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clotrimazole  (Chem Impex International)


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    Structured Review

    Chem Impex International clotrimazole
    Clotrimazole, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/01311/Clotrimazole/10__7554_slash_elife__92899-293-32-38
    Average 95 stars, based on 3 article reviews
    clotrimazole - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Evolution of haploid and diploid populations reveals common, strong, and variable pleiotropic effects in non-home environments
    Article Snippet: pre-culture was used to inoculate both replicate evolutions for each condition with ~5e7 cells (~400 µL). .. Conditions with 0.2 M NaCl (Fisher Scientific – BP358-212), 0.8 M NaCl, 21°C, 2% glycerol (Fisher – G33-4)+2% ethanol (Goldshield – 412804), fluconazole 13 µM (4 mg/L, Acros Organics #455480050), Clotrimazole 6 µM (or 2 mg/L, Chem-Impex International #01311) were performed with 48 hr transfers at Stanford. .. The YPD, SC, SC with 48 hr transfer, 37°C with 280 rpm shaking, pH buffered to 7.8, and pH adjusted to 3.8 wer

    Article Title: Evolution of haploid and diploid populations reveals common, strong, and variable pleiotropic effects in non-home environments
    Article Snippet: pre- culture was used to inoculate both replicate evolutions for each condition with ~5e7 cells (~400 µL). .. Conditions with 0.2 M NaCl (Fisher Scientific – BP358- 212), 0.8 M NaCl, 21°C, 2% glycerol (Fisher – G33- 4)+2% ethanol (Goldshield – 412804), fluconazole 13 µM (4 mg/L, Acros Organics #455480050), Clotrimazole 6 µM (or 2 mg/L, Chem- Impex International #01311) were performed with 48 hr transfers Chen, Johnson, Hérissant et al. eLife 2023;12:e92899. .. DOI: https://doi.org/10.7554/eLife.92899 15 of 35 at Stanford.

    Article Title: Far From Home: Evolution of haploid and diploid populations reveals common, strong, and variable pleiotropic effects in non-home environments
    Article Snippet: pre-culture was used to inoculate both replicate evolutions for each condition with ∼5e7 cells (∼400 µL). .. Conditions with 0.2M NaCl (Fisher Scientific - BP358-212), 0.8M NaCl, 21°C, 2% Glycerol (Fisher - G33-4) + 2 % Ethanol (Goldshield - 412804), Fluconazole 13 µM (4 mg/L, Acros Organics #455480050), Clotrimazole 6 µM (or 2 mg/L, Chem-Impex International #01311) were performed with 48 hour transfers at Stanford. .. The YPD, SC, SC with 48 hours transfer, 37°C with 280 rpm shaking, pH buffered to 7.8, and pH adjusted to 3.8

    Article Title: Antimicrobial compositions containing a synergistic combination of activated creatinine and an imidazole antifungal agent
    Article Snippet: Formulations of the invention were prepared containing clotrimazole (Chem-Impex International, Inc., Wood Dale, Ill.) and creatinine hydrochloride (Sigma-Aldrich Corporation, St. Louis, Mo.) in different molar ratios, in order to evaluate the potential effect of those ratios on antibacterial efficacy. .. T

    Isolation:

    Article Title: Evolution of haploid and diploid populations reveals common, strong, and variable pleiotropic effects in non-home environments
    Article Snippet: pre-culture was used to inoculate both replicate evolutions for each condition with ~5e7 cells (~400 µL). .. Conditions with 0.2 M NaCl (Fisher Scientific – BP358-212), 0.8 M NaCl, 21°C, 2% glycerol (Fisher – G33-4)+2% ethanol (Goldshield – 412804), fluconazole 13 µM (4 mg/L, Acros Organics #455480050), Clotrimazole 6 µM (or 2 mg/L, Chem-Impex International #01311) were performed with 48 hr transfers at Stanford. .. The YPD, SC, SC with 48 hr transfer, 37°C with 280 rpm shaking, pH buffered to 7.8, and pH adjusted to 3.8 wer

