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lactoferrin igg for capture  (Valiant Co Ltd)

 
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    Structured Review

    Valiant Co Ltd lactoferrin igg for capture
    Figure 2 <t>Lactoferrin</t> (A) and polymeric mucin (B) levels in BAL fluid samples from children with CF or other disorders (non-CF disease controls). (A) Lactoferrin concentra- tions were increased in CF BAL samples as compared with those in disease con- trol samples. In the non-CF disease controls, MUC5B was the dominant secreted mucin. MUC5B levels were reduced in CF versus normal samples when mucin quantitation was determined by immunodetection. MUC5AC levels were not signifi- cantly lower in CF samples. Data represent the mean ± SEM (*P < 0.01).
    Lactoferrin Igg For Capture, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 91/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/0215233310/Lactoferrin/10__1172_slash_jci73469-276-15-27
    Average 91 stars, based on 30 article reviews
    lactoferrin igg for capture - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "Cystic fibrosis airway secretions exhibit mucin hyperconcentration and increased osmotic pressure"

    Article Title: Cystic fibrosis airway secretions exhibit mucin hyperconcentration and increased osmotic pressure

    Journal: Journal of Clinical Investigation

    doi: 10.1172/jci73469

    Figure 2 Lactoferrin (A) and polymeric mucin (B) levels in BAL fluid samples from children with CF or other disorders (non-CF disease controls). (A) Lactoferrin concentra- tions were increased in CF BAL samples as compared with those in disease con- trol samples. In the non-CF disease controls, MUC5B was the dominant secreted mucin. MUC5B levels were reduced in CF versus normal samples when mucin quantitation was determined by immunodetection. MUC5AC levels were not signifi- cantly lower in CF samples. Data represent the mean ± SEM (*P < 0.01).
    Figure Legend Snippet: Figure 2 Lactoferrin (A) and polymeric mucin (B) levels in BAL fluid samples from children with CF or other disorders (non-CF disease controls). (A) Lactoferrin concentra- tions were increased in CF BAL samples as compared with those in disease con- trol samples. In the non-CF disease controls, MUC5B was the dominant secreted mucin. MUC5B levels were reduced in CF versus normal samples when mucin quantitation was determined by immunodetection. MUC5AC levels were not signifi- cantly lower in CF samples. Data represent the mean ± SEM (*P < 0.01).

    Techniques Used: Quantitation Assay, Immunodetection

    Related Articles

    Concentration Assay:

    Article Title: Differential Functional Responses of Neutrophil Subsets in Severe COVID-19 Patients
    Article Snippet: .. After fixation with paraformaldehyde (2% final concentration) for 30 min at room temperature, cells were blocked in 1% BSA/PBS for 1h at room temperature, followed by incubation with anti-lactoferrin antibody (MP Biomedicals #55040; 1:500), in 1% BSA/PBS, overnight at 4°C. .. Primary antibody was washed off with three 5 min PBS washes, followed by incubation with donkey anti-rabbit Alexa-Fluor 546 (Thermo Fisher #A10040; 1:1000) in 1% BSA/PBS for 1h at room temperature.

    Incubation:

    Article Title: Differential Functional Responses of Neutrophil Subsets in Severe COVID-19 Patients
    Article Snippet: .. After fixation with paraformaldehyde (2% final concentration) for 30 min at room temperature, cells were blocked in 1% BSA/PBS for 1h at room temperature, followed by incubation with anti-lactoferrin antibody (MP Biomedicals #55040; 1:500), in 1% BSA/PBS, overnight at 4°C. .. Primary antibody was washed off with three 5 min PBS washes, followed by incubation with donkey anti-rabbit Alexa-Fluor 546 (Thermo Fisher #A10040; 1:1000) in 1% BSA/PBS for 1h at room temperature.

