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l arabinose  (Valiant Co Ltd)

 
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    Valiant Co Ltd l arabinose
    L Arabinose, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/0210070625/L-(%2B)-Arabinose/10__1038_slash_nprot__2013__081-65-86-89
    Average 92 stars, based on 19 article reviews
    l arabinose - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Bacteria:

    Article Title: An integrated, peptide-based approach to site-specific protein immobilization for detection of biomolecular interactions.
    Article Snippet: .. The bacteria were cultured at 37 °C and expression was induced (at OD 0.7) using 0.1% of L-arabinose and 0.5 mM isopropyl β-D-1-thiogalactopyranoside (MP Biomedicals, Santa Ana, CA, USA), after which the bacteria were cultured overnight at 25 °C. ..

    Cell Culture:

    Article Title: An integrated, peptide-based approach to site-specific protein immobilization for detection of biomolecular interactions.
    Article Snippet: .. The bacteria were cultured at 37 °C and expression was induced (at OD 0.7) using 0.1% of L-arabinose and 0.5 mM isopropyl β-D-1-thiogalactopyranoside (MP Biomedicals, Santa Ana, CA, USA), after which the bacteria were cultured overnight at 25 °C. ..

    Expressing:

    Article Title: An integrated, peptide-based approach to site-specific protein immobilization for detection of biomolecular interactions.
    Article Snippet: .. The bacteria were cultured at 37 °C and expression was induced (at OD 0.7) using 0.1% of L-arabinose and 0.5 mM isopropyl β-D-1-thiogalactopyranoside (MP Biomedicals, Santa Ana, CA, USA), after which the bacteria were cultured overnight at 25 °C. ..

    Concentration Assay:

    Article Title: Decline in nitrosative stress drives antibiotic persister regrowth during infection.
    Article Snippet: Article Decline in nitrosative stress drives antibiotic persister regrowth during infection

    Infection:

    Article Title: Decline in nitrosative stress drives antibiotic persister regrowth during infection.
    Article Snippet: Article Decline in nitrosative stress drives antibiotic persister regrowth during infection

    Virus:

    Article Title: N6-Methyladenosine Modification Controls Circular RNA Immunity.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-EspA-polyclonal, rabbit UT Southwestern, Vanessa Sperandio N/A Anti-EspB-polyclonal, rabbit UT Southwestern, Vanessa Sperandio N/A Anti-rabbit-HRP, goat BioRad Cat# 1706515; RRID: AB_2617112 Bacterial and virus strains E. coli HS Center for Vaccine Development at the University of Maryland School of Medicine N/A C. rodentium DBS100 Tufts University, John Leong N/A Chemicals, peptides, and recombinant proteins Dulbecco eagle medium, low glucose Invitrogen Cat# 11885-084 Cocaine Hydrochloride Sigma-Aldrich Cat#C5776 LB agar, Miller Fisher Scientific Cat# BP1425 LB broth, Miller Fisher Scientific Cat# BP9723 Ampicillin powder Sigma-Aldrich Cat# A9518 Naladixic acid power Fisher Scientific Cat# BP908 L-(+)-Arabinose Sigma-Aldrich Cat# A3256 Dextran sulfate sodium salt MP Biomedicals Cat#021601109 Phusion High fidelity PCR master mix Thermo Fisher Cat# F-531L Random primers Invitrogen Cat# 48190-011 Super script II reverse transcriptase Invitrogen Cat# 18064-014 Sybr green master mix Thermo Fisher Cat# 4309155 Sarcosine Sigma-Aldrich Cat#131776 Glycine Hydrochloride Sigma-Aldrich Cat# G2879 Trizol reagent Thermo Fisher Cat# 1559618 Chloroform IBM Scientific Cat# 67-66-3 dNTP mix 10mM Invitrogen Cat# 18-427-088 Bovine Serum Albumin Sigma-Aldrich Cat# A2153 Dopamine Hydrochloride Sigma-Aldrich Cat# H8502 6-hydroxydopamine Hydrochloride Sigma-Aldrich Cat# H4381 RNAlater-ICE Fisher Scientific Cat# 44-275-75 Protease inhibitor cocktail Sigma-Aldrich Cat# P8849-5 Dithiothreitol (DTT) Fisher Scientific Cat# BP172-25 Formaldehyde Fisher Scientific Cat# BP539 Brain heart infusion agar Becton Dickinson Cat# 2418102 Dextran sulfate sodium salt Fisher Scientific Cat# 02-160-1109 Metronidazole MP biomedicals Cat# 0215571025 Neomycin trisulfate salt hydrate Sigma-Aldrich Cat# N5285 Vancomycin hydrochloride Research Products International Cat# V06500 Critical Commercial Assays RNeasy Power Microbiome Qiagen Cat# 26000-50 RNeasy Plus Mini Kit Qiagen Cat# 74134 RiboPure RNA isolation Kit Ambion Cat# 1405072 GenElute Plasmid Miniprep Sigma Cat# PLN350-1KT QIAquick PCR purification kit Qiagen Cat# 28906 Glycine Assay Kit Sigma-Aldrich Cat# MAK261 (Continued on next page) e1 Cell Host & Microbe 30, 1615–1629.e1–e5, November 9, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA sequencing data https://www.ncbi.nlm.nih.gov/geo/ GSE212421 Experimental models: Organisms/strains C57BL/6J mice The Jackson laboratory Cat# 000664 Oligonucleotides Primers for qRT-PCR used in this study are listed in Table S1 This paper N/A Software and algorithms Prism 6 GraphPad N/A QuantStudio real-time PCR software v1.3 Thermo Fisher N/A Image Lab 5.2.1 BioRad N/A iDEP.91 http://bioinformatics.sdstate.edu/idep/ N/A CLC Bio microbial genomics module https://www.qiagenbioinformatics.com/ plugins/clc-microbial-genomics-module/ N/A MetaboAnalyst 5.0 https://www.metaboanalyst.ca/ N/A Fiji Image J https://fiji.sc/ N/A Fusion 6.5v Omnitech Electronics Incorporated N/A MultiQuant 3.0.3.

