Homogenization:Article Title: Peptide ligand targeting carbonic anhydrase IX, peptide construct comprising same, and uses thereof
Article Snippet: .. After measuring the weight of each tumor before the experiment, 100 μL of ice-cold homogenization buffer solution (pH 7.4 PBS with traces of DNase 1 in 40 mM EDTA, 6 mg/mL trypsin, 1.6% Triton X-100) was added as buffer solution per 100 mg of organ weight, and homogenization was performed for 5 minutes using FastPrep-24 5G (MPbiomedicals, USA). .. The homogenized organs were measured for fluorescence by dispensing the 100 μL of the homogenate into a 96-well black plate using an EnSpire Multimode Microplate Reader (PerkinElmer, USA) (FIG. 11c).
Incubation:Article Title: A pivotal role for Wnt antagonists in constraining Wnt activity to promote digit joint specification
Article Snippet: Interdigit tissues [between d2-d3, d3-d4, and d4-d5, see ] were dissected with tungsten needles and dissociated with 0.25% Trypsin (Gibco #15090046). .. After 3-4 minutes incubation at 37C followed by gentle trituration, trypsin was inactivated with 1mM AEBSF (MP Biomedicals #193503) followed by addition of 1% serum and isolation of tdTomato labeled interdigit cells by FACS. ..
Gentle:Article Title: A pivotal role for Wnt antagonists in constraining Wnt activity to promote digit joint specification
Article Snippet: Interdigit tissues [between d2-d3, d3-d4, and d4-d5, see ] were dissected with tungsten needles and dissociated with 0.25% Trypsin (Gibco #15090046). .. After 3-4 minutes incubation at 37C followed by gentle trituration, trypsin was inactivated with 1mM AEBSF (MP Biomedicals #193503) followed by addition of 1% serum and isolation of tdTomato labeled interdigit cells by FACS. ..
Isolation:Article Title: A pivotal role for Wnt antagonists in constraining Wnt activity to promote digit joint specification
Article Snippet: Interdigit tissues [between d2-d3, d3-d4, and d4-d5, see ] were dissected with tungsten needles and dissociated with 0.25% Trypsin (Gibco #15090046). .. After 3-4 minutes incubation at 37C followed by gentle trituration, trypsin was inactivated with 1mM AEBSF (MP Biomedicals #193503) followed by addition of 1% serum and isolation of tdTomato labeled interdigit cells by FACS. ..
Labeling:Article Title: A pivotal role for Wnt antagonists in constraining Wnt activity to promote digit joint specification
Article Snippet: Interdigit tissues [between d2-d3, d3-d4, and d4-d5, see ] were dissected with tungsten needles and dissociated with 0.25% Trypsin (Gibco #15090046). .. After 3-4 minutes incubation at 37C followed by gentle trituration, trypsin was inactivated with 1mM AEBSF (MP Biomedicals #193503) followed by addition of 1% serum and isolation of tdTomato labeled interdigit cells by FACS. ..
FACS:Article Title: A pivotal role for Wnt antagonists in constraining Wnt activity to promote digit joint specification
Article Snippet: Interdigit tissues [between d2-d3, d3-d4, and d4-d5, see ] were dissected with tungsten needles and dissociated with 0.25% Trypsin (Gibco #15090046). .. After 3-4 minutes incubation at 37C followed by gentle trituration, trypsin was inactivated with 1mM AEBSF (MP Biomedicals #193503) followed by addition of 1% serum and isolation of tdTomato labeled interdigit cells by FACS. ..
Purification:
Virus:Article Title: Cryo-EM structures of HBV capsids from human cells at near-atomic resolution.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-HBcAg (HBV core antigen) Zeta Corporation Cat #Z2085RL-A Goat anti-Rabbit Ig, human ads-HRP Southern Biotech Cat #4010-05; RRID: AB_2632593 Bacterial and virus strains Stellar TM Escherichia coli HST08 competent cells Takara Bio Cat #636763 E. coli BL21-CodonPlus (DE3) cells New England BioLabs Cat #C2527H Chemicals, peptides, and recombinant proteins DMEM/F12 cell culture medium Gibco Cat #11320-033 Hank’s Balanced Salt Solution (HBSS) (1X) Gibco Cat #14175-095 Penicillin-Streptomycin Solution Cytiva Cat #SV30010 HyClone TM Trypsin 0.25% (1X) Solution Cytiva Cat #SH30042 HyClone TM Fetal Bovine Serum Cytiva Cat #SH30071 YT – Broth Agar (capsules) MP Biomedicals Cat #30112 YT – Broth (powder) MP Biomedicals Cat #3011032 Ampicillin Sigma-Aldrich Cat #A9393 Carbenicillin disodium salt Chem-Impex International Inc. Cat #00049 Sucrose Sigma-Aldrich Cat #S0389 NP-40 core lysis buffer Nguyen & Hu 58 N/A Agarose Sigma-Aldrich Cat #A9539 UltraPure TM 10X TAE Buffer Invitrogen Cat #15558-026 Triton TM X-100 Sigma Life Science Cat #X100 Dialysis buffer This paper and Wang et al. 28 N/A SYPRO® Ruby Protein Gel Stain Lonza Cat #50564 SYBR TM Gold Nucleic Acid Gel Stain (10,000X concentrate in DMSO) Invitrogen Cat #S11494 Critical commercial assays NucleoBond® Xtra Maxi EF DNA purification kit MACHERY-NAGEL Cat #740424 CalPhos TM Mammalian Transfection Kit Takara Bio Cat #631312 Amersham TM ECL TM Western Blotting Detection Reagents Cytiva Cat #RPN2106 Deposited data Cryo-EM structure: HEK-293T Cp183 wildtype T = 4 capsid This paper EMDB: EMD-42823 PDB: 8UYT Cryo-EM structure: HEK-293T Cp183 wildtype T = 3 capsid This paper EMDB: EMD-42822 PDB: 8UYU Cryo-EM structure: HEK-293T Cp183 3A mutant T = 4 capsid This paper EMDB: EMD-42824 PDB: 8UYV Cryo-EM structure: HEK-293T Cp183 3A mutant T = 3 capsid This paper EMDB: EMD-42825 PDB: 8UYW Cryo-EM structure: pre-assembled E. coli Cp149 T = 4 capsid with TX-100 This paper EMDB: EMD-42827 PDB: 8UYY Cryo-EM structure: E. coli Cp149 T = 4 capsid co-assembled with TX-100 This paper EMDB: EMD-42828 PDB: 8UYZ (Continued on next page) Structure 34, 1–14.e1–e5, February 5, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-EM structure: E. coli Cp149 T = 4 capsid (wild-type control) This paper EMDB: EMD-42826 PDB: 8UYX Cryo-EM structure of HBV core [E. coli] Yu et al. 21 EMDB: EMD-2278 PDB: 3J2V Cryo-EM structure: F97L Hepatitis B core protein capsid [E. coli] Bottcher & Nassal 22 EMDB: EMD-0272 PDB: 6HU4 Cryo-EM structure: HBV pgRNA T = 4 NCP icosahedral symmetry [E. coli] Patel et al. 59 EMDB: EMD-11700 PDB: 7ABL X-ray crystallography structure: Human Hepatitis B Virus T = 4 capsid, strain adyw Bourne & Zlotnick 10 PDB: 2G33 X-ray crystallography structure: Human Hepatitis B viral capsid (HBcAg) [E. coli] Wynne et al. 19 PDB: 1QGT Experimental models: cell lines Human embryonic kidney 293T cells Luo et al. 34 N/A Recombinant DNA Plasmid for HEK-293T Cp183 wildtype HBV capsid: pCI-HBc Ludgate et al. 23 N/A Plasmid for HEK-293T Cp183 3A mutant capsid: pCI-HBc-3A Ludgate et al. 23 N/A Plasmid for E. coli Cp149 HBV capsids: pET11a-Cp149 Zlotnick et al. 60 N/A Plasmid for E. coli host RNA-filled Cp183 HBV capsids: pET11c-Cp183 Porterfield et al. 18 N/A Software and algorithms RELION v3.1 Zivanov et al. 61 https://relion.readthedocs.io/en/release-3.