    Article Title: Antagonizing FcαR1 (CD89) as treatment in IgA-mediated chronic inflammation and autoimmunity.
    Article Snippet: Briefly, MaxisorpTM plates (Nunc Maxisorp) were coated with 50 μg/ml polyclonal rabbit-anti-human-lactoferrin antibodies (Sigma, L3262) O/N at 4°C and blocked with PBS/0.005% Tween-20/0.5% BSA 1 h at 37°C. .. Two times diluted supernatants were added to plates and incubated for 1h at 37°C followed by incubation with alkaline phosphatase-labeled rabbit-anti-human-lactoferrin antibodies (1:2500, MP Biomedicals) for 1h at 37°C. .. After adding the chromogenic substrate P-nitrophenyl phosphate (Sigma), optical density was measured with a microplate reader (Biorad) at 405 nm.

    Modification:

    Article Title: Impact of Chronic Exposure to Arsenate through Drinking Water on the Intestinal Barrier.
    Article Snippet: .. For lactoferrin analysis, feces (50 mg) were homogenized at a 1:10 proportion in modified RIPA buffer with a bead beater (FastPrep-24 5G Instrument, MP Biomedical), using 0.1 mm glass beads and three cycles of 40 s at a 6 m/s speed, with 1 min intervals in which samples were kept on ice. .. The fecal contents of lactoferrin in the homogenates were analyzed using a specific ELISA kit (MyBioSource).

    Article Title: Impact of Chronic Exposure to Arsenate through Drinking Water on the Intestinal Barrier
    Article Snippet: .. For lactoferrin analysis, feces (50 mg) were homogenized at a 1:10 proportion in modified RIPA buffer with a bead beater (FastPrep-24 5G Instrument, MP Biomedical), using 0.1 mm glass beads and three cycles of 40 s at a 6 m/s speed, with 1 min intervals in which samples were kept on ice. .. The fecal contents of lactoferrin in the homogenates were analyzed using a specific ELISA kit (MyBioSource).



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    Differential degranulation by neutrophil subsets from COVID-19 patients. (A) Basal and stimulated (TNFα 2 ng/ml + fMLF 300 nM) expression of markers for secretory vesicles (CD35), gelatinase granules (CD11b), specific granules (CD66b), and azurophilic granules (CD63) on COVID NDN (n=11-15), COVID LDN (n=11-15), and HD NDN (n=8-9). Results are expressed as mean ± SEM of mean fluorescent intensity (MFI). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. (B) Comparison of basal expression of CD35, CD11b, CD66b, and CD63 on COVID NDN, CD16 Hi LDN, and CD16 Int LDN (n=7). Results are expressed as mean ± SEM of MFI. *p < 0.05, **p < 0.01, ***p < 0.001. (C) Plasma levels of MMP9, <t>lactoferrin,</t> and human neutrophil elastase in plasma from HD (n=10) and COVID-19 (n=10-25). Results expressed as mean + SEM in ng/ml. *p < 0.05, **p < 0.01, ***p < 0.001.
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    ( A ) Volcano plot shows differentially expressed genes (DEGs) between CD16 Int and CD16 hi LDN. ( B ) Top 20 enriched gene ontology biological pathway categories for CD16 Int versus CD16 hi LDNs from severe COVID-19 patients. ( C ) The heatmap shows DEGs related to neutrophil degranulation and NET formation, neutrophil phagocytosis, neutrophil signaling, and neutrophil trafficking and function between CD16 hi and CD16 Int LDNs. ( D ) The phagocytic capacity of CD16 hi and CD16 Int neutrophils from whole blood in severe COVID-19 patients ( n = 4) was assessed using a pHrodo Green S . aureus BioParticles phagocytosis assay. Gating strategy, representative histogram, and summarized MFI data are shown. ** P < 0.01 (Student’s t test). ( E ) Representative confocal image of spontaneous NET formation from sorted CD16 Int LDNs. Anti-human <t>lactoferrin</t> (shown in red) and neutrophil DNA stained with DAPI (shown in blue) merge image shows NET characteristic structures. Original magnification, ×60. Data are presented as mean ± SD.
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    Image Search Results