    Bioprocessing:

    Article Title: N6-Methyladenosine Modification Controls Circular RNA Immunity.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-EspA-polyclonal, rabbit UT Southwestern, Vanessa Sperandio N/A Anti-EspB-polyclonal, rabbit UT Southwestern, Vanessa Sperandio N/A Anti-rabbit-HRP, goat BioRad Cat# 1706515; RRID: AB_2617112 Bacterial and virus strains E. coli HS Center for Vaccine Development at the University of Maryland School of Medicine N/A C. rodentium DBS100 Tufts University, John Leong N/A Chemicals, peptides, and recombinant proteins Dulbecco eagle medium, low glucose Invitrogen Cat# 11885-084 Cocaine Hydrochloride Sigma-Aldrich Cat#C5776 LB agar, Miller Fisher Scientific Cat# BP1425 LB broth, Miller Fisher Scientific Cat# BP9723 Ampicillin powder Sigma-Aldrich Cat# A9518 Naladixic acid power Fisher Scientific Cat# BP908 L-(+)-Arabinose Sigma-Aldrich Cat# A3256 Dextran sulfate sodium salt MP Biomedicals Cat#021601109 Phusion High fidelity PCR master mix Thermo Fisher Cat# F-531L Random primers Invitrogen Cat# 48190-011 Super script II reverse transcriptase Invitrogen Cat# 18064-014 Sybr green master mix Thermo Fisher Cat# 4309155 Sarcosine Sigma-Aldrich Cat#131776 Glycine Hydrochloride Sigma-Aldrich Cat# G2879 Trizol reagent Thermo Fisher Cat# 1559618 Chloroform IBM Scientific Cat# 67-66-3 dNTP mix 10mM Invitrogen Cat# 18-427-088 Bovine Serum Albumin Sigma-Aldrich Cat# A2153 Dopamine Hydrochloride Sigma-Aldrich Cat# H8502 6-hydroxydopamine Hydrochloride Sigma-Aldrich Cat# H4381 RNAlater-ICE Fisher Scientific Cat# 44-275-75 Protease inhibitor cocktail Sigma-Aldrich Cat# P8849-5 Dithiothreitol (DTT) Fisher Scientific Cat# BP172-25 Formaldehyde Fisher Scientific Cat# BP539 Brain heart infusion agar Becton Dickinson Cat# 2418102 Dextran sulfate sodium salt Fisher Scientific Cat# 02-160-1109 Metronidazole MP biomedicals Cat# 0215571025 Neomycin trisulfate salt hydrate Sigma-Aldrich Cat# N5285 Vancomycin hydrochloride Research Products International Cat# V06500 Critical Commercial Assays RNeasy Power Microbiome Qiagen Cat# 26000-50 RNeasy Plus Mini Kit Qiagen Cat# 74134 RiboPure RNA isolation Kit Ambion Cat# 1405072 GenElute Plasmid Miniprep Sigma Cat# PLN350-1KT QIAquick PCR purification kit Qiagen Cat# 28906 Glycine Assay Kit Sigma-Aldrich Cat# MAK261 (Continued on next page) e1 Cell Host & Microbe 30, 1615–1629.e1–e5, November 9, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA sequencing data https://www.ncbi.nlm.nih.gov/geo/ GSE212421 Experimental models: Organisms/strains C57BL/6J mice The Jackson laboratory Cat# 000664 Oligonucleotides Primers for qRT-PCR used in this study are listed in Table S1 This paper N/A Software and algorithms Prism 6 GraphPad N/A QuantStudio real-time PCR software v1.3 Thermo Fisher N/A Image Lab 5.2.1 BioRad N/A iDEP.91 http://bioinformatics.sdstate.edu/idep/ N/A CLC Bio microbial genomics module https://www.qiagenbioinformatics.com/ plugins/clc-microbial-genomics-module/ N/A MetaboAnalyst 5.0 https://www.metaboanalyst.ca/ N/A Fiji Image J https://fiji.sc/ N/A Fusion 6.5v Omnitech Electronics Incorporated N/A MultiQuant 3.0.3.

