Review



gst nuak2 1 628  (Carna Inc)


Bioz Verified Symbol Carna Inc is a verified supplier
Bioz Manufacturer Symbol Carna Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Carna Inc gst nuak2 1 628
    Gst Nuak2 1 628, supplied by Carna Inc, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/02-127/NuaK2/pm37887106-120-0-4
    Average 94 stars, based on 2 article reviews
    gst nuak2 1 628 - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    In Vitro:

    Article Title: Extract2Chip-Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance.
    Article Snippet: synthesized and cloned into pRSF-DuetTM-1 in MCS 2, replacing the His6-TEV-CypD(43-207)-AviTag coding sequence, by GenScript (Piscataway, NJ, USA). .. GST-NUAK2(1-628) was purchased from Carna Biosciences, Inc (Kobe, Japan). .. Mutant 6 I62L, V116I, E139D, T204H, V205Q, L128I, L130I, N66T, F67Y, A68G, F199L,G137R, L72K # The mutations indicated in bold refer to the uniqueness

    Article Title: Extract2Chip—Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance
    Article Snippet: and cloned into pRSF-Duet TM -1 in MCS 2, replacing the His 6 -TEV-CypD(43-207)-AviTag coding sequence, by GenScript (Piscataway, NJ, USA). .. GST-NUAK2(1-628) was purchased from Carna Biosciences, Inc (Kobe, Japan).

    Article Title: PKA-Independent Vasopressin Signaling in Renal Collecting Duct
    Article Snippet: (ab204852, Abcam, Cambridge, MA) and SAMS-tide (ab120182) were used as standard peptides for SIK and AMPK phosphorylation. .. All SNF1-subfamily enzymes (AMPK, MARK3, QIK (SIK2), NUAK2, and MELK) were purchased from Carna Biosciences (Chuo-ku, Kobe, Japan). .. Recombinant AMPK (AMPKα1β1γ1, AMPKα2β2γ1, AMPKα2β1γ1) was custom-made without phosphorylation at T183 (in AMPKα1) or T172 (in AM

    Inhibition:

    Article Title: Extract2Chip-Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance.
    Article Snippet: synthesized and cloned into pRSF-DuetTM-1 in MCS 2, replacing the His6-TEV-CypD(43-207)-AviTag coding sequence, by GenScript (Piscataway, NJ, USA). .. GST-NUAK2(1-628) was purchased from Carna Biosciences, Inc (Kobe, Japan). .. Mutant 6 I62L, V116I, E139D, T204H, V205Q, L128I, L130I, N66T, F67Y, A68G, F199L,G137R, L72K # The mutations indicated in bold refer to the uniqueness

    Article Title: Extract2Chip—Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance
    Article Snippet: and cloned into pRSF-Duet TM -1 in MCS 2, replacing the His 6 -TEV-CypD(43-207)-AviTag coding sequence, by GenScript (Piscataway, NJ, USA). .. GST-NUAK2(1-628) was purchased from Carna Biosciences, Inc (Kobe, Japan).

    Article Title: PKA-Independent Vasopressin Signaling in Renal Collecting Duct
    Article Snippet: (ab204852, Abcam, Cambridge, MA) and SAMS-tide (ab120182) were used as standard peptides for SIK and AMPK phosphorylation. .. All SNF1-subfamily enzymes (AMPK, MARK3, QIK (SIK2), NUAK2, and MELK) were purchased from Carna Biosciences (Chuo-ku, Kobe, Japan). .. Recombinant AMPK (AMPKα1β1γ1, AMPKα2β2γ1, AMPKα2β1γ1) was custom-made without phosphorylation at T183 (in AMPKα1) or T172 (in AM

    Injection:

    Article Title: Extract2Chip-Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance.
    Article Snippet: synthesized and cloned into pRSF-DuetTM-1 in MCS 2, replacing the His6-TEV-CypD(43-207)-AviTag coding sequence, by GenScript (Piscataway, NJ, USA). .. GST-NUAK2(1-628) was purchased from Carna Biosciences, Inc (Kobe, Japan). .. Mutant 6 I62L, V116I, E139D, T204H, V205Q, L128I, L130I, N66T, F67Y, A68G, F199L,G137R, L72K # The mutations indicated in bold refer to the uniqueness

    Article Title: Extract2Chip—Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance
    Article Snippet: and cloned into pRSF-Duet TM -1 in MCS 2, replacing the His 6 -TEV-CypD(43-207)-AviTag coding sequence, by GenScript (Piscataway, NJ, USA). .. GST-NUAK2(1-628) was purchased from Carna Biosciences, Inc (Kobe, Japan).

