Single-particle Tracking:Article Title: Escherichia coli Free Radical-Based Killing Mechanism Driven by a Unique Combination of Iron Restriction and Certain Antibiotics
Article Snippet: All experiments were performed in brain-heart infusion (BHI) medium, 97 or Cation-Adjusted Mueller-Hinton Broth (CAMHB, both from Teknova). .. The following 98 antibiotics and reagents were used at concentrations indicated in the figure legends: ampicillin 99 (AMP, from Amresco), cefotaxime (CTX, from Chem-Impex), chloramphenicol (CHL, from 100 EMD), ciprofloxacin (CIP, from Cellgro), daptomycin (DAP, from SellechChem), gentamicin 101 (GEN, from Chem-Impex), levofloxacin (LVX, from TCI), meropenem (MEM, from TCI) 102 methicillin (MET, from USP), spectinomycin (SPT, from MP), trimethoprim (TMP, from Chem-103 Impex), vancomycin (VAN, from RPI), deferiprone (DFP) and deferoxamine (DFX, both from 104 Sigma-Aldrich), 2 2' dipyridyl (DIP, from Alfa Aesar), ortho-Nitrophenyl-β-galactoside (ONPG 105 from Thermo Fisher Scientific), 2,7-Dichlorodihydrofluorescein diacetate (DCFH-DA from 106 Calbiochem), Glutathione (from Calbiochem), L-Ascorbic acid (from G-Biosciences), propidium 107 iodide (PI, from Fisher Scientific), Tween20, and TritonX100 (both from Sigma-Aldrich). ..
Article Title: Escherichia coli Free Radical-Based Killing Mechanism Driven by a Unique Combination of Iron Restriction and Certain Antibiotics
Article Snippet: All experiments were performed with brain heart infusion (BHI) medium or cation-adjusted Mueller-Hinton broth (CAMHB; both from Teknova). .. The following antibiotics and reagents were used at the concentrations indicated in the figure legends: ampicillin (AMP; from Amresco), cefotaxime (CTX; from Chem-Impex), CHL (from EMD), ciprofloxacin (CIP; from Cellgro), daptomycin (from SellechChem), gentamicin (GEN; from Chem-Impex), levofloxacin (LVX; from TCI), meropenem (MEM; from TCI) MET (from USP), spectinomycin (SPT; from MP), trimethoprim (TMP; from Chem-Impex), vancomycin (VAN; from RPI), deferiprone (from Sigma-Aldrich), deferoxamine (from Sigma-Aldrich), 2,2′-dipyridyl (from Alfa Aesar), ortho -nitrophenyl-β- d -galactopyranoside (ONPG; from Thermo Fisher Scientific), 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA; from Calbiochem), glutathione (from Calbiochem), l -ascorbic acid (from G-Biosciences), propidium iodide (PI; from Fisher Scientific), Tween 20 (from Sigma-Aldrich), and Triton X-100 (from Sigma-Aldrich). ..
other:Article Title: Repurposing Thiram and Disulfiram as Antibacterial Agents for Multidrug-Resistant Staphylococcus aureus Infections
Article Snippet: Vancomycin hydrochloride (VAN), oxacillin sodium (OXA), and cefotaxime sodium were acquired from Chem-Impex International (Wood Dale, IL).
Article Title: Repurposing Thiram and Disulfiram as Antibacterial Agents for Multidrug-Resistant Staphylococcus aureus Infections
Article Snippet: Vancomycin hydrochloride 246 (VAN), oxacillin sodium (OXA), and cefotaxime sodium (CTX) were acquired from Chem-Impex 247 International (Wood Dale, IL, USA).
