β galactosidase (Valiant Co Ltd)
96
Structured Review
Valiant Co Ltd
β galactosidase
β Galactosidase, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 292 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0215003901/%CE%B2-Galactosidase/pmc04518195-0-0-6
Average 96 stars, based on 292 article reviews
β Galactosidase, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 292 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0215003901/%CE%B2-Galactosidase/pmc04518195-0-0-6
Average 96 stars, based on 292 article reviews
β galactosidase - by Bioz Stars,
2026-09
96/100 stars
Images
1) Product Images from "Inner retinal change in a novel rd1-FTL mouse model of retinal degeneration"
Article Title: Inner retinal change in a novel rd1-FTL mouse model of retinal degeneration
Journal: Frontiers in Cellular Neuroscience
doi: 10.3389/fncel.2015.00293
Figure Legend Snippet: Fos-Tau-LacZ (FTL) reporter expression increases in the rd1-FTL retina as a function of age. Flatmount retinae from control FTL ( A , P135), rd1-FTL ( B , P105; C , P135; D , P240; E , dark adapted rd1-FTL aged P210) mice were processed for FTL expression using β-galactosidase histochemistry. In contrast to the wild-type retina that showed FTL labeling throughout the retina (A) , the rd1-FTL aged P105 (B) showed no FTL labeling. However, at P135 (C) rd1-FTL retina showed distinct labeling near the optic nerve, that subsequently expanded across the central retina (D , E) . The expression of the FTL reporter as a function of retinal area was quantified at different ages from P105 to P365 (F) . Data presented as mean ± SEM, n ≥ 8 per group, ∗∗∗∗ p < 0.0001 (one-way ANOVA). Scale bar for (A – E) = 1 mm.
Techniques Used: Expressing, Labeling
Figure Legend Snippet: Amacrine and ganglion cells express the FTL reporter within the inner retina of rd1-FTL. Retinal sections of rd1-FTL mouse (6 months of age) were labeled with anti-β-galactosidase ( A , C , E ; red), which identifies FTL reporter expression (see white arrows in A , C ). Retinal sections were co-labeled with GABA (B) , or glycine (D) showing FTL was co-localized within subpopulations of GABA immunoreactive ( B , white arrows) and glycine immunoreactive ( D , white arrows) amacrine cells. Retinal wholemounts were double labeled with anti-β-galactosidase and tyrosine hydroxylase ( E , F ; respectively) and no co-localization was evident. Rd1-FTL wholemount from a 4.5 month-old animal was also labeled using β-galactosidase histochemistry showing ganglion cell axons projecting to the optic nerve express the reporter ( G , black arrows). This ganglion cell expression was confirmed in vertical sections of rd1-FTL retina, which were triple labeled for β-galactosidase (red), GABA (green) and NeuN (Blue). There are numerous cells in the GCL that are co-labeled with FTL and NeuN ( H , white arrows). Abbreviations: INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer, OD, optic disk. Scale bar for (A – F , H) : 50 μm; (G) : 250 μm.
Techniques Used: Labeling, Expressing
Figure Legend Snippet: Fos-Tau-LacZ reporter expression in the rd1-FTL retina is not due to cell death nor correlated with remodeled inner retina. Total and inner nuclear layer thickness measures were measured from vertical retinal sections in areas exhibiting FTL expression, as well as neighboring areas showing no FTL expression (A) . Additionally, vertical sections of rd1-FTL retina were labeled for FTL (anti-β-galactosidase, green) and DAPI (blue). Inner retinal layer and total retinal thickness’ were similar between FTL + sand FTL - regions (A) . Increased FTL reporter expression was observed in inner retinal regions where the structure remained intact (B) , as well as in regions where there was evidence of anatomical remodeling (arrows, C ). Furthermore, other regions that exhibited DAPI labeled nuclei which were mislocated within the IPL, were FTL negative (arrows, D ). Data presented as mean ± SEM, n ≥ 6 per group, p > 0.05 (two-way ANOVA). Abbreviations: INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bar: 50 μm.
Techniques Used: Expressing, Labeling
Figure Legend Snippet: Fos-Tau-LacZ positive regions within the rd1-FTL retina show Müller cell alterations. Vertical sections of the rd1-FTL mouse retina (6 month-old) were labeled for FTL ( A , D ; anti-β-galactosidase, red) and glutamine synthetase (GS; B , E ; green) and areas of co-localisation assessed (C , F) . Regions that are intensely labeled for FTL correspond to regions that show low GS labeling (white arrow; B, C ). High magnification of the FTL expressing region within the rd1-FTL retina is indicated by the white bracket and corresponds to an area of low GS labeling. Abbreviations: INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bar: 250 μm (A – C) , 50 μm (D – F) .
Techniques Used: Labeling, Expressing
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