    Article Title: Evolution of haploid and diploid populations reveals common, strong, and variable pleiotropic effects in non-home environments
    Article Snippet: pre- culture was used to inoculate both replicate evolutions for each condition with ~5e7 cells (~400 µL). .. Conditions with 0.2 M NaCl (Fisher Scientific – BP358- 212), 0.8 M NaCl, 21°C, 2% glycerol (Fisher – G33- 4)+2% ethanol (Goldshield – 412804), fluconazole 13 µM (4 mg/L, Acros Organics #455480050), Clotrimazole 6 µM (or 2 mg/L, Chem- Impex International #01311) were performed with 48 hr transfers Chen, Johnson, Hérissant et al. eLife 2023;12:e92899. .. DOI: https://doi.org/10.7554/eLife.92899 15 of 35 at Stanford.

    Article Title: Far From Home: Evolution of haploid and diploid populations reveals common, strong, and variable pleiotropic effects in non-home environments
    Article Snippet: pre-culture was used to inoculate both replicate evolutions for each condition with ∼5e7 cells (∼400 µL). .. Conditions with 0.2M NaCl (Fisher Scientific - BP358-212), 0.8M NaCl, 21°C, 2% Glycerol (Fisher - G33-4) + 2 % Ethanol (Goldshield - 412804), Fluconazole 13 µM (4 mg/L, Acros Organics #455480050), Clotrimazole 6 µM (or 2 mg/L, Chem-Impex International #01311) were performed with 48 hour transfers at Stanford. .. The YPD, SC, SC with 48 hours transfer, 37°C with 280 rpm shaking, pH buffered to 7.8, and pH adjusted to 3.8

    Article Title: Antimicrobial compositions containing a synergistic combination of activated creatinine and an imidazole antifungal agent
    Article Snippet: Formulations of the invention were prepared containing clotrimazole (Chem-Impex International, Inc., Wood Dale, Ill.) and creatinine hydrochloride (Sigma-Aldrich Corporation, St. Louis, Mo.) in different molar ratios, in order to evaluate the potential effect of those ratios on antibacterial efficacy. .. T

    Concentration Assay:

    Article Title: Evolution of haploid and diploid populations reveals common, strong, and variable pleiotropic effects in non-home environments
    Article Snippet: pre-culture was used to inoculate both replicate evolutions for each condition with ~5e7 cells (~400 µL). .. Conditions with 0.2 M NaCl (Fisher Scientific – BP358-212), 0.8 M NaCl, 21°C, 2% glycerol (Fisher – G33-4)+2% ethanol (Goldshield – 412804), fluconazole 13 µM (4 mg/L, Acros Organics #455480050), Clotrimazole 6 µM (or 2 mg/L, Chem-Impex International #01311) were performed with 48 hr transfers at Stanford. .. The YPD, SC, SC with 48 hr transfer, 37°C with 280 rpm shaking, pH buffered to 7.8, and pH adjusted to 3.8 wer

    Article Title: Evolution of haploid and diploid populations reveals common, strong, and variable pleiotropic effects in non-home environments
    Article Snippet: pre- culture was used to inoculate both replicate evolutions for each condition with ~5e7 cells (~400 µL). .. Conditions with 0.2 M NaCl (Fisher Scientific – BP358- 212), 0.8 M NaCl, 21°C, 2% glycerol (Fisher – G33- 4)+2% ethanol (Goldshield – 412804), fluconazole 13 µM (4 mg/L, Acros Organics #455480050), Clotrimazole 6 µM (or 2 mg/L, Chem- Impex International #01311) were performed with 48 hr transfers Chen, Johnson, Hérissant et al. eLife 2023;12:e92899. .. DOI: https://doi.org/10.7554/eLife.92899 15 of 35 at Stanford.

    Article Title: Far From Home: Evolution of haploid and diploid populations reveals common, strong, and variable pleiotropic effects in non-home environments
    Article Snippet: pre-culture was used to inoculate both replicate evolutions for each condition with ∼5e7 cells (∼400 µL). .. Conditions with 0.2M NaCl (Fisher Scientific - BP358-212), 0.8M NaCl, 21°C, 2% Glycerol (Fisher - G33-4) + 2 % Ethanol (Goldshield - 412804), Fluconazole 13 