Virus:Article Title: A fungal transcription factor converts a beneficial root endophyte into an anthracnose leaf pathogen.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Escherichia coli Stellar Competent Cells TaKaRa Cat#636763 Agrobacterium tumefaciens C58C1 Tsuji et al.62 N/A Agrobacterium tumefaciens GV3101 Stored in the lab N/A Chemicals, peptides, and recombinant proteins Acetosyringone Fuji Film Cat#BD12063 Hygromycin B Fuji Film Cat085-06153 Cefotaxime CHEM-IMPEX Cat#01539 Spectinomycin Fuji Film Cat#195-11531 Nourseothricin Jena Bioscience Cat#AB-102XL KOD One PCR Master Mix TOYOBO Cat#KMM-201 PrimeSTAR GXL DNA Polymerase TaKaRa Cat#R050A In-Fusion HD Cloning Kit TaKaRa Cat#639650 pENTR Directional TOPO Cloning Kits Invitrogen Cat#45-0218 NEB Quick ligation kit NEB Cat#M2200S Calcofluor White Stain Sigma Cat#18909-100ML-F SCRI Renaissance 2200 Stain Renaissance Chemicals N/A Driselase Sigma Cat#D9515 Yatalase-Plus TaKaRa Cat#T030 SalI NEB Cat#R3138S KpnI NEB Cat#R3142S HindIII NEB Cat#R3104S XhoI NEB Cat#R0146S BsaI NEB Cat#R3733S WGA-FITC conjugate Sigma Cat#L4895 Murashige and Skoog salts FUJIFILM Wako Pure Chemicals Cat#392-00591 Mycological peptone OXOID Cat#LP0040B INA Agar INA food company Cat#BA-10 Difco agar Difco Cat#214010 Difco Potato Dextrose Agar Difco Cat#213400 Critical commercial assays ATAC-Seq Kit Active Motif Cat#53150 Dual-Luciferase Reporter Assay System Promega Cat#E1910 Beta DEX 120 fused silica capillary column Supelco Cat#24304 ACQUITY UPLC BEH C18 (2.1 x 50 mm) Waters Cat#186002350 Nucleospin RNA Plant Macherey-Nagel Cat#740949.250 NucleoBond Xtra Midi Macherey-Nagel Cat#740410.10 PrimeScript RT Master Mix TaKaRa Cat#RR037A THUNDERBIRD Next SYBR qPCR Mix TOYOBO Cat#QPX-201 Deposited data in vitro fungal RNA-seq raw sequencing data DDBJ DDBJ: PRJDB17668 in planta RNA-seq raw sequencing data DDBJ DDBJ; PRJDB18366 ATAC-seq raw sequencing data DDBJ DDBJ: PRJDB18383 (Continued on next page) e1 Current Biology 35, 1–17.e1–e6, May 5, 2025 ..
Recombinant:Article Title: A fungal transcription factor converts a beneficial root endophyte into an anthracnose leaf pathogen.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Escherichia coli Stellar Competent Cells TaKaRa Cat#636763 Agrobacterium tumefaciens C58C1 Tsuji et al.62 N/A Agrobacterium tumefaciens GV3101 Stored in the lab N/A Chemicals, peptides, and recombinant proteins Acetosyringone Fuji Film Cat#BD12063 Hygromycin B Fuji Film Cat085-06153 Cefotaxime CHEM-IMPEX Cat#01539 Spectinomycin Fuji Film Cat#195-11531 Nourseothricin Jena Bioscience Cat#AB-102XL KOD One PCR Master Mix TOYOBO Cat#KMM-201 PrimeSTAR GXL DNA Polymerase TaKaRa Cat#R050A In-Fusion HD Cloning Kit TaKaRa Cat#639650 pENTR Directional TOPO Cloning Kits Invitrogen Cat#45-0218 NEB Quick ligation kit NEB Cat#M2200S Calcofluor White Stain Sigma Cat#18909-100ML-F SCRI Renaissance 2200 Stain Renaissance Chemicals N/A Driselase Sigma Cat#D9515 Yatalase-Plus TaKaRa Cat#T030 SalI NEB Cat#R3138S KpnI NEB Cat#R3142S HindIII NEB Cat#R3104S XhoI NEB Cat#R0146S BsaI NEB Cat#R3733S WGA-FITC conjugate Sigma Cat#L4895 Murashige and Skoog salts FUJIFILM Wako Pure Chemicals Cat#392-00591 Mycological peptone OXOID Cat#LP0040B INA Agar INA food company Cat#BA-10 Difco agar Difco Cat#214010 Difco Potato Dextrose Agar Difco Cat#213400 Critical commercial assays ATAC-Seq Kit Active Motif Cat#53150 Dual-Luciferase Reporter Assay System Promega Cat#E1910 Beta DEX 120 fused silica capillary column Supelco Cat#24304 ACQUITY UPLC BEH C18 (2.1 x 50 mm) Waters Cat#186002350 Nucleospin RNA Plant Macherey-Nagel Cat#740949.250 NucleoBond Xtra Midi Macherey-Nagel Cat#740410.10 PrimeScript RT Master Mix TaKaRa Cat#RR037A THUNDERBIRD Next SYBR qPCR Mix TOYOBO Cat#QPX-201 Deposited data in vitro fungal RNA-seq raw sequencing data DDBJ DDBJ: PRJDB17668 in planta RNA-seq raw sequencing data DDBJ DDBJ; PRJDB18366 ATAC-seq raw sequencing data DDBJ DDBJ: PRJDB18383 (Continued on next page) e1 Current Biology 35, 1–17.e1–e6, May 5, 2025 ..
