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Valiant Co Ltd 3 methylxanthine
(two columns) Engineering methylxanthine production using an alternate pathway. The top molecular structure illustrates methyltransferases used in the study and their corresponding N-methylation position on the xanthine backbone. The plot below represents the titer of four different methylxanthines produced in the media of the engineered strains, optimized for 1-, <t>3-methylxanthine</t> and theophylline production (grey) and for 3-methylxanthine production (light blue). Production of methylxanthines was analyzed by LC–MS/MS and data are reported as the mean and standard deviation of biological triplicates. Relevant genetic contents for each strain are displayed next to the vertical bar on the left of the plot (see Table 1 for complete strain information).
3 Methylxanthine, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0215168680/3-Methylxanthine/pmc05107131-134-0-6
Average 93 stars, based on 11 article reviews
3 methylxanthine - by Bioz Stars, 2026-09
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1) Product Images from "Engineering a microbial platform for de novo biosynthesis of diverse methylxanthines"

Article Title: Engineering a microbial platform for de novo biosynthesis of diverse methylxanthines

Journal: Metabolic engineering

doi: 10.1016/j.ymben.2016.08.003

(two columns) Engineering methylxanthine production using an alternate pathway. The top molecular structure illustrates methyltransferases used in the study and their corresponding N-methylation position on the xanthine backbone. The plot below represents the titer of four different methylxanthines produced in the media of the engineered strains, optimized for 1-, 3-methylxanthine and theophylline production (grey) and for 3-methylxanthine production (light blue). Production of methylxanthines was analyzed by LC–MS/MS and data are reported as the mean and standard deviation of biological triplicates. Relevant genetic contents for each strain are displayed next to the vertical bar on the left of the plot (see Table 1 for complete strain information).
Figure Legend Snippet: (two columns) Engineering methylxanthine production using an alternate pathway. The top molecular structure illustrates methyltransferases used in the study and their corresponding N-methylation position on the xanthine backbone. The plot below represents the titer of four different methylxanthines produced in the media of the engineered strains, optimized for 1-, 3-methylxanthine and theophylline production (grey) and for 3-methylxanthine production (light blue). Production of methylxanthines was analyzed by LC–MS/MS and data are reported as the mean and standard deviation of biological triplicates. Relevant genetic contents for each strain are displayed next to the vertical bar on the left of the plot (see Table 1 for complete strain information).

Techniques Used: Methylation, Produced, Liquid Chromatography with Mass Spectroscopy, Standard Deviation

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Cell Culture:

Article Title: Characterization and utility of two monoclonal antibodies to cholera toxin B subunit.
Article Snippet: .. Caco2 cells were grown in 12-well cluster plates (Thermo Scientific Nunc Cell-Culture Treated, Roskilde, Denmark) at a density of 7 × 105 Caco2 cells/well containing EMEM (Gibco BRL) medium supplemented with 20% FBS, 5 mM HEPES, 5 mM NEAA, 5 mM Sodium Pyruvate and Penicillin/Streptomycin for 24 h. The culture medium was removed, and the cells were washed twice with PBS and then incubated in serum-free EMEM medium containing 2.5 mM HEPES, 0.01% bovine serum albumin, 1 mM 3-Isobutyl-1-methylxanthine (MP Biomedicals, LLC, Solon, Ohio), and the indicated concentrations of 7A12B3 and 9F9C7 mAbs, and 0.5 μg/mL (5.68 nM) of CTX (Sigma, St. Louis, Mo) or rat IgG isotype control for 30 min at 4 °C (on ice). .. The plates were subsequently transferred to 37 °C in a 5% CO2 incubator for 2 h. The culture plates were centrifuged at 1000×g, the culture medium removed, and the cells washed twice with PBS before incubation in 200 μL of 0.1 N HCl for 20 min at room temperature to allow cell lysis and cAMP extraction32. cAMP was detected using a sensitive colorimetric ELISA-based kit (Enzo Life Sciences, Farmingdale, NY) following the manufacturer’s conditions.