    Article Title: PKA-Independent Vasopressin Signaling in Renal Collecting Duct
    Article Snippet: (ab204852, Abcam, Cambridge, MA) and SAMS-tide (ab120182) were used as standard peptides for SIK and AMPK phosphorylation. .. All SNF1-subfamily enzymes (AMPK, MARK3, QIK (SIK2), NUAK2, and MELK) were purchased from Carna Biosciences (Chuo-ku, Kobe, Japan). .. Recombinant AMPK (AMPKα1β1γ1, AMPKα2β2γ1, AMPKα2β1γ1) was custom-made without phosphorylation at T183 (in AMPKα1) or T172 (in AM

    SDS Page:

    Article Title: Extract2Chip-Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance.
    Article Snippet: synthesized and cloned into pRSF-DuetTM-1 in MCS 2, replacing the His6-TEV-CypD(43-207)-AviTag coding sequence, by GenScript (Piscataway, NJ, USA). .. GST-NUAK2(1-628) was purchased from Carna Biosciences, Inc (Kobe, Japan). .. Mutant 6 I62L, V116I, E139D, T204H, V205Q, L128I, L130I, N66T, F67Y, A68G, F199L,G137R, L72K # The mutations indicated in bold refer to the uniqueness

    Article Title: Extract2Chip—Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance
    Article Snippet: and cloned into pRSF-Duet TM -1 in MCS 2, replacing the His 6 -TEV-CypD(43-207)-AviTag coding sequence, by GenScript (Piscataway, NJ, USA). .. GST-NUAK2(1-628) was purchased from Carna Biosciences, Inc (Kobe, Japan).

    Article Title: PKA-Independent Vasopressin Signaling in Renal Collecting Duct
    Article Snippet: (ab204852, Abcam, Cambridge, MA) and SAMS-tide (ab120182) were used as standard peptides for SIK and AMPK phosphorylation. .. All SNF1-subfamily enzymes (AMPK, MARK3, QIK (SIK2), NUAK2, and MELK) were purchased from Carna Biosciences (Chuo-ku, Kobe, Japan). .. Recombinant AMPK (AMPKα1β1γ1, AMPKα2β2γ1, AMPKα2β1γ1) was custom-made without phosphorylation at T183 (in AMPKα1) or T172 (in AM

    Marker:

    Article Title: Extract2Chip-Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance.
    Article Snippet: synthesized and cloned into pRSF-DuetTM-1 in MCS 2, replacing the His6-TEV-CypD(43-207)-AviTag coding sequence, by GenScript (Piscataway, NJ, USA). .. GST-NUAK2(1-628) was purchased from Carna Biosciences, Inc (Kobe, Japan). .. Mutant 6 I62L, V116I, E139D, T204H, V205Q, L128I, L130I, N66T, F67Y, A68G, F199L,G137R, L72K # The mutations indicated in bold refer to the uniqueness

    Article Title: Extract2Chip—Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance
    Article Snippet: and cloned into pRSF-Duet TM -1 in MCS 2, replacing the His 6 -TEV-CypD(43-207)-AviTag coding sequence, by GenScript (Piscataway, NJ, USA). .. GST-NUAK2(1-628) was purchased from Carna Biosciences, Inc (Kobe, Japan).

    Article Title: PKA-Independent Vasopressin Signaling in Renal Collecting Duct
    Article Snippet: (ab204852, Abcam, Cambridge, MA) and SAMS-tide (ab120182) were used as standard peptides for SIK and AMPK phosphorylation. .. All SNF1-subfamily enzymes (AMPK, MARK3, QIK (SIK2), NUAK2, and MELK) were purchased from Carna Biosciences (Chuo-ku, Kobe, Japan). .. Recombinant AMPK (AMPKα1β1γ1, AMPKα2β2γ1, AMPKα2β1γ1) was custom-made without phosphorylation at T183 (in AMPKα1) or T172 (in AM

    Concentration Assay:

    Article Title: Extract2Chip-Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance.
    Article Snippet: synthesized and cloned into pRSF-DuetTM-1 in MCS 2, replacing the His6-TEV-CypD(43-207)-AviTag coding sequence, by GenScript (Piscataway, NJ, USA). .. GST-NUAK2(1-628) was purchased from Carna Biosciences, Inc (Kobe, Japan). .. Mutant 6 I62L, V116I, E139D, T204H, V205Q, L128I, L130I, N66T, F67Y, A68G, F199L,G137R, L72K # The mutations indicated in bold refer to the uniqueness

    Article Title: Extract2Chip—Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance
    Article Snippet: and cloned into pRSF-Duet TM -1 in MCS 2, replacing the His 6 -TEV-CypD(43-207)-AviTag coding sequence, by GenScript (Piscataway, NJ, USA). .. GST-NUAK2(1-628) was purchased from Carna Biosciences, Inc (Kobe, Japan).

    Article Title: PKA-Independent Vasopressin Signaling in Renal Collecting Duct
    Article Snippet: (ab204852, Abcam, Cambridge, MA) and SAMS-tide (ab120182) were used as standard peptides for SIK and AMPK phosphorylation. .. All SNF1-subfamily enzymes (AMPK, MARK3, QIK (SIK2), NUAK2, and MELK) were purchased from Carna Biosciences (Chuo-ku, Kobe, Japan). .. Recombinant AMPK (AMPKα1β1γ1, AMPKα2β2γ1, AMPKα2β1γ1) was custom-made without phosphorylation at T183 (in AMPKα1) or T172 (in AM

    Western Blot:

    Article Title: Extract2Chip-Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance.
    Article Snippet: synthesized and cloned into pRSF-DuetTM-1 in MCS 2, replacing the His6-TEV-CypD(43-207)-AviTag coding sequence, by GenScript (Piscataway, NJ, USA). .. GST-NUAK2(1-628) was purchased from Carna Biosciences, Inc (Kobe, Japan). .. Mutant 6 I62L, V116I, E139D, T204H, V205Q, L128I, L130I, N66T, F67Y, A68G, F199L,G137R, L72K # The mutations indicated in bold refer to the uniqueness

    Article Title: Extract2Chip—Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance
    Article Snippet: and cloned into pRSF-Duet TM -1 in MCS 2, replacing the His 6 -TEV-CypD(43-207)-AviTag coding sequence, by GenScript (Piscataway, NJ, USA). .. GST-NUAK2(1-628) was purchased from Carna Biosciences, Inc (Kobe, Japan).

