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Valiant Co Ltd cholesteryl hydrogen succinate
Cholesteryl Hydrogen Succinate, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0210137905/Cholesteryl+hydrogen+succinate/pmc06899175-221-14-17
Average 93 stars, based on 9 article reviews
cholesteryl hydrogen succinate - by Bioz Stars, 2026-09
93/100 stars

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Labeling:

Article Title: Allosteric competition and inhibition in AMPA receptors.
Article Snippet: .. Labeling, acquisition and analysis for smFRET HEK293T cells (American Type Culture Collection (ATCC), CRL-3216) overexpressing GluA2FRET or GluA2-γ2FRET receptors were labeled with Nature Structural & Molecular Biology Article https://doi.org/10.1038/s41594-024-01328-0 1:4 ratio of maleimide derivatives of Alexa-555 (donor) and Alexa-647 (acceptor) fluorophores (Invitrogen) in extracellular buffer (135 mM NaCl, 3 mM KCl, 2 mM CaCl2, 20 mM glucose and 20 mM HEPES pH 7.4) at room temperature for 30 min. After labeling, the cells were washed and solubilized for 1 h at 4 °C with buffer containing 1% lauryl maltose neopentyl glycol (Anatrace), 2 mM cholesteryl hydrogen succinate (CHS; MP Biomedicals) and 1⁄4 protease inhibitor tablet (Pierce) in PBS. ..

Article Title: Allosteric competition and inhibition in AMPA receptors
Article Snippet: .. HEK293T cells (American Type Culture Collection (ATCC), CRL-3216) overexpressing GluA2 FRET or GluA2-γ2 FRET receptors were labeled with 1:4 ratio of maleimide derivatives of Alexa-555 (donor) and Alexa-647 (acceptor) fluorophores (Invitrogen) in extracellular buffer (135 mM NaCl, 3 mM KCl, 2 mM CaCl 2 , 20 mM glucose and 20 mM HEPES pH 7.4) at room temperature for 30 min. After labeling, the cells were washed and solubilized for 1 h at 4 °C with buffer containing 1% lauryl maltose neopentyl glycol (Anatrace), 2 mM cholesteryl hydrogen succinate (CHS; MP Biomedicals) and 1⁄4 protease inhibitor tablet (Pierce) in PBS. ..

Protease Inhibitor:

Article Title: Allosteric competition and inhibition in AMPA receptors.
Article Snippet: .. Labeling, acquisition and analysis for smFRET HEK293T cells (American Type Culture Collection (ATCC), CRL-3216) overexpressing GluA2FRET or GluA2-γ2FRET receptors were labeled with Nature Structural & Molecular Biology Article https://doi.org/10.1038/s41594-024-01328-0 1:4 ratio of maleimide derivatives of Alexa-555 (donor) and Alexa-647 (acceptor) fluorophores (Invitrogen) in extracellular buffer (135 mM NaCl, 3 mM KCl, 2 mM CaCl2, 20 mM glucose and 20 mM HEPES pH 7.4) at room temperature for 30 min. After labeling, the cells were washed and solubilized for 1 h at 4 °C with buffer containing 1% lauryl maltose neopentyl glycol (Anatrace), 2 mM cholesteryl hydrogen succinate (CHS; MP Biomedicals) and 1⁄4 protease inhibitor tablet (Pierce) in PBS. ..

Article Title: Allosteric competition and inhibition in AMPA receptors
Article Snippet: .. HEK293T cells (American Type Culture Collection (ATCC), CRL-3216) overexpressing GluA2 FRET or GluA2-γ2 FRET receptors were labeled with 1:4 ratio of maleimide derivatives of Alexa-555 (donor) and Alexa-647 (acceptor) fluorophores (Invitrogen) in extracellular buffer (135 mM NaCl, 3 mM KCl, 2 mM CaCl 2 , 20 mM glucose and 20 mM HEPES pH 7.4) at room temperature for 30 min. After labeling, the cells were washed and solubilized for 1 h at 4 °C with buffer containing 1% lauryl maltose neopentyl glycol (Anatrace), 2 mM cholesteryl hydrogen succinate (CHS; MP Biomedicals) and 1⁄4 protease inhibitor tablet (Pierce) in PBS. ..