    Differential degranulation by neutrophil subsets from COVID-19 patients. (A) Basal and stimulated (TNFα 2 ng/ml + fMLF 300 nM) expression of markers for secretory vesicles (CD35), gelatinase granules (CD11b), specific granules (CD66b), and azurophilic granules (CD63) on COVID NDN (n=11-15), COVID LDN (n=11-15), and HD NDN (n=8-9). Results are expressed as mean ± SEM of mean fluorescent intensity (MFI). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. (B) Comparison of basal expression of CD35, CD11b, CD66b, and CD63 on COVID NDN, CD16 Hi LDN, and CD16 Int LDN (n=7). Results are expressed as mean ± SEM of MFI. *p < 0.05, **p < 0.01, ***p < 0.001. (C) Plasma levels of MMP9, lactoferrin, and human neutrophil elastase in plasma from HD (n=10) and COVID-19 (n=10-25). Results expressed as mean + SEM in ng/ml. *p < 0.05, **p < 0.01, ***p < 0.001.

    Journal: Frontiers in Immunology

    Article Title: Differential Functional Responses of Neutrophil Subsets in Severe COVID-19 Patients

    doi: 10.3389/fimmu.2022.879686

    Figure Lengend Snippet: Differential degranulation by neutrophil subsets from COVID-19 patients. (A) Basal and stimulated (TNFα 2 ng/ml + fMLF 300 nM) expression of markers for secretory vesicles (CD35), gelatinase granules (CD11b), specific granules (CD66b), and azurophilic granules (CD63) on COVID NDN (n=11-15), COVID LDN (n=11-15), and HD NDN (n=8-9). Results are expressed as mean ± SEM of mean fluorescent intensity (MFI). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. (B) Comparison of basal expression of CD35, CD11b, CD66b, and CD63 on COVID NDN, CD16 Hi LDN, and CD16 Int LDN (n=7). Results are expressed as mean ± SEM of MFI. *p < 0.05, **p < 0.01, ***p < 0.001. (C) Plasma levels of MMP9, lactoferrin, and human neutrophil elastase in plasma from HD (n=10) and COVID-19 (n=10-25). Results expressed as mean + SEM in ng/ml. *p < 0.05, **p < 0.01, ***p < 0.001.

    Article Snippet: After fixation with paraformaldehyde (2% final concentration) for 30 min at room temperature, cells were blocked in 1% BSA/PBS for 1h at room temperature, followed by incubation with anti-lactoferrin antibody (MP Biomedicals #55040; 1:500), in 1% BSA/PBS, overnight at 4°C.

    Techniques: Expressing, Comparison

    Enhanced NET formation by COVID-19 LDN. Panel (A) shows merged confocal images of lactoferrin staining of NETs (Red) and DAPI staining of DNA (Blue), demonstrating NET formation, in the absence (Basal) or presence of CFSE-labelled P. stomatis , in HD NDN, COVID NDN, and COVID LDN, representative of 4 experiments. Images shown are stacks of the entire collection of acquired z-planes at 20x magnification, scale bar=100 mm. Individual images are shown in Supplemental Figures 1 and 2 . Panel (B) shows release of dsDNA into supernatant from basal or P. stomatis stimulated HD NDN, COVID NDN, and COVID LDN. NET formation by COVID LDN was significantly greater following bacterial stimulation, compared to COVID NDN and HD NDN, n=12-15. *p < 0.05, **p < 0.01. Panel (C) shows the results of 3 separate experiments comparing the percent of CD16 Hi LDN and CD16 Int LDN spontaneously forming NETs, visualized by confocal microscopy.