Polymerase Chain Reaction:Article Title: A fungal transcription factor converts a beneficial root endophyte into an anthracnose leaf pathogen.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Escherichia coli Stellar Competent Cells TaKaRa Cat#636763 Agrobacterium tumefaciens C58C1 Tsuji et al.62 N/A Agrobacterium tumefaciens GV3101 Stored in the lab N/A Chemicals, peptides, and recombinant proteins Acetosyringone Fuji Film Cat#BD12063 Hygromycin B Fuji Film Cat085-06153 Cefotaxime CHEM-IMPEX Cat#01539 Spectinomycin Fuji Film Cat#195-11531 Nourseothricin Jena Bioscience Cat#AB-102XL KOD One PCR Master Mix TOYOBO Cat#KMM-201 PrimeSTAR GXL DNA Polymerase TaKaRa Cat#R050A In-Fusion HD Cloning Kit TaKaRa Cat#639650 pENTR Directional TOPO Cloning Kits Invitrogen Cat#45-0218 NEB Quick ligation kit NEB Cat#M2200S Calcofluor White Stain Sigma Cat#18909-100ML-F SCRI Renaissance 2200 Stain Renaissance Chemicals N/A Driselase Sigma Cat#D9515 Yatalase-Plus TaKaRa Cat#T030 SalI NEB Cat#R3138S KpnI NEB Cat#R3142S HindIII NEB Cat#R3104S XhoI NEB Cat#R0146S BsaI NEB Cat#R3733S WGA-FITC conjugate Sigma Cat#L4895 Murashige and Skoog salts FUJIFILM Wako Pure Chemicals Cat#392-00591 Mycological peptone OXOID Cat#LP0040B INA Agar INA food company Cat#BA-10 Difco agar Difco Cat#214010 Difco Potato Dextrose Agar Difco Cat#213400 Critical commercial assays ATAC-Seq Kit Active Motif Cat#53150 Dual-Luciferase Reporter Assay System Promega Cat#E1910 Beta DEX 120 fused silica capillary column Supelco Cat#24304 ACQUITY UPLC BEH C18 (2.1 x 50 mm) Waters Cat#186002350 Nucleospin RNA Plant Macherey-Nagel Cat#740949.250 NucleoBond Xtra Midi Macherey-Nagel Cat#740410.10 PrimeScript RT Master Mix TaKaRa Cat#RR037A THUNDERBIRD Next SYBR qPCR Mix TOYOBO Cat#QPX-201 Deposited data in vitro fungal RNA-seq raw sequencing data DDBJ DDBJ: PRJDB17668 in planta RNA-seq raw sequencing data DDBJ DDBJ; PRJDB18366 ATAC-seq raw sequencing data DDBJ DDBJ: PRJDB18383 (Continued on next page) e1 Current Biology 35, 1–17.e1–e6, May 5, 2025 ..
Cloning:Article Title: A fungal transcription factor converts a beneficial root endophyte into an anthracnose leaf pathogen.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Escherichia coli Stellar Competent Cells TaKaRa Cat#636763 Agrobacterium tumefaciens C58C1 Tsuji et al.62 N/A Agrobacterium tumefaciens GV3101 Stored in the lab N/A Chemicals, peptides, and recombinant proteins Acetosyringone Fuji Film Cat#BD12063 Hygromycin B Fuji Film Cat085-06153 Cefotaxime CHEM-IMPEX Cat#01539 Spectinomycin Fuji Film Cat#195-11531 Nourseothricin Jena Bioscience Cat#AB-102XL KOD One PCR Master Mix TOYOBO Cat#KMM-201 PrimeSTAR GXL DNA Polymerase TaKaRa Cat#R050A In-Fusion HD Cloning Kit TaKaRa Cat#639650 pENTR Directional TOPO Cloning Kits Invitrogen Cat#45-0218 NEB Quick ligation kit NEB Cat#M2200S Calcofluor White Stain Sigma Cat#18909-100ML-F SCRI Renaissance 2200 Stain Renaissance Chemicals N/A Driselase Sigma Cat#D9515 Yatalase-Plus TaKaRa Cat#T030 SalI NEB Cat#R3138S KpnI NEB Cat#R3142S HindIII NEB Cat#R3104S XhoI NEB Cat#R0146S BsaI NEB Cat#R3733S WGA-FITC conjugate Sigma Cat#L4895 Murashige and Skoog salts FUJIFILM Wako Pure Chemicals Cat#392-00591 Mycological peptone OXOID Cat#LP0040B INA Agar INA food company Cat#BA-10 Difco agar Difco Cat#214010 Difco Potato Dextrose Agar Difco Cat#213400 Critical commercial assays ATAC-Seq Kit Active Motif Cat#53150 Dual-Luciferase Reporter Assay System Promega Cat#E1910 Beta DEX 120 fused silica capillary column Supelco Cat#24304 ACQUITY UPLC BEH C18 (2.1 x 50 mm) Waters Cat#186002350 Nucleospin RNA Plant Macherey-Nagel Cat#740949.250 NucleoBond Xtra Midi Macherey-Nagel Cat#740410.10 PrimeScript RT Master Mix TaKaRa Cat#RR037A THUNDERBIRD Next SYBR qPCR Mix TOYOBO Cat#QPX-201 Deposited data in vitro fungal RNA-seq raw sequencing data DDBJ DDBJ: PRJDB17668 in planta RNA-seq raw sequencing data DDBJ DDBJ; PRJDB18366 ATAC-seq raw sequencing data DDBJ DDBJ: PRJDB18383 (Continued on next page) e1 Current Biology 35, 1–17.e1–e6, May 5, 2025 ..