Article Title: Characterization and utility of two monoclonal antibodies to cholera toxin B subunit
Article Snippet: The data were fit to a Bivalent model using the global data analysis option available within Biacore Evaluation software. .. Caco2 cells were grown in 12-well cluster plates (Thermo Scientific Nunc Cell-Culture Treated, Roskilde, Denmark) at a density of 7 × 10 5 Caco2 cells/well containing EMEM (Gibco BRL) medium supplemented with 20% FBS, 5 mM HEPES, 5 mM NEAA, 5 mM Sodium Pyruvate and Penicillin/Streptomycin for 24 h. The culture medium was removed, and the cells were washed twice with PBS and then incubated in serum-free EMEM medium containing 2.5 mM HEPES, 0.01% bovine serum albumin, 1 mM 3-Isobutyl-1-methylxanthine (MP Biomedicals, LLC, Solon, Ohio), and the indicated concentrations of 7A12B3 and 9F9C7 mAbs, and 0.5 μg/mL (5.68 nM) of CTX (Sigma, St. Louis, Mo) or rat IgG isotype control for 30 min at 4 °C (on ice). .. The plates were subsequently transferred to 37 °C in a 5% CO 2 incubator for 2 h. The culture plates were centrifuged at 1000× g , the culture medium removed, and the cells washed twice with PBS before incubation in 200 μL of 0.1 N HCl for 20 min at room temperature to allow cell lysis and cAMP extraction . cAMP was detected using a sensitive colorimetric ELISA-based kit (Enzo Life Sciences, Farmingdale, NY) following the manufacturer’s conditions.

Incubation:

Article Title: Characterization and utility of two monoclonal antibodies to cholera toxin B subunit.
Article Snippet: .. Caco2 cells were grown in 12-well cluster plates (Thermo Scientific Nunc Cell-Culture Treated, Roskilde, Denmark) at a density of 7 × 105 Caco2 cells/well containing EMEM (Gibco BRL) medium supplemented with 20% FBS, 5 mM HEPES, 5 mM NEAA, 5 mM Sodium Pyruvate and Penicillin/Streptomycin for 24 h. The culture medium was removed, and the cells were washed twice with PBS and then incubated in serum-free EMEM medium containing 2.5 mM HEPES, 0.01% bovine serum albumin, 1 mM 3-Isobutyl-1-methylxanthine (MP Biomedicals, LLC, Solon, Ohio), and the indicated concentrations of 7A12B3 and 9F9C7 mAbs, and 0.5 μg/mL (5.68 nM) of CTX (Sigma, St. Louis, Mo) or rat IgG isotype control for 30 min at 4 °C (on ice). .. The plates were subsequently transferred to 37 °C in a 5% CO2 incubator for 2 h. The culture plates were centrifuged at 1000×g, the culture medium removed, and the cells washed twice with PBS before incubation in 200 μL of 0.1 N HCl for 20 min at room temperature to allow cell lysis and cAMP extraction32. cAMP was detected using a sensitive colorimetric ELISA-based kit (Enzo Life Sciences, Farmingdale, NY) following the manufacturer’s conditions.

Article Title: Characterization and utility of two monoclonal antibodies to cholera toxin B subunit
Article Snippet: The data were fit to a Bivalent model using the global data analysis option available within Biacore Evaluation software. .. Caco2 cells were grown in 12-well cluster plates (Thermo Scientific Nunc Cell-Culture Treated, Roskilde, Denmark) at a density of 7 × 10 5 Caco2 cells/well containing EMEM (Gibco BRL) medium supplemented with 20% FBS, 5 mM HEPES, 5 mM NEAA, 5 mM Sodium Pyruvate and Penicillin/Streptomycin for 24 h. The culture medium was removed, and the cells were washed twice with PBS and then incubated in serum-free EMEM medium containing 2.5 mM HEPES, 0.01% bovine serum albumin, 1 mM 3-Isobutyl-1-methylxanthine (MP Biomedicals, LLC, Solon, Ohio), and the indicated concentrations of 7A12B3 and 9F9C7 mAbs, and 0.5 μg/mL (5.68 nM) of CTX (Sigma, St. Louis, Mo) or rat IgG isotype control for 30 min at 4 °C (on ice). .. The plates were subsequently transferred to 37 °C in a 5% CO 2 incubator for 2 h. The culture plates were centrifuged at 1000× g , the culture medium removed, and the cells washed twice with PBS before incubation in 200 μL of 0.1 N HCl for 20 min at room temperature to allow cell lysis and cAMP extraction . cAMP was detected using a sensitive colorimetric ELISA-based kit (Enzo Life Sciences, Farmingdale, NY) following the manufacturer’s conditions.