    Article Title: PKA-Independent Vasopressin Signaling in Renal Collecting Duct
    Article Snippet: (ab204852, Abcam, Cambridge, MA) and SAMS-tide (ab120182) were used as standard peptides for SIK and AMPK phosphorylation. .. All SNF1-subfamily enzymes (AMPK, MARK3, QIK (SIK2), NUAK2, and MELK) were purchased from Carna Biosciences (Chuo-ku, Kobe, Japan). .. Recombinant AMPK (AMPKα1β1γ1, AMPKα2β2γ1, AMPKα2β1γ1) was custom-made without phosphorylation at T183 (in AMPKα1) or T172 (in AM

    Expressing:

    Article Title: Extract2Chip-Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance.
    Article Snippet: synthesized and cloned into pRSF-DuetTM-1 in MCS 2, replacing the His6-TEV-CypD(43-207)-AviTag coding sequence, by GenScript (Piscataway, NJ, USA). .. GST-NUAK2(1-628) was purchased from Carna Biosciences, Inc (Kobe, Japan). .. Mutant 6 I62L, V116I, E139D, T204H, V205Q, L128I, L130I, N66T, F67Y, A68G, F199L,G137R, L72K # The mutations indicated in bold refer to the uniqueness

    Article Title: Extract2Chip—Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance
    Article Snippet: and cloned into pRSF-Duet TM -1 in MCS 2, replacing the His 6 -TEV-CypD(43-207)-AviTag coding sequence, by GenScript (Piscataway, NJ, USA). .. GST-NUAK2(1-628) was purchased from Carna Biosciences, Inc (Kobe, Japan).

    Article Title: PKA-Independent Vasopressin Signaling in Renal Collecting Duct
    Article Snippet: (ab204852, Abcam, Cambridge, MA) and SAMS-tide (ab120182) were used as standard peptides for SIK and AMPK phosphorylation. .. All SNF1-subfamily enzymes (AMPK, MARK3, QIK (SIK2), NUAK2, and MELK) were purchased from Carna Biosciences (Chuo-ku, Kobe, Japan). .. Recombinant AMPK (AMPKα1β1γ1, AMPKα2β2γ1, AMPKα2β1γ1) was custom-made without phosphorylation at T183 (in AMPKα1) or T172 (in AM

    Recombinant:

    Article Title: Extract2Chip-Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance.
    Article Snippet: synthesized and cloned into pRSF-DuetTM-1 in MCS 2, replacing the His6-TEV-CypD(43-207)-AviTag coding sequence, by GenScript (Piscataway, NJ, USA). .. GST-NUAK2(1-628) was purchased from Carna Biosciences, Inc (Kobe, Japan). .. Mutant 6 I62L, V116I, E139D, T204H, V205Q, L128I, L130I, N66T, F67Y, A68G, F199L,G137R, L72K # The mutations indicated in bold refer to the uniqueness

    Article Title: Extract2Chip—Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance
    Article Snippet: and cloned into pRSF-Duet TM -1 in MCS 2, replacing the His 6 -TEV-CypD(43-207)-AviTag coding sequence, by GenScript (Piscataway, NJ, USA). .. GST-NUAK2(1-628) was purchased from Carna Biosciences, Inc (Kobe, Japan).

    Article Title: PKA-Independent Vasopressin Signaling in Renal Collecting Duct
    Article Snippet: (ab204852, Abcam, Cambridge, MA) and SAMS-tide (ab120182) were used as standard peptides for SIK and AMPK phosphorylation. .. All SNF1-subfamily enzymes (AMPK, MARK3, QIK (SIK2), NUAK2, and MELK) were purchased from Carna Biosciences (Chuo-ku, Kobe, Japan). .. Recombinant AMPK (AMPKα1β1γ1, AMPKα2β2γ1, AMPKα2β1γ1) was custom-made without phosphorylation at T183 (in AMPKα1) or T172 (in AM

    Incubation:

    Article Title: Extract2Chip-Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance.
    Article Snippet: synthesized and cloned into pRSF-DuetTM-1 in MCS 2, replacing the His6-TEV-CypD(43-207)-AviTag coding sequence, by GenScript (Piscataway, NJ, USA). .. GST-NUAK2(1-628) was purchased from Carna Biosciences, Inc (Kobe, Japan). .. Mutant 6 I62L, V116I, E139D, T204H, V205Q, L128I, L130I, N66T, F67Y, A68G, F199L,G137R, L72K # The mutations indicated in bold refer to the uniqueness

    Article Title: Extract2Chip—Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance
    Article Snippet: and cloned into pRSF-Duet TM -1 in MCS 2, replacing the His 6 -TEV-CypD(43-207)-AviTag coding sequence, by GenScript (Piscataway, NJ, USA). .. GST-NUAK2(1-628) was purchased from Carna Biosciences, Inc (Kobe, Japan).