Recombinant:Article Title: Cryo-EM structures of HBV capsids from human cells at near-atomic resolution.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-HBcAg (HBV core antigen) Zeta Corporation Cat #Z2085RL-A Goat anti-Rabbit Ig, human ads-HRP Southern Biotech Cat #4010-05; RRID: AB_2632593 Bacterial and virus strains Stellar TM Escherichia coli HST08 competent cells Takara Bio Cat #636763 E. coli BL21-CodonPlus (DE3) cells New England BioLabs Cat #C2527H Chemicals, peptides, and recombinant proteins DMEM/F12 cell culture medium Gibco Cat #11320-033 Hank’s Balanced Salt Solution (HBSS) (1X) Gibco Cat #14175-095 Penicillin-Streptomycin Solution Cytiva Cat #SV30010 HyClone TM Trypsin 0.25% (1X) Solution Cytiva Cat #SH30042 HyClone TM Fetal Bovine Serum Cytiva Cat #SH30071 YT – Broth Agar (capsules) MP Biomedicals Cat #30112 YT – Broth (powder) MP Biomedicals Cat #3011032 Ampicillin Sigma-Aldrich Cat #A9393 Carbenicillin disodium salt Chem-Impex International Inc. Cat #00049 Sucrose Sigma-Aldrich Cat #S0389 NP-40 core lysis buffer Nguyen & Hu 58 N/A Agarose Sigma-Aldrich Cat #A9539 UltraPure TM 10X TAE Buffer Invitrogen Cat #15558-026 Triton TM X-100 Sigma Life Science Cat #X100 Dialysis buffer This paper and Wang et al. 28 N/A SYPRO® Ruby Protein Gel Stain Lonza Cat #50564 SYBR TM Gold Nucleic Acid Gel Stain (10,000X concentrate in DMSO) Invitrogen Cat #S11494 Critical commercial assays NucleoBond® Xtra Maxi EF DNA purification kit MACHERY-NAGEL Cat #740424 CalPhos TM Mammalian Transfection Kit Takara Bio Cat #631312 Amersham TM ECL TM Western Blotting Detection Reagents Cytiva Cat #RPN2106 Deposited data Cryo-EM structure: HEK-293T Cp183 wildtype T = 4 capsid This paper EMDB: EMD-42823 PDB: 8UYT Cryo-EM structure: HEK-293T Cp183 wildtype T = 3 capsid This paper EMDB: EMD-42822 PDB: 8UYU Cryo-EM structure: HEK-293T Cp183 3A mutant T = 4 capsid This paper EMDB: EMD-42824 PDB: 8UYV Cryo-EM structure: HEK-293T Cp183 3A mutant T = 3 capsid This paper EMDB: EMD-42825 PDB: 8UYW Cryo-EM structure: pre-assembled E. coli Cp149 T = 4 capsid with TX-100 This paper EMDB: EMD-42827 PDB: 8UYY Cryo-EM structure: E. coli Cp149 T = 4 capsid co-assembled with TX-100 This paper EMDB: EMD-42828 PDB: 8UYZ (Continued on next page) Structure 34, 1–14.e1–e5, February 5, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-EM structure: E. coli Cp149 T = 4 capsid (wild-type control) This paper EMDB: EMD-42826 PDB: 8UYX Cryo-EM structure of HBV core [E. coli] Yu et al. 21 EMDB: EMD-2278 PDB: 3J2V Cryo-EM structure: F97L Hepatitis B core protein capsid [E. coli] Bottcher & Nassal 22 EMDB: EMD-0272 PDB: 6HU4 Cryo-EM structure: HBV pgRNA T = 4 NCP icosahedral symmetry [E. coli] Patel et al. 59 EMDB: EMD-11700 PDB: 7ABL X-ray crystallography structure: Human Hepatitis B Virus T = 4 capsid, strain adyw Bourne & Zlotnick 10 PDB: 2G33 X-ray crystallography structure: Human Hepatitis B viral capsid (HBcAg) [E. coli] Wynne et al. 19 PDB: 1QGT Experimental models: cell lines Human embryonic kidney 293T cells Luo et al. 34 N/A Recombinant DNA Plasmid for HEK-293T Cp183 wildtype HBV capsid: pCI-HBc Ludgate et al. 23 N/A Plasmid for HEK-293T Cp183 3A mutant capsid: pCI-HBc-3A Ludgate et al. 23 N/A Plasmid for E. coli Cp149 HBV capsids: pET11a-Cp149 Zlotnick et al. 60 N/A Plasmid for E. coli host RNA-filled Cp183 HBV capsids: pET11c-Cp183 Porterfield et al. 18 N/A Software and algorithms RELION v3.1 Zivanov et al. 61 https://relion.readthedocs.io/en/release-3.
