi) animals and 69 hnf4(RNAi) animals from three biological replicates. c , Graph of the cysteine protease cathepsin activity of lysates from control(RNAi) animals or hnf4(RNAi) animals as determined by the ability to cleave the fluorogenic substrate, Z-FR-AMC, in the presence of no inhibitor (DMSO), the general cysteine protease inhibitor, E-64, or the cathepsin B-selective inhibitor, CA-074. Data are from three biological replicates each in triplicate. d , Representative micrographs of H&E-stained sections of mouse livers 22 days after transplant with RNAi-treated parasites. No granulomata are present in the livers of mice that received hnf4(RNAi) parasites. The number of sections similar to the representative micrograph is indicated in the upper right each panel. Data are from three recipients from one biological replicate. e , Representative image of DAPI-stained parasites recovered from mice 22 days after transplant with RNAi-treated parasites. The number of parasites grossly similar to the representative micrograph is indicated in the upper right of each panel. Data are from parasites perfused from three separate recipients. Nuclei are pseudo-colored grey. Scale bars, a , 100µm, e , 100µm, f , 1mm. ****, p <0.0001 (Welch’s t-test).

    Article Snippet: Pepstatin A (MP Biomedicals, 0219536805) and E-64 controls were set up by incubating the sample with 10µM of either inhibitor for 30 minutes at room temperature.

    Techniques: Cell Culture, Activity Assay, Protease Inhibitor, Staining

    hnf4 is required for blood feeding a , Graph of the aspartyl protease activity of lysates from control(RNAi) or hnf4(RNAi) parasites as determined by the ability to cleave the fluorogenic substrate, mca-GKPILFFRLK-K(dnp) in the presence of no inhibitor (DMSO), the general cysteine protease inhibitor E-64 (E-64), or the aspartyl protease inhibitor pepstatin A (pepstatin). b , Graph quantifying the recovery rate of worms from transplant recipients. Data are from five recipients. c , Representative photographs of livers of mice 30 days after transplant with RNAi-treated parasites. The number of livers grossly similar to the representative photograph is indicated in the upper right each panel. Data are from two recipients in one biological replicate. d , Graph showing quantification of worm length from . n = 15 for control(RNAi) male parasites and 16 hnf4(RNAi) male parasites from 3 separate recipients. Scale bar, c , 1cm. ns, not significant, ****, p <0.0001 (Welch’s t-test).

    Journal: bioRxiv

    Article Title: A single-cell RNAseq atlas of the pathogenic stage of Schistosoma mansoni identifies a key regulator of blood feeding

    doi: 10.1101/2020.02.03.932004

    Figure Lengend Snippet: hnf4 is required for blood feeding a , Graph of the aspartyl protease activity of lysates from control(RNAi) or hnf4(RNAi) parasites as determined by the ability to cleave the fluorogenic substrate, mca-GKPILFFRLK-K(dnp) in the presence of no inhibitor (DMSO), the general cysteine protease inhibitor E-64 (E-64), or the aspartyl protease inhibitor pepstatin A (pepstatin). b , Graph quantifying the recovery rate of worms from transplant recipients. Data are from five recipients. c , Representative photographs of livers of mice 30 days after transplant with RNAi-treated parasites. The number of livers grossly similar to the representative photograph is indicated in the upper right each panel. Data are from two recipients in one biological replicate. d , Graph showing quantification of worm length from . n = 15 for control(RNAi) male parasites and 16 hnf4(RNAi) male parasites from 3 separate recipients. Scale bar, c , 1cm. ns, not significant, ****, p <0.0001 (Welch’s t-test).

    Article Snippet: Pepstatin A (MP Biomedicals, 0219536805) and E-64 controls were set up by incubating the sample with 10µM of either inhibitor for 30 minutes at room temperature.

    Techniques: Activity Assay, Protease Inhibitor