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Valiant Co Ltd l cystine
L Cystine, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0210145425/L-Cystine/pmc02493344-101-25-27
Average 93 stars, based on 53 article reviews

l cystine - by Bioz Stars, 2026-09

93/100 stars

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Cell Culture:

Article Title: Hippo pathway controls biopterin metabolism to shield adjacent cells from ferroptosis in lung cancer
Article Snippet: Cells were treated with the following reagents for the indicated time: (1S,3 R)-RSL3 (400 nM, #S8155; Selleck Chemicals, Houston, TX, USA), Erastin (5 μM, #17754; Cayman Chemical), L-Buthionine-(S,R)-Sulfoximine (L-BSO, 10 mM, #14484; Cayman Chemical), Sulfasalazine (SAS, 1 mM, #HY-14655; MedChemExpress), actinomycin D (500 nM, #018-21264; Wako), TNF-α (30 ng/mL, #ab206641; Abcam, Cambridge, UK), cycloheximide (30 μg/mL, #037-20991; Wako), Z-VAD-FMK (50 μM for apoptosis inhibition, 20 μM for necroptosis induction, #S7023; Selleck Chemicals), Necrostatin-1 (50 μM, #S8037; Selleck Chemicals), Ferrostatin-1 (5 μM, #17729; Cayman Chemical, Ann Arbor, MI, USA), (6 R)-5,6,7,8-tetrahydro-L-biopterin (BH4, 40 μM, #81880; Cayman Chemical), 7,8-dihydro-L-biopterin (BH2) (40 μM, #81882; Cayman Chemical), and DAHP (10 mM, #81260; Cayman Chemical). .. For cysteine and cystine starvation (CCS) experiments, cells were cultured in RPMI 1640 medium without L-glutamine, L-cysteine, L-cystine, and L-methionine (#1646454; MP Biomedicals) supplemented with 10% dialyzed FBS (#S-FBS-NL-065; Serana), L-glutamine (2 mM, #073-05391; Wako), and l -methionine (0.1 mM, #13038-72; Nacalai) for 24 h. For serum starvation experiments, cells were seeded at 4 × 10 3 cells (for serum-repleted samples) or 1.6 × 10 4 cells (for serum-depleted samples) per one well of 12-well plates and incubated in 10% FBS for 16 h at 37 °C. ..

Incubation:

Generated:

Article Title: Covariation MS uncovers a protein that controls cysteine catabolism
Article Snippet: Flag-LRRC and Flag-LRRC58 ∆256–291 were cloned by Twist Bioscience into pTwist Lenti CMV BSD. .. Cystine depletion medium was generated from a base of Dulbecco’s MEM with l -glutamine and glucose without cysteine and methionine (US Biologicals, D9813, additional bicarbonate added at 1.5 g l −1 ) or Minimum Essential Medium Eagle without cystine, cysteine, glutamine or methionine (MP Biomedicals 091641454). .. As necessary, L-methionine (Sigma Aldrich M5308), l -glutamine (Sigma Aldrich G7513) and l -cystine dihydrochloride (Sigma Aldrich, C6727) were added to the final concentration used in EMEM (ATCC, 30-2003).

Article Title: Covariation MS uncovers a protein that controls cysteine catabolism.
Article Snippet: Haopeng Xiao1,2,3,4,13 ✉, Martha Ordonez, Emma C. Fink, Taylor A. Covington, Hilina B. Woldemichael, Junyi Chen, Mika Sarkin Jain, Milan H. Rohatgi, Shelley M. Wei, Nils Burger, Muneeb A. Sharif, Julius Jan, Yaoyu Wang, Jonathan J. Petrocelli, Katherine Blackmore, Amanda L. Smythers, Bingsen Zhang, Matthew Gilbert, Hakyung Cheong, Sumeet A. Khetarpal, Arianne Smith, Dina Bogoslavski, Yu Lei, Laura Pontano Vaites, Fiona E. McAllister, Nick Van Bruggen, Katherine A. Donovan, Edward L. Huttlin, Evanna L. Mills, Eric S. Fischer & Edward T. Chouchani1,2,12 ✉