    Article Title: PKA-Independent Vasopressin Signaling in Renal Collecting Duct
    Article Snippet: (ab204852, Abcam, Cambridge, MA) and SAMS-tide (ab120182) were used as standard peptides for SIK and AMPK phosphorylation. .. All SNF1-subfamily enzymes (AMPK, MARK3, QIK (SIK2), NUAK2, and MELK) were purchased from Carna Biosciences (Chuo-ku, Kobe, Japan). .. Recombinant AMPK (AMPKα1β1γ1, AMPKα2β2γ1, AMPKα2β1γ1) was custom-made without phosphorylation at T183 (in AMPKα1) or T172 (in AM

    Phospho-proteomics:

    Article Title: Extract2Chip-Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance.
    Article Snippet: synthesized and cloned into pRSF-DuetTM-1 in MCS 2, replacing the His6-TEV-CypD(43-207)-AviTag coding sequence, by GenScript (Piscataway, NJ, USA). .. GST-NUAK2(1-628) was purchased from Carna Biosciences, Inc (Kobe, Japan). .. Mutant 6 I62L, V116I, E139D, T204H, V205Q, L128I, L130I, N66T, F67Y, A68G, F199L,G137R, L72K # The mutations indicated in bold refer to the uniqueness

    Article Title: Extract2Chip—Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance
    Article Snippet: and cloned into pRSF-Duet TM -1 in MCS 2, replacing the His 6 -TEV-CypD(43-207)-AviTag coding sequence, by GenScript (Piscataway, NJ, USA). .. GST-NUAK2(1-628) was purchased from Carna Biosciences, Inc (Kobe, Japan).

    Article Title: PKA-Independent Vasopressin Signaling in Renal Collecting Duct
    Article Snippet: (ab204852, Abcam, Cambridge, MA) and SAMS-tide (ab120182) were used as standard peptides for SIK and AMPK phosphorylation. .. All SNF1-subfamily enzymes (AMPK, MARK3, QIK (SIK2), NUAK2, and MELK) were purchased from Carna Biosciences (Chuo-ku, Kobe, Japan). .. Recombinant AMPK (AMPKα1β1γ1, AMPKα2β2γ1, AMPKα2β1γ1) was custom-made without phosphorylation at T183 (in AMPKα1) or T172 (in AM

    Control:

    Article Title: Extract2Chip-Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance.
    Article Snippet: synthesized and cloned into pRSF-DuetTM-1 in MCS 2, replacing the His6-TEV-CypD(43-207)-AviTag coding sequence, by GenScript (Piscataway, NJ, USA). .. GST-NUAK2(1-628) was purchased from Carna Biosciences, Inc (Kobe, Japan). .. Mutant 6 I62L, V116I, E139D, T204H, V205Q, L128I, L130I, N66T, F67Y, A68G, F199L,G137R, L72K # The mutations indicated in bold refer to the uniqueness

    Article Title: Extract2Chip—Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance
    Article Snippet: and cloned into pRSF-Duet TM -1 in MCS 2, replacing the His 6 -TEV-CypD(43-207)-AviTag coding sequence, by GenScript (Piscataway, NJ, USA). .. GST-NUAK2(1-628) was purchased from Carna Biosciences, Inc (Kobe, Japan).

    Article Title: PKA-Independent Vasopressin Signaling in Renal Collecting Duct
    Article Snippet: (ab204852, Abcam, Cambridge, MA) and SAMS-tide (ab120182) were used as standard peptides for SIK and AMPK phosphorylation. .. All SNF1-subfamily enzymes (AMPK, MARK3, QIK (SIK2), NUAK2, and MELK) were purchased from Carna Biosciences (Chuo-ku, Kobe, Japan). .. Recombinant AMPK (AMPKα1β1γ1, AMPKα2β2γ1, AMPKα2β1γ1) was custom-made without phosphorylation at T183 (in AMPKα1) or T172 (in AM



