cholesteryl hydrogen succinate (Valiant Co Ltd)
Structured Review
Cholesteryl Hydrogen Succinate, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0210137905/Cholesteryl+hydrogen+succinate/pmc06899175-221-14-17
Average 93 stars, based on 9 article reviews
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Labeling:Article Title: Allosteric competition and inhibition in AMPA receptors. Article Snippet: .. Labeling, acquisition and analysis for smFRET HEK293T cells (American Type Culture Collection (ATCC), CRL-3216) overexpressing GluA2FRET or GluA2-γ2FRET receptors were labeled with Nature Structural & Molecular Biology Article https://doi.org/10.1038/s41594-024-01328-0 1:4 ratio of maleimide derivatives of Alexa-555 (donor) and Alexa-647 (acceptor) fluorophores (Invitrogen) in extracellular buffer (135 mM NaCl, 3 mM KCl, 2 mM CaCl2, 20 mM glucose and 20 mM HEPES pH 7.4) at room temperature for 30 min. After labeling, the cells were washed and solubilized for 1 h at 4 °C with buffer containing 1% lauryl maltose neopentyl glycol (Anatrace), 2 mM Article Title: Allosteric competition and inhibition in AMPA receptors Article Snippet: .. HEK293T cells (American Type Culture Collection (ATCC), CRL-3216) overexpressing GluA2 FRET or GluA2-γ2 FRET receptors were labeled with 1:4 ratio of maleimide derivatives of Alexa-555 (donor) and Alexa-647 (acceptor) fluorophores (Invitrogen) in extracellular buffer (135 mM NaCl, 3 mM KCl, 2 mM CaCl 2 , 20 mM glucose and 20 mM HEPES pH 7.4) at room temperature for 30 min. After labeling, the cells were washed and solubilized for 1 h at 4 °C with buffer containing 1% lauryl maltose neopentyl glycol (Anatrace), 2 mM Protease Inhibitor:Article Title: Allosteric competition and inhibition in AMPA receptors. Article Snippet: .. Labeling, acquisition and analysis for smFRET HEK293T cells (American Type Culture Collection (ATCC), CRL-3216) overexpressing GluA2FRET or GluA2-γ2FRET receptors were labeled with Nature Structural & Molecular Biology Article https://doi.org/10.1038/s41594-024-01328-0 1:4 ratio of maleimide derivatives of Alexa-555 (donor) and Alexa-647 (acceptor) fluorophores (Invitrogen) in extracellular buffer (135 mM NaCl, 3 mM KCl, 2 mM CaCl2, 20 mM glucose and 20 mM HEPES pH 7.4) at room temperature for 30 min. After labeling, the cells were washed and solubilized for 1 h at 4 °C with buffer containing 1% lauryl maltose neopentyl glycol (Anatrace), 2 mM Article Title: Allosteric competition and inhibition in AMPA receptors Article Snippet: .. HEK293T cells (American Type Culture Collection (ATCC), CRL-3216) overexpressing GluA2 FRET or GluA2-γ2 FRET receptors were labeled with 1:4 ratio of maleimide derivatives of Alexa-555 (donor) and Alexa-647 (acceptor) fluorophores (Invitrogen) in extracellular buffer (135 mM NaCl, 3 mM KCl, 2 mM CaCl 2 , 20 mM glucose and 20 mM HEPES pH 7.4) at room temperature for 30 min. After labeling, the cells were washed and solubilized for 1 h at 4 °C with buffer containing 1% lauryl maltose neopentyl glycol (Anatrace), 2 mM Article Title: Allosteric Competition and Inhibition in AMPA Receptors Article Snippet: HEK-293 cells overexpressing GluA2 or GluA2-TARPγ2 receptors were labeled with 1:4 ratio of maleimide derivatives of Alexa 555 (donor) and Alexa 647 (acceptor) fluorophores (Invitrogen) in extracellular buffer (135 mM NaCl, 3 mM KCl, 2 mM CaCl2, 20 mM glucose, and 20 mM HEPES, pH 7.4) at room temperature for 30 minutes. .. Post-labeling, the cells were washed and solubilized for 1 h at 4 °C with buffer containing 1% lauryl maltose neopentyl glycol (Anatrace, Maumee, OH, USA), 2 mM Imaging:Article Title: Bi-directional allosteric pathway in NMDA receptor activation and modulation Article Snippet: A physical chamber was constructed on top of the slides using adhesive Hybriwell chambers (Grace Bio-labs, cat. # 43018 C) and press-fit tubing connectors (Grace Bio-labs, cat. # 460003). .. 36 μL of streptavidin solution (0.2 mg/mL Streptavidin (Invitrogen, cat. # S888) in 1X Imaging Buffer (1 mM nDodecyl-beta-D-maltoside (Chem-Impex Int’l Inc., cat. # 21950) and 0.2 mM Article Title: Bi-directional allosteric pathway in NMDA receptor activation and modulation. Article Snippet: A physical chamberwas constructed on topof the slides using adhesiveHybriwell chambers (Grace Bio-labs, cat. # 43018C) and press-fit tubing connectors (Grace Bio-labs, cat. # 460003). .. 36 μL of streptavidin solution (0.2mg/mL Streptavidin (Invitrogen, cat. # S888) in 1X Imaging Buffer (1mMnDodecyl-beta-D-maltoside (Chem-Impex Int’l Inc., cat. # 21950) and 0.2mM Article Title: Bi-directional allosteric pathway in NMDA receptor activation and modulation Article Snippet: A physical chamber was constructed on top of the slides using adhesive Hybriwell chambers (Grace Bio-labs) and press-fit tubing connectors (Fisher Scientific). .. 36 μL of streptavidin solution (0.2 mg/mL Streptavidin (Invitrogen) in 1X Imaging Buffer (1 mM nDodecyl-beta-D-maltoside (Chem-Impex Int’l Inc.) and 0.2 mM Saline:Article Title: Allosteric Competition and Inhibition in AMPA Receptors Article Snippet: HEK-293 cells overexpressing GluA2 or GluA2-TARPγ2 receptors were labeled with 1:4 ratio of maleimide derivatives of Alexa 555 (donor) and Alexa 647 (acceptor) fluorophores (Invitrogen) in extracellular buffer (135 mM NaCl, 3 mM KCl, 2 mM CaCl2, 20 mM glucose, and 20 mM HEPES, pH 7.4) at room temperature for 30 minutes. .. Post-labeling, the cells were washed and solubilized for 1 h at 4 °C with buffer containing 1% lauryl maltose neopentyl glycol (Anatrace, Maumee, OH, USA), 2 mM |
