Incubation:Article Title: Enhanced in vitro osteoblast differentiation on TiO 2 scaffold coated with alginate hydrogel containing simvastatin
Article Snippet: .. Fixed scaffolds were submitted to heat-induced epitope retrieval by heating to 95°C in 0.05% citraconic anhydride in milliQ water (pH 7.4) for 15 min, incubated with monoclonal mouse anti-human collagen type I antibody (I-8H5, MP Biomedicals, Santa Ana, CA, USA) diluted to 1 μg/mL in 1.25% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) with 0.2% Triton X for 1 h at room temperature, followed by incubation for 30 min at room temperature in Cy3-conjugated donkey anti-mouse IgG (Jackson ImmunoResearch, West Grove, PA, USA) diluted in 2.5% BSA/0.05% Tween-20/PBS at a concentration of 2 mg/mL. .. Cell-seeded whole mount stained scaffolds were counterstained using 4′,6-diamidino-2-phenylindole (DAPI), placed on a coverslip and covered with Dako fluorescent mounting medium (Dako, Glostrup, Denmark).
Article Title: Hydrogel coated scaffold
Article Snippet: .. Fixed scaffolds were submitted to heat induced epitope retrieval by heating to 95° C. in 0.05% citraconic anhydride in milliQ water (pH 7.4) for 15 minutes, Incubated with monoclonal mouse anti-human antibodies (I-8H5, MP Biomedicals, Santa Ana, Calif., USA) diluted to 1 μg/ml in 1.25% bovine serum albumin (BSA) in phosphate buffered saline (PBS) with 0.2% Triton X for 1 hour at room temperature, followed by incubation for 30 minutes at room temperature in Cy3-conjugated donkey anti-mouse IgG (Jackson ImmunoResearch, West Grove, Pa., USA) diluted in 2.5% BSA/0.05% Tween-20/PBS at a concentration of 2 mg/ml. .. Cell-seeded whole mount stained scaffolds were counterstained using DAPI and mounted on a coverslip with Dako fluorescent mounting medium (Dako, Glostrup, Denmark).
Article Title: Hydrogel coated scaffold
Article Snippet: .. Fixed scaffolds were submitted to heat induced epitope retrieval by heating to 95° C. in 0.05% citraconic anhydride in milliQ water (pH 7.4) for 15 minutes, incubated with monoclonal mouse anti-human collagen type I antibody (I-8H5, MP Biomedicals, Santa Ana, Calif., USA) diluted to 1 μg/ml in 1.25% bovine serum albumin (BSA) in phosphate buffered saline (PBS) with 0.2% Triton X for 1 hour at room temperature, followed by incubation for 30 minutes at room temperature in Cy3-conjugated donkey anti-mouse IgG (Jackson ImmunoResearch, West Grove, Pa., USA) diluted in 2.5% BSA/0.05% Tween-20/PBS at a concentration of 2 mg/ml. .. Cell-seeded whole mount stained scaffolds were counterstained using DAPI, placed on a coverslip and covered with Dako fluorescent mounting medium (Dako, Glostrup, Denmark).
Article Title: Enhanced in vitro osteoblast differentiation on TiO2 scaffold coated with alginate hydrogel containing simvastatin.
Article Snippet: .. Fixed scaffolds were submitted to heat-induced epitope retrieval by heating to 95°C in 0.05% citraconic anhydride in milliQ water (pH 7.4) for 15 min, incubated with monoclonal mouse anti-human collagen type I antibody (I-8H5, MP Biomedicals, Santa Ana, CA, USA) diluted to 1 μg/mL in 1.25% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) with 0.2% Triton X for 1 h at room temperature, followed by incubation for 30 min at room temperature in Cy3-conjugated donkey anti-mouse IgG (Jackson ImmunoResearch, West Grove, PA, USA) diluted in 2.5% BSA/0.05% Tween-20/PBS at a concentration of 2 mg/mL. .. Cell-seeded whole mount stained scaffolds were counterstained using 4′,6-diamidino-2-phenylindole (DAPI), placed on a coverslip and covered with Dako fluorescent mounting medium (Dako, Glostrup, Denmark).