Ligation:Article Title: A fungal transcription factor converts a beneficial root endophyte into an anthracnose leaf pathogen.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Escherichia coli Stellar Competent Cells TaKaRa Cat#636763 Agrobacterium tumefaciens C58C1 Tsuji et al.62 N/A Agrobacterium tumefaciens GV3101 Stored in the lab N/A Chemicals, peptides, and recombinant proteins Acetosyringone Fuji Film Cat#BD12063 Hygromycin B Fuji Film Cat085-06153 Cefotaxime CHEM-IMPEX Cat#01539 Spectinomycin Fuji Film Cat#195-11531 Nourseothricin Jena Bioscience Cat#AB-102XL KOD One PCR Master Mix TOYOBO Cat#KMM-201 PrimeSTAR GXL DNA Polymerase TaKaRa Cat#R050A In-Fusion HD Cloning Kit TaKaRa Cat#639650 pENTR Directional TOPO Cloning Kits Invitrogen Cat#45-0218 NEB Quick ligation kit NEB Cat#M2200S Calcofluor White Stain Sigma Cat#18909-100ML-F SCRI Renaissance 2200 Stain Renaissance Chemicals N/A Driselase Sigma Cat#D9515 Yatalase-Plus TaKaRa Cat#T030 SalI NEB Cat#R3138S KpnI NEB Cat#R3142S HindIII NEB Cat#R3104S XhoI NEB Cat#R0146S BsaI NEB Cat#R3733S WGA-FITC conjugate Sigma Cat#L4895 Murashige and Skoog salts FUJIFILM Wako Pure Chemicals Cat#392-00591 Mycological peptone OXOID Cat#LP0040B INA Agar INA food company Cat#BA-10 Difco agar Difco Cat#214010 Difco Potato Dextrose Agar Difco Cat#213400 Critical commercial assays ATAC-Seq Kit Active Motif Cat#53150 Dual-Luciferase Reporter Assay System Promega Cat#E1910 Beta DEX 120 fused silica capillary column Supelco Cat#24304 ACQUITY UPLC BEH C18 (2.1 x 50 mm) Waters Cat#186002350 Nucleospin RNA Plant Macherey-Nagel Cat#740949.250 NucleoBond Xtra Midi Macherey-Nagel Cat#740410.10 PrimeScript RT Master Mix TaKaRa Cat#RR037A THUNDERBIRD Next SYBR qPCR Mix TOYOBO Cat#QPX-201 Deposited data in vitro fungal RNA-seq raw sequencing data DDBJ DDBJ: PRJDB17668 in planta RNA-seq raw sequencing data DDBJ DDBJ; PRJDB18366 ATAC-seq raw sequencing data DDBJ DDBJ: PRJDB18383 (Continued on next page) e1 Current Biology 35, 1–17.e1–e6, May 5, 2025 ..