Article Title: Characterization and utility of novel monoclonal antibodies to cholera toxin B subunit
Article Snippet: Intracellular cAMP in Caco2 cell line Caco2 cells were grown in 6-wells and 12-wells cluster plates (Thermo Scienti c Nunc Cell-Culture Treated, Roskilde, Denmark) at a density of 7x105 Caco2 cells/well containing EMEM (Gibco BRL) medium supplemented with 20% FBS, 5 mM HEPES, 5 mM NEAA, 5 mM Sodium Pyruvate and Penicillin/Streptomycin for 24 hr. .. The culture medium was removed, and the cells were washed twice with PBS and then incubated in serum-free EMEM medium containing 2.5 mM HEPES, 0.01% bovine serum albumin, 1 mM 3-Isobutyl-1-methylxanthine (MP Biomedicals, LLC, Solon, Ohio), and the indicated concentrations of 7A12B3 and 9F9C7 mAbs, and 0.5 μg/mL (5.88 pmoles) of CTX (Sigma, St. Louis, Mo) at 4°C (on ice) or rat IgG isotype control for 30 min. ..

Control:

Article Title: Characterization and utility of two monoclonal antibodies to cholera toxin B subunit.
Article Snippet: .. Caco2 cells were grown in 12-well cluster plates (Thermo Scientific Nunc Cell-Culture Treated, Roskilde, Denmark) at a density of 7 × 105 Caco2 cells/well containing EMEM (Gibco BRL) medium supplemented with 20% FBS, 5 mM HEPES, 5 mM NEAA, 5 mM Sodium Pyruvate and Penicillin/Streptomycin for 24 h. The culture medium was removed, and the cells were washed twice with PBS and then incubated in serum-free EMEM medium containing 2.5 mM HEPES, 0.01% bovine serum albumin, 1 mM 3-Isobutyl-1-methylxanthine (MP Biomedicals, LLC, Solon, Ohio), and the indicated concentrations of 7A12B3 and 9F9C7 mAbs, and 0.5 μg/mL (5.68 nM) of CTX (Sigma, St. Louis, Mo) or rat IgG isotype control for 30 min at 4 °C (on ice). .. The plates were subsequently transferred to 37 °C in a 5% CO2 incubator for 2 h. The culture plates were centrifuged at 1000×g, the culture medium removed, and the cells washed twice with PBS before incubation in 200 μL of 0.1 N HCl for 20 min at room temperature to allow cell lysis and cAMP extraction32. cAMP was detected using a sensitive colorimetric ELISA-based kit (Enzo Life Sciences, Farmingdale, NY) following the manufacturer’s conditions.

Article Title: Characterization and utility of two monoclonal antibodies to cholera toxin B subunit
Article Snippet: The data were fit to a Bivalent model using the global data analysis option available within Biacore Evaluation software. .. Caco2 cells were grown in 12-well cluster plates (Thermo Scientific Nunc Cell-Culture Treated, Roskilde, Denmark) at a density of 7 × 10 5 Caco2 cells/well containing EMEM (Gibco BRL) medium supplemented with 20% FBS, 5 mM HEPES, 5 mM NEAA, 5 mM Sodium Pyruvate and Penicillin/Streptomycin for 24 h. The culture medium was removed, and the cells were washed twice with PBS and then incubated in serum-free EMEM medium containing 2.5 mM HEPES, 0.01% bovine serum albumin, 1 mM 3-Isobutyl-1-methylxanthine (MP Biomedicals, LLC, Solon, Ohio), and the indicated concentrations of 7A12B3 and 9F9C7 mAbs, and 0.5 μg/mL (5.68 nM) of CTX (Sigma, St. Louis, Mo) or rat IgG isotype control for 30 min at 4 °C (on ice). .. The plates were subsequently transferred to 37 °C in a 5% CO 2 incubator for 2 h. The culture plates were centrifuged at 1000× g , the culture medium removed, and the cells washed twice with PBS before incubation in 200 μL of 0.1 N HCl for 20 min at room temperature to allow cell lysis and cAMP extraction . cAMP was detected using a sensitive colorimetric ELISA-based kit (Enzo Life Sciences, Farmingdale, NY) following the manufacturer’s conditions.

