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amikacin amk  (Valiant Co Ltd)

 
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    Valiant Co Ltd amikacin amk
    Critical concentrations for susceptibility testing used for D29 phage qPCR ( 29 ), the agar proportion method, and MIC range of the Sensititre MYCOTB assay
    Amikacin Amk, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/02150342%2E2/Amikacin/pmc03295106-155-33-40
    Average 93 stars, based on 20 article reviews
    amikacin amk - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Real-Time PCR Using Mycobacteriophage DNA for Rapid Phenotypic Drug Susceptibility Results for Mycobacterium tuberculosis"

    Article Title: Real-Time PCR Using Mycobacteriophage DNA for Rapid Phenotypic Drug Susceptibility Results for Mycobacterium tuberculosis

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.01315-11

    Critical concentrations for susceptibility testing used for D29 phage qPCR ( 29 ), the agar proportion method, and MIC range of the Sensititre MYCOTB assay
    Figure Legend Snippet: Critical concentrations for susceptibility testing used for D29 phage qPCR ( 29 ), the agar proportion method, and MIC range of the Sensititre MYCOTB assay

    Techniques Used: Real-time Polymerase Chain Reaction

    Correlation between D29 phage qPCR results and the standard agar proportion method using the cotreatment method. Thirty three M. tuberculosis isolates were tested by phage qPCR and agar proportion assay for susceptibility to rifampin, streptomycin, amikacin, kanamycin, capreomycin, ofloxacin, moxifloxacin, linezolid, and cycloserine. For the phage qPCR assay, TB isolates were coincubated with drugs or control media and phage for 24 h prior to boiling and qPCR. All phage assays were performed in duplicate, and mean qPCR CT is shown. Cutoff threshold values were determined by ROC analysis. The red − symbols indicate standard assay results that were discrepant with D29 phage qPCR.
    Figure Legend Snippet: Correlation between D29 phage qPCR results and the standard agar proportion method using the cotreatment method. Thirty three M. tuberculosis isolates were tested by phage qPCR and agar proportion assay for susceptibility to rifampin, streptomycin, amikacin, kanamycin, capreomycin, ofloxacin, moxifloxacin, linezolid, and cycloserine. For the phage qPCR assay, TB isolates were coincubated with drugs or control media and phage for 24 h prior to boiling and qPCR. All phage assays were performed in duplicate, and mean qPCR CT is shown. Cutoff threshold values were determined by ROC analysis. The red − symbols indicate standard assay results that were discrepant with D29 phage qPCR.

    Techniques Used:

    Accuracy of D29 phage qPCR compared with the agar proportion method
    Figure Legend Snippet: Accuracy of D29 phage qPCR compared with the agar proportion method

    Techniques Used:

    Related Articles

    other:

    Article Title: Piperine inhibits biofilm formation and efflux activity and dysregulates lipid metabolism in Mycobacterium abscessus
    Article Snippet: Ciprofloxacin (CIP; 98%; Thermo Fisher Scientific), clarithromycin (CLA; > 96%; Alfa Aesar, Haverhill, MA), amikacin (AMI; MP Biomedicals, Solon, OH) and cefoxitin (CEF; TCI America, > 98%) stock solutions were prepared in DMSO, filter-sterilized and stored as aliquots at −20 °C.

    Article Title: Identifying alternative antibiotics that elute from calcium sulfate beads for treatment of orthopedic infections.
    Article Snippet: Orthopaedic Trauma Service, Hospital for Special Surgery, New York, New York, USA Orthopaedic Surgery & Rehabilitation, Loyola University Medical Center, Maywood, Illinois, USA Musculoskeletal Integrity Program, Hospital for Special Surgery, New York, New York, USA Infectious Diseases, Hospital for Special Surgery, New York, New York, USA Adult Reconstruction, Hospital for Special Surgery, New York, New York, USA Orthopaedic Trauma Service, Westchester Medical Center, Valhalla, New York, USA

    Article Title: Biofilm formation and polar lipid biosynthesis in Mycobacterium abscessus are inhibited by naphthylmethylpiperazine
    Article Snippet: The following antibiotics were used in our assays: ciprofloxacin (CIP; 98%; Thermo Fisher Scientific), clarithromycin (CLA; >96%; Alfa Aesar, Haverhill, MA), amikacin (AMI; MP Biomedicals, Solon, OH) and cefoxitin (CEF; TCI America, >98%).