Image Search Results

( A ) Actinomycin D-induced cell death was rescued by the apoptosis inhibitor Z-VAD-FMK. Wild-type (WT) LLC cells were pretreated (or not) with Z-VAD-FMK (50 µM) for 1 h, followed by stimulation with Actinomycin D (500 nM) for 24 h. Dead cells were stained with propidium iodide (PI), and the percentage of the PI-negative live cell population was calculated using the flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. **** (a) P = 0.000000000000065; **** (b) P = 0.000000000000065 (one-way ANOVA test followed by Tukey’s multiple comparison test). ( B ) TCZ-induced cell death was rescued by the necroptosis inhibitor Necrostatin-1. WT LLC cells were pretreated (or not) with Necrostatin-1 for 1 h, followed by stimulation with TCZ [T, TNFα (30 ng/mL); C, cycloheximide (30 µg/mL); Z, Z-VAD-FMK (20 µM)] for 24 h. The percentage of live cell population was calculated using PI and flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. **** (a) P = 0.000000012723845; **** (b) P = 0.000000720360306 (one-way ANOVA test followed by Tukey’s multiple comparison test). ( C ) RSL3-induced cell death was rescued by the ferroptosis inhibitor Ferrostatin-1. WT LLC cells were pretreated (or not) with Ferrostatin-1 (5 µM) for 30 min, followed by stimulation with RSL3 (400 nM) for 10 h. The percentage of live cell population was calculated using PI and flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. **** (a) P = 0.000000048729652; **** (b) P = 0.000000056548852 (one-way ANOVA test followed by Tukey’s multiple comparison test). ( D ) YAP/TAZ loss sensitizes LLC cells to apoptosis. WT and YAP/TAZ dKO LLC cells were treated with Actinomycin D (500 nM) for 24 h, followed by cell viability assay using flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. **** P = 0.000030539048461 (one-way ANOVA test followed by Tukey’s multiple comparison test). ( E ) YAP/TAZ loss sensitizes LLC cells to necroptosis. WT and YAP/TAZ dKO LLC cells were treated with TCZ [T, TNFα (30 ng/mL); C, cycloheximide (30 µg/mL); Z, Z-VAD-FMK (20 µM)] for 24 h, followed by cell viability assay using flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. **** P = 0.000000000000054 (one-way ANOVA test followed by Tukey’s multiple comparison test). ( F ) YAP/TAZ loss confers resistance to ferroptosis in LLC cells. WT and YAP/TAZ dKO LLC cells were treated with RSL3 (400 nM) for 10 h, followed by cell viability assay using flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. **** P = 0.000000000000051 (one-way ANOVA test followed by Tukey’s multiple comparison test). ( G ) Cell viability analysis was performed on WT and YAP/TAZ dKO LLC cells treated with indicated concentrations of RSL3 for 10 h, followed by cell viability assay using flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. ( H ) Cell death induced by ferroptosis-inducing reagents or cysteine and cystine starvation (CCS) was rescued by the ferroptosis inhibitor Ferrostatin-1. WT LLC cells were pretreated (or not) with Ferrostatin-1 (5 µM) for 30 min, followed by stimulation with Erastin (5 µM) for 10 h, L-Buthionine-(S,R)-Sulfoximine (L-BSO; 10 mM) for 24 h, or Sulfasalazine (SAS; 1 mM) for 24 h. For CCS, cells were cultured in the cysteine and cystine-starved medium for 24 h. The percentage of live cell population was calculated using flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. **** (a) P = 0.000000000005959; **** (b) P = 0.000000096903963; **** (c) P = 0.000000191080058; **** (d) P = 0.000000000000065 (one-way ANOVA test followed by Tukey’s multiple comparison test). ( I ) YAP/TAZ loss confers resistance to ferroptosis in LLC cells. WT and YAP/TAZ dKO LLC cells were treated with Erastin (5 µM) for 10 h, L-BSO (10 mM) for 24 h, or SAS (1 mM) for 24 h. For CCS, cells were cultured in the cysteine and cystine-starved medium for 24 h. The percentage of live cell population was calculated using flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. **** (a) P = 0.000000000000769; **** (b) P = 0.000000000883037; **** (c) P = 0.000000000024749; **** (d) P = 0.000000000000051 (one-way ANOVA test followed by Tukey’s multiple comparison test).

Journal: EMBO Reports

Article Title: Hippo pathway controls biopterin metabolism to shield adjacent cells from ferroptosis in lung cancer