Article Title: Allosteric Competition and Inhibition in AMPA Receptors
Article Snippet: HEK-293 cells overexpressing GluA2 or GluA2-TARPγ2 receptors were labeled with 1:4 ratio of maleimide derivatives of Alexa 555 (donor) and Alexa 647 (acceptor) fluorophores (Invitrogen) in extracellular buffer (135 mM NaCl, 3 mM KCl, 2 mM CaCl2, 20 mM glucose, and 20 mM HEPES, pH 7.4) at room temperature for 30 minutes. .. Post-labeling, the cells were washed and solubilized for 1 h at 4 °C with buffer containing 1% lauryl maltose neopentyl glycol (Anatrace, Maumee, OH, USA), 2 mM cholesteryl hydrogen succinate (MP Biomedicals, Irvine, CA, USA), and 1⁄4 protease inhibitor tablet (PierceTM) in phosphate-buffer saline. ..

Imaging:

Article Title: Bi-directional allosteric pathway in NMDA receptor activation and modulation
Article Snippet: A physical chamber was constructed on top of the slides using adhesive Hybriwell chambers (Grace Bio-labs, cat. # 43018 C) and press-fit tubing connectors (Grace Bio-labs, cat. # 460003). .. 36 μL of streptavidin solution (0.2 mg/mL Streptavidin (Invitrogen, cat. # S888) in 1X Imaging Buffer (1 mM nDodecyl-beta-D-maltoside (Chem-Impex Int’l Inc., cat. # 21950) and 0.2 mM Cholesteryl Hydrogen Succinate (MP Biomedicals, cat. # 1510-21-0) in 1X PBS)) was loaded into the slide chamber and allowed to incubate for 10 min at room temperature. .. When the molecule of interest did not contain a Twin-Strep Tag, 10 nM of biotinylated goat Anti-Mouse IgG (H + L) secondary antibody (Jackson ImmunoResearch Laboratories, Inc., cat. # 115-065-003) was loaded into the chamber and incubated for 20 min.

Article Title: Bi-directional allosteric pathway in NMDA receptor activation and modulation.
Article Snippet: A physical chamberwas constructed on topof the slides using adhesiveHybriwell chambers (Grace Bio-labs, cat. # 43018C) and press-fit tubing connectors (Grace Bio-labs, cat. # 460003). .. 36 μL of streptavidin solution (0.2mg/mL Streptavidin (Invitrogen, cat. # S888) in 1X Imaging Buffer (1mMnDodecyl-beta-D-maltoside (Chem-Impex Int’l Inc., cat. # 21950) and 0.2mM Cholesteryl Hydrogen Succinate (MP Biomedicals, cat. # 1510-21-0) in 1X PBS))was loaded into the slide chamber and allowed to incubate for 10min at room temperature. .. When the molecule of interest did not contain a Twin-Strep Tag, 10 nM of biotinylated goat Anti-Mouse IgG (H + L) secondary antibody (Jackson ImmunoResearch Laboratories, Inc., cat. # 115-065-003)was loaded into the chamber and incubated for 20min.

Article Title: Bi-directional allosteric pathway in NMDA receptor activation and modulation
Article Snippet: A physical chamber was constructed on top of the slides using adhesive Hybriwell chambers (Grace Bio-labs) and press-fit tubing connectors (Fisher Scientific). .. 36 μL of streptavidin solution (0.2 mg/mL Streptavidin (Invitrogen) in 1X Imaging Buffer (1 mM nDodecyl-beta-D-maltoside (Chem-Impex Int’l Inc.) and 0.2 mM Cholesteryl Hydrogen Succinate (MP Biomedicals) in 1X PBS)) was loaded into the slide chamber and allowed to incubate for 10 minutes at room temperature. .. When the molecule of interest did not contain a Twin-Strep Tag, 10 nM of biotinylated goat Anti-Mouse IgG (H+L) secondary antibody (Jackson ImmunoResearch Laboratories, Inc., cat. #115-065-003) was loaded into the chamber and incubated for 20 minutes.