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    Structures of natural aminoglycosides (AGs) and their semisynthetic analogs currently used in the clinic and new <t>amikacin</t> analogs developed in this study. Red colors represent the chemically modified features of the semisynthetic AGs and green colors represent the functional groups formed by enzymatic synthesis in this study.
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    Image Search Results


    Structures of natural aminoglycosides (AGs) and their semisynthetic analogs currently used in the clinic and new amikacin analogs developed in this study. Red colors represent the chemically modified features of the semisynthetic AGs and green colors represent the functional groups formed by enzymatic synthesis in this study.

    Journal: Frontiers in Microbiology

    Article Title: Microbial Enzymatic Synthesis of Amikacin Analogs With Antibacterial Activity Against Multidrug-Resistant Pathogens

    doi: 10.3389/fmicb.2021.725916

    Figure Lengend Snippet: Structures of natural aminoglycosides (AGs) and their semisynthetic analogs currently used in the clinic and new amikacin analogs developed in this study. Red colors represent the chemically modified features of the semisynthetic AGs and green colors represent the functional groups formed by enzymatic synthesis in this study.

    Article Snippet: To produce 3 ″ - N -methyl-amikacin, purified GenN (500 μM) was incubated with 2 mM amikacin (MP Biomedicals, LLC, Parc d’innovation, Illkirch, France) and 2 mM S -adenosyl- L -methionine (SAM) in 50 mM Tris–HCl (pH 7.5) at 30°C for 12 h. Two new 6′- N -acyl-3 ″ - N -methylated amikacin analogs, 6′- N -acetyl-3 ″ - N -methyl-amikacin and 6′- N -propionyl-3 ″ - N -methyl-amikacin, were synthesized using a one-pot enzymatic reaction of AAC(6′)-APH(2 ″ ) and GenN.

    Techniques: Modification, Functional Assay

    Enzymatic synthesis of the amikacin analogs. (A) Structures of the amikacin analogs produced by the microbial enzymatic synthesis. (B) UPLC-qTOF-HR-MS analysis of the amikacin analogs. Chromatograms are shown for selected 3 ″ - N -methyl-amikacin (selected m/z 600.3087), 6′- N -acetyl-3 ″ - N -methyl-amikacin (selected m/z 642.3192), and 6′- N -propionyl-3 ″ - N -methyl-amikacin (selected m/z 656.3349). (C) MS/MS fragmentation patterns of the amikacin analogs.

    Journal: Frontiers in Microbiology

    Article Title: Microbial Enzymatic Synthesis of Amikacin Analogs With Antibacterial Activity Against Multidrug-Resistant Pathogens

    doi: 10.3389/fmicb.2021.725916

    Figure Lengend Snippet: Enzymatic synthesis of the amikacin analogs. (A) Structures of the amikacin analogs produced by the microbial enzymatic synthesis. (B) UPLC-qTOF-HR-MS analysis of the amikacin analogs. Chromatograms are shown for selected 3 ″ - N -methyl-amikacin (selected m/z 600.3087), 6′- N -acetyl-3 ″ - N -methyl-amikacin (selected m/z 642.3192), and 6′- N -propionyl-3 ″ - N -methyl-amikacin (selected m/z 656.3349). (C) MS/MS fragmentation patterns of the amikacin analogs.

    Article Snippet: To produce 3 ″ - N -methyl-amikacin, purified GenN (500 μM) was incubated with 2 mM amikacin (MP Biomedicals, LLC, Parc d’innovation, Illkirch, France) and 2 mM S -adenosyl- L -methionine (SAM) in 50 mM Tris–HCl (pH 7.5) at 30°C for 12 h. Two new 6′- N -acyl-3 ″ - N -methylated amikacin analogs, 6′- N -acetyl-3 ″ - N -methyl-amikacin and 6′- N -propionyl-3 ″ - N -methyl-amikacin, were synthesized using a one-pot enzymatic reaction of AAC(6′)-APH(2 ″ ) and GenN.