Staining:Article Title: A fungal transcription factor converts a beneficial root endophyte into an anthracnose leaf pathogen.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Escherichia coli Stellar Competent Cells TaKaRa Cat#636763 Agrobacterium tumefaciens C58C1 Tsuji et al.62 N/A Agrobacterium tumefaciens GV3101 Stored in the lab N/A Chemicals, peptides, and recombinant proteins Acetosyringone Fuji Film Cat#BD12063 Hygromycin B Fuji Film Cat085-06153 Cefotaxime CHEM-IMPEX Cat#01539 Spectinomycin Fuji Film Cat#195-11531 Nourseothricin Jena Bioscience Cat#AB-102XL KOD One PCR Master Mix TOYOBO Cat#KMM-201 PrimeSTAR GXL DNA Polymerase TaKaRa Cat#R050A In-Fusion HD Cloning Kit TaKaRa Cat#639650 pENTR Directional TOPO Cloning Kits Invitrogen Cat#45-0218 NEB Quick ligation kit NEB Cat#M2200S Calcofluor White Stain Sigma Cat#18909-100ML-F SCRI Renaissance 2200 Stain Renaissance Chemicals N/A Driselase Sigma Cat#D9515 Yatalase-Plus TaKaRa Cat#T030 SalI NEB Cat#R3138S KpnI NEB Cat#R3142S HindIII NEB Cat#R3104S XhoI NEB Cat#R0146S BsaI NEB Cat#R3733S WGA-FITC conjugate Sigma Cat#L4895 Murashige and Skoog salts FUJIFILM Wako Pure Chemicals Cat#392-00591 Mycological peptone OXOID Cat#LP0040B INA Agar INA food company Cat#BA-10 Difco agar Difco Cat#214010 Difco Potato Dextrose Agar Difco Cat#213400 Critical commercial assays ATAC-Seq Kit Active Motif Cat#53150 Dual-Luciferase Reporter Assay System Promega Cat#E1910 Beta DEX 120 fused silica capillary column Supelco Cat#24304 ACQUITY UPLC BEH C18 (2.1 x 50 mm) Waters Cat#186002350 Nucleospin RNA Plant Macherey-Nagel Cat#740949.250 NucleoBond Xtra Midi Macherey-Nagel Cat#740410.10 PrimeScript RT Master Mix TaKaRa Cat#RR037A THUNDERBIRD Next SYBR qPCR Mix TOYOBO Cat#QPX-201 Deposited data in vitro fungal RNA-seq raw sequencing data DDBJ DDBJ: PRJDB17668 in planta RNA-seq raw sequencing data DDBJ DDBJ; PRJDB18366 ATAC-seq raw sequencing data DDBJ DDBJ: PRJDB18383 (Continued on next page) e1 Current Biology 35, 1–17.e1–e6, May 5, 2025 ..
Whole Genome Amplification:Article Title: A fungal transcription factor converts a beneficial root endophyte into an anthracnose leaf pathogen.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Escherichia coli Stellar Competent Cells TaKaRa Cat#636763 Agrobacterium tumefaciens C58C1 Tsuji et al.62 N/A Agrobacterium tumefaciens GV3101 Stored in the lab N/A Chemicals, peptides, and recombinant proteins Acetosyringone Fuji Film Cat#BD12063 Hygromycin B Fuji Film Cat085-06153 Cefotaxime CHEM-IMPEX Cat#01539 Spectinomycin Fuji Film Cat#195-11531 Nourseothricin Jena Bioscience Cat#AB-102XL KOD One PCR Master Mix TOYOBO Cat#KMM-201 PrimeSTAR GXL DNA Polymerase TaKaRa Cat#R050A In-Fusion HD Cloning Kit TaKaRa Cat#639650 pENTR Directional TOPO Cloning Kits Invitrogen Cat#45-0218 NEB Quick ligation kit NEB Cat#M2200S Calcofluor White Stain Sigma Cat#18909-100ML-F SCRI Renaissance 2200 Stain Renaissance Chemicals N/A Driselase Sigma Cat#D9515 Yatalase-Plus TaKaRa Cat#T030 SalI NEB Cat#R3138S KpnI NEB Cat#R3142S HindIII NEB Cat#R3104S XhoI NEB Cat#R0146S BsaI NEB Cat#R3733S WGA-FITC conjugate Sigma Cat#L4895 Murashige and Skoog salts FUJIFILM Wako Pure Chemicals Cat#392-00591 Mycological peptone OXOID Cat#LP0040B INA Agar INA food company Cat#BA-10 Difco agar Difco Cat#214010 Difco Potato Dextrose Agar Difco Cat#213400 Critical commercial assays ATAC-Seq Kit Active Motif Cat#53150 Dual-Luciferase Reporter Assay System Promega Cat#E1910 Beta DEX 120 fused silica capillary column Supelco Cat#24304 ACQUITY UPLC BEH C18 (2.1 x 50 mm) Waters Cat#186002350 Nucleospin RNA Plant Macherey-Nagel Cat#740949.250 NucleoBond Xtra Midi Macherey-Nagel Cat#740410.10 PrimeScript RT Master Mix TaKaRa Cat#RR037A THUNDERBIRD Next SYBR qPCR Mix TOYOBO Cat#QPX-201 Deposited data in vitro fungal RNA-seq raw sequencing data DDBJ DDBJ: PRJDB17668 in planta RNA-seq raw sequencing data DDBJ DDBJ; PRJDB18366 ATAC-seq raw sequencing data DDBJ DDBJ: PRJDB18383 (Continued on next page) e1 Current Biology 35, 1–17.e1–e6, May 5, 2025 ..