    Similar Products

    mut express ii fast mutagenesis kit v2 127  (Vazyme Biotech Co)
    97
    Vazyme Biotech Co mut express ii fast mutagenesis kit v2 127
    Mut Express Ii Fast Mutagenesis Kit V2 127, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/02-127/Mut+Express+II+Fast+Mutagenesis+Kit+V2/pm41832996-42-91-90
    Average 97 stars, based on 1 article reviews
    mut express ii fast mutagenesis kit v2 127 - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier
    annexin v pi 127 apoptosis detection kit  (Vazyme Biotech Co)
    97
    Vazyme Biotech Co annexin v pi 127 apoptosis detection kit
    Annexin V Pi 127 Apoptosis Detection Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/02-127/Annexin+V-FITC+PI+Apoptosis+Detection+Kit/pm40484336-65-7-14
    Average 97 stars, based on 1 article reviews
    annexin v pi 127 apoptosis detection kit - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier
    flow cytometry based macs marker screen  (Miltenyi Biotec)
    97
    Miltenyi Biotec flow cytometry based macs marker screen
    Identification and validation of cell surface sorting markers for iPSC-derived P1 RPE cells (A) P1 RPE cells were screened for expression of 371 antibodies directed against cell surface markers using flow <t>cytometry.</t> Hits were identified based on correlation to side scatter (SSC) intensity as proxy for pigmentation, here shown for CD54 (ICAM-1) (positive correlation) and PSA-NCAM (negative correlation). (B) Combinatorial staining for CD54 and PSA-NCAM reliably discriminates SSC-low non-target (unpigmented, backgated in blue) and SSC-high target cells (pigmented, backgated in orange). (C) Immunofluorescent staining of RPE cells in culture shows cells expressing PSA-NCAM but little CD54 mostly in regions lacking pigmentation and proper cobblestone morphology (areas highlighted by dashed lines). Co-expression of CD54 and PSA-NCAM appears to occur primarily in regions of intermediate pigmentation levels and mixed cobblestone morphology (area highlighted by solid line) (D) Flow scatter and corresponding transmitted light microscopy images for unsorted (UNS), CD54 + /PSA-NCAM - -enriched (ENR) and CD54 + /PSA-NCAM - -reduced (RED) cell fractions. Cells were sorted using the MACSQuant Tyto cell sorter to maximize target cell purity. Replated fractions reveal that pigmented cells can be efficiently enriched with the dual marker strategy. (E) CD54 + /PSA-NCAM - sorting of frozen and freshly harvested cells consistently yielded a target fraction of high purity. Circles show single re-analysis measurements, solid points their average. Scale bars: 100 µm (D), 50 µm (C).
    Flow Cytometry Based Macs Marker Screen, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/02-127/MACS+Marker+Screen%2C+anti-human%2C+version+02/bio_rxiv__2024__10__15__618147-222-21-29
    Average 97 stars, based on 1 article reviews
    flow cytometry based macs marker screen - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier
    gst nuak2 1 628  (Carna Inc)
    94
    Carna Inc gst nuak2 1 628
    Identification and validation of cell surface sorting markers for iPSC-derived P1 RPE cells (A) P1 RPE cells were screened for expression of 371 antibodies directed against cell surface markers using flow <t>cytometry.</t> Hits were identified based on correlation to side scatter (SSC) intensity as proxy for pigmentation, here shown for CD54 (ICAM-1) (positive correlation) and PSA-NCAM (negative correlation). (B) Combinatorial staining for CD54 and PSA-NCAM reliably discriminates SSC-low non-target (unpigmented, backgated in blue) and SSC-high target cells (pigmented, backgated in orange). (C) Immunofluorescent staining of RPE cells in culture shows cells expressing PSA-NCAM but little CD54 mostly in regions lacking pigmentation and proper cobblestone morphology (areas highlighted by dashed lines). Co-expression of CD54 and PSA-NCAM appears to occur primarily in regions of intermediate pigmentation levels and mixed cobblestone morphology (area highlighted by solid line) (D) Flow scatter and corresponding transmitted light microscopy images for unsorted (UNS), CD54 + /PSA-NCAM - -enriched (ENR) and CD54 + /PSA-NCAM - -reduced (RED) cell fractions. Cells were sorted using the MACSQuant Tyto cell sorter to maximize target cell purity. Replated fractions reveal that pigmented cells can be efficiently enriched with the dual marker strategy. (E) CD54 + /PSA-NCAM - sorting of frozen and freshly harvested cells consistently yielded a target fraction of high purity. Circles show single re-analysis measurements, solid points their average. Scale bars: 100 µm (D), 50 µm (C).
    Gst Nuak2 1 628, supplied by Carna Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/02-127/NuaK2/pm37887106-120-0-4
    Average 94 stars, based on 1 article reviews
    gst nuak2 1 628 - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    plates  (Miltenyi Biotec)
    97
    Miltenyi Biotec plates
    Identification and validation of cell surface sorting markers for iPSC-derived P1 RPE cells (A) P1 RPE cells were screened for expression of 371 antibodies directed against cell surface markers using flow <t>cytometry.</t> Hits were identified based on correlation to side scatter (SSC) intensity as proxy for pigmentation, here shown for CD54 (ICAM-1) (positive correlation) and PSA-NCAM (negative correlation). (B) Combinatorial staining for CD54 and PSA-NCAM reliably discriminates SSC-low non-target (unpigmented, backgated in blue) and SSC-high target cells (pigmented, backgated in orange). (C) Immunofluorescent staining of RPE cells in culture shows cells expressing PSA-NCAM but little CD54 mostly in regions lacking pigmentation and proper cobblestone morphology (areas highlighted by dashed lines). Co-expression of CD54 and PSA-NCAM appears to occur primarily in regions of intermediate pigmentation levels and mixed cobblestone morphology (area highlighted by solid line) (D) Flow scatter and corresponding transmitted light microscopy images for unsorted (UNS), CD54 + /PSA-NCAM - -enriched (ENR) and CD54 + /PSA-NCAM - -reduced (RED) cell fractions. Cells were sorted using the MACSQuant Tyto cell sorter to maximize target cell purity. Replated fractions reveal that pigmented cells can be efficiently enriched with the dual marker strategy. (E) CD54 + /PSA-NCAM - sorting of frozen and freshly harvested cells consistently yielded a target fraction of high purity. Circles show single re-analysis measurements, solid points their average. Scale bars: 100 µm (D), 50 µm (C).
