sodium bisulfite (Valiant Co Ltd)

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Valiant Co Ltd sodium bisulfite
Sodium Bisulfite, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0215257380/Sodium+bisulfite/10__1158_slash_1078___0432__ccr___07___1193-108-23-39
Average 91 stars, based on 12 article reviews

sodium bisulfite - by Bioz Stars, 2026-09

91/100 stars

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Purification:

Article Title: Arms race between anti‐silencing and RdDM in noncoding regions of transposable elements
Article Snippet: For bisulfite sequencing, 300 ng of genomic DNA isolated with Nucleon PhytoPure (GE Healthcare) was digested by Pst I in a 20 μl reaction mix. .. Digested DNA was ethanol‐precipitated and eluted with 20 μl distilled water and then denatured by adding 2.2 μl 3 M NaOH and kept at 37°C for 30 min. Denatured DNA was treated with 208 μl urea/bisulfite solution (7.5 g urea and 7.6 g sodium bisulfite dissolved in 20 ml water, adjusted to pH 5.0) and 12 μl 10 mM hydroquinone followed by 30 cycles of 95°C for 30 s and 55°C for 15 min. Bisulfite‐treated DNA was purified by a GeneClean III Kit (MP Biomedicals), eluted with 20 μl distilled water, and then desulfonated by adding 2.2 μl 3 M NaOH and incubating at 37°C for 15 min. ..

Article Title: Arms race between anti-silencing and RdDM in noncoding regions of transposable elements.
Article Snippet: For bisulfite sequencing, 300 ng of genomic DNA isolated with Nucleon PhytoPure (GE Healthcare) was digested by Pst I in a 20 ll reaction mix. .. Digested DNA was ethanol-precipitated and eluted with 20 ll distilled water and then denatured by adding 2.2 ll 3 M NaOH and kept at 37°C for 30 min. Denatured DNA was treated with 208 ll urea/bisulfite solution (7.5 g urea and 7.6 g sodium bisulfite dissolved in 20 ml water, adjusted to pH 5.0) and 12 ll 10 mM hydroquinone followed by 30 cycles of 95°C for 30 s and 55°C for 15 min. Bisulfite-treated DNA was purified by a GeneClean III Kit (MP Biomedicals), eluted with 20 ll distilled water, and then desulfonated by adding 2.2 ll 3 M NaOH and incubating at 37°C for 15 min. ..

In Situ:

Article Title: Matrix-metalloproteinase expression and gelatinase activity in the avian retina and their influence on Müller glia proliferation
Article Snippet: In situ zymography staining Retinal tissue was fixed for 48hrs at room temperature (RT) in zinc-based fixative consisting of 36.7mM ZnCl 2 (Sigma-Aldrich, St. Louis, MO), 27.3mM ZnAc 2 × 2H 2 O (MP Biomedicals, Santa Ana, CA), and 0.63mM CaAc 2 (Spectrum Chemical MFG Corp, New Brunswick, NJ) dissolved in 0.1M Tris-HCl (Sigma-Aldrich), pH 7.4. .. In situ zymography staining Retinal tissue was fixed for 48hrs at room temperature (RT) in zinc-based fixative consisting of 36.7mM ZnCl 2 (Sigma-Aldrich, St. Louis, MO), 27.3mM ZnAc 2 × 2H 2 O (MP Biomedicals, Santa Ana, CA), and 0.63mM CaAc 2 (Spectrum Chemical MFG Corp, New Brunswick, NJ) dissolved in 0.1M Tris-HCl (Sigma-Aldrich), pH 7.4. .. Sections were then incubated in a humidity chamber at 37°C for 1hr with either 200μM 1,10-Phenanthroline (Sigma-Aldrich) diluted in water (MMP inhibitor control), or at 4°C for 1hr (temperature control).

Zymography:

Staining:

other:

Article Title: Macrophage Effects on Mesenchymal Stem Cell Osteogenesis in a Three-Dimensional In Vitro Bone Model
Article Snippet: The scaffolds were cultured in a co-culture medium (DMEM, 10% FBS, 1% antibiotic and antimycotic solution, 50 μM L-ascorbic acid (Sigma-Aldrich), 100 nM dexamethasone (Sigma-Aldrich, added from day 0 to day 14), 10 mM β-glycerophosphate (MP Biomedicals), 100 mM Vitamin D3 (Sigma-Aldrich), 100 ng/ml BMP-7 (PeproTech), and 50 ng/ml of M-CSF) for 4 weeks.

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