    Recombinant:

    Article Title: N6-Methyladenosine Modification Controls Circular RNA Immunity.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-EspA-polyclonal, rabbit UT Southwestern, Vanessa Sperandio N/A Anti-EspB-polyclonal, rabbit UT Southwestern, Vanessa Sperandio N/A Anti-rabbit-HRP, goat BioRad Cat# 1706515; RRID: AB_2617112 Bacterial and virus strains E. coli HS Center for Vaccine Development at the University of Maryland School of Medicine N/A C. rodentium DBS100 Tufts University, John Leong N/A Chemicals, peptides, and recombinant proteins Dulbecco eagle medium, low glucose Invitrogen Cat# 11885-084 Cocaine Hydrochloride Sigma-Aldrich Cat#C5776 LB agar, Miller Fisher Scientific Cat# BP1425 LB broth, Miller Fisher Scientific Cat# BP9723 Ampicillin powder Sigma-Aldrich Cat# A9518 Naladixic acid power Fisher Scientific Cat# BP908 L-(+)-Arabinose Sigma-Aldrich Cat# A3256 Dextran sulfate sodium salt MP Biomedicals Cat#021601109 Phusion High fidelity PCR master mix Thermo Fisher Cat# F-531L Random primers Invitrogen Cat# 48190-011 Super script II reverse transcriptase Invitrogen Cat# 18064-014 Sybr green master mix Thermo Fisher Cat# 4309155 Sarcosine Sigma-Aldrich Cat#131776 Glycine Hydrochloride Sigma-Aldrich Cat# G2879 Trizol reagent Thermo Fisher Cat# 1559618 Chloroform IBM Scientific Cat# 67-66-3 dNTP mix 10mM Invitrogen Cat# 18-427-088 Bovine Serum Albumin Sigma-Aldrich Cat# A2153 Dopamine Hydrochloride Sigma-Aldrich Cat# H8502 6-hydroxydopamine Hydrochloride Sigma-Aldrich Cat# H4381 RNAlater-ICE Fisher Scientific Cat# 44-275-75 Protease inhibitor cocktail Sigma-Aldrich Cat# P8849-5 Dithiothreitol (DTT) Fisher Scientific Cat# BP172-25 Formaldehyde Fisher Scientific Cat# BP539 Brain heart infusion agar Becton Dickinson Cat# 2418102 Dextran sulfate sodium salt Fisher Scientific Cat# 02-160-1109 Metronidazole MP biomedicals Cat# 0215571025 Neomycin trisulfate salt hydrate Sigma-Aldrich Cat# N5285 Vancomycin hydrochloride Research Products International Cat# V06500 Critical Commercial Assays RNeasy Power Microbiome Qiagen Cat# 26000-50 RNeasy Plus Mini Kit Qiagen Cat# 74134 RiboPure RNA isolation Kit Ambion Cat# 1405072 GenElute Plasmid Miniprep Sigma Cat# PLN350-1KT QIAquick PCR purification kit Qiagen Cat# 28906 Glycine Assay Kit Sigma-Aldrich Cat# MAK261 (Continued on next page) e1 Cell Host & Microbe 30, 1615–1629.e1–e5, November 9, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA sequencing data https://www.ncbi.nlm.nih.gov/geo/ GSE212421 Experimental models: Organisms/strains C57BL/6J mice The Jackson laboratory Cat# 000664 Oligonucleotides Primers for qRT-PCR used in this study are listed in Table S1 This paper N/A Software and algorithms Prism 6 GraphPad N/A QuantStudio real-time PCR software v1.3 Thermo Fisher N/A Image Lab 5.2.1 BioRad N/A iDEP.91 http://bioinformatics.sdstate.edu/idep/ N/A CLC Bio microbial genomics module https://www.qiagenbioinformatics.com/ plugins/clc-microbial-genomics-module/ N/A MetaboAnalyst 5.0 https://www.metaboanalyst.ca/ N/A Fiji Image J https://fiji.sc/ N/A Fusion 6.5v Omnitech Electronics Incorporated N/A MultiQuant 3.0.3.

    Polymerase Chain Reaction:

    Article Title: N6-Methyladenosine Modification Controls Circular RNA Immunity.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-EspA-polyclonal, rabbit UT Southwestern, Vanessa Sperandio N/A Anti-EspB-polyclonal, rabbit UT Southwestern, Vanessa Sperandio N/A Anti-rabbit-HRP, goat BioRad Cat# 1706515; RRID: AB_2617112 Bacterial and virus strains E. coli HS Center for Vaccine Development at the University of Maryland School of Medicine N/A C. rodentium DBS100 Tufts University, John Leong N/A Chemicals, peptides, and recombinant proteins Dulbecco eagle medium, low glucose Invitrogen Cat# 11885-084 Cocaine Hydrochloride Sigma-Aldrich Cat#C5776 LB agar, Miller Fisher Scientific Cat# BP1425 LB broth, Miller Fisher Scientific Cat# BP9723 Ampicillin powder Sigma-Aldrich Cat# A9518 Naladixic acid power Fisher Scientific Cat# BP908 L-(+)-Arabinose Sigma-Aldrich Cat# A3256 Dextran sulfate sodium salt MP Biomedicals Cat#021601109 Phusion High fidelity PCR master mix Thermo Fisher Cat# F-531L Random primers Invitrogen Cat# 48190-011 Super script II reverse transcriptase Invitrogen Cat# 18064-014 Sybr green master mix Thermo Fisher Cat# 4309155 Sarcosine Sigma-Aldrich Cat#131776 Glycine Hydrochloride Sigma-Aldrich Cat# G2879 Trizol reagent Thermo Fisher Cat# 1559618 Chloroform IBM Scientific Cat# 67-66-3 dNTP mix 10mM Invitrogen Cat# 18-427-088 Bovine Serum Albumin Sigma-Aldrich Cat# A2153 Dopamine Hydrochloride Sigma-Aldrich Cat# H8502 6-hydroxydopamine Hydrochloride Sigma-Aldrich Cat# H4381 RNAlater-ICE Fisher Scientific Cat# 44-275-75 Protease inhibitor cocktail Sigma-Aldrich Cat# P8849-5 Dithiothreitol (DTT) Fisher Scientific Cat# BP172-25 Formaldehyde Fisher Scientific Cat# BP539 Brain heart infusion agar Becton Dickinson Cat# 2418102 Dextran sulfate sodium salt Fisher Scientific Cat# 02-160-1109 Metronidazole MP biomedicals Cat# 0215571025 Neomycin trisulfate salt hydrate Sigma-Aldrich Cat# N5285 Vancomycin hydrochloride Research Products International Cat# V06500 Critical Commercial Assays RNeasy Power Microbiome Qiagen Cat# 26000-50 RNeasy Plus Mini Kit Qiagen Cat# 74134 RiboPure RNA isolation Kit Ambion Cat# 1405072 GenElute Plasmid Miniprep Sigma Cat# PLN350-1KT QIAquick PCR purification kit Qiagen Cat# 28906 Glycine Assay Kit Sigma-Aldrich Cat# MAK261 (Continued on next page) e1 Cell Host & Microbe 30, 1615–1629.e1–e5, November 9, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA sequencing data https://www.ncbi.nlm.nih.gov/geo/ GSE212421 Experimental models: Organisms/strains C57BL/6J mice The Jackson laboratory Cat# 000664 Oligonucleotides Primers for qRT-PCR used in this study are listed in Table S1 This paper N/A Software and algorithms Prism 6 GraphPad N/A QuantStudio real-time PCR software v1.3 Thermo Fisher N/A Image Lab 5.2.1 BioRad N/A iDEP.91 http://bioinformatics.sdstate.edu/idep/ N/A CLC Bio microbial genomics module https://www.qiagenbioinformatics.com/ plugins/clc-microbial-genomics-module/ N/A MetaboAnalyst 5.0 https://www.metaboanalyst.ca/ N/A Fiji Image J https://fiji.sc/ N/A Fusion 6.5v Omnitech Electronics Incorporated N/A MultiQuant 3.0.3.

    Reverse Transcription:

    Article Title: N6-Methyladenosine Modification Controls Circular RNA Immunity.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-EspA-polyclonal, rabbit UT Southwestern, Vanessa Sperandio N/A Anti-EspB-polyclonal, rabbit UT Southwestern, Vanessa Sperandio N/A Anti-rabbit-HRP, goat BioRad Cat# 1706515; RRID: AB_2617112 Bacterial and virus strains E. coli HS Center for Vaccine Development at the University of Maryland School of Medicine N/A C. rodentium DBS100 Tufts University, John Leong N/A Chemicals, peptides, and recombinant proteins Dulbecco eagle medium, low glucose Invitrogen Cat# 11885-084 Cocaine Hydrochloride Sigma-Aldrich Cat#C5776 LB agar, Miller Fisher Scientific Cat# BP1425 LB broth, Miller Fisher Scientific Cat# BP9723 Ampicillin powder Sigma-Aldrich Cat# A9518 Naladixic acid power Fisher Scientific Cat# BP908 L-(+)-Arabinose Sigma-Aldrich Cat# A3256 Dextran sulfate sodium salt MP Biomedicals Cat#021601109 Phusion High fidelity PCR master mix Thermo Fisher Cat# F-531L Random primers Invitrogen Cat# 48190-011 Super script II reverse transcriptase Invitrogen Cat# 18064-014 Sybr green master mix Thermo Fisher Cat# 4309155 Sarcosine Sigma-Aldrich Cat#131776 Glycine Hydrochloride Sigma-Aldrich Cat# G2879 Trizol reagent Thermo Fisher Cat# 1559618 Chloroform IBM Scientific Cat# 67-66-3 dNTP mix 10mM Invitrogen Cat# 18-427-088 Bovine Serum Albumin Sigma-Aldrich Cat# A2153 Dopamine Hydrochloride Sigma-Aldrich Cat# H8502 6-hydroxydopamine Hydrochloride Sigma-Aldrich Cat# H4381 RNAlater-ICE Fisher Scientific Cat# 44-275-75 Protease inhibitor cocktail Sigma-Aldrich Cat# P8849-5 Dithiothreitol (DTT) Fisher Scientific Cat# BP172-25 Formaldehyde Fisher Scientific Cat# BP539 Brain heart infusion agar Becton Dickinson Cat# 2418102 Dextran sulfate sodium salt Fisher Scientific Cat# 02-160-1109 Metronidazole MP biomedicals Cat# 0215571025 Neomycin trisulfate salt hydrate Sigma-Aldrich Cat# N5285 Vancomycin hydrochloride Research Products International Cat# V06500 Critical Commercial Assays RNeasy Power Microbiome Qiagen Cat# 26000-50 RNeasy Plus Mini Kit Qiagen Cat# 74134 RiboPure RNA isolation Kit Ambion Cat# 1405072 GenElute Plasmid Miniprep Sigma Cat# PLN350-1KT QIAquick PCR purification kit Qiagen Cat# 28906 Glycine Assay Kit Sigma-Aldrich Cat# MAK261 (Continued on next page) e1 Cell Host & Microbe 30, 1615–1629.e1–e5, November 9, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA sequencing data https://www.ncbi.nlm.nih.gov/geo/ GSE212421 Experimental models: Organisms/strains C57BL/6J mice The Jackson laboratory Cat# 000664 Oligonucleotides Primers for qRT-PCR used in this study are listed in Table S1 This paper N/A Software and algorithms Prism 6 GraphPad N/A QuantStudio real-time PCR software v1.3 Thermo Fisher N/A Image Lab 5.2.1 BioRad N/A iDEP.91 http://bioinformatics.sdstate.edu/idep/ N/A CLC Bio microbial genomics module https://www.qiagenbioinformatics.com/ plugins/clc-microbial-genomics-module/ N/A MetaboAnalyst 5.0 https://www.metaboanalyst.ca/ N/A Fiji Image J https://fiji.sc/ N/A Fusion 6.5v Omnitech Electronics Incorporated N/A MultiQuant 3.0.3.