    Plates, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/02-127/MACS+Marker+Screen%2C+anti-human%2C+version+02/pm36965292-68-10-17
    Average 97 stars, based on 1 article reviews
    plates - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier
    human version 02  (Miltenyi Biotec)
    97
    Miltenyi Biotec human version 02
    Identification and validation of cell surface sorting markers for iPSC-derived P1 RPE cells (A) P1 RPE cells were screened for expression of 371 antibodies directed against cell surface markers using flow <t>cytometry.</t> Hits were identified based on correlation to side scatter (SSC) intensity as proxy for pigmentation, here shown for CD54 (ICAM-1) (positive correlation) and PSA-NCAM (negative correlation). (B) Combinatorial staining for CD54 and PSA-NCAM reliably discriminates SSC-low non-target (unpigmented, backgated in blue) and SSC-high target cells (pigmented, backgated in orange). (C) Immunofluorescent staining of RPE cells in culture shows cells expressing PSA-NCAM but little CD54 mostly in regions lacking pigmentation and proper cobblestone morphology (areas highlighted by dashed lines). Co-expression of CD54 and PSA-NCAM appears to occur primarily in regions of intermediate pigmentation levels and mixed cobblestone morphology (area highlighted by solid line) (D) Flow scatter and corresponding transmitted light microscopy images for unsorted (UNS), CD54 + /PSA-NCAM - -enriched (ENR) and CD54 + /PSA-NCAM - -reduced (RED) cell fractions. Cells were sorted using the MACSQuant Tyto cell sorter to maximize target cell purity. Replated fractions reveal that pigmented cells can be efficiently enriched with the dual marker strategy. (E) CD54 + /PSA-NCAM - sorting of frozen and freshly harvested cells consistently yielded a target fraction of high purity. Circles show single re-analysis measurements, solid points their average. Scale bars: 100 µm (D), 50 µm (C).
    Human Version 02, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/02-127/MACS+Marker+Screen%2C+anti-human%2C+version+02/pm36965292-50-3-6
    Average 97 stars, based on 1 article reviews
    human version 02 - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier
    macs marker screen  (Miltenyi Biotec)
    97
    Miltenyi Biotec macs marker screen
    Identification and validation of cell surface sorting markers for iPSC-derived P1 RPE cells (A) P1 RPE cells were screened for expression of 371 antibodies directed against cell surface markers using flow <t>cytometry.</t> Hits were identified based on correlation to side scatter (SSC) intensity as proxy for pigmentation, here shown for CD54 (ICAM-1) (positive correlation) and PSA-NCAM (negative correlation). (B) Combinatorial staining for CD54 and PSA-NCAM reliably discriminates SSC-low non-target (unpigmented, backgated in blue) and SSC-high target cells (pigmented, backgated in orange). (C) Immunofluorescent staining of RPE cells in culture shows cells expressing PSA-NCAM but little CD54 mostly in regions lacking pigmentation and proper cobblestone morphology (areas highlighted by dashed lines). Co-expression of CD54 and PSA-NCAM appears to occur primarily in regions of intermediate pigmentation levels and mixed cobblestone morphology (area highlighted by solid line) (D) Flow scatter and corresponding transmitted light microscopy images for unsorted (UNS), CD54 + /PSA-NCAM - -enriched (ENR) and CD54 + /PSA-NCAM - -reduced (RED) cell fractions. Cells were sorted using the MACSQuant Tyto cell sorter to maximize target cell purity. Replated fractions reveal that pigmented cells can be efficiently enriched with the dual marker strategy. (E) CD54 + /PSA-NCAM - sorting of frozen and freshly harvested cells consistently yielded a target fraction of high purity. Circles show single re-analysis measurements, solid points their average. Scale bars: 100 µm (D), 50 µm (C).
    Macs Marker Screen, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/02-127/MACS+Marker+Screen%2C+anti-human%2C+version+02/pm35758160-378-9-17
    Average 97 stars, based on 1 article reviews
    macs marker screen - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    Identification and validation of cell surface sorting markers for iPSC-derived P1 RPE cells (A) P1 RPE cells were screened for expression of 371 antibodies directed against cell surface markers using flow cytometry. Hits were identified based on correlation to side scatter (SSC) intensity as proxy for pigmentation, here shown for CD54 (ICAM-1) (positive correlation) and PSA-NCAM (negative correlation). (B) Combinatorial staining for CD54 and PSA-NCAM reliably discriminates SSC-low non-target (unpigmented, backgated in blue) and SSC-high target cells (pigmented, backgated in orange). (C) Immunofluorescent staining of RPE cells in culture shows cells expressing PSA-NCAM but little CD54 mostly in regions lacking pigmentation and proper cobblestone morphology (areas highlighted by dashed lines). Co-expression of CD54 and PSA-NCAM appears to occur primarily in regions of intermediate pigmentation levels and mixed cobblestone morphology (area highlighted by solid line) (D) Flow scatter and corresponding transmitted light microscopy images for unsorted (UNS), CD54 + /PSA-NCAM - -enriched (ENR) and CD54 + /PSA-NCAM - -reduced (RED) cell fractions. Cells were sorted using the MACSQuant Tyto cell sorter to maximize target cell purity. Replated fractions reveal that pigmented cells can be efficiently enriched with the dual marker strategy. (E) CD54 + /PSA-NCAM - sorting of frozen and freshly harvested cells consistently yielded a target fraction of high purity. Circles show single re-analysis measurements, solid points their average. Scale bars: 100 µm (D), 50 µm (C).