    Journal: Frontiers in Immunology

    Article Title: Differential Functional Responses of Neutrophil Subsets in Severe COVID-19 Patients

    doi: 10.3389/fimmu.2022.879686

    Figure Lengend Snippet: Enhanced NET formation by COVID-19 LDN. Panel (A) shows merged confocal images of lactoferrin staining of NETs (Red) and DAPI staining of DNA (Blue), demonstrating NET formation, in the absence (Basal) or presence of CFSE-labelled P. stomatis , in HD NDN, COVID NDN, and COVID LDN, representative of 4 experiments. Images shown are stacks of the entire collection of acquired z-planes at 20x magnification, scale bar=100 mm. Individual images are shown in Supplemental Figures 1 and 2 . Panel (B) shows release of dsDNA into supernatant from basal or P. stomatis stimulated HD NDN, COVID NDN, and COVID LDN. NET formation by COVID LDN was significantly greater following bacterial stimulation, compared to COVID NDN and HD NDN, n=12-15. *p < 0.05, **p < 0.01. Panel (C) shows the results of 3 separate experiments comparing the percent of CD16 Hi LDN and CD16 Int LDN spontaneously forming NETs, visualized by confocal microscopy.

    Article Snippet: After fixation with paraformaldehyde (2% final concentration) for 30 min at room temperature, cells were blocked in 1% BSA/PBS for 1h at room temperature, followed by incubation with anti-lactoferrin antibody (MP Biomedicals #55040; 1:500), in 1% BSA/PBS, overnight at 4°C.

    Techniques: Staining, Confocal Microscopy

    ( A ) Volcano plot shows differentially expressed genes (DEGs) between CD16 Int and CD16 hi LDN. ( B ) Top 20 enriched gene ontology biological pathway categories for CD16 Int versus CD16 hi LDNs from severe COVID-19 patients. ( C ) The heatmap shows DEGs related to neutrophil degranulation and NET formation, neutrophil phagocytosis, neutrophil signaling, and neutrophil trafficking and function between CD16 hi and CD16 Int LDNs. ( D ) The phagocytic capacity of CD16 hi and CD16 Int neutrophils from whole blood in severe COVID-19 patients ( n = 4) was assessed using a pHrodo Green S . aureus BioParticles phagocytosis assay. Gating strategy, representative histogram, and summarized MFI data are shown. ** P < 0.01 (Student’s t test). ( E ) Representative confocal image of spontaneous NET formation from sorted CD16 Int LDNs. Anti-human lactoferrin (shown in red) and neutrophil DNA stained with DAPI (shown in blue) merge image shows NET characteristic structures. Original magnification, ×60. Data are presented as mean ± SD.

    Journal: JCI Insight

    Article Title: A specific low-density neutrophil population correlates with hypercoagulation and disease severity in hospitalized COVID-19 patients

    doi: 10.1172/jci.insight.148435

    Figure Lengend Snippet: ( A ) Volcano plot shows differentially expressed genes (DEGs) between CD16 Int and CD16 hi LDN. ( B ) Top 20 enriched gene ontology biological pathway categories for CD16 Int versus CD16 hi LDNs from severe COVID-19 patients. ( C ) The heatmap shows DEGs related to neutrophil degranulation and NET formation, neutrophil phagocytosis, neutrophil signaling, and neutrophil trafficking and function between CD16 hi and CD16 Int LDNs. ( D ) The phagocytic capacity of CD16 hi and CD16 Int neutrophils from whole blood in severe COVID-19 patients ( n = 4) was assessed using a pHrodo Green S . aureus BioParticles phagocytosis assay. Gating strategy, representative histogram, and summarized MFI data are shown. ** P < 0.01 (Student’s t test). ( E ) Representative confocal image of spontaneous NET formation from sorted CD16 Int LDNs. Anti-human lactoferrin (shown in red) and neutrophil DNA stained with DAPI (shown in blue) merge image shows NET characteristic structures. Original magnification, ×60. Data are presented as mean ± SD.

    Article Snippet: NETs were determined by extracellular colocalization of anti-human lactoferrin antibody (1:500 dilution, MP Biomedicals, catalog 55040) with DAPI (600 nM for 10 minutes) nuclear stain.

    Techniques: Phagocytosis Assay, Staining