Cell Culture:Article Title: Cryo-EM structures of HBV capsids from human cells at near-atomic resolution.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-HBcAg (HBV core antigen) Zeta Corporation Cat #Z2085RL-A Goat anti-Rabbit Ig, human ads-HRP Southern Biotech Cat #4010-05; RRID: AB_2632593 Bacterial and virus strains Stellar TM Escherichia coli HST08 competent cells Takara Bio Cat #636763 E. coli BL21-CodonPlus (DE3) cells New England BioLabs Cat #C2527H Chemicals, peptides, and recombinant proteins DMEM/F12 cell culture medium Gibco Cat #11320-033 Hank’s Balanced Salt Solution (HBSS) (1X) Gibco Cat #14175-095 Penicillin-Streptomycin Solution Cytiva Cat #SV30010 HyClone TM Trypsin 0.25% (1X) Solution Cytiva Cat #SH30042 HyClone TM Fetal Bovine Serum Cytiva Cat #SH30071 YT – Broth Agar (capsules) MP Biomedicals Cat #30112 YT – Broth (powder) MP Biomedicals Cat #3011032 Ampicillin Sigma-Aldrich Cat #A9393 Carbenicillin disodium salt Chem-Impex International Inc. Cat #00049 Sucrose Sigma-Aldrich Cat #S0389 NP-40 core lysis buffer Nguyen & Hu 58 N/A Agarose Sigma-Aldrich Cat #A9539 UltraPure TM 10X TAE Buffer Invitrogen Cat #15558-026 Triton TM X-100 Sigma Life Science Cat #X100 Dialysis buffer This paper and Wang et al. 28 N/A SYPRO® Ruby Protein Gel Stain Lonza Cat #50564 SYBR TM Gold Nucleic Acid Gel Stain (10,000X concentrate in DMSO) Invitrogen Cat #S11494 Critical commercial assays NucleoBond® Xtra Maxi EF DNA purification kit MACHERY-NAGEL Cat #740424 CalPhos TM Mammalian Transfection Kit Takara Bio Cat #631312 Amersham TM ECL TM Western Blotting Detection Reagents Cytiva Cat #RPN2106 Deposited data Cryo-EM structure: HEK-293T Cp183 wildtype T = 4 capsid This paper EMDB: EMD-42823 PDB: 8UYT Cryo-EM structure: HEK-293T Cp183 wildtype T = 3 capsid This paper EMDB: EMD-42822 PDB: 8UYU Cryo-EM structure: HEK-293T Cp183 3A mutant T = 4 capsid This paper EMDB: EMD-42824 PDB: 8UYV Cryo-EM structure: HEK-293T Cp183 3A mutant T = 3 capsid This paper EMDB: EMD-42825 PDB: 8UYW Cryo-EM structure: pre-assembled E. coli Cp149 T = 4 capsid with TX-100 This paper EMDB: EMD-42827 PDB: 8UYY Cryo-EM structure: E. coli Cp149 T = 4 capsid co-assembled with TX-100 This paper EMDB: EMD-42828 PDB: 8UYZ (Continued on next page) Structure 34, 1–14.e1–e5, February 5, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-EM structure: E. coli Cp149 T = 4 capsid (wild-type control) This paper EMDB: EMD-42826 PDB: 8UYX Cryo-EM structure of HBV core [E. coli] Yu et al. 21 EMDB: EMD-2278 PDB: 3J2V Cryo-EM structure: F97L Hepatitis B core protein capsid [E. coli] Bottcher & Nassal 22 EMDB: EMD-0272 PDB: 6HU4 Cryo-EM structure: HBV pgRNA T = 4 NCP icosahedral symmetry [E. coli] Patel et al. 59 EMDB: EMD-11700 PDB: 7ABL X-ray crystallography structure: Human Hepatitis B Virus T = 4 capsid, strain adyw Bourne & Zlotnick 10 PDB: 2G33 X-ray crystallography structure: Human Hepatitis B viral capsid (HBcAg) [E. coli] Wynne et al. 19 PDB: 1QGT Experimental models: cell lines Human embryonic kidney 293T cells Luo et al. 34 N/A Recombinant DNA Plasmid for HEK-293T Cp183 wildtype HBV capsid: pCI-HBc Ludgate et al. 23 N/A Plasmid for HEK-293T Cp183 3A mutant capsid: pCI-HBc-3A Ludgate et al. 23 N/A Plasmid for E. coli Cp149 HBV capsids: pET11a-Cp149 Zlotnick et al. 60 N/A Plasmid for E. coli host RNA-filled Cp183 HBV capsids: pET11c-Cp183 Porterfield et al. 18 N/A Software and algorithms RELION v3.1 Zivanov et al. 61 https://relion.readthedocs.io/en/release-3.
Capsules:Article Title: Cryo-EM structures of HBV capsids from human cells at near-atomic resolution.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-HBcAg (HBV core antigen) Zeta Corporation Cat #Z2085RL-A Goat anti-Rabbit Ig, human ads-HRP Southern Biotech Cat #4010-05; RRID: AB_2632593 Bacterial and virus strains Stellar TM Escherichia coli HST08 competent cells Takara Bio Cat #636763 E. coli BL21-CodonPlus (DE3) cells New England BioLabs Cat #C2527H Chemicals, peptides, and recombinant proteins DMEM/F12 cell culture medium Gibco Cat #11320-033 Hank’s Balanced Salt Solution (HBSS) (1X) Gibco Cat #14175-095 Penicillin-Streptomycin Solution Cytiva Cat #SV30010 HyClone TM Trypsin 0.25% (1X) Solution Cytiva Cat #SH30042 HyClone TM Fetal Bovine Serum Cytiva Cat #SH30071 YT – Broth Agar (capsules) MP Biomedicals Cat #30112 YT – Broth (powder) MP Biomedicals Cat #3011032 Ampicillin Sigma-Aldrich Cat #A9393 Carbenicillin disodium salt Chem-Impex International Inc. Cat #00049 Sucrose Sigma-Aldrich Cat #S0389 NP-40 core lysis buffer Nguyen & Hu 58 N/A Agarose Sigma-Aldrich Cat #A9539 UltraPure TM 10X TAE Buffer Invitrogen Cat #15558-026 Triton TM X-100 Sigma Life Science Cat #X100 Dialysis buffer This paper and Wang et al. 28 N/A SYPRO® Ruby Protein Gel Stain Lonza Cat #50564 SYBR TM Gold Nucleic Acid Gel Stain (10,000X concentrate in DMSO) Invitrogen Cat #S11494 Critical commercial assays NucleoBond® Xtra Maxi EF DNA purification kit MACHERY-NAGEL Cat #740424 CalPhos TM Mammalian Transfection Kit Takara Bio Cat #631312 Amersham TM ECL TM Western Blotting Detection Reagents Cytiva Cat #RPN2106 Deposited data Cryo-EM structure: HEK-293T Cp183 wildtype T = 4 capsid This paper EMDB: EMD-42823 PDB: 8UYT Cryo-EM structure: HEK-293T Cp183 wildtype T = 3 capsid This paper EMDB: EMD-42822 PDB: 8UYU Cryo-EM structure: HEK-293T Cp183 3A mutant T = 4 capsid This paper EMDB: EMD-42824 PDB: 8UYV Cryo-EM structure: HEK-293T Cp183 3A mutant T = 3 capsid This paper EMDB: EMD-42825 PDB: 8UYW Cryo-EM structure: pre-assembled E. coli Cp149 T = 4 capsid with TX-100 This paper EMDB: EMD-42827 PDB: 8UYY Cryo-EM structure: E. coli Cp149 T = 4 capsid co-assembled with TX-100 This paper EMDB: EMD-42828 PDB: 8UYZ (Continued on next page) Structure 34, 1–14.e1–e5, February 5, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-EM structure: E. coli Cp149 T = 4 capsid (wild-type control) This paper EMDB: EMD-42826 PDB: 8UYX Cryo-EM structure of HBV core [E. coli] Yu et al. 21 EMDB: EMD-2278 PDB: 3J2V Cryo-EM structure: F97L Hepatitis B core protein capsid [E. coli] Bottcher & Nassal 22 EMDB: EMD-0272 PDB: 6HU4 Cryo-EM structure: HBV pgRNA T = 4 NCP icosahedral symmetry [E. coli] Patel et al. 59 EMDB: EMD-11700 PDB: 7ABL X-ray crystallography structure: Human Hepatitis B Virus T = 4 capsid, strain adyw Bourne & Zlotnick 10 PDB: 2G33 X-ray crystallography structure: Human Hepatitis B viral capsid (HBcAg) [E. coli] Wynne et al. 19 PDB: 1QGT Experimental models: cell lines Human embryonic kidney 293T cells Luo et al. 34 N/A Recombinant DNA Plasmid for HEK-293T Cp183 wildtype HBV capsid: pCI-HBc Ludgate et al. 23 N/A Plasmid for HEK-293T Cp183 3A mutant capsid: pCI-HBc-3A Ludgate et al. 23 N/A Plasmid for E. coli Cp149 HBV capsids: pET11a-Cp149 Zlotnick et al. 60 N/A Plasmid for E. coli host RNA-filled Cp183 HBV capsids: pET11c-Cp183 Porterfield et al. 18 N/A Software and algorithms RELION v3.1 Zivanov et al. 61 https://relion.readthedocs.io/en/release-3.
