dithizone (Valiant Co Ltd)
90
Structured Review
Valiant Co Ltd
dithizone
Dithizone, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0215100001/Dithizone/pmc07228571-118-15-16
Average 90 stars, based on 17 article reviews
Dithizone, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0215100001/Dithizone/pmc07228571-118-15-16
Average 90 stars, based on 17 article reviews
dithizone - by Bioz Stars,
2026-09
90/100 stars
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Cell Characterization:Article Title: Exploring Insulin Production Following Alveolar Islet Transplantation (AIT) Article Snippet: .. To determine islet cell count (IC) and equivalence (IEQ), an aliquot of 100 μL IEQ was collected for staining with 1 mL Staining:Article Title: Exploring Insulin Production Following Alveolar Islet Transplantation (AIT) Article Snippet: .. To determine islet cell count (IC) and equivalence (IEQ), an aliquot of 100 μL IEQ was collected for staining with 1 mL Article Title: Necrostatin-1 Supplementation to Islet Tissue Culture Enhances the In-Vitro Development and Graft Function of Young Porcine Islets Article Snippet: .. A 100 uL aliquot of islets was collected for staining with 1 mL Article Title: The effects of necrostatin-1 on the in vitro development and function of young porcine islets over 14-day prolonged tissue culture. Article Snippet: Funding information Juvenile Diabetes Research Foundation, Grant/Award Number: 17-2013-288; University of California, Irvine; Brownstein Family Foundation; PADRE Foundation Abstract Background: Necrostatin-1 (Nec-1) supplementation to tissue culture media on day 3 has recently been shown to augment the insulin content, endocrine cellular composition, and insulin release of pre-weaned porcine islets (PPIs); however, its effects were only examined for the first 7 days of tissue culture.. The present study examined whether the addition of Nec-1 on day 3 could further enhance the in vitro development and function of PPIs after 14 days of tissue culture.. Methods: PPIs were isolated from 8to 15-day-old, pre-weaned Yorkshire piglets and cultured in an islet maturation media supplemented with Nec-1 on day 3. Article Title: Dose-dependent effects of necrostatin-1 supplementation to tissue culture media of young porcine islets Article Snippet: .. Islet equivalence (IEQ) was acquired by staining 100 μL islet aliquot with 1 mL of Article Title: Characterization of chelator-mediated recovery of pancreatic islets from barium-stabilized alginate microcapsules. Article Snippet: Type I diabetes mellitus (T1DM) is an autoimmune condition charac‐ terized destruction of β‐cells of the pancreatic islet of Langerhans.. This autoimmune‐mediated depletion of β‐cells causes disruptions to insulin secretion and glycemic control homeostasis, which is the driv‐ ing force behind multiple secondary metabolic diseases.1,2 Human pancreatic islet allografts have demonstrated potential therapeutic use to help treat the damaging effects of T1DM.3 The Collaborative Islet Transplant Registry (CITR) has recorded over 1913 recipients of Received: 31 January 2019 | Revised: 29 July 2019 | Accepted: 7 August 2019 DOI: 10.1111/xen.12554 other:Article Title: Necrostatin-1 supplementation enhances young porcine islet maturation and in vitro function. Article Snippet: The advancement in islet transplantation offers a promising cure for patients with type 1 diabetes, especially with the improved islet isolation procedures and the use of a glucocorticoid‐free im‐ munosuppressive protocol.1‐3 Despite these successful outcomes, the large‐scale clinical application of islet transplantation has been limited by a shortage of donor pancreata and the lack of quality is‐ lets after islet isolation.4,5 With the recent progress in gene editing technology and islet xenotransplantation, the use of pancreata from young porcine donors has the potential to serve as an unlimited al‐ ternative source of islets.6‐10 Received: 3 June 2019 | Revised: 13 August 2019 | Accepted: 28 August 2019 DOI: 10.1111/xen.12555 Microscopy:Article Title: Necrostatin-1 Supplementation to Islet Tissue Culture Enhances the In-Vitro Development and Graft Function of Young Porcine Islets Article Snippet: .. A 100 uL aliquot of islets was collected for staining with 1 mL Article Title: The effects of necrostatin-1 on the in vitro development and function of young porcine islets over 14-day prolonged tissue culture. Article Snippet: Funding information Juvenile Diabetes Research Foundation, Grant/Award Number: 17-2013-288; University of California, Irvine; Brownstein Family Foundation; PADRE Foundation Abstract Background: Necrostatin-1 (Nec-1) supplementation to tissue culture media on day 3 has recently been shown to augment the insulin content, endocrine cellular composition, and insulin release of pre-weaned porcine islets (PPIs); however, its effects were only examined for the first 7 days of tissue culture.. The present study examined whether the addition of Nec-1 on day 3 could further enhance the in vitro development and function of PPIs after 14 days of tissue culture.. Methods: PPIs were isolated from 8to 15-day-old, pre-weaned Yorkshire piglets and cultured in an islet maturation media supplemented with Nec-1 on day 3. Article Title: Dose-dependent effects of necrostatin-1 supplementation to tissue culture media of young porcine islets Article Snippet: .. Islet equivalence (IEQ) was acquired by staining 100 μL islet aliquot with 1 mL of Article Title: Characterization of chelator-mediated recovery of pancreatic islets from barium-stabilized alginate microcapsules. Article Snippet: Type I diabetes mellitus (T1DM) is an autoimmune condition charac‐ terized destruction of β‐cells of the pancreatic islet of Langerhans.. This autoimmune‐mediated depletion of β‐cells causes disruptions to insulin secretion and glycemic control homeostasis, which is the driv‐ ing force behind multiple secondary metabolic diseases.1,2 Human pancreatic islet allografts have demonstrated potential therapeutic use to help treat the damaging effects of T1DM.3 The Collaborative Islet Transplant Registry (CITR) has recorded over 1913 recipients of Received: 31 January 2019 | Revised: 29 July 2019 | Accepted: 7 August 2019 DOI: 10.1111/xen.12554 |