    Journal: bioRxiv

    Article Title: Extensive monolayer formation depends on a subpopulation of transplanted human iPSC-derived RPE cells

    doi: 10.1101/2024.10.15.618147

    Figure Lengend Snippet: Identification and validation of cell surface sorting markers for iPSC-derived P1 RPE cells (A) P1 RPE cells were screened for expression of 371 antibodies directed against cell surface markers using flow cytometry. Hits were identified based on correlation to side scatter (SSC) intensity as proxy for pigmentation, here shown for CD54 (ICAM-1) (positive correlation) and PSA-NCAM (negative correlation). (B) Combinatorial staining for CD54 and PSA-NCAM reliably discriminates SSC-low non-target (unpigmented, backgated in blue) and SSC-high target cells (pigmented, backgated in orange). (C) Immunofluorescent staining of RPE cells in culture shows cells expressing PSA-NCAM but little CD54 mostly in regions lacking pigmentation and proper cobblestone morphology (areas highlighted by dashed lines). Co-expression of CD54 and PSA-NCAM appears to occur primarily in regions of intermediate pigmentation levels and mixed cobblestone morphology (area highlighted by solid line) (D) Flow scatter and corresponding transmitted light microscopy images for unsorted (UNS), CD54 + /PSA-NCAM - -enriched (ENR) and CD54 + /PSA-NCAM - -reduced (RED) cell fractions. Cells were sorted using the MACSQuant Tyto cell sorter to maximize target cell purity. Replated fractions reveal that pigmented cells can be efficiently enriched with the dual marker strategy. (E) CD54 + /PSA-NCAM - sorting of frozen and freshly harvested cells consistently yielded a target fraction of high purity. Circles show single re-analysis measurements, solid points their average. Scale bars: 100 µm (D), 50 µm (C).

    Article Snippet: P1 RPE cells derived from CRTD1 iPSC were screened for expression of 371 antibodies directed against cell surface markers using the flow cytometry-based MACS® Marker Screen, anti-human, version 01 (Miltenyi Biotec, 130-110-055) according to the manufacturer’s instructions.

    Techniques: Biomarker Discovery, Derivative Assay, Expressing, Flow Cytometry, Staining, Light Microscopy, Marker

    CD54 + /PSA-NCAM - RPE cells show increased monolayer formation after transplantation. (A – C) Flat-mounted eyecups that received P1 RPE single cell suspensions which were either unsorted (UNS; A), enriched (ENR, B), or reduced (RED, C) in CD54 + /PSA-NCAM - cells show large areas of donor cells identified by Human Nuclei staining (graft region outlined with dotted lines). While in UNS (A, A’) and RED (C) samples the proportion of area with cell clusters (visible through intense Human Nuclei staining) is high, in ENR transplanted retinas the proportion of monolayer with typical cobblestone morphology as seen by staining for F-actin is high (B, B’, B’’). Human nuclei visibility in properly structured monolayers is concealed due to high pigmentation of donor cells (B’’; for single z-planes see figures S4E and F), but better visible at monolayer edges (B’). Magnification of an RPE-depleted area without donor cells is shown in C’. (D) Outlines of monolayer cells from B’’ as detected with the REShAPE tool( Ortolan et al , 2022 ) show the possibility to analyse the grafts in flatmount view. (E-G) REShAPE analysis of the flatmounts A-C (corresponding REShAPE cell outline maps see figure S4G-I). (E) reveals a highly increased number of monolayer cells in the ENR graft in comparison to UNS or RED (UNS: 7453, ENR: 37607, RED: 6921). ENR monolayer cells on average appear larger than UNS or RED monolayer cells (F; mean areas: UNS 57 µm 2 , ENR 79 µm 2 , RED 55 µm 2 , violin plots of all cells detected with overlaid box-plots showing median and 25% quartiles, black lines showing the mean). In all three conditions human monolayer cells have mostly 5 or 6 neighboring cells, with the ENR graft having the highest ratio of cells with 5-7 neighbors (G; UNS: 67%, ENR: 82%, RED: 69%). (H) Monolayers formed in all conditions show a polarized morphology with basal collagen IV (COL4) and apical ATPase (ATP1A1) expression. Human nuclei are located basally, while pigmentation (dark in DIC images) appears mainly apically. Large, highly pigmented, round structures are frequently detected in clusters (see also A’) and sometimes in monolayer areas, likely representing pigment-filled macrophages (compare also figure S5A). (I, J) Quantification of monolayer proportion after transplantation of RPE cells from two separate differentiation rounds. While in both rounds the CD54 + /PSA-NCAM - ENR donor cell fractions robustly generated similarly high proportions of monolayer (ENR - I : 82.5% ± 3.5%; n=7; ENR – J: 69.5% ± 6.9%; n=4), the amount of monolayer generated by UNS (UNS - I: 67.3% ± 3.5%; n=5; UNS - J: 23.1% ± 3.3%, n=7) and RED cell fractions (RED - I: 55.2% ± 8.1%; n=6; RED - J: 6.9% ± 1.3%, n=9; Kruskal-Wallis with pairwise Wilcoxon test) strongly varied, correlating with different amounts of CD54 + /PSA-NCAM - cell purity within the different fractions (see also figure S4A for purity data related to and figure S4B for purity data related to ). (K) Quantification of monolayer proportion after transplantation of RPE cells from a third differentiation round, sorted with conventional flow cytometry (UNS: 17.9% ± 3.8%, n = 5; ENR: 55.3% ± 12.2%, n = 4; RED: 21.5% ± 7.2%, n = 4; Kruskal-Wallis with pairwise Wilcoxon test). Scale bars, 500 µm (A, B, C), 50 µm (A’, B’, B’’, C’, D) and 20 µm (H). Data are represented as mean ± SEM.

