other:Article Title: Programmable Assembly of Mechanically Robust and Functional Polymer–Spore Biocomposites in Organic Solvent
Article Snippet: Yeast extract powder was purchased from MP Biomedicals (CA, USA).
Bicinchoninic Acid Protein Assay:Article Title: Macroscopic Assembly of Materials with Engineered Bacterial Spores via Coiled-Coil Interaction.
Article Snippet: .. Chemical Label Supplier Catalog # Sodium Hydroxide, pellet Macron Fine Chemicals 7708-06 Sodium Chloride Fischer Chemical 5271-10 Dithiothreitol (DTT) Fisher Bioreagents BP172-25 Sodium Dodecyl Sulfate (SDS) Fisher Bioreagents BP166-500 BCA Protein Assay Kit Thermo Scientific 23250 SDS Buffer Bio-Rad 1610772 PVDF Membrane Bio-Rad 1620174 3% (w/v) BSA Sigma Aldrich A9418 Tween-20 Chem-Implex 1512 Monoclonal ANTI-FLAG M2 Antibody produced in mouse Millipore Sigma F1804200UG AlexaFluor 647 Goat anti-mouse IgG (H+L) Invitrogen A21236 Casein Hydrolysate, Enzymatic Digest, Ultrapure Thermo Scientific J12855-P5 Yeast Extract Powder MP Biomedicals 103303 Difco Agar Becton, Dickinson and Company 214010 Potassium Chloride Fischer Chemical P217-3 Sodium Phosphate Dibasic Heptahydrate Fischer Chemical 5471-3 Potassium Phosphate Monobasic Fischer Chemical P285-500 Hydrochloric Acid Fischer Chemical A144C-212 Glycerol Macron Fine Chemicals 5092-16 IPTG IBI Scientific 23D3002 Ni-NTA Agarose QiaGen 30230 Lysozyme from Chicken Egg White Research Products International L3810010.0 Sodium Phosphate Monobasic Monohydrate Fischer Chemical 5369-500 Imidazole (>98%) Tokyo Chemical Industry I0001 Carbenecillin Disodium Salt Bioworld 4031000-3 Spectinomycin Dihydrochloride Goldbio 5-140-25 Chloramphenical Research Products International C6100025.0 Laemmli Sample Buffer Bio-Rad 161-0737 Mini-protean TGX Stainfree Gels Bio-Rad 4568096 Precision Plus Protein All Blue Standards Bio-Rad 1610373 Magnesium Sulfate Anhydrous Research Products International M243001000.0 Ammonium Chloride (≥99.5%) Sigma Aldrich ? .. S3 Calcium Chloride Dihydrate Sigma Aldrich C3306500G L-glutamic acid (99%) Thermo Scientific 156210010 L-tryptophan (≥98%) Sigma Aldrich T0254-25G Manganese Chloride Tetrahydrate Fischer Chemical M87-500 Sodium Sulfate Anhydrous Fisher Bioreagents BP354-500 Ammonium Nitrate (≥99.0%) Sigma Aldrich A9642500G Ferrous Chloride Tetrahydrate Fischer Chemical I90-500 Potassium Hydroxide Fischer Chemical P250-3 Bacto Nutrient Broth Becton, Dickinson and Company 234000 Magnesium Sulfate Heptahydrate Fisher Bioreagents BP213-1 Spectra/Por molecularporous membrane tubing (6-8 kDa) Spectrum Med 132660
Membrane:Article Title: Macroscopic Assembly of Materials with Engineered Bacterial Spores via Coiled-Coil Interaction.