Lysis:Article Title: Cryo-EM structures of HBV capsids from human cells at near-atomic resolution.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-HBcAg (HBV core antigen) Zeta Corporation Cat #Z2085RL-A Goat anti-Rabbit Ig, human ads-HRP Southern Biotech Cat #4010-05; RRID: AB_2632593 Bacterial and virus strains Stellar TM Escherichia coli HST08 competent cells Takara Bio Cat #636763 E. coli BL21-CodonPlus (DE3) cells New England BioLabs Cat #C2527H Chemicals, peptides, and recombinant proteins DMEM/F12 cell culture medium Gibco Cat #11320-033 Hank’s Balanced Salt Solution (HBSS) (1X) Gibco Cat #14175-095 Penicillin-Streptomycin Solution Cytiva Cat #SV30010 HyClone TM Trypsin 0.25% (1X) Solution Cytiva Cat #SH30042 HyClone TM Fetal Bovine Serum Cytiva Cat #SH30071 YT – Broth Agar (capsules) MP Biomedicals Cat #30112 YT – Broth (powder) MP Biomedicals Cat #3011032 Ampicillin Sigma-Aldrich Cat #A9393 Carbenicillin disodium salt Chem-Impex International Inc. Cat #00049 Sucrose Sigma-Aldrich Cat #S0389 NP-40 core lysis buffer Nguyen & Hu 58 N/A Agarose Sigma-Aldrich Cat #A9539 UltraPure TM 10X TAE Buffer Invitrogen Cat #15558-026 Triton TM X-100 Sigma Life Science Cat #X100 Dialysis buffer This paper and Wang et al. 28 N/A SYPRO® Ruby Protein Gel Stain Lonza Cat #50564 SYBR TM Gold Nucleic Acid Gel Stain (10,000X concentrate in DMSO) Invitrogen Cat #S11494 Critical commercial assays NucleoBond® Xtra Maxi EF DNA purification kit MACHERY-NAGEL Cat #740424 CalPhos TM Mammalian Transfection Kit Takara Bio Cat #631312 Amersham TM ECL TM Western Blotting Detection Reagents Cytiva Cat #RPN2106 Deposited data Cryo-EM structure: HEK-293T Cp183 wildtype T = 4 capsid This paper EMDB: EMD-42823 PDB: 8UYT Cryo-EM structure: HEK-293T Cp183 wildtype T = 3 capsid This paper EMDB: EMD-42822 PDB: 8UYU Cryo-EM structure: HEK-293T Cp183 3A mutant T = 4 capsid This paper EMDB: EMD-42824 PDB: 8UYV Cryo-EM structure: HEK-293T Cp183 3A mutant T = 3 capsid This paper EMDB: EMD-42825 PDB: 8UYW Cryo-EM structure: pre-assembled E. coli Cp149 T = 4 capsid with TX-100 This paper EMDB: EMD-42827 PDB: 8UYY Cryo-EM structure: E. coli Cp149 T = 4 capsid co-assembled with TX-100 This paper EMDB: EMD-42828 PDB: 8UYZ (Continued on next page) Structure 34, 1–14.e1–e5, February 5, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-EM structure: E. coli Cp149 T = 4 capsid (wild-type control) This paper EMDB: EMD-42826 PDB: 8UYX Cryo-EM structure of HBV core [E. coli] Yu et al. 21 EMDB: EMD-2278 PDB: 3J2V Cryo-EM structure: F97L Hepatitis B core protein capsid [E. coli] Bottcher & Nassal 22 EMDB: EMD-0272 PDB: 6HU4 Cryo-EM structure: HBV pgRNA T = 4 NCP icosahedral symmetry [E. coli] Patel et al. 59 EMDB: EMD-11700 PDB: 7ABL X-ray crystallography structure: Human Hepatitis B Virus T = 4 capsid, strain adyw Bourne & Zlotnick 10 PDB: 2G33 X-ray crystallography structure: Human Hepatitis B viral capsid (HBcAg) [E. coli] Wynne et al. 19 PDB: 1QGT Experimental models: cell lines Human embryonic kidney 293T cells Luo et al. 34 N/A Recombinant DNA Plasmid for HEK-293T Cp183 wildtype HBV capsid: pCI-HBc Ludgate et al. 23 N/A Plasmid for HEK-293T Cp183 3A mutant capsid: pCI-HBc-3A Ludgate et al. 23 N/A Plasmid for E. coli Cp149 HBV capsids: pET11a-Cp149 Zlotnick et al. 60 N/A Plasmid for E. coli host RNA-filled Cp183 HBV capsids: pET11c-Cp183 Porterfield et al. 18 N/A Software and algorithms RELION v3.1 Zivanov et al. 61 https://relion.readthedocs.io/en/release-3.