doi: 10.1038/s44319-025-00515-4

Figure Lengend Snippet: ( A ) Actinomycin D-induced cell death was rescued by the apoptosis inhibitor Z-VAD-FMK. Wild-type (WT) LLC cells were pretreated (or not) with Z-VAD-FMK (50 µM) for 1 h, followed by stimulation with Actinomycin D (500 nM) for 24 h. Dead cells were stained with propidium iodide (PI), and the percentage of the PI-negative live cell population was calculated using the flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. **** (a) P = 0.000000000000065; **** (b) P = 0.000000000000065 (one-way ANOVA test followed by Tukey’s multiple comparison test). ( B ) TCZ-induced cell death was rescued by the necroptosis inhibitor Necrostatin-1. WT LLC cells were pretreated (or not) with Necrostatin-1 for 1 h, followed by stimulation with TCZ [T, TNFα (30 ng/mL); C, cycloheximide (30 µg/mL); Z, Z-VAD-FMK (20 µM)] for 24 h. The percentage of live cell population was calculated using PI and flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. **** (a) P = 0.000000012723845; **** (b) P = 0.000000720360306 (one-way ANOVA test followed by Tukey’s multiple comparison test). ( C ) RSL3-induced cell death was rescued by the ferroptosis inhibitor Ferrostatin-1. WT LLC cells were pretreated (or not) with Ferrostatin-1 (5 µM) for 30 min, followed by stimulation with RSL3 (400 nM) for 10 h. The percentage of live cell population was calculated using PI and flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. **** (a) P = 0.000000048729652; **** (b) P = 0.000000056548852 (one-way ANOVA test followed by Tukey’s multiple comparison test). ( D ) YAP/TAZ loss sensitizes LLC cells to apoptosis. WT and YAP/TAZ dKO LLC cells were treated with Actinomycin D (500 nM) for 24 h, followed by cell viability assay using flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. **** P = 0.000030539048461 (one-way ANOVA test followed by Tukey’s multiple comparison test). ( E ) YAP/TAZ loss sensitizes LLC cells to necroptosis. WT and YAP/TAZ dKO LLC cells were treated with TCZ [T, TNFα (30 ng/mL); C, cycloheximide (30 µg/mL); Z, Z-VAD-FMK (20 µM)] for 24 h, followed by cell viability assay using flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. **** P = 0.000000000000054 (one-way ANOVA test followed by Tukey’s multiple comparison test). ( F ) YAP/TAZ loss confers resistance to ferroptosis in LLC cells. WT and YAP/TAZ dKO LLC cells were treated with RSL3 (400 nM) for 10 h, followed by cell viability assay using flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. **** P = 0.000000000000051 (one-way ANOVA test followed by Tukey’s multiple comparison test). ( G ) Cell viability analysis was performed on WT and YAP/TAZ dKO LLC cells treated with indicated concentrations of RSL3 for 10 h, followed by cell viability assay using flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. ( H ) Cell death induced by ferroptosis-inducing reagents or cysteine and cystine starvation (CCS) was rescued by the ferroptosis inhibitor Ferrostatin-1. WT LLC cells were pretreated (or not) with Ferrostatin-1 (5 µM) for 30 min, followed by stimulation with Erastin (5 µM) for 10 h, L-Buthionine-(S,R)-Sulfoximine (L-BSO; 10 mM) for 24 h, or Sulfasalazine (SAS; 1 mM) for 24 h. For CCS, cells were cultured in the cysteine and cystine-starved medium for 24 h. The percentage of live cell population was calculated using flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. **** (a) P = 0.000000000005959; **** (b) P = 0.000000096903963; **** (c) P = 0.000000191080058; **** (d) P = 0.000000000000065 (one-way ANOVA test followed by Tukey’s multiple comparison test). ( I ) YAP/TAZ loss confers resistance to ferroptosis in LLC cells. WT and YAP/TAZ dKO LLC cells were treated with Erastin (5 µM) for 10 h, L-BSO (10 mM) for 24 h, or SAS (1 mM) for 24 h. For CCS, cells were cultured in the cysteine and cystine-starved medium for 24 h. The percentage of live cell population was calculated using flow cytometer. Data are means ± SD of three biologically independent samples from a representative experiment. **** (a) P = 0.000000000000769; **** (b) P = 0.000000000883037; **** (c) P = 0.000000000024749; **** (d) P = 0.000000000000051 (one-way ANOVA test followed by Tukey’s multiple comparison test).

Article Snippet: For cysteine and cystine starvation (CCS) experiments, cells were cultured in RPMI 1640 medium without L-glutamine, L-cysteine, L-cystine, and L-methionine (#1646454; MP Biomedicals) supplemented with 10% dialyzed FBS (#S-FBS-NL-065; Serana), L-glutamine (2 mM, #073-05391; Wako), and l -methionine (0.1 mM, #13038-72; Nacalai) for 24 h. For serum starvation experiments, cells were seeded at 4 × 10 3 cells (for serum-repleted samples) or 1.6 × 10 4 cells (for serum-depleted samples) per one well of 12-well plates and incubated in 10% FBS for 16 h at 37 °C.

Techniques: Staining, Flow Cytometry, Comparison, Viability Assay, Cell Culture