Article Snippet: .. Chemical Label Supplier Catalog # Sodium Hydroxide, pellet Macron Fine Chemicals 7708-06 Sodium Chloride Fischer Chemical 5271-10 Dithiothreitol (DTT) Fisher Bioreagents BP172-25 Sodium Dodecyl Sulfate (SDS) Fisher Bioreagents BP166-500 BCA Protein Assay Kit Thermo Scientific 23250 SDS Buffer Bio-Rad 1610772 PVDF Membrane Bio-Rad 1620174 3% (w/v) BSA Sigma Aldrich A9418 Tween-20 Chem-Implex 1512 Monoclonal ANTI-FLAG M2 Antibody produced in mouse Millipore Sigma F1804200UG AlexaFluor 647 Goat anti-mouse IgG (H+L) Invitrogen A21236 Casein Hydrolysate, Enzymatic Digest, Ultrapure Thermo Scientific J12855-P5 Yeast Extract Powder MP Biomedicals 103303 Difco Agar Becton, Dickinson and Company 214010 Potassium Chloride Fischer Chemical P217-3 Sodium Phosphate Dibasic Heptahydrate Fischer Chemical 5471-3 Potassium Phosphate Monobasic Fischer Chemical P285-500 Hydrochloric Acid Fischer Chemical A144C-212 Glycerol Macron Fine Chemicals 5092-16 IPTG IBI Scientific 23D3002 Ni-NTA Agarose QiaGen 30230 Lysozyme from Chicken Egg White Research Products International L3810010.0 Sodium Phosphate Monobasic Monohydrate Fischer Chemical 5369-500 Imidazole (>98%) Tokyo Chemical Industry I0001 Carbenecillin Disodium Salt Bioworld 4031000-3 Spectinomycin Dihydrochloride Goldbio 5-140-25 Chloramphenical Research Products International C6100025.0 Laemmli Sample Buffer Bio-Rad 161-0737 Mini-protean TGX Stainfree Gels Bio-Rad 4568096 Precision Plus Protein All Blue Standards Bio-Rad 1610373 Magnesium Sulfate Anhydrous Research Products International M243001000.0 Ammonium Chloride (≥99.5%) Sigma Aldrich ? .. S3 Calcium Chloride Dihydrate Sigma Aldrich C3306500G L-glutamic acid (99%) Thermo Scientific 156210010 L-tryptophan (≥98%) Sigma Aldrich T0254-25G Manganese Chloride Tetrahydrate Fischer Chemical M87-500 Sodium Sulfate Anhydrous Fisher Bioreagents BP354-500 Ammonium Nitrate (≥99.0%) Sigma Aldrich A9642500G Ferrous Chloride Tetrahydrate Fischer Chemical I90-500 Potassium Hydroxide Fischer Chemical P250-3 Bacto Nutrient Broth Becton, Dickinson and Company 234000 Magnesium Sulfate Heptahydrate Fisher Bioreagents BP213-1 Spectra/Por molecularporous membrane tubing (6-8 kDa) Spectrum Med 132660
Produced:Article Title: Macroscopic Assembly of Materials with Engineered Bacterial Spores via Coiled-Coil Interaction.
Article Snippet: .. Chemical Label Supplier Catalog # Sodium Hydroxide, pellet Macron Fine Chemicals 7708-06 Sodium Chloride Fischer Chemical 5271-10 Dithiothreitol (DTT) Fisher Bioreagents BP172-25 Sodium Dodecyl Sulfate (SDS) Fisher Bioreagents BP166-500 BCA Protein Assay Kit Thermo Scientific 23250 SDS Buffer Bio-Rad 1610772 PVDF Membrane Bio-Rad 1620174 3% (w/v) BSA Sigma Aldrich A9418 Tween-20 Chem-Implex 1512 Monoclonal ANTI-FLAG M2 Antibody produced in mouse Millipore Sigma F1804200UG AlexaFluor 647 Goat anti-mouse IgG (H+L) Invitrogen A21236 Casein Hydrolysate, Enzymatic Digest, Ultrapure Thermo Scientific J12855-P5 Yeast Extract Powder MP Biomedicals 103303 Difco Agar Becton, Dickinson and Company 214010 Potassium Chloride Fischer Chemical P217-3 Sodium Phosphate Dibasic Heptahydrate Fischer Chemical 5471-3 Potassium Phosphate Monobasic Fischer Chemical P285-500 Hydrochloric Acid Fischer Chemical A144C-212 Glycerol Macron Fine Chemicals 5092-16 IPTG IBI Scientific 23D3002 Ni-NTA Agarose QiaGen 30230 Lysozyme from Chicken Egg White Research Products International L3810010.0 Sodium Phosphate Monobasic Monohydrate Fischer Chemical 5369-500 Imidazole (>98%) Tokyo Chemical Industry I0001 Carbenecillin Disodium Salt Bioworld 4031000-3 Spectinomycin Dihydrochloride Goldbio 5-140-25 Chloramphenical Research Products International C6100025.0 Laemmli Sample Buffer Bio-Rad 161-0737 Mini-protean TGX Stainfree Gels Bio-Rad 4568096 Precision Plus Protein All Blue Standards Bio-Rad 1610373 Magnesium Sulfate Anhydrous Research Products International M243001000.0 Ammonium Chloride (≥99.5%) Sigma Aldrich ? .. S3 Calcium Chloride Dihydrate Sigma Aldrich C3306500G L-glutamic acid (99%) Thermo Scientific 156210010 L-tryptophan (≥98%) Sigma Aldrich T0254-25G Manganese Chloride Tetrahydrate Fischer Chemical M87-500 Sodium Sulfate Anhydrous Fisher Bioreagents BP354-500 Ammonium Nitrate (≥99.0%) Sigma Aldrich A9642500G Ferrous Chloride Tetrahydrate Fischer Chemical I90-500 Potassium Hydroxide Fischer Chemical P250-3 Bacto Nutrient Broth Becton, Dickinson and Company 234000 Magnesium Sulfate Heptahydrate Fisher Bioreagents BP213-1 Spectra/Por molecularporous membrane tubing (6-8 kDa) Spectrum Med 132660
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