Saline:Article Title: Enhanced in vitro osteoblast differentiation on TiO 2 scaffold coated with alginate hydrogel containing simvastatin
Article Snippet: .. Fixed scaffolds were submitted to heat-induced epitope retrieval by heating to 95°C in 0.05% citraconic anhydride in milliQ water (pH 7.4) for 15 min, incubated with monoclonal mouse anti-human collagen type I antibody (I-8H5, MP Biomedicals, Santa Ana, CA, USA) diluted to 1 μg/mL in 1.25% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) with 0.2% Triton X for 1 h at room temperature, followed by incubation for 30 min at room temperature in Cy3-conjugated donkey anti-mouse IgG (Jackson ImmunoResearch, West Grove, PA, USA) diluted in 2.5% BSA/0.05% Tween-20/PBS at a concentration of 2 mg/mL. .. Cell-seeded whole mount stained scaffolds were counterstained using 4′,6-diamidino-2-phenylindole (DAPI), placed on a coverslip and covered with Dako fluorescent mounting medium (Dako, Glostrup, Denmark).
Article Title: Hydrogel coated scaffold
Article Snippet: .. Fixed scaffolds were submitted to heat induced epitope retrieval by heating to 95° C. in 0.05% citraconic anhydride in milliQ water (pH 7.4) for 15 minutes, Incubated with monoclonal mouse anti-human antibodies (I-8H5, MP Biomedicals, Santa Ana, Calif., USA) diluted to 1 μg/ml in 1.25% bovine serum albumin (BSA) in phosphate buffered saline (PBS) with 0.2% Triton X for 1 hour at room temperature, followed by incubation for 30 minutes at room temperature in Cy3-conjugated donkey anti-mouse IgG (Jackson ImmunoResearch, West Grove, Pa., USA) diluted in 2.5% BSA/0.05% Tween-20/PBS at a concentration of 2 mg/ml. .. Cell-seeded whole mount stained scaffolds were counterstained using DAPI and mounted on a coverslip with Dako fluorescent mounting medium (Dako, Glostrup, Denmark).
Article Title: Hydrogel coated scaffold
Article Snippet: .. Fixed scaffolds were submitted to heat induced epitope retrieval by heating to 95° C. in 0.05% citraconic anhydride in milliQ water (pH 7.4) for 15 minutes, incubated with monoclonal mouse anti-human collagen type I antibody (I-8H5, MP Biomedicals, Santa Ana, Calif., USA) diluted to 1 μg/ml in 1.25% bovine serum albumin (BSA) in phosphate buffered saline (PBS) with 0.2% Triton X for 1 hour at room temperature, followed by incubation for 30 minutes at room temperature in Cy3-conjugated donkey anti-mouse IgG (Jackson ImmunoResearch, West Grove, Pa., USA) diluted in 2.5% BSA/0.05% Tween-20/PBS at a concentration of 2 mg/ml. .. Cell-seeded whole mount stained scaffolds were counterstained using DAPI, placed on a coverslip and covered with Dako fluorescent mounting medium (Dako, Glostrup, Denmark).
Article Title: Enhanced in vitro osteoblast differentiation on TiO2 scaffold coated with alginate hydrogel containing simvastatin.
Article Snippet: .. Fixed scaffolds were submitted to heat-induced epitope retrieval by heating to 95°C in 0.05% citraconic anhydride in milliQ water (pH 7.4) for 15 min, incubated with monoclonal mouse anti-human collagen type I antibody (I-8H5, MP Biomedicals, Santa Ana, CA, USA) diluted to 1 μg/mL in 1.25% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) with 0.2% Triton X for 1 h at room temperature, followed by incubation for 30 min at room temperature in Cy3-conjugated donkey anti-mouse IgG (Jackson ImmunoResearch, West Grove, PA, USA) diluted in 2.5% BSA/0.05% Tween-20/PBS at a concentration of 2 mg/mL. .. Cell-seeded whole mount stained scaffolds were counterstained using 4′,6-diamidino-2-phenylindole (DAPI), placed on a coverslip and covered with Dako fluorescent mounting medium (Dako, Glostrup, Denmark).