    Techniques: Produced, Tandem Mass Spectroscopy

    1 H and 13 C NMR data of novel amikacin analogs in D 2 O.

    Journal: Frontiers in Microbiology

    Article Title: Microbial Enzymatic Synthesis of Amikacin Analogs With Antibacterial Activity Against Multidrug-Resistant Pathogens

    doi: 10.3389/fmicb.2021.725916

    Figure Lengend Snippet: 1 H and 13 C NMR data of novel amikacin analogs in D 2 O.

    Article Snippet: To produce 3 ″ - N -methyl-amikacin, purified GenN (500 μM) was incubated with 2 mM amikacin (MP Biomedicals, LLC, Parc d’innovation, Illkirch, France) and 2 mM S -adenosyl- L -methionine (SAM) in 50 mM Tris–HCl (pH 7.5) at 30°C for 12 h. Two new 6′- N -acyl-3 ″ - N -methylated amikacin analogs, 6′- N -acetyl-3 ″ - N -methyl-amikacin and 6′- N -propionyl-3 ″ - N -methyl-amikacin, were synthesized using a one-pot enzymatic reaction of AAC(6′)-APH(2 ″ ) and GenN.

    Techniques:

    Antibacterial activity of amikacin and its analogs.

    Journal: Frontiers in Microbiology

    Article Title: Microbial Enzymatic Synthesis of Amikacin Analogs With Antibacterial Activity Against Multidrug-Resistant Pathogens

    doi: 10.3389/fmicb.2021.725916

    Figure Lengend Snippet: Antibacterial activity of amikacin and its analogs.

    Article Snippet: To produce 3 ″ - N -methyl-amikacin, purified GenN (500 μM) was incubated with 2 mM amikacin (MP Biomedicals, LLC, Parc d’innovation, Illkirch, France) and 2 mM S -adenosyl- L -methionine (SAM) in 50 mM Tris–HCl (pH 7.5) at 30°C for 12 h. Two new 6′- N -acyl-3 ″ - N -methylated amikacin analogs, 6′- N -acetyl-3 ″ - N -methyl-amikacin and 6′- N -propionyl-3 ″ - N -methyl-amikacin, were synthesized using a one-pot enzymatic reaction of AAC(6′)-APH(2 ″ ) and GenN.

    Techniques: Activity Assay

    Cytotoxicity of newly synthesized amikacin analogs against three different mammalian renal cell lines. LD 50 (μM) of amikacin analogs against (A) HEK-293, (B) LLC-PK1, and (C) A-498 cells. Results with ∗∗ p < 0.01 and ∗ p < 0.05 were considered significant within the treatment groups.

    Journal: Frontiers in Microbiology

    Article Title: Microbial Enzymatic Synthesis of Amikacin Analogs With Antibacterial Activity Against Multidrug-Resistant Pathogens

    doi: 10.3389/fmicb.2021.725916

    Figure Lengend Snippet: Cytotoxicity of newly synthesized amikacin analogs against three different mammalian renal cell lines. LD 50 (μM) of amikacin analogs against (A) HEK-293, (B) LLC-PK1, and (C) A-498 cells. Results with ∗∗ p < 0.01 and ∗ p < 0.05 were considered significant within the treatment groups.

    Article Snippet: To produce 3 ″ - N -methyl-amikacin, purified GenN (500 μM) was incubated with 2 mM amikacin (MP Biomedicals, LLC, Parc d’innovation, Illkirch, France) and 2 mM S -adenosyl- L -methionine (SAM) in 50 mM Tris–HCl (pH 7.5) at 30°C for 12 h. Two new 6′- N -acyl-3 ″ - N -methylated amikacin analogs, 6′- N -acetyl-3 ″ - N -methyl-amikacin and 6′- N -propionyl-3 ″ - N -methyl-amikacin, were synthesized using a one-pot enzymatic reaction of AAC(6′)-APH(2 ″ ) and GenN.

    Techniques: Synthesized