    SYBR Green Assay:

    Article Title: N6-Methyladenosine Modification Controls Circular RNA Immunity.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-EspA-polyclonal, rabbit UT Southwestern, Vanessa Sperandio N/A Anti-EspB-polyclonal, rabbit UT Southwestern, Vanessa Sperandio N/A Anti-rabbit-HRP, goat BioRad Cat# 1706515; RRID: AB_2617112 Bacterial and virus strains E. coli HS Center for Vaccine Development at the University of Maryland School of Medicine N/A C. rodentium DBS100 Tufts University, John Leong N/A Chemicals, peptides, and recombinant proteins Dulbecco eagle medium, low glucose Invitrogen Cat# 11885-084 Cocaine Hydrochloride Sigma-Aldrich Cat#C5776 LB agar, Miller Fisher Scientific Cat# BP1425 LB broth, Miller Fisher Scientific Cat# BP9723 Ampicillin powder Sigma-Aldrich Cat# A9518 Naladixic acid power Fisher Scientific Cat# BP908 L-(+)-Arabinose Sigma-Aldrich Cat# A3256 Dextran sulfate sodium salt MP Biomedicals Cat#021601109 Phusion High fidelity PCR master mix Thermo Fisher Cat# F-531L Random primers Invitrogen Cat# 48190-011 Super script II reverse transcriptase Invitrogen Cat# 18064-014 Sybr green master mix Thermo Fisher Cat# 4309155 Sarcosine Sigma-Aldrich Cat#131776 Glycine Hydrochloride Sigma-Aldrich Cat# G2879 Trizol reagent Thermo Fisher Cat# 1559618 Chloroform IBM Scientific Cat# 67-66-3 dNTP mix 10mM Invitrogen Cat# 18-427-088 Bovine Serum Albumin Sigma-Aldrich Cat# A2153 Dopamine Hydrochloride Sigma-Aldrich Cat# H8502 6-hydroxydopamine Hydrochloride Sigma-Aldrich Cat# H4381 RNAlater-ICE Fisher Scientific Cat# 44-275-75 Protease inhibitor cocktail Sigma-Aldrich Cat# P8849-5 Dithiothreitol (DTT) Fisher Scientific Cat# BP172-25 Formaldehyde Fisher Scientific Cat# BP539 Brain heart infusion agar Becton Dickinson Cat# 2418102 Dextran sulfate sodium salt Fisher Scientific Cat# 02-160-1109 Metronidazole MP biomedicals Cat# 0215571025 Neomycin trisulfate salt hydrate Sigma-Aldrich Cat# N5285 Vancomycin hydrochloride Research Products International Cat# V06500 Critical Commercial Assays RNeasy Power Microbiome Qiagen Cat# 26000-50 RNeasy Plus Mini Kit Qiagen Cat# 74134 RiboPure RNA isolation Kit Ambion Cat# 1405072 GenElute Plasmid Miniprep Sigma Cat# PLN350-1KT QIAquick PCR purification kit Qiagen Cat# 28906 Glycine Assay Kit Sigma-Aldrich Cat# MAK261 (Continued on next page) e1 Cell Host & Microbe 30, 1615–1629.e1–e5, November 9, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA sequencing data https://www.ncbi.nlm.nih.gov/geo/ GSE212421 Experimental models: Organisms/strains C57BL/6J mice The Jackson laboratory Cat# 000664 Oligonucleotides Primers for qRT-PCR used in this study are listed in Table S1 This paper N/A Software and algorithms Prism 6 GraphPad N/A QuantStudio real-time PCR software v1.3 Thermo Fisher N/A Image Lab 5.2.1 BioRad N/A iDEP.91 http://bioinformatics.sdstate.edu/idep/ N/A CLC Bio microbial genomics module https://www.qiagenbioinformatics.com/ plugins/clc-microbial-genomics-module/ N/A MetaboAnalyst 5.0 https://www.metaboanalyst.ca/ N/A Fiji Image J https://fiji.sc/ N/A Fusion 6.5v Omnitech Electronics Incorporated N/A MultiQuant 3.0.3.