Article Title: Characterization and utility of novel monoclonal antibodies to cholera toxin B subunit
Article Snippet: Intracellular cAMP in Caco2 cell line Caco2 cells were grown in 6-wells and 12-wells cluster plates (Thermo Scienti c Nunc Cell-Culture Treated, Roskilde, Denmark) at a density of 7x105 Caco2 cells/well containing EMEM (Gibco BRL) medium supplemented with 20% FBS, 5 mM HEPES, 5 mM NEAA, 5 mM Sodium Pyruvate and Penicillin/Streptomycin for 24 hr. .. The culture medium was removed, and the cells were washed twice with PBS and then incubated in serum-free EMEM medium containing 2.5 mM HEPES, 0.01% bovine serum albumin, 1 mM 3-Isobutyl-1-methylxanthine (MP Biomedicals, LLC, Solon, Ohio), and the indicated concentrations of 7A12B3 and 9F9C7 mAbs, and 0.5 μg/mL (5.88 pmoles) of CTX (Sigma, St. Louis, Mo) at 4°C (on ice) or rat IgG isotype control for 30 min. ..



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(two columns) Engineering methylxanthine production using an alternate pathway. The top molecular structure illustrates methyltransferases used in the study and their corresponding N-methylation position on the xanthine backbone. The plot below represents the titer of four different methylxanthines produced in the media of the engineered strains, optimized for 1-, <t>3-methylxanthine</t> and theophylline production (grey) and for 3-methylxanthine production (light blue). Production of methylxanthines was analyzed by LC–MS/MS and data are reported as the mean and standard deviation of biological triplicates. Relevant genetic contents for each strain are displayed next to the vertical bar on the left of the plot (see Table 1 for complete strain information).
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Image Search Results


(two columns) Engineering methylxanthine production using an alternate pathway. The top molecular structure illustrates methyltransferases used in the study and their corresponding N-methylation position on the xanthine backbone. The plot below represents the titer of four different methylxanthines produced in the media of the engineered strains, optimized for 1-, 3-methylxanthine and theophylline production (grey) and for 3-methylxanthine production (light blue). Production of methylxanthines was analyzed by LC–MS/MS and data are reported as the mean and standard deviation of biological triplicates. Relevant genetic contents for each strain are displayed next to the vertical bar on the left of the plot (see Table 1 for complete strain information).

Journal: Metabolic engineering

Article Title: Engineering a microbial platform for de novo biosynthesis of diverse methylxanthines

doi: 10.1016/j.ymben.2016.08.003

Figure Lengend Snippet: (two columns) Engineering methylxanthine production using an alternate pathway. The top molecular structure illustrates methyltransferases used in the study and their corresponding N-methylation position on the xanthine backbone. The plot below represents the titer of four different methylxanthines produced in the media of the engineered strains, optimized for 1-, 3-methylxanthine and theophylline production (grey) and for 3-methylxanthine production (light blue). Production of methylxanthines was analyzed by LC–MS/MS and data are reported as the mean and standard deviation of biological triplicates. Relevant genetic contents for each strain are displayed next to the vertical bar on the left of the plot (see Table 1 for complete strain information).

Article Snippet: 3-methylxanthine (LOT# 1733J) was obtained from MP Biomedicals (Burlingame, CA).

Techniques: Methylation, Produced, Liquid Chromatography with Mass Spectroscopy, Standard Deviation