Staining:Article Title: Cryo-EM structures of HBV capsids from human cells at near-atomic resolution.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-HBcAg (HBV core antigen) Zeta Corporation Cat #Z2085RL-A Goat anti-Rabbit Ig, human ads-HRP Southern Biotech Cat #4010-05; RRID: AB_2632593 Bacterial and virus strains Stellar TM Escherichia coli HST08 competent cells Takara Bio Cat #636763 E. coli BL21-CodonPlus (DE3) cells New England BioLabs Cat #C2527H Chemicals, peptides, and recombinant proteins DMEM/F12 cell culture medium Gibco Cat #11320-033 Hank’s Balanced Salt Solution (HBSS) (1X) Gibco Cat #14175-095 Penicillin-Streptomycin Solution Cytiva Cat #SV30010 HyClone TM Trypsin 0.25% (1X) Solution Cytiva Cat #SH30042 HyClone TM Fetal Bovine Serum Cytiva Cat #SH30071 YT – Broth Agar (capsules) MP Biomedicals Cat #30112 YT – Broth (powder) MP Biomedicals Cat #3011032 Ampicillin Sigma-Aldrich Cat #A9393 Carbenicillin disodium salt Chem-Impex International Inc. Cat #00049 Sucrose Sigma-Aldrich Cat #S0389 NP-40 core lysis buffer Nguyen & Hu 58 N/A Agarose Sigma-Aldrich Cat #A9539 UltraPure TM 10X TAE Buffer Invitrogen Cat #15558-026 Triton TM X-100 Sigma Life Science Cat #X100 Dialysis buffer This paper and Wang et al. 28 N/A SYPRO® Ruby Protein Gel Stain Lonza Cat #50564 SYBR TM Gold Nucleic Acid Gel Stain (10,000X concentrate in DMSO) Invitrogen Cat #S11494 Critical commercial assays NucleoBond® Xtra Maxi EF DNA purification kit MACHERY-NAGEL Cat #740424 CalPhos TM Mammalian Transfection Kit Takara Bio Cat #631312 Amersham TM ECL TM Western Blotting Detection Reagents Cytiva Cat #RPN2106 Deposited data Cryo-EM structure: HEK-293T Cp183 wildtype T = 4 capsid This paper EMDB: EMD-42823 PDB: 8UYT Cryo-EM structure: HEK-293T Cp183 wildtype T = 3 capsid This paper EMDB: EMD-42822 PDB: 8UYU Cryo-EM structure: HEK-293T Cp183 3A mutant T = 4 capsid This paper EMDB: EMD-42824 PDB: 8UYV Cryo-EM structure: HEK-293T Cp183 3A mutant T = 3 capsid This paper EMDB: EMD-42825 PDB: 8UYW Cryo-EM structure: pre-assembled E. coli Cp149 T = 4 capsid with TX-100 This paper EMDB: EMD-42827 PDB: 8UYY Cryo-EM structure: E. coli Cp149 T = 4 capsid co-assembled with TX-100 This paper EMDB: EMD-42828 PDB: 8UYZ (Continued on next page) Structure 34, 1–14.e1–e5, February 5, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-EM structure: E. coli Cp149 T = 4 capsid (wild-type control) This paper EMDB: EMD-42826 PDB: 8UYX Cryo-EM structure of HBV core [E. coli] Yu et al. 21 EMDB: EMD-2278 PDB: 3J2V Cryo-EM structure: F97L Hepatitis B core protein capsid [E. coli] Bottcher & Nassal 22 EMDB: EMD-0272 PDB: 6HU4 Cryo-EM structure: HBV pgRNA T = 4 NCP icosahedral symmetry [E. coli] Patel et al. 59 EMDB: EMD-11700 PDB: 7ABL X-ray crystallography structure: Human Hepatitis B Virus T = 4 capsid, strain adyw Bourne & Zlotnick 10 PDB: 2G33 X-ray crystallography structure: Human Hepatitis B viral capsid (HBcAg) [E. coli] Wynne et al. 19 PDB: 1QGT Experimental models: cell lines Human embryonic kidney 293T cells Luo et al. 34 N/A Recombinant DNA Plasmid for HEK-293T Cp183 wildtype HBV capsid: pCI-HBc Ludgate et al. 23 N/A Plasmid for HEK-293T Cp183 3A mutant capsid: pCI-HBc-3A Ludgate et al. 23 N/A Plasmid for E. coli Cp149 HBV capsids: pET11a-Cp149 Zlotnick et al. 60 N/A Plasmid for E. coli host RNA-filled Cp183 HBV capsids: pET11c-Cp183 Porterfield et al. 18 N/A Software and algorithms RELION v3.1 Zivanov et al. 61 https://relion.readthedocs.io/en/release-3.
DNA Purification:Article Title: Cryo-EM structures of HBV capsids from human cells at near-atomic resolution.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-HBcAg (HBV core antigen) Zeta Corporation Cat #Z2085RL-A Goat anti-Rabbit Ig, human ads-HRP Southern Biotech Cat #4010-05; RRID: AB_2632593 Bacterial and virus strains Stellar TM Escherichia coli HST08 competent cells Takara Bio Cat #636763 E. coli BL21-CodonPlus (DE3) cells New England BioLabs Cat #C2527H Chemicals, peptides, and recombinant proteins DMEM/F12 cell culture medium Gibco Cat #11320-033 Hank’s Balanced Salt Solution (HBSS) (1X) Gibco Cat #14175-095 Penicillin-Streptomycin Solution Cytiva Cat #SV30010 HyClone TM Trypsin 0.25% (1X) Solution Cytiva Cat #SH30042 HyClone TM Fetal Bovine Serum Cytiva Cat #SH30071 YT – Broth Agar (capsules) MP Biomedicals Cat #30112 YT – Broth (powder) MP Biomedicals Cat #3011032 Ampicillin Sigma-Aldrich Cat #A9393 Carbenicillin disodium salt Chem-Impex International Inc. Cat #00049 Sucrose Sigma-Aldrich Cat #S0389 NP-40 core lysis buffer Nguyen & Hu 58 N/A Agarose Sigma-Aldrich Cat #A9539 UltraPure TM 10X TAE Buffer Invitrogen Cat #15558-026 Triton TM X-100 Sigma Life Science Cat #X100 Dialysis buffer This paper and Wang et al. 28 N/A SYPRO® Ruby Protein Gel Stain Lonza Cat #50564 SYBR TM Gold Nucleic Acid Gel Stain (10,000X concentrate in DMSO) Invitrogen Cat #S11494 Critical commercial assays NucleoBond® Xtra Maxi EF DNA purification kit MACHERY-NAGEL Cat #740424 CalPhos TM Mammalian Transfection Kit Takara Bio Cat #631312 Amersham TM ECL TM Western Blotting Detection Reagents Cytiva Cat #RPN2106 Deposited data Cryo-EM structure: HEK-293T Cp183 wildtype T = 4 capsid This paper EMDB: EMD-42823 PDB: 8UYT Cryo-EM structure: HEK-293T Cp183 wildtype T = 3 capsid This paper EMDB: EMD-42822 PDB: 8UYU Cryo-EM structure: HEK-293T Cp183 3A mutant T = 4 capsid This paper EMDB: EMD-42824 PDB: 8UYV Cryo-EM structure: HEK-293T Cp183 3A mutant T = 3 capsid This paper EMDB: EMD-42825 PDB: 8UYW Cryo-EM structure: pre-assembled E. coli Cp149 T = 4 capsid with TX-100 This paper EMDB: EMD-42827 PDB: 8UYY Cryo-EM structure: E. coli Cp149 T = 4 capsid co-assembled with TX-100 This paper EMDB: EMD-42828 PDB: 8UYZ (Continued on next page) Structure 34, 1–14.e1–e5, February 5, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-EM structure: E. coli Cp149 T = 4 capsid (wild-type control) This paper EMDB: EMD-42826 PDB: 8UYX Cryo-EM structure of HBV core [E. coli] Yu et al. 21 EMDB: EMD-2278 PDB: 3J2V Cryo-EM structure: F97L Hepatitis B core protein capsid [E. coli] Bottcher & Nassal 22 EMDB: EMD-0272 PDB: 6HU4 Cryo-EM structure: HBV pgRNA T = 4 NCP icosahedral symmetry [E. coli] Patel et al. 59 EMDB: EMD-11700 PDB: 7ABL X-ray crystallography structure: Human Hepatitis B Virus T = 4 capsid, strain adyw Bourne & Zlotnick 10 PDB: 2G33 X-ray crystallography structure: Human Hepatitis B viral capsid (HBcAg) [E. coli] Wynne et al. 19 PDB: 1QGT Experimental models: cell lines Human embryonic kidney 293T cells Luo et al. 34 N/A Recombinant DNA Plasmid for HEK-293T Cp183 wildtype HBV capsid: pCI-HBc Ludgate et al. 23 N/A Plasmid for HEK-293T Cp183 3A mutant capsid: pCI-HBc-3A Ludgate et al. 23 N/A Plasmid for E. coli Cp149 HBV capsids: pET11a-Cp149 Zlotnick et al. 60 N/A Plasmid for E. coli host RNA-filled Cp183 HBV capsids: pET11c-Cp183 Porterfield et al. 18 N/A Software and algorithms RELION v3.1 Zivanov et al. 61 https://relion.readthedocs.io/en/release-3.
