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dithio bis  (Chem Impex International)


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    Chem Impex International dithio bis
    Dithio Bis, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/01899/5%2C5'-Dithio-bis/pmc06368189-100-26-13
    Average 95 stars, based on 2 article reviews
    dithio bis - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    other:

    Article Title: Bioavailable Sulforaphane Quantitation in Plasma by LC-MS/MS Is Enhanced by Blocking Thiols.
    Article Snippet: 5,5′- dithio-bis (2-nitrobenzoic acid) (DTNB, Ellman’s Reagent) was at 99.9% purity and purchased from Chem-Impex International (Wood Dale, IL, USA).

    Article Title: Bioavailable Sulforaphane Quantitation in Plasma by LC–MS/MS Is Enhanced by Blocking Thiols
    Article Snippet: 5,5′-dithio-bis (2-nitrobenzoic acid) (DTNB, Ellman’s Reagent) was at 99.9% purity and purchased from Chem-Impex International (Wood Dale, IL, USA).

    High Performance Liquid Chromatography:

    Article Title: Orthogonal click reactions enable the synthesis of ECM-mimetic PEG hydrogels without multi-arm precursors
    Article Snippet: Materials PEG di-maleimide (5,000 g/mol) and PEG di-thiol (3,400 g/mol) were purchased from Laysan Bio Inc. Rink amide MBHA resin (100–200 mesh) was purchased from Novabiochem. .. Fmoc protected amino acids, 2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexauorophosphate (HBTU), and N-methyl-pyroliddinone (NMP) were purchased from Chem-Impex International Inc. Triethanolamine (TEOA), diisopropyl ethylamine (DIEA), deuterium oxide (D 2 O), 5,5’-dithio-bis-(2-nitrobenzoic acid) (DTNB or Ellman’s reagent), phenol, piperidine, triisopropylsilane (TIS), trifluoroacetic acid (TFA) reagent grade and HPLC grade were purchased from Sigma Aldrich. .. L-cysteine, 5-norbornene 2-carboxcylic acid was purchased from Alfa Aesar.

    Modification:

    Article Title: Measuring interactions of DNA with nanoporous protein crystals by atomic force microscopy.
    Article Snippet: Crosslinked porous protein crystals are a new biomaterial that can be engineered to encapsulate, stabilize, and organize guest molecules, nanoparticles, and biological moieties.. In this study, for the first time, the combined interactions of DNA strands with porous protein crystals are quantitatively measured by highresolution atomic force microscopy (AFM) and chemical force microscopy.. The surface structure of protein crystals with unusually large pores was observed in liquid via high-resolution AFM.



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    Image Search Results


    Fig. 1. AlkB homolog 5 (ALKBH5) overexpression inhibited prostate cancer (PCa) cell viability and proliferation. (A) Expression of ALKBH5 in PCa cells (LNCaP, DU145, PC3) or normal cells (RWPE-1) was detected using quantitative real-time polymerase chain

    Journal: Discovery Medicine

    Article Title: ALKBH5 Suppresses Autophagy in Prostate Cancer Cells via Inhibiting m6A-Modification of TSPAN1 mRNA

    doi: 10.24976/discov.med.202436180.5

    Figure Lengend Snippet: Fig. 1. AlkB homolog 5 (ALKBH5) overexpression inhibited prostate cancer (PCa) cell viability and proliferation. (A) Expression of ALKBH5 in PCa cells (LNCaP, DU145, PC3) or normal cells (RWPE-1) was detected using quantitative real-time polymerase chain

    Article Snippet: In addition, cells in the ALKBH5 + Rapamycin group were transfected with ALKBH5 and then treated with rapamycin (250 nM, dissolved in DMSO; T1537, TargetMol, Shanghai, China) for 1 h [24], and cells in the ALKBH5 + TSPAN1 group were transfected with ALKBH5 and TSPAN1 overexpression plasmid. qRT-PCR The qRT-PCR assays were conducted for determination of TSPAN1 and ALKBH5 expression, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal reference.