    Journal: bioRxiv

    Article Title: Extensive monolayer formation depends on a subpopulation of transplanted human iPSC-derived RPE cells

    doi: 10.1101/2024.10.15.618147

    Figure Lengend Snippet: CD54 + /PSA-NCAM - RPE cells show increased monolayer formation after transplantation. (A – C) Flat-mounted eyecups that received P1 RPE single cell suspensions which were either unsorted (UNS; A), enriched (ENR, B), or reduced (RED, C) in CD54 + /PSA-NCAM - cells show large areas of donor cells identified by Human Nuclei staining (graft region outlined with dotted lines). While in UNS (A, A’) and RED (C) samples the proportion of area with cell clusters (visible through intense Human Nuclei staining) is high, in ENR transplanted retinas the proportion of monolayer with typical cobblestone morphology as seen by staining for F-actin is high (B, B’, B’’). Human nuclei visibility in properly structured monolayers is concealed due to high pigmentation of donor cells (B’’; for single z-planes see figures S4E and F), but better visible at monolayer edges (B’). Magnification of an RPE-depleted area without donor cells is shown in C’. (D) Outlines of monolayer cells from B’’ as detected with the REShAPE tool( Ortolan et al , 2022 ) show the possibility to analyse the grafts in flatmount view. (E-G) REShAPE analysis of the flatmounts A-C (corresponding REShAPE cell outline maps see figure S4G-I). (E) reveals a highly increased number of monolayer cells in the ENR graft in comparison to UNS or RED (UNS: 7453, ENR: 37607, RED: 6921). ENR monolayer cells on average appear larger than UNS or RED monolayer cells (F; mean areas: UNS 57 µm 2 , ENR 79 µm 2 , RED 55 µm 2 , violin plots of all cells detected with overlaid box-plots showing median and 25% quartiles, black lines showing the mean). In all three conditions human monolayer cells have mostly 5 or 6 neighboring cells, with the ENR graft having the highest ratio of cells with 5-7 neighbors (G; UNS: 67%, ENR: 82%, RED: 69%). (H) Monolayers formed in all conditions show a polarized morphology with basal collagen IV (COL4) and apical ATPase (ATP1A1) expression. Human nuclei are located basally, while pigmentation (dark in DIC images) appears mainly apically. Large, highly pigmented, round structures are frequently detected in clusters (see also A’) and sometimes in monolayer areas, likely representing pigment-filled macrophages (compare also figure S5A). (I, J) Quantification of monolayer proportion after transplantation of RPE cells from two separate differentiation rounds. While in both rounds the CD54 + /PSA-NCAM - ENR donor cell fractions robustly generated similarly high proportions of monolayer (ENR - I : 82.5% ± 3.5%; n=7; ENR – J: 69.5% ± 6.9%; n=4), the amount of monolayer generated by UNS (UNS - I: 67.3% ± 3.5%; n=5; UNS - J: 23.1% ± 3.3%, n=7) and RED cell fractions (RED - I: 55.2% ± 8.1%; n=6; RED - J: 6.9% ± 1.3%, n=9; Kruskal-Wallis with pairwise Wilcoxon test) strongly varied, correlating with different amounts of CD54 + /PSA-NCAM - cell purity within the different fractions (see also figure S4A for purity data related to and figure S4B for purity data related to ). (K) Quantification of monolayer proportion after transplantation of RPE cells from a third differentiation round, sorted with conventional flow cytometry (UNS: 17.9% ± 3.8%, n = 5; ENR: 55.3% ± 12.2%, n = 4; RED: 21.5% ± 7.2%, n = 4; Kruskal-Wallis with pairwise Wilcoxon test). Scale bars, 500 µm (A, B, C), 50 µm (A’, B’, B’’, C’, D) and 20 µm (H). Data are represented as mean ± SEM.

    Article Snippet: P1 RPE cells derived from CRTD1 iPSC were screened for expression of 371 antibodies directed against cell surface markers using the flow cytometry-based MACS® Marker Screen, anti-human, version 01 (Miltenyi Biotec, 130-110-055) according to the manufacturer’s instructions.

    Techniques: Transplantation Assay, Staining, Comparison, Expressing, Generated, Flow Cytometry