Saline:

Article Title: Allosteric Competition and Inhibition in AMPA Receptors
Article Snippet: HEK-293 cells overexpressing GluA2 or GluA2-TARPγ2 receptors were labeled with 1:4 ratio of maleimide derivatives of Alexa 555 (donor) and Alexa 647 (acceptor) fluorophores (Invitrogen) in extracellular buffer (135 mM NaCl, 3 mM KCl, 2 mM CaCl2, 20 mM glucose, and 20 mM HEPES, pH 7.4) at room temperature for 30 minutes. .. Post-labeling, the cells were washed and solubilized for 1 h at 4 °C with buffer containing 1% lauryl maltose neopentyl glycol (Anatrace, Maumee, OH, USA), 2 mM cholesteryl hydrogen succinate (MP Biomedicals, Irvine, CA, USA), and 1⁄4 protease inhibitor tablet (PierceTM) in phosphate-buffer saline. ..



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Valiant Co Ltd cholesteryl hydrogen succinate
Cholesteryl Hydrogen Succinate, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0210137905/Cholesteryl+hydrogen+succinate/pm39396999-227-24-27
Average 93 stars, based on 1 article reviews
cholesteryl hydrogen succinate - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

93
Valiant Co Ltd cholesteryl hydrogen succinate mp biomedicals
KEY RESOURCES TABLE
Cholesteryl Hydrogen Succinate Mp Biomedicals, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0210137905/Cholesteryl+hydrogen+succinate/pmc08215891-452-72-75
Average 93 stars, based on 1 article reviews
cholesteryl hydrogen succinate mp biomedicals - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

Image Search Results


KEY RESOURCES TABLE

Journal: Methods in enzymology

Article Title: Single molecule FRET methodology for investigating glutamate receptors

doi: 10.1016/bs.mie.2021.02.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals Dulbecco’s Modified Eagle’s Medium (DMEM) GenDepot, Barker, TX Cat# CM001-050 Fetal Bovine Serum (FBS) GenDepot Cat# F0900-050 Penicillin-Streptomycin (P/S) Sigma-Aldrich, St. Louis, MO Cat# P0781 jetPRIME reagent Polyplus, Berkeley, CA Cat# 55-133 Alexa 555-C2- maleimide Invitrogen, Carlsbad, CA Cat# A20346 Alexa 647-C2-maleimide Invitrogen, Carlsbad, CA Cat# A20347 Lauryl Maltose Neopentyl Glycol Anatrace, Maumee, OH Cat# NG310 n-Dodecyl-β-D-maltoside Chem-Impex Int’l Inc., Wood Dale, IL Cat# 21950 Cholesteryl Hydrogen Succinate MP Biomedicals, Solon, OH Cat# 101379 Pierce Protease Inhibitor Mini Tablets ThermoFisher Scientific, Waltham, MA Cat# A32953 Liquinox phosphate-free detergent Fisher Scientific Cat# NC9906065 Vectabond Vector Laboratories, Burlingame, CA Cat# SP-1800 Biotin-terminated Polyethylene glycol (PEG) NOF Corp., Tokyo, Japan Cat# BI-050TS mPEG succinimidyl carbonate Laysan Bio Inc., Arab, AL Cat# MPEG-SC-5000 Sodium bicarbonate Sigma-Aldrich Cat# S6014 MS(PEG)4 Methyl-PEG-NHS-Ester Reagent Thermo Scientific Cat # 22341 Streptavidin Invitrogen Cat# 434301 Glucose Sigma-Aldrich Cat# G8270 Pyranose oxidase Sigma-Aldrich Cat# P4234 Catalase Sigma-Aldrich Cat# C1345 Ascorbic acid Sigma-Aldrich Cat# A7506 Methyl viologen Fisher Scientific Cat# AC227320010 Experimental Models: Cell Lines HEK 293T cells ATCC, Manassas VA Cat# 3216 Recombinant DNA pcDNA3.1 Invitrogen Cat# V790-20 Software and Algorithms Origin Software Origin Lab Matlab MathWorks Open in a separate window KEY RESOURCES TABLE.

Techniques: Modification, Protease Inhibitor, Plasmid Preparation, Recombinant, Software