m199 (Valiant Co Ltd)

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Valiant Co Ltd m199
M199, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 243 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0215070501/Collagenase/pmc03053052-170-5-18
Average 95 stars, based on 243 article reviews

m199 - by Bioz Stars, 2026-09

95/100 stars

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Article Title: Human Dental Pulp Stem Cells Attenuate Neuroinflammation and Neurological Deficit in a Murine Model of Multiple Sclerosis
Article Snippet: Pulp tissue was extracted and cut into blocks of about 1 mm3, then digested in a solution containing 0.3% collagenase type I (MP Biomedicals LLC, CA, USA) and 0.4% collagenase type II (Sigma- Aldrich, St. Louis, MO, USA) for 1 h with vigorous shaking at 37°C.

Article Title: Decellularized tendon matrix methods and uses thereof
Article Snippet: To make the enzymatic solutions, collagenase I (Life Technologies) was used at 2 mg/l mL PBS, collagenase III (MP Biomedicals) at 1 mg/l mL PBS and pepsin (Sigma) at 1 mg/l mL 0.1 M HCl.

Article Title: CCL20-CCR6 signaling alters the metabolic reprogramming to promote the pathogenic Th17 cell differentiation
Article Snippet: Tissues were 33 digested in RPMI medium containing collagenase D (300 units/mL; MP Biomedicals) and DNase I (0.02 34 mg/mL; Invitrogen) at 37°C for 60 minutes.

Article Title: CCL20-CCR6 signaling alters the metabolic reprogramming to promote the pathogenic Th17 cell differentiation
Article Snippet: Tissues were digested in RPMI medium containing collagenase D (300 units/mL; MP Biomedicals) and DNase I (0.02 mg/mL; Invitrogen) at 37°C for 60 minutes.

Incubation:

Article Title: NK Cells Effectively Mediating Antibody-Mediated Kidney Allograft Rejection Requires a Specific Activation Receptor and Graft Expression of the Ligand
Article Snippet: .. Harvested kidney grafts were weighed, minced and digested by incubation with type II collagenase (catalog 1005021, MP Biomedicals, Santa Ana, CA) and DNase I (catalog 10104159001, MilliporeSigma, Burlington, MA) for 45 minutes at 37°C in RPMI medium. .. The digested graft was pressed with the plunger of a 3cc syringe, and after 40 μm filtration and centrifugation, the cells were counted and aliquots stained with the following fluorochrome-conjugated antibodies: FITC-anti-CD107a (catalog 121606, clone;1D4B, BioLegend, San Diego, CA), PE-anti-CD49b (catalog 108907, clone; DX5, BioLegend), APC-anti-NK1.1 (catalog 501129655, clone; PK136, eBioscience, San Diego, CA), PE-Cy7-anti-CD3e (catalog 561100, clone; 145-2C11, BD Biosciences, San Jose, CA), BV421-anti-CD45 (catalog 563890, clone; 30-F11, BD Horizon, San Jose, CA), BV711-anti-CD314 (catalog 563694, clone cx5, BD Horizon), FITC-anti-CD11b (catalog 553310, lone;M1/70, BD Biosciences), PE-anti-Ly6G (catalog 551461, clone 1A8, BD Biosciences), APC-anti-CD45 (catalog 559864, clone; 30-F11, BD Biosciences), APC-Cy7-anti-Ly6C (catalog 128026, clone HK1.4, BioLegend), BV711-anti-F4/80 (catalog 123137, clone BM8, BioLegend) and DAPI solution (catalog 564907, BD Biosciences).

Article Title: SPTBN2 promotes an immunosuppressive tumor microenvironment and cross-resistance to anti-cancer therapies
Article Snippet: .. The tissue was incubated in a digestion solution containing 2 mg/ml Collagenase II (MP Biomedicals, #100502) and 100 μg/ml DNase I (Roche, #10104159001) for 40 minutes with continuous agitation at 200 RPM at 37°C. ..

Recombinant:

Article Title: In vivo safety prediction of recombinant collagen using in vitro simulated degradation analysis, chronic toxicity and immunological evaluation
Article Snippet: .. Recombinant type III collagen (RC) was provided by Jiangsu Trautec Medical Technology Co., Ltd; China-approved recombinant collagen(A-RC) was purchased from Shanxi Jinbo Biopharmaceutical Ltd. Collagenase I was purchased from MP Biomedicals, Inc. in the USA. .. Cathepsin K and calcium chloride were purchased from Sigma-Aldrich Corporation in the USA.

Cell Culture:

Article Title: Method of differentiating pluripotent or epiblast cells into immature oocytes
Article Snippet: More specifically, for example, in the case of a mouse, an ovarian follicle is cultured in α-MEM containing 5% fetal bovine serum (FCS), 2% polyvinylpyrrolidone (these are all manufactured by Sigma Corporation), 150 μM ascorbic acid, 1× GlutaMAX, 1× penicillin/streptomycin, 100 μM 2-mercaptoethanol, 55 μg/mL sodium pyruvate (these are all manufactured by Nacalai Tesque Inc.), 0.1 IU/mL ovarian follicle stimulating hormone (Follistim (registered trademark), manufactured by MSD K.K.), 15 ng/mL BMP15 (bone morphogenetic protein 15), and 15 ng/mL GDF9 (growth differentiation factor 9) (these are all manufactured by R&D Systems, Inc.), for two days from initiation of culture. .. At Day 2 after initiation of the culture, the medium is exchanged with α-MEM containing the above components except BMP15 and GDF9 and the ovarian follicle is cultured in 0.1% TypeIV collagenase (manufactured by MP Biomedicals). ..

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