Concentration Assay:Article Title: Enhanced in vitro osteoblast differentiation on TiO 2 scaffold coated with alginate hydrogel containing simvastatin
Article Snippet: .. Fixed scaffolds were submitted to heat-induced epitope retrieval by heating to 95°C in 0.05% citraconic anhydride in milliQ water (pH 7.4) for 15 min, incubated with monoclonal mouse anti-human collagen type I antibody (I-8H5, MP Biomedicals, Santa Ana, CA, USA) diluted to 1 μg/mL in 1.25% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) with 0.2% Triton X for 1 h at room temperature, followed by incubation for 30 min at room temperature in Cy3-conjugated donkey anti-mouse IgG (Jackson ImmunoResearch, West Grove, PA, USA) diluted in 2.5% BSA/0.05% Tween-20/PBS at a concentration of 2 mg/mL. .. Cell-seeded whole mount stained scaffolds were counterstained using 4′,6-diamidino-2-phenylindole (DAPI), placed on a coverslip and covered with Dako fluorescent mounting medium (Dako, Glostrup, Denmark).
Article Title: Hydrogel coated scaffold
Article Snippet: .. Fixed scaffolds were submitted to heat induced epitope retrieval by heating to 95° C. in 0.05% citraconic anhydride in milliQ water (pH 7.4) for 15 minutes, Incubated with monoclonal mouse anti-human antibodies (I-8H5, MP Biomedicals, Santa Ana, Calif., USA) diluted to 1 μg/ml in 1.25% bovine serum albumin (BSA) in phosphate buffered saline (PBS) with 0.2% Triton X for 1 hour at room temperature, followed by incubation for 30 minutes at room temperature in Cy3-conjugated donkey anti-mouse IgG (Jackson ImmunoResearch, West Grove, Pa., USA) diluted in 2.5% BSA/0.05% Tween-20/PBS at a concentration of 2 mg/ml. .. Cell-seeded whole mount stained scaffolds were counterstained using DAPI and mounted on a coverslip with Dako fluorescent mounting medium (Dako, Glostrup, Denmark).
Article Title: Hydrogel coated scaffold
Article Snippet: .. Fixed scaffolds were submitted to heat induced epitope retrieval by heating to 95° C. in 0.05% citraconic anhydride in milliQ water (pH 7.4) for 15 minutes, incubated with monoclonal mouse anti-human collagen type I antibody (I-8H5, MP Biomedicals, Santa Ana, Calif., USA) diluted to 1 μg/ml in 1.25% bovine serum albumin (BSA) in phosphate buffered saline (PBS) with 0.2% Triton X for 1 hour at room temperature, followed by incubation for 30 minutes at room temperature in Cy3-conjugated donkey anti-mouse IgG (Jackson ImmunoResearch, West Grove, Pa., USA) diluted in 2.5% BSA/0.05% Tween-20/PBS at a concentration of 2 mg/ml. .. Cell-seeded whole mount stained scaffolds were counterstained using DAPI, placed on a coverslip and covered with Dako fluorescent mounting medium (Dako, Glostrup, Denmark).
Article Title: Enhanced in vitro osteoblast differentiation on TiO2 scaffold coated with alginate hydrogel containing simvastatin.
Article Snippet: .. Fixed scaffolds were submitted to heat-induced epitope retrieval by heating to 95°C in 0.05% citraconic anhydride in milliQ water (pH 7.4) for 15 min, incubated with monoclonal mouse anti-human collagen type I antibody (I-8H5, MP Biomedicals, Santa Ana, CA, USA) diluted to 1 μg/mL in 1.25% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) with 0.2% Triton X for 1 h at room temperature, followed by incubation for 30 min at room temperature in Cy3-conjugated donkey anti-mouse IgG (Jackson ImmunoResearch, West Grove, PA, USA) diluted in 2.5% BSA/0.05% Tween-20/PBS at a concentration of 2 mg/mL. .. Cell-seeded whole mount stained scaffolds were counterstained using 4′,6-diamidino-2-phenylindole (DAPI), placed on a coverslip and covered with Dako fluorescent mounting medium (Dako, Glostrup, Denmark).
|