    Techniques: Over Expression, Expressing

    Fig. 2. ALKBH5 overexpression suppressed autophagy-associated proteins in PCa cells. (A–E) Autophagy-associated proteins

    Journal: Discovery Medicine

    Article Title: ALKBH5 Suppresses Autophagy in Prostate Cancer Cells via Inhibiting m6A-Modification of TSPAN1 mRNA

    doi: 10.24976/discov.med.202436180.5

    Figure Lengend Snippet: Fig. 2. ALKBH5 overexpression suppressed autophagy-associated proteins in PCa cells. (A–E) Autophagy-associated proteins

    Article Snippet: In addition, cells in the ALKBH5 + Rapamycin group were transfected with ALKBH5 and then treated with rapamycin (250 nM, dissolved in DMSO; T1537, TargetMol, Shanghai, China) for 1 h [24], and cells in the ALKBH5 + TSPAN1 group were transfected with ALKBH5 and TSPAN1 overexpression plasmid. qRT-PCR The qRT-PCR assays were conducted for determination of TSPAN1 and ALKBH5 expression, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal reference.

    Techniques: Over Expression

    Fig. 3. ALKBH5 reduced the N6-methyladenosine (m6A) level of Tetraspanin 1 (TSPAN1) to affect PCa cell viability. (A) Methy-

    Journal: Discovery Medicine

    Article Title: ALKBH5 Suppresses Autophagy in Prostate Cancer Cells via Inhibiting m6A-Modification of TSPAN1 mRNA

    doi: 10.24976/discov.med.202436180.5

    Figure Lengend Snippet: Fig. 3. ALKBH5 reduced the N6-methyladenosine (m6A) level of Tetraspanin 1 (TSPAN1) to affect PCa cell viability. (A) Methy-

    Article Snippet: In addition, cells in the ALKBH5 + Rapamycin group were transfected with ALKBH5 and then treated with rapamycin (250 nM, dissolved in DMSO; T1537, TargetMol, Shanghai, China) for 1 h [24], and cells in the ALKBH5 + TSPAN1 group were transfected with ALKBH5 and TSPAN1 overexpression plasmid. qRT-PCR The qRT-PCR assays were conducted for determination of TSPAN1 and ALKBH5 expression, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal reference.

    Techniques:

    Fig. 4. The effect of ALKBH5 and/or TSPAN1 overexpression on the proliferation of LNCaP and DU145 cells was assessed using EdU staining (EdU: red; Hoechst 33342: blue). (A–C) TSPAN1 overexpression promoted the number of EdU-positive cells, while

    Journal: Discovery Medicine

    Article Title: ALKBH5 Suppresses Autophagy in Prostate Cancer Cells via Inhibiting m6A-Modification of TSPAN1 mRNA

    doi: 10.24976/discov.med.202436180.5

    Figure Lengend Snippet: Fig. 4. The effect of ALKBH5 and/or TSPAN1 overexpression on the proliferation of LNCaP and DU145 cells was assessed using EdU staining (EdU: red; Hoechst 33342: blue). (A–C) TSPAN1 overexpression promoted the number of EdU-positive cells, while

    Article Snippet: In addition, cells in the ALKBH5 + Rapamycin group were transfected with ALKBH5 and then treated with rapamycin (250 nM, dissolved in DMSO; T1537, TargetMol, Shanghai, China) for 1 h [24], and cells in the ALKBH5 + TSPAN1 group were transfected with ALKBH5 and TSPAN1 overexpression plasmid. qRT-PCR The qRT-PCR assays were conducted for determination of TSPAN1 and ALKBH5 expression, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal reference.

    Techniques: Over Expression, Staining

    Fig. 5. Overexpression of ALKBH5 and/or TSPAN1 influenced autophagy-related protein expression in PCa cells. (A–E) LC3B-I,

    Journal: Discovery Medicine

    Article Title: ALKBH5 Suppresses Autophagy in Prostate Cancer Cells via Inhibiting m6A-Modification of TSPAN1 mRNA

    doi: 10.24976/discov.med.202436180.5

    Figure Lengend Snippet: Fig. 5. Overexpression of ALKBH5 and/or TSPAN1 influenced autophagy-related protein expression in PCa cells. (A–E) LC3B-I,

    Article Snippet: In addition, cells in the ALKBH5 + Rapamycin group were transfected with ALKBH5 and then treated with rapamycin (250 nM, dissolved in DMSO; T1537, TargetMol, Shanghai, China) for 1 h [24], and cells in the ALKBH5 + TSPAN1 group were transfected with ALKBH5 and TSPAN1 overexpression plasmid. qRT-PCR The qRT-PCR assays were conducted for determination of TSPAN1 and ALKBH5 expression, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal reference.

    Techniques: Over Expression, Expressing