    Protease Inhibitor:

    Article Title: N6-Methyladenosine Modification Controls Circular RNA Immunity.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-EspA-polyclonal, rabbit UT Southwestern, Vanessa Sperandio N/A Anti-EspB-polyclonal, rabbit UT Southwestern, Vanessa Sperandio N/A Anti-rabbit-HRP, goat BioRad Cat# 1706515; RRID: AB_2617112 Bacterial and virus strains E. coli HS Center for Vaccine Development at the University of Maryland School of Medicine N/A C. rodentium DBS100 Tufts University, John Leong N/A Chemicals, peptides, and recombinant proteins Dulbecco eagle medium, low glucose Invitrogen Cat# 11885-084 Cocaine Hydrochloride Sigma-Aldrich Cat#C5776 LB agar, Miller Fisher Scientific Cat# BP1425 LB broth, Miller Fisher Scientific Cat# BP9723 Ampicillin powder Sigma-Aldrich Cat# A9518 Naladixic acid power Fisher Scientific Cat# BP908 L-(+)-Arabinose Sigma-Aldrich Cat# A3256 Dextran sulfate sodium salt MP Biomedicals Cat#021601109 Phusion High fidelity PCR master mix Thermo Fisher Cat# F-531L Random primers Invitrogen Cat# 48190-011 Super script II reverse transcriptase Invitrogen Cat# 18064-014 Sybr green master mix Thermo Fisher Cat# 4309155 Sarcosine Sigma-Aldrich Cat#131776 Glycine Hydrochloride Sigma-Aldrich Cat# G2879 Trizol reagent Thermo Fisher Cat# 1559618 Chloroform IBM Scientific Cat# 67-66-3 dNTP mix 10mM Invitrogen Cat# 18-427-088 Bovine Serum Albumin Sigma-Aldrich Cat# A2153 Dopamine Hydrochloride Sigma-Aldrich Cat# H8502 6-hydroxydopamine Hydrochloride Sigma-Aldrich Cat# H4381 RNAlater-ICE Fisher Scientific Cat# 44-275-75 Protease inhibitor cocktail Sigma-Aldrich Cat# P8849-5 Dithiothreitol (DTT) Fisher Scientific Cat# BP172-25 Formaldehyde Fisher Scientific Cat# BP539 Brain heart infusion agar Becton Dickinson Cat# 2418102 Dextran sulfate sodium salt Fisher Scientific Cat# 02-160-1109 Metronidazole MP biomedicals Cat# 0215571025 Neomycin trisulfate salt hydrate Sigma-Aldrich Cat# N5285 Vancomycin hydrochloride Research Products International Cat# V06500 Critical Commercial Assays RNeasy Power Microbiome Qiagen Cat# 26000-50 RNeasy Plus Mini Kit Qiagen Cat# 74134 RiboPure RNA isolation Kit Ambion Cat# 1405072 GenElute Plasmid Miniprep Sigma Cat# PLN350-1KT QIAquick PCR purification kit Qiagen Cat# 28906 Glycine Assay Kit Sigma-Aldrich Cat# MAK261 (Continued on next page) e1 Cell Host & Microbe 30, 1615–1629.e1–e5, November 9, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA sequencing data https://www.ncbi.nlm.nih.gov/geo/ GSE212421 Experimental models: Organisms/strains C57BL/6J mice The Jackson laboratory Cat# 000664 Oligonucleotides Primers for qRT-PCR used in this study are listed in Table S1 This paper N/A Software and algorithms Prism 6 GraphPad N/A QuantStudio real-time PCR software v1.3 Thermo Fisher N/A Image Lab 5.2.1 BioRad N/A iDEP.91 http://bioinformatics.sdstate.edu/idep/ N/A CLC Bio microbial genomics module https://www.qiagenbioinformatics.com/ plugins/clc-microbial-genomics-module/ N/A MetaboAnalyst 5.0 https://www.metaboanalyst.ca/ N/A Fiji Image J https://fiji.sc/ N/A Fusion 6.5v Omnitech Electronics Incorporated N/A MultiQuant 3.0.3.

    Isolation:

