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Valiant Co Ltd p nitrophenyl phosphate
P Nitrophenyl Phosphate, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/02100878%2E5/P-Nitrophenyl+phosphate/pmc04117635-229-10-12
Average 96 stars, based on 40 article reviews
p nitrophenyl phosphate - by Bioz Stars, 2026-09
96/100 stars

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Article Title: Vacuolar Phosphatidylinositol 3,4,5-trisphosphate controls fusion through binding Vam7, and membrane microdomain assembly
Article Snippet: C8-PI3P (1,2-dioctanoyl-phosphatidylinositol 3-phosphate), C8-PI(3,5)P 2 (1,2-dioctanoyl-phosphatidylinositol 3,5-bisphosphate), C8-PI(4,5)P 2 (1,2-dioctanoyl-phosphatidylinositol 4,5-bisphosphate), C8-PI(3,4,5)P 3 (1,2-dioctanoyl-phosphatidylinositol 3,4,5-trisphosphate), biotin-PI3P (b-PI3P), b-PI(3,4,5)P 3 , Inositol-1,3,4-trisphosphate (Ins(1,3,4)P 3 ), Inositol-1,3,5-trisphosphate (Ins(1,3,5)P 3 ), and Inositol-1,3,4,5-tetraphosphate (Ins(1,3,4,5)P 4 ) were from Echelon (Salt Lake City UT). p -nitrophenyl phosphate was from MP Biomedicals (Santa Ana, Ca).

Article Title: Macrophages direct location-dependent recall of B cell memory to vaccination.
Article Snippet: The absorbance of the reaction was read at 405 nm on a microplate reader (BioTek Instruments).

Incubation:

Article Title: Sphingolipids containing very long-chain fatty acids regulate Ypt7 function during the tethering stage of vacuole fusion.
Article Snippet: Content mixing reactions (30 μL) contained 3 μg each of vacuoles from BJ3505 (pep4∆ PHO8) and DKY6281 (PEP4 pho8∆) backgrounds in fusion reaction buffer consisting of PIPES-sorbitol (PS) buffer (20 mM PIPES-KOH, pH 6.8, 200 mM sorbitol), 125 mM KCl, 5 mM MgCl2, ATP regenerating system (1 mM ATP, 0.1 mg/mL creatine kinase [Sigma], 29 mM creatine phosphate [Gold Biotech]), 10 μM CoA, and 280 nM recombinant Pbi2 (IB2, Protease B inhibitor)(126). .. Reactions were incubated at 27°C and Pho8 activity was assayed in 250 mM Tris-Cl, pH 8.5, 0.4% Triton X100, 10 mM MgCl2, 1 mM p-nitrophenyl phosphate (MP Biomedicals). ..

Article Title: Sphingolipids containing very long-chain fatty acids regulate Ypt7 function during the tethering stage of vacuole fusion
Article Snippet: Content mixing reactions (30 μl) contained 3 μg each of vacuoles from BJ3505 ( pep4 Δ PHO8 ) and DKY6281 ( PEP4 pho8 Δ) backgrounds in fusion reaction buffer consisting of PIPES-sorbitol (PS) buffer (20 mM PIPES-KOH, pH 6.8, 200 mM sorbitol), 125 mM KCl, 5 mM MgCl 2 , ATP regenerating system (1 mM ATP, 0.1 mg/ml creatine kinase [Sigma], 29 mM creatine phosphate [Gold Biotech]), 10 μM CoA, and 280 nM recombinant Pbi2 (IB 2 , Protease B inhibitor) ( ). .. Reactions were incubated at 27 °C and Pho8 activity was assayed in 250 mM Tris-Cl, pH 8.5, 0.4% Triton X-100, 10 mM MgCl 2 , 1 mM p -nitrophenyl phosphate (MP Biomedicals). ..

Activity Assay:

Article Title: Sphingolipids containing very long-chain fatty acids regulate Ypt7 function during the tethering stage of vacuole fusion.
Article Snippet: Content mixing reactions (30 μL) contained 3 μg each of vacuoles from BJ3505 (pep4∆ PHO8) and DKY6281 (PEP4 pho8∆) backgrounds in fusion reaction buffer consisting of PIPES-sorbitol (PS) buffer (20 mM PIPES-KOH, pH 6.8, 200 mM sorbitol), 125 mM KCl, 5 mM MgCl2, ATP regenerating system (1 mM ATP, 0.1 mg/mL creatine kinase [Sigma], 29 mM creatine phosphate [Gold Biotech]), 10 μM CoA, and 280 nM recombinant Pbi2 (IB2, Protease B inhibitor)(126). .. Reactions were incubated at 27°C and Pho8 activity was assayed in 250 mM Tris-Cl, pH 8.5, 0.4% Triton X100, 10 mM MgCl2, 1 mM p-nitrophenyl phosphate (MP Biomedicals). ..

Article Title: Sphingolipids containing very long-chain fatty acids regulate Ypt7 function during the tethering stage of vacuole fusion
Article Snippet: Content mixing reactions (30 μl) contained 3 μg each of vacuoles from BJ3505 ( pep4 Δ PHO8 ) and DKY6281 ( PEP4 pho8 Δ) backgrounds in fusion reaction buffer consisting of PIPES-sorbitol (PS) buffer (20 mM PIPES-KOH, pH 6.8, 200 mM sorbitol), 125 mM KCl, 5 mM MgCl 2 , ATP regenerating system (1 mM ATP, 0.1 mg/ml creatine kinase [Sigma], 29 mM creatine phosphate [Gold Biotech]), 10 μM CoA, and 280 nM recombinant Pbi2 (IB 2 , Protease B inhibitor) ( ). .. Reactions were incubated at 27 °C and Pho8 activity was assayed in 250 mM Tris-Cl, pH 8.5, 0.4% Triton X-100, 10 mM MgCl 2 , 1 mM p -nitrophenyl phosphate (MP Biomedicals). ..



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