sodium nitroprusside  (Valiant Co Ltd)

Journal: bioRxiv

Article Title: Micro and nanoplastic inhalation during pregnancy elicits uterine endothelial dysfunction in Sprague Dawley rats by impeding nitric oxide signaling

doi: 10.1101/2025.09.12.675811

Figure Lengend Snippet: Vascular reactivity of the uterine artery was assessed via wire myography. Concentration-response curves were generated after treatment of uterine artery segments with increasing concentrations of the endothelial-dependent vasodilator methacholine (A), the endothelial-independent vasodilator sodium nitroprusside (B), and the vasoconstrictor phenylephrine (C). Significance was assessed by comparing overall reactivity via a four-parameter nonlinear regression analysis. Data are presented as mean ± SEM, n =7-10.

Article Snippet: Endothelium-dependent relaxation, endothelium-independent relaxation, and smooth muscle contraction were separately evaluated via cumulative additions of 60 μL of Methacholine chloride (MCh, MP Biomedicals, CAT 190231), Sodium Nitroprusside Dihydrate (SNP, Sigma-Aldrich, CAT 567538), and Phenylephrine (PE, Thermofisher Scientific, CAT 207240100), respectively for a concentration-response curve spanning from 10 − 9 to 10 − 4 M for each pharmacological application.

Techniques: Concentration Assay, Generated

Journal: bioRxiv

Article Title: Micro and nanoplastic inhalation during pregnancy elicits uterine endothelial dysfunction in Sprague Dawley rats by impeding nitric oxide signaling

doi: 10.1101/2025.09.12.675811

Figure Lengend Snippet: Vascular reactivity of the radial artery was assessed via pressure myography. Concentration-response curves were generated after treatment of radial artery segments with increasing concentrations of the endothelial-dependent vasodilator methacholine (A), the endothelial-independent vasodilator sodium nitroprusside (B), and the vasoconstrictor phenylephrine (C). Significance was assessed by comparing overall reactivity via a four-parameter nonlinear regression analysis. Data are presented as mean ± SEM, n =8-10. Significant differences (p < 0.05) from the control group are denoted by * and significant differences from the polyamide-exposed group are denoted by †.

Article Snippet: Endothelium-dependent relaxation, endothelium-independent relaxation, and smooth muscle contraction were separately evaluated via cumulative additions of 60 μL of Methacholine chloride (MCh, MP Biomedicals, CAT 190231), Sodium Nitroprusside Dihydrate (SNP, Sigma-Aldrich, CAT 567538), and Phenylephrine (PE, Thermofisher Scientific, CAT 207240100), respectively for a concentration-response curve spanning from 10 − 9 to 10 − 4 M for each pharmacological application.

Techniques: Concentration Assay, Generated, Control

Journal: bioRxiv

Article Title: Chronic Pod-Mod E-Cigarette Aerosol Exposure Induces Aortic Dysfunction in Hypercholesterolemic Mice: Role of Oxidative Stress and Inflammation

doi: 10.1101/2024.01.30.578110

Figure Lengend Snippet: The percent recovery of outer diameter from vasoconstriction by submaximal phenylephrine dose and in response to increasing concentrations of sodium nitroprusside (NO donor) measures the endothelium-independent vasodilatation (A). The percent recovery of outer diameter from vasoconstriction by submaximal phenylephrine dose and in response to increasing concentrations of acetylcholine measures the endothelium-dependent vasodilatation (B, circles). Incubation of aortic samples with L-NAME (NOS inhibitor) ahead of phenylephrine vasoconstriction and acetylcholine treatment quantifies the portion of endothelium-dependent vasodilatation that does not rely upon NO release (B, diamond). The difference between the dilatation to the largest acetylcholine dose without and with L-NAME provides an indirect estimate of NO availability in aortic tissues (C). Expression of eNOS in endothelial cells (E) from immunostaining (red) and colocalization with Pecam1 (green) in aortic cross-sections (D). mRNA expression of the Nos3 gene coding for eNOS in whole aorta tissue samples (F). Chronic exposure to pod-mod e-cigarette aerosol hindered the endothelium-dependent vasodilatation of the aortic wall via abrogation of the NO-mediated response. Statistical significance denoted by * for p < 0.05 or ** for p < 0.01 in näıve e-cigarette vs . air control, † for p < 0.05 in L-NAME pre-treated vs . näıve in air control, and § for p < 0.05 in L-NAME pre-treated vs . näıve in e-cigarette.

Article Snippet: Endothelium-independent vasodilatation was finally probed by administering increasing concentrations between 10 − 9 and 10 − 4 M of the nitric oxide (NO) donor sodium nitroprusside (MP Biomedicals; Irvine, CA, USA) through the lumen of pressurized, axially extended, and pre-constricted aortic samples.

Techniques: Incubation, Expressing, Immunostaining, Aerosol