    Article Title: N6-Methyladenosine Modification Controls Circular RNA Immunity.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-EspA-polyclonal, rabbit UT Southwestern, Vanessa Sperandio N/A Anti-EspB-polyclonal, rabbit UT Southwestern, Vanessa Sperandio N/A Anti-rabbit-HRP, goat BioRad Cat# 1706515; RRID: AB_2617112 Bacterial and virus strains E. coli HS Center for Vaccine Development at the University of Maryland School of Medicine N/A C. rodentium DBS100 Tufts University, John Leong N/A Chemicals, peptides, and recombinant proteins Dulbecco eagle medium, low glucose Invitrogen Cat# 11885-084 Cocaine Hydrochloride Sigma-Aldrich Cat#C5776 LB agar, Miller Fisher Scientific Cat# BP1425 LB broth, Miller Fisher Scientific Cat# BP9723 Ampicillin powder Sigma-Aldrich Cat# A9518 Naladixic acid power Fisher Scientific Cat# BP908 L-(+)-Arabinose Sigma-Aldrich Cat# A3256 Dextran sulfate sodium salt MP Biomedicals Cat#021601109 Phusion High fidelity PCR master mix Thermo Fisher Cat# F-531L Random primers Invitrogen Cat# 48190-011 Super script II reverse transcriptase Invitrogen Cat# 18064-014 Sybr green master mix Thermo Fisher Cat# 4309155 Sarcosine Sigma-Aldrich Cat#131776 Glycine Hydrochloride Sigma-Aldrich Cat# G2879 Trizol reagent Thermo Fisher Cat# 1559618 Chloroform IBM Scientific Cat# 67-66-3 dNTP mix 10mM Invitrogen Cat# 18-427-088 Bovine Serum Albumin Sigma-Aldrich Cat# A2153 Dopamine Hydrochloride Sigma-Aldrich Cat# H8502 6-hydroxydopamine Hydrochloride Sigma-Aldrich Cat# H4381 RNAlater-ICE Fisher Scientific Cat# 44-275-75 Protease inhibitor cocktail Sigma-Aldrich Cat# P8849-5 Dithiothreitol (DTT) Fisher Scientific Cat# BP172-25 Formaldehyde Fisher Scientific Cat# BP539 Brain heart infusion agar Becton Dickinson Cat# 2418102 Dextran sulfate sodium salt Fisher Scientific Cat# 02-160-1109 Metronidazole MP biomedicals Cat# 0215571025 Neomycin trisulfate salt hydrate Sigma-Aldrich Cat# N5285 Vancomycin hydrochloride Research Products International Cat# V06500 Critical Commercial Assays RNeasy Power Microbiome Qiagen Cat# 26000-50 RNeasy Plus Mini Kit Qiagen Cat# 74134 RiboPure RNA isolation Kit Ambion Cat# 1405072 GenElute Plasmid Miniprep Sigma Cat# PLN350-1KT QIAquick PCR purification kit Qiagen Cat# 28906 Glycine Assay Kit Sigma-Aldrich Cat# MAK261 (Continued on next page) e1 Cell Host & Microbe 30, 1615–1629.e1–e5, November 9, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA sequencing data https://www.ncbi.nlm.nih.gov/geo/ GSE212421 Experimental models: Organisms/strains C57BL/6J mice The Jackson laboratory Cat# 000664 Oligonucleotides Primers for qRT-PCR used in this study are listed in Table S1 This paper N/A Software and algorithms Prism 6 GraphPad N/A QuantStudio real-time PCR software v1.3 Thermo Fisher N/A Image Lab 5.2.1 BioRad N/A iDEP.91 http://bioinformatics.sdstate.edu/idep/ N/A CLC Bio microbial genomics module https://www.qiagenbioinformatics.com/ plugins/clc-microbial-genomics-module/ N/A MetaboAnalyst 5.0 https://www.metaboanalyst.ca/ N/A Fiji Image J https://fiji.sc/ N/A Fusion 6.5v Omnitech Electronics Incorporated N/A MultiQuant 3.0.3.

    Plasmid Preparation:

    Article Title: N6-Methyladenosine Modification Controls Circular RNA Immunity.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-EspA-polyclonal, rabbit UT Southwestern, Vanessa Sperandio N/A Anti-EspB-polyclonal, rabbit UT Southwestern, Vanessa Sperandio N/A Anti-rabbit-HRP, goat BioRad Cat# 1706515; RRID: AB_2617112 Bacterial and virus strains E. coli HS Center for Vaccine Development at the University of Maryland School of Medicine N/A C. rodentium DBS100 Tufts University, John Leong N/A Chemicals, peptides, and recombinant proteins Dulbecco eagle medium, low glucose Invitrogen Cat# 11885-084 Cocaine Hydrochloride Sigma-Aldrich Cat#C5776 LB agar, Miller Fisher Scientific Cat# BP1425 LB broth, Miller Fisher Scientific Cat# BP9723 Ampicillin powder Sigma-Aldrich Cat# A9518 Naladixic acid power Fisher Scientific Cat# BP908 L-(+)-Arabinose Sigma-Aldrich Cat# A3256 Dextran sulfate sodium salt MP Biomedicals Cat#021601109 Phusion High fidelity PCR master mix Thermo Fisher Cat# F-531L Random primers Invitrogen Cat# 48190-011 Super script II reverse transcriptase Invitrogen Cat# 18064-014 Sybr green master mix Thermo Fisher Cat# 4309155 Sarcosine Sigma-Aldrich Cat#131776 Glycine Hydrochloride Sigma-Aldrich Cat# G2879 Trizol reagent Thermo Fisher Cat# 1559618 Chloroform IBM Scientific Cat# 67-66-3 dNTP mix 10mM Invitrogen Cat# 18-427-088 Bovine Serum Albumin Sigma-Aldrich Cat# A2153 Dopamine Hydrochloride Sigma-Aldrich Cat# H8502 6-hydroxydopamine Hydrochloride Sigma-Aldrich Cat# H4381 RNAlater-ICE Fisher Scientific Cat# 44-275-75 Protease inhibitor cocktail Sigma-Aldrich Cat# P8849-5 Dithiothreitol (DTT) Fisher Scientific Cat# BP172-25 Formaldehyde Fisher Scientific Cat# BP539 Brain heart infusion agar Becton Dickinson Cat# 2418102 Dextran sulfate sodium salt Fisher Scientific Cat# 02-160-1109 Metronidazole MP biomedicals Cat# 0215571025 Neomycin trisulfate salt hydrate Sigma-Aldrich Cat# N5285 Vancomycin hydrochloride Research Products International Cat# V06500 Critical Commercial Assays RNeasy Power Microbiome Qiagen Cat# 26000-50 RNeasy Plus Mini Kit Qiagen Cat# 74134 RiboPure RNA isolation Kit Ambion Cat# 1405072 GenElute Plasmid Miniprep Sigma Cat# PLN350-1KT QIAquick PCR purification kit Qiagen Cat# 28906 Glycine Assay Kit Sigma-Aldrich Cat# MAK261 (Continued on next page) e1 Cell Host & Microbe 30, 1615–1629.e1–e5, November 9, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA sequencing data https://www.ncbi.nlm.nih.gov/geo/ GSE212421 Experimental models: Organisms/strains C57BL/6J mice The Jackson laboratory Cat# 000664 Oligonucleotides Primers for qRT-PCR used in this study are listed in Table S1 This paper N/A Software and algorithms Prism 6 GraphPad N/A QuantStudio real-time PCR software v1.3 Thermo Fisher N/A Image Lab 5.2.1 BioRad N/A iDEP.91 http://bioinformatics.sdstate.edu/idep/ N/A CLC Bio microbial genomics module https://www.qiagenbioinformatics.com/ plugins/clc-microbial-genomics-module/ N/A MetaboAnalyst 5.0 https://www.metaboanalyst.ca/ N/A Fiji Image J https://fiji.sc/ N/A Fusion 6.5v Omnitech Electronics Incorporated N/A MultiQuant 3.0.3.