Reporter Assay:Article Title: A fungal transcription factor converts a beneficial root endophyte into an anthracnose leaf pathogen.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Escherichia coli Stellar Competent Cells TaKaRa Cat#636763 Agrobacterium tumefaciens C58C1 Tsuji et al.62 N/A Agrobacterium tumefaciens GV3101 Stored in the lab N/A Chemicals, peptides, and recombinant proteins Acetosyringone Fuji Film Cat#BD12063 Hygromycin B Fuji Film Cat085-06153 Cefotaxime CHEM-IMPEX Cat#01539 Spectinomycin Fuji Film Cat#195-11531 Nourseothricin Jena Bioscience Cat#AB-102XL KOD One PCR Master Mix TOYOBO Cat#KMM-201 PrimeSTAR GXL DNA Polymerase TaKaRa Cat#R050A In-Fusion HD Cloning Kit TaKaRa Cat#639650 pENTR Directional TOPO Cloning Kits Invitrogen Cat#45-0218 NEB Quick ligation kit NEB Cat#M2200S Calcofluor White Stain Sigma Cat#18909-100ML-F SCRI Renaissance 2200 Stain Renaissance Chemicals N/A Driselase Sigma Cat#D9515 Yatalase-Plus TaKaRa Cat#T030 SalI NEB Cat#R3138S KpnI NEB Cat#R3142S HindIII NEB Cat#R3104S XhoI NEB Cat#R0146S BsaI NEB Cat#R3733S WGA-FITC conjugate Sigma Cat#L4895 Murashige and Skoog salts FUJIFILM Wako Pure Chemicals Cat#392-00591 Mycological peptone OXOID Cat#LP0040B INA Agar INA food company Cat#BA-10 Difco agar Difco Cat#214010 Difco Potato Dextrose Agar Difco Cat#213400 Critical commercial assays ATAC-Seq Kit Active Motif Cat#53150 Dual-Luciferase Reporter Assay System Promega Cat#E1910 Beta DEX 120 fused silica capillary column Supelco Cat#24304 ACQUITY UPLC BEH C18 (2.1 x 50 mm) Waters Cat#186002350 Nucleospin RNA Plant Macherey-Nagel Cat#740949.250 NucleoBond Xtra Midi Macherey-Nagel Cat#740410.10 PrimeScript RT Master Mix TaKaRa Cat#RR037A THUNDERBIRD Next SYBR qPCR Mix TOYOBO Cat#QPX-201 Deposited data in vitro fungal RNA-seq raw sequencing data DDBJ DDBJ: PRJDB17668 in planta RNA-seq raw sequencing data DDBJ DDBJ; PRJDB18366 ATAC-seq raw sequencing data DDBJ DDBJ: PRJDB18383 (Continued on next page) e1 Current Biology 35, 1–17.e1–e6, May 5, 2025 ..
Real-time Polymerase Chain Reaction:Article Title: A fungal transcription factor converts a beneficial root endophyte into an anthracnose leaf pathogen.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Escherichia coli Stellar Competent Cells TaKaRa Cat#636763 Agrobacterium tumefaciens C58C1 Tsuji et al.62 N/A Agrobacterium tumefaciens GV3101 Stored in the lab N/A Chemicals, peptides, and recombinant proteins Acetosyringone Fuji Film Cat#BD12063 Hygromycin B Fuji Film Cat085-06153 Cefotaxime CHEM-IMPEX Cat#01539 Spectinomycin Fuji Film Cat#195-11531 Nourseothricin Jena Bioscience Cat#AB-102XL KOD One PCR Master Mix TOYOBO Cat#KMM-201 PrimeSTAR GXL DNA Polymerase TaKaRa Cat#R050A In-Fusion HD Cloning Kit TaKaRa Cat#639650 pENTR Directional TOPO Cloning Kits Invitrogen Cat#45-0218 NEB Quick ligation kit NEB Cat#M2200S Calcofluor White Stain Sigma Cat#18909-100ML-F SCRI Renaissance 2200 Stain Renaissance Chemicals N/A Driselase Sigma Cat#D9515 Yatalase-Plus TaKaRa Cat#T030 SalI NEB Cat#R3138S KpnI NEB Cat#R3142S HindIII NEB Cat#R3104S XhoI NEB Cat#R0146S BsaI NEB Cat#R3733S WGA-FITC conjugate Sigma Cat#L4895 Murashige and Skoog salts FUJIFILM Wako Pure Chemicals Cat#392-00591 Mycological peptone OXOID Cat#LP0040B INA Agar INA food company Cat#BA-10 Difco agar Difco Cat#214010 Difco Potato Dextrose Agar Difco Cat#213400 Critical commercial assays ATAC-Seq Kit Active Motif Cat#53150 Dual-Luciferase Reporter Assay System Promega Cat#E1910 Beta DEX 120 fused silica capillary column Supelco Cat#24304 ACQUITY UPLC BEH C18 (2.1 x 50 mm) Waters Cat#186002350 Nucleospin RNA Plant Macherey-Nagel Cat#740949.250 NucleoBond Xtra Midi Macherey-Nagel Cat#740410.10 PrimeScript RT Master Mix TaKaRa Cat#RR037A THUNDERBIRD Next SYBR qPCR Mix TOYOBO Cat#QPX-201 Deposited data in vitro fungal RNA-seq raw sequencing data DDBJ DDBJ: PRJDB17668 in planta RNA-seq raw sequencing data DDBJ DDBJ; PRJDB18366 ATAC-seq raw sequencing data DDBJ DDBJ: PRJDB18383 (Continued on next page) e1 Current Biology 35, 1–17.e1–e6, May 5, 2025 ..