Transfection:Article Title: Cryo-EM structures of HBV capsids from human cells at near-atomic resolution.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-HBcAg (HBV core antigen) Zeta Corporation Cat #Z2085RL-A Goat anti-Rabbit Ig, human ads-HRP Southern Biotech Cat #4010-05; RRID: AB_2632593 Bacterial and virus strains Stellar TM Escherichia coli HST08 competent cells Takara Bio Cat #636763 E. coli BL21-CodonPlus (DE3) cells New England BioLabs Cat #C2527H Chemicals, peptides, and recombinant proteins DMEM/F12 cell culture medium Gibco Cat #11320-033 Hank’s Balanced Salt Solution (HBSS) (1X) Gibco Cat #14175-095 Penicillin-Streptomycin Solution Cytiva Cat #SV30010 HyClone TM Trypsin 0.25% (1X) Solution Cytiva Cat #SH30042 HyClone TM Fetal Bovine Serum Cytiva Cat #SH30071 YT – Broth Agar (capsules) MP Biomedicals Cat #30112 YT – Broth (powder) MP Biomedicals Cat #3011032 Ampicillin Sigma-Aldrich Cat #A9393 Carbenicillin disodium salt Chem-Impex International Inc. Cat #00049 Sucrose Sigma-Aldrich Cat #S0389 NP-40 core lysis buffer Nguyen & Hu 58 N/A Agarose Sigma-Aldrich Cat #A9539 UltraPure TM 10X TAE Buffer Invitrogen Cat #15558-026 Triton TM X-100 Sigma Life Science Cat #X100 Dialysis buffer This paper and Wang et al. 28 N/A SYPRO® Ruby Protein Gel Stain Lonza Cat #50564 SYBR TM Gold Nucleic Acid Gel Stain (10,000X concentrate in DMSO) Invitrogen Cat #S11494 Critical commercial assays NucleoBond® Xtra Maxi EF DNA purification kit MACHERY-NAGEL Cat #740424 CalPhos TM Mammalian Transfection Kit Takara Bio Cat #631312 Amersham TM ECL TM Western Blotting Detection Reagents Cytiva Cat #RPN2106 Deposited data Cryo-EM structure: HEK-293T Cp183 wildtype T = 4 capsid This paper EMDB: EMD-42823 PDB: 8UYT Cryo-EM structure: HEK-293T Cp183 wildtype T = 3 capsid This paper EMDB: EMD-42822 PDB: 8UYU Cryo-EM structure: HEK-293T Cp183 3A mutant T = 4 capsid This paper EMDB: EMD-42824 PDB: 8UYV Cryo-EM structure: HEK-293T Cp183 3A mutant T = 3 capsid This paper EMDB: EMD-42825 PDB: 8UYW Cryo-EM structure: pre-assembled E. coli Cp149 T = 4 capsid with TX-100 This paper EMDB: EMD-42827 PDB: 8UYY Cryo-EM structure: E. coli Cp149 T = 4 capsid co-assembled with TX-100 This paper EMDB: EMD-42828 PDB: 8UYZ (Continued on next page) Structure 34, 1–14.e1–e5, February 5, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-EM structure: E. coli Cp149 T = 4 capsid (wild-type control) This paper EMDB: EMD-42826 PDB: 8UYX Cryo-EM structure of HBV core [E. coli] Yu et al. 21 EMDB: EMD-2278 PDB: 3J2V Cryo-EM structure: F97L Hepatitis B core protein capsid [E. coli] Bottcher & Nassal 22 EMDB: EMD-0272 PDB: 6HU4 Cryo-EM structure: HBV pgRNA T = 4 NCP icosahedral symmetry [E. coli] Patel et al. 59 EMDB: EMD-11700 PDB: 7ABL X-ray crystallography structure: Human Hepatitis B Virus T = 4 capsid, strain adyw Bourne & Zlotnick 10 PDB: 2G33 X-ray crystallography structure: Human Hepatitis B viral capsid (HBcAg) [E. coli] Wynne et al. 19 PDB: 1QGT Experimental models: cell lines Human embryonic kidney 293T cells Luo et al. 34 N/A Recombinant DNA Plasmid for HEK-293T Cp183 wildtype HBV capsid: pCI-HBc Ludgate et al. 23 N/A Plasmid for HEK-293T Cp183 3A mutant capsid: pCI-HBc-3A Ludgate et al. 23 N/A Plasmid for E. coli Cp149 HBV capsids: pET11a-Cp149 Zlotnick et al. 60 N/A Plasmid for E. coli host RNA-filled Cp183 HBV capsids: pET11c-Cp183 Porterfield et al. 18 N/A Software and algorithms RELION v3.1 Zivanov et al. 61 https://relion.readthedocs.io/en/release-3.