    Purification:

    Article Title: N6-Methyladenosine Modification Controls Circular RNA Immunity.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-EspA-polyclonal, rabbit UT Southwestern, Vanessa Sperandio N/A Anti-EspB-polyclonal, rabbit UT Southwestern, Vanessa Sperandio N/A Anti-rabbit-HRP, goat BioRad Cat# 1706515; RRID: AB_2617112 Bacterial and virus strains E. coli HS Center for Vaccine Development at the University of Maryland School of Medicine N/A C. rodentium DBS100 Tufts University, John Leong N/A Chemicals, peptides, and recombinant proteins Dulbecco eagle medium, low glucose Invitrogen Cat# 11885-084 Cocaine Hydrochloride Sigma-Aldrich Cat#C5776 LB agar, Miller Fisher Scientific Cat# BP1425 LB broth, Miller Fisher Scientific Cat# BP9723 Ampicillin powder Sigma-Aldrich Cat# A9518 Naladixic acid power Fisher Scientific Cat# BP908 L-(+)-Arabinose Sigma-Aldrich Cat# A3256 Dextran sulfate sodium salt MP Biomedicals Cat#021601109 Phusion High fidelity PCR master mix Thermo Fisher Cat# F-531L Random primers Invitrogen Cat# 48190-011 Super script II reverse transcriptase Invitrogen Cat# 18064-014 Sybr green master mix Thermo Fisher Cat# 4309155 Sarcosine Sigma-Aldrich Cat#131776 Glycine Hydrochloride Sigma-Aldrich Cat# G2879 Trizol reagent Thermo Fisher Cat# 1559618 Chloroform IBM Scientific Cat# 67-66-3 dNTP mix 10mM Invitrogen Cat# 18-427-088 Bovine Serum Albumin Sigma-Aldrich Cat# A2153 Dopamine Hydrochloride Sigma-Aldrich Cat# H8502 6-hydroxydopamine Hydrochloride Sigma-Aldrich Cat# H4381 RNAlater-ICE Fisher Scientific Cat# 44-275-75 Protease inhibitor cocktail Sigma-Aldrich Cat# P8849-5 Dithiothreitol (DTT) Fisher Scientific Cat# BP172-25 Formaldehyde Fisher Scientific Cat# BP539 Brain heart infusion agar Becton Dickinson Cat# 2418102 Dextran sulfate sodium salt Fisher Scientific Cat# 02-160-1109 Metronidazole MP biomedicals Cat# 0215571025 Neomycin trisulfate salt hydrate Sigma-Aldrich Cat# N5285 Vancomycin hydrochloride Research Products International Cat# V06500 Critical Commercial Assays RNeasy Power Microbiome Qiagen Cat# 26000-50 RNeasy Plus Mini Kit Qiagen Cat# 74134 RiboPure RNA isolation Kit Ambion Cat# 1405072 GenElute Plasmid Miniprep Sigma Cat# PLN350-1KT QIAquick PCR purification kit Qiagen Cat# 28906 Glycine Assay Kit Sigma-Aldrich Cat# MAK261 (Continued on next page) e1 Cell Host & Microbe 30, 1615–1629.e1–e5, November 9, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA sequencing data https://www.ncbi.nlm.nih.gov/geo/ GSE212421 Experimental models: Organisms/strains C57BL/6J mice The Jackson laboratory Cat# 000664 Oligonucleotides Primers for qRT-PCR used in this study are listed in Table S1 This paper N/A Software and algorithms Prism 6 GraphPad N/A QuantStudio real-time PCR software v1.3 Thermo Fisher N/A Image Lab 5.2.1 BioRad N/A iDEP.91 http://bioinformatics.sdstate.edu/idep/ N/A CLC Bio microbial genomics module https://www.qiagenbioinformatics.com/ plugins/clc-microbial-genomics-module/ N/A MetaboAnalyst 5.0 https://www.metaboanalyst.ca/ N/A Fiji Image J https://fiji.sc/ N/A Fusion 6.5v Omnitech Electronics Incorporated N/A MultiQuant 3.0.3.



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