In Vitro:Article Title: A fungal transcription factor converts a beneficial root endophyte into an anthracnose leaf pathogen.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Escherichia coli Stellar Competent Cells TaKaRa Cat#636763 Agrobacterium tumefaciens C58C1 Tsuji et al.62 N/A Agrobacterium tumefaciens GV3101 Stored in the lab N/A Chemicals, peptides, and recombinant proteins Acetosyringone Fuji Film Cat#BD12063 Hygromycin B Fuji Film Cat085-06153 Cefotaxime CHEM-IMPEX Cat#01539 Spectinomycin Fuji Film Cat#195-11531 Nourseothricin Jena Bioscience Cat#AB-102XL KOD One PCR Master Mix TOYOBO Cat#KMM-201 PrimeSTAR GXL DNA Polymerase TaKaRa Cat#R050A In-Fusion HD Cloning Kit TaKaRa Cat#639650 pENTR Directional TOPO Cloning Kits Invitrogen Cat#45-0218 NEB Quick ligation kit NEB Cat#M2200S Calcofluor White Stain Sigma Cat#18909-100ML-F SCRI Renaissance 2200 Stain Renaissance Chemicals N/A Driselase Sigma Cat#D9515 Yatalase-Plus TaKaRa Cat#T030 SalI NEB Cat#R3138S KpnI NEB Cat#R3142S HindIII NEB Cat#R3104S XhoI NEB Cat#R0146S BsaI NEB Cat#R3733S WGA-FITC conjugate Sigma Cat#L4895 Murashige and Skoog salts FUJIFILM Wako Pure Chemicals Cat#392-00591 Mycological peptone OXOID Cat#LP0040B INA Agar INA food company Cat#BA-10 Difco agar Difco Cat#214010 Difco Potato Dextrose Agar Difco Cat#213400 Critical commercial assays ATAC-Seq Kit Active Motif Cat#53150 Dual-Luciferase Reporter Assay System Promega Cat#E1910 Beta DEX 120 fused silica capillary column Supelco Cat#24304 ACQUITY UPLC BEH C18 (2.1 x 50 mm) Waters Cat#186002350 Nucleospin RNA Plant Macherey-Nagel Cat#740949.250 NucleoBond Xtra Midi Macherey-Nagel Cat#740410.10 PrimeScript RT Master Mix TaKaRa Cat#RR037A THUNDERBIRD Next SYBR qPCR Mix TOYOBO Cat#QPX-201 Deposited data in vitro fungal RNA-seq raw sequencing data DDBJ DDBJ: PRJDB17668 in planta RNA-seq raw sequencing data DDBJ DDBJ; PRJDB18366 ATAC-seq raw sequencing data DDBJ DDBJ: PRJDB18383 (Continued on next page) e1 Current Biology 35, 1–17.e1–e6, May 5, 2025 ..