Western Blot:Article Title: Cryo-EM structures of HBV capsids from human cells at near-atomic resolution.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-HBcAg (HBV core antigen) Zeta Corporation Cat #Z2085RL-A Goat anti-Rabbit Ig, human ads-HRP Southern Biotech Cat #4010-05; RRID: AB_2632593 Bacterial and virus strains Stellar TM Escherichia coli HST08 competent cells Takara Bio Cat #636763 E. coli BL21-CodonPlus (DE3) cells New England BioLabs Cat #C2527H Chemicals, peptides, and recombinant proteins DMEM/F12 cell culture medium Gibco Cat #11320-033 Hank’s Balanced Salt Solution (HBSS) (1X) Gibco Cat #14175-095 Penicillin-Streptomycin Solution Cytiva Cat #SV30010 HyClone TM Trypsin 0.25% (1X) Solution Cytiva Cat #SH30042 HyClone TM Fetal Bovine Serum Cytiva Cat #SH30071 YT – Broth Agar (capsules) MP Biomedicals Cat #30112 YT – Broth (powder) MP Biomedicals Cat #3011032 Ampicillin Sigma-Aldrich Cat #A9393 Carbenicillin disodium salt Chem-Impex International Inc. Cat #00049 Sucrose Sigma-Aldrich Cat #S0389 NP-40 core lysis buffer Nguyen & Hu 58 N/A Agarose Sigma-Aldrich Cat #A9539 UltraPure TM 10X TAE Buffer Invitrogen Cat #15558-026 Triton TM X-100 Sigma Life Science Cat #X100 Dialysis buffer This paper and Wang et al. 28 N/A SYPRO® Ruby Protein Gel Stain Lonza Cat #50564 SYBR TM Gold Nucleic Acid Gel Stain (10,000X concentrate in DMSO) Invitrogen Cat #S11494 Critical commercial assays NucleoBond® Xtra Maxi EF DNA purification kit MACHERY-NAGEL Cat #740424 CalPhos TM Mammalian Transfection Kit Takara Bio Cat #631312 Amersham TM ECL TM Western Blotting Detection Reagents Cytiva Cat #RPN2106 Deposited data Cryo-EM structure: HEK-293T Cp183 wildtype T = 4 capsid This paper EMDB: EMD-42823 PDB: 8UYT Cryo-EM structure: HEK-293T Cp183 wildtype T = 3 capsid This paper EMDB: EMD-42822 PDB: 8UYU Cryo-EM structure: HEK-293T Cp183 3A mutant T = 4 capsid This paper EMDB: EMD-42824 PDB: 8UYV Cryo-EM structure: HEK-293T Cp183 3A mutant T = 3 capsid This paper EMDB: EMD-42825 PDB: 8UYW Cryo-EM structure: pre-assembled E. coli Cp149 T = 4 capsid with TX-100 This paper EMDB: EMD-42827 PDB: 8UYY Cryo-EM structure: E. coli Cp149 T = 4 capsid co-assembled with TX-100 This paper EMDB: EMD-42828 PDB: 8UYZ (Continued on next page) Structure 34, 1–14.e1–e5, February 5, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-EM structure: E. coli Cp149 T = 4 capsid (wild-type control) This paper EMDB: EMD-42826 PDB: 8UYX Cryo-EM structure of HBV core [E. coli] Yu et al. 21 EMDB: EMD-2278 PDB: 3J2V Cryo-EM structure: F97L Hepatitis B core protein capsid [E. coli] Bottcher & Nassal 22 EMDB: EMD-0272 PDB: 6HU4 Cryo-EM structure: HBV pgRNA T = 4 NCP icosahedral symmetry [E. coli] Patel et al. 59 EMDB: EMD-11700 PDB: 7ABL X-ray crystallography structure: Human Hepatitis B Virus T = 4 capsid, strain adyw Bourne & Zlotnick 10 PDB: 2G33 X-ray crystallography structure: Human Hepatitis B viral capsid (HBcAg) [E. coli] Wynne et al. 19 PDB: 1QGT Experimental models: cell lines Human embryonic kidney 293T cells Luo et al. 34 N/A Recombinant DNA Plasmid for HEK-293T Cp183 wildtype HBV capsid: pCI-HBc Ludgate et al. 23 N/A Plasmid for HEK-293T Cp183 3A mutant capsid: pCI-HBc-3A Ludgate et al. 23 N/A Plasmid for E. coli Cp149 HBV capsids: pET11a-Cp149 Zlotnick et al. 60 N/A Plasmid for E. coli host RNA-filled Cp183 HBV capsids: pET11c-Cp183 Porterfield et al. 18 N/A Software and algorithms RELION v3.1 Zivanov et al. 61 https://relion.readthedocs.io/en/release-3.
Cryo-EM Sample Prep:Article Title: Cryo-EM structures of HBV capsids from human cells at near-atomic resolution.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-HBcAg (HBV core antigen) Zeta Corporation Cat #Z2085RL-A Goat anti-Rabbit Ig, human ads-HRP Southern Biotech Cat #4010-05; RRID: AB_2632593 Bacterial and virus strains Stellar TM Escherichia coli HST08 competent cells Takara Bio Cat #636763 E. coli BL21-CodonPlus (DE3) cells New England BioLabs Cat #C2527H Chemicals, peptides, and recombinant proteins DMEM/F12 cell culture medium Gibco Cat #11320-033 Hank’s Balanced Salt Solution (HBSS) (1X) Gibco Cat #14175-095 Penicillin-Streptomycin Solution Cytiva Cat #SV30010 HyClone TM Trypsin 0.25% (1X) Solution Cytiva Cat #SH30042 HyClone TM Fetal Bovine Serum Cytiva Cat #SH30071 YT – Broth Agar (capsules) MP Biomedicals Cat #30112 YT – Broth (powder) MP Biomedicals Cat #3011032 Ampicillin Sigma-Aldrich Cat #A9393 Carbenicillin disodium salt Chem-Impex International Inc. Cat #00049 Sucrose Sigma-Aldrich Cat #S0389 NP-40 core lysis buffer Nguyen & Hu 58 N/A Agarose Sigma-Aldrich Cat #A9539 UltraPure TM 10X TAE Buffer Invitrogen Cat #15558-026 Triton TM X-100 Sigma Life Science Cat #X100 Dialysis buffer This paper and Wang et al. 28 N/A SYPRO® Ruby Protein Gel Stain Lonza Cat #50564 SYBR TM Gold Nucleic Acid Gel Stain (10,000X concentrate in DMSO) Invitrogen Cat #S11494 Critical commercial assays NucleoBond® Xtra Maxi EF DNA purification kit MACHERY-NAGEL Cat #740424 CalPhos TM Mammalian Transfection Kit Takara Bio Cat #631312 Amersham TM ECL TM Western Blotting Detection Reagents Cytiva Cat #RPN2106 Deposited data Cryo-EM structure: HEK-293T Cp183 wildtype T = 4 capsid This paper EMDB: EMD-42823 PDB: 8UYT Cryo-EM structure: HEK-293T Cp183 wildtype T = 3 capsid This paper EMDB: EMD-42822 PDB: 8UYU Cryo-EM structure: HEK-293T Cp183 3A mutant T = 4 capsid This paper EMDB: EMD-42824 PDB: 8UYV Cryo-EM structure: HEK-293T Cp183 3A mutant T = 3 capsid This paper EMDB: EMD-42825 PDB: 8UYW Cryo-EM structure: pre-assembled E. coli Cp149 T = 4 capsid with TX-100 This paper EMDB: EMD-42827 PDB: 8UYY Cryo-EM structure: E. coli Cp149 T = 4 capsid co-assembled with TX-100 This paper EMDB: EMD-42828 PDB: 8UYZ (Continued on next page) Structure 34, 1–14.e1–e5, February 5, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-EM structure: E. coli Cp149 T = 4 capsid (wild-type control) This paper EMDB: EMD-42826 PDB: 8UYX Cryo-EM structure of HBV core [E. coli] Yu et al. 21 EMDB: EMD-2278 PDB: 3J2V Cryo-EM structure: F97L Hepatitis B core protein capsid [E. coli] Bottcher & Nassal 22 EMDB: EMD-0272 PDB: 6HU4 Cryo-EM structure: HBV pgRNA T = 4 NCP icosahedral symmetry [E. coli] Patel et al. 59 EMDB: EMD-11700 PDB: 7ABL X-ray crystallography structure: Human Hepatitis B Virus T = 4 capsid, strain adyw Bourne & Zlotnick 10 PDB: 2G33 X-ray crystallography structure: Human Hepatitis B viral capsid (HBcAg) [E. coli] Wynne et al. 19 PDB: 1QGT Experimental models: cell lines Human embryonic kidney 293T cells Luo et al. 34 N/A Recombinant DNA Plasmid for HEK-293T Cp183 wildtype HBV capsid: pCI-HBc Ludgate et al. 23 N/A Plasmid for HEK-293T Cp183 3A mutant capsid: pCI-HBc-3A Ludgate et al. 23 N/A Plasmid for E. coli Cp149 HBV capsids: pET11a-Cp149 Zlotnick et al. 60 N/A Plasmid for E. coli host RNA-filled Cp183 HBV capsids: pET11c-Cp183 Porterfield et al. 18 N/A Software and algorithms RELION v3.1 Zivanov et al. 61 https://relion.readthedocs.io/en/release-3.
Mutagenesis:Article Title: Cryo-EM structures of HBV capsids from human cells at near-atomic resolution.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-HBcAg (HBV core antigen) Zeta Corporation Cat #Z2085RL-A Goat anti-Rabbit Ig, human ads-HRP Southern Biotech Cat #4010-05; RRID: AB_2632593 Bacterial and virus strains Stellar TM Escherichia coli HST08 competent cells Takara Bio Cat #636763 E. coli BL21-CodonPlus (DE3) cells New England BioLabs Cat #C2527H Chemicals, peptides, and recombinant proteins DMEM/F12 cell culture medium Gibco Cat #11320-033 Hank’s Balanced Salt Solution (HBSS) (1X) Gibco Cat #14175-095 Penicillin-Streptomycin Solution Cytiva Cat #SV30010 HyClone TM Trypsin 0.25% (1X) Solution Cytiva Cat #SH30042 HyClone TM Fetal Bovine Serum Cytiva Cat #SH30071 YT – Broth Agar (capsules) MP Biomedicals Cat #30112 YT – Broth (powder) MP Biomedicals Cat #3011032 Ampicillin Sigma-Aldrich Cat #A9393 Carbenicillin disodium salt Chem-Impex International Inc. Cat #00049 Sucrose Sigma-Aldrich Cat #S0389 NP-40 core lysis buffer Nguyen & Hu 58 N/A Agarose Sigma-Aldrich Cat #A9539 UltraPure TM 10X TAE Buffer Invitrogen Cat #15558-026 Triton TM X-100 Sigma Life Science Cat #X100 Dialysis buffer This paper and Wang et al. 28 N/A SYPRO® Ruby Protein Gel Stain Lonza Cat #50564 SYBR TM Gold Nucleic Acid Gel Stain (10,000X concentrate in DMSO) Invitrogen Cat #S11494 Critical commercial assays NucleoBond® Xtra Maxi EF DNA purification kit MACHERY-NAGEL Cat #740424 CalPhos TM Mammalian Transfection Kit Takara Bio Cat #631312 Amersham TM ECL TM Western Blotting Detection Reagents Cytiva Cat #RPN2106 Deposited data Cryo-EM structure: HEK-293T Cp183 wildtype T = 4 capsid This paper EMDB: EMD-42823 PDB: 8UYT Cryo-EM structure: HEK-293T Cp183 wildtype T = 3 capsid This paper EMDB: EMD-42822 PDB: 8UYU Cryo-EM structure: HEK-293T Cp183 3A mutant T = 4 capsid This paper EMDB: EMD-42824 PDB: 8UYV Cryo-EM structure: HEK-293T Cp183 3A mutant T = 3 capsid This paper EMDB: EMD-42825 PDB: 8UYW Cryo-EM structure: pre-assembled E. coli Cp149 T = 4 capsid with TX-100 This paper EMDB: EMD-42827 PDB: 8UYY Cryo-EM structure: E. coli Cp149 T = 4 capsid co-assembled with TX-100 This paper EMDB: EMD-42828 PDB: 8UYZ (Continued on next page) Structure 34, 1–14.e1–e5, February 5, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-EM structure: E. coli Cp149 T = 4 capsid (wild-type control) This paper EMDB: EMD-42826 PDB: 8UYX Cryo-EM structure of HBV core [E. coli] Yu et al. 21 EMDB: EMD-2278 PDB: 3J2V Cryo-EM structure: F97L Hepatitis B core protein capsid [E. coli] Bottcher & Nassal 22 EMDB: EMD-0272 PDB: 6HU4 Cryo-EM structure: HBV pgRNA T = 4 NCP icosahedral symmetry [E. coli] Patel et al. 59 EMDB: EMD-11700 PDB: 7ABL X-ray crystallography structure: Human Hepatitis B Virus T = 4 capsid, strain adyw Bourne & Zlotnick 10 PDB: 2G33 X-ray crystallography structure: Human Hepatitis B viral capsid (HBcAg) [E. coli] Wynne et al. 19 PDB: 1QGT Experimental models: cell lines Human embryonic kidney 293T cells Luo et al. 34 N/A Recombinant DNA Plasmid for HEK-293T Cp183 wildtype HBV capsid: pCI-HBc Ludgate et al. 23 N/A Plasmid for HEK-293T Cp183 3A mutant capsid: pCI-HBc-3A Ludgate et al. 23 N/A Plasmid for E. coli Cp149 HBV capsids: pET11a-Cp149 Zlotnick et al. 60 N/A Plasmid for E. coli host RNA-filled Cp183 HBV capsids: pET11c-Cp183 Porterfield et al. 18 N/A Software and algorithms RELION v3.1 Zivanov et al. 61 https://relion.readthedocs.io/en/release-3.
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