RNA Sequencing:Article Title: A fungal transcription factor converts a beneficial root endophyte into an anthracnose leaf pathogen.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Escherichia coli Stellar Competent Cells TaKaRa Cat#636763 Agrobacterium tumefaciens C58C1 Tsuji et al.62 N/A Agrobacterium tumefaciens GV3101 Stored in the lab N/A Chemicals, peptides, and recombinant proteins Acetosyringone Fuji Film Cat#BD12063 Hygromycin B Fuji Film Cat085-06153 Cefotaxime CHEM-IMPEX Cat#01539 Spectinomycin Fuji Film Cat#195-11531 Nourseothricin Jena Bioscience Cat#AB-102XL KOD One PCR Master Mix TOYOBO Cat#KMM-201 PrimeSTAR GXL DNA Polymerase TaKaRa Cat#R050A In-Fusion HD Cloning Kit TaKaRa Cat#639650 pENTR Directional TOPO Cloning Kits Invitrogen Cat#45-0218 NEB Quick ligation kit NEB Cat#M2200S Calcofluor White Stain Sigma Cat#18909-100ML-F SCRI Renaissance 2200 Stain Renaissance Chemicals N/A Driselase Sigma Cat#D9515 Yatalase-Plus TaKaRa Cat#T030 SalI NEB Cat#R3138S KpnI NEB Cat#R3142S HindIII NEB Cat#R3104S XhoI NEB Cat#R0146S BsaI NEB Cat#R3733S WGA-FITC conjugate Sigma Cat#L4895 Murashige and Skoog salts FUJIFILM Wako Pure Chemicals Cat#392-00591 Mycological peptone OXOID Cat#LP0040B INA Agar INA food company Cat#BA-10 Difco agar Difco Cat#214010 Difco Potato Dextrose Agar Difco Cat#213400 Critical commercial assays ATAC-Seq Kit Active Motif Cat#53150 Dual-Luciferase Reporter Assay System Promega Cat#E1910 Beta DEX 120 fused silica capillary column Supelco Cat#24304 ACQUITY UPLC BEH C18 (2.1 x 50 mm) Waters Cat#186002350 Nucleospin RNA Plant Macherey-Nagel Cat#740949.250 NucleoBond Xtra Midi Macherey-Nagel Cat#740410.10 PrimeScript RT Master Mix TaKaRa Cat#RR037A THUNDERBIRD Next SYBR qPCR Mix TOYOBO Cat#QPX-201 Deposited data in vitro fungal RNA-seq raw sequencing data DDBJ DDBJ: PRJDB17668 in planta RNA-seq raw sequencing data DDBJ DDBJ; PRJDB18366 ATAC-seq raw sequencing data DDBJ DDBJ: PRJDB18383 (Continued on next page) e1 Current Biology 35, 1–17.e1–e6, May 5, 2025 ..
Sequencing:Article Title: A fungal transcription factor converts a beneficial root endophyte into an anthracnose leaf pathogen.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Escherichia coli Stellar Competent Cells TaKaRa Cat#636763 Agrobacterium tumefaciens C58C1 Tsuji et al.62 N/A Agrobacterium tumefaciens GV3101 Stored in the lab N/A Chemicals, peptides, and recombinant proteins Acetosyringone Fuji Film Cat#BD12063 Hygromycin B Fuji Film Cat085-06153 Cefotaxime CHEM-IMPEX Cat#01539 Spectinomycin Fuji Film Cat#195-11531 Nourseothricin Jena Bioscience Cat#AB-102XL KOD One PCR Master Mix TOYOBO Cat#KMM-201 PrimeSTAR GXL DNA Polymerase TaKaRa Cat#R050A In-Fusion HD Cloning Kit TaKaRa Cat#639650 pENTR Directional TOPO Cloning Kits Invitrogen Cat#45-0218 NEB Quick ligation kit NEB Cat#M2200S Calcofluor White Stain Sigma Cat#18909-100ML-F SCRI Renaissance 2200 Stain Renaissance Chemicals N/A Driselase Sigma Cat#D9515 Yatalase-Plus TaKaRa Cat#T030 SalI NEB Cat#R3138S KpnI NEB Cat#R3142S HindIII NEB Cat#R3104S XhoI NEB Cat#R0146S BsaI NEB Cat#R3733S WGA-FITC conjugate Sigma Cat#L4895 Murashige and Skoog salts FUJIFILM Wako Pure Chemicals Cat#392-00591 Mycological peptone OXOID Cat#LP0040B INA Agar INA food company Cat#BA-10 Difco agar Difco Cat#214010 Difco Potato Dextrose Agar Difco Cat#213400 Critical commercial assays ATAC-Seq Kit Active Motif Cat#53150 Dual-Luciferase Reporter Assay System Promega Cat#E1910 Beta DEX 120 fused silica capillary column Supelco Cat#24304 ACQUITY UPLC BEH C18 (2.1 x 50 mm) Waters Cat#186002350 Nucleospin RNA Plant Macherey-Nagel Cat#740949.250 NucleoBond Xtra Midi Macherey-Nagel Cat#740410.10 PrimeScript RT Master Mix TaKaRa Cat#RR037A THUNDERBIRD Next SYBR qPCR Mix TOYOBO Cat#QPX-201 Deposited data in vitro fungal RNA-seq raw sequencing data DDBJ DDBJ: PRJDB17668 in planta RNA-seq raw sequencing data DDBJ DDBJ; PRJDB18366 ATAC-seq raw sequencing data DDBJ DDBJ: PRJDB18383 (Continued on next page) e1 Current Biology 35, 1–17.e1–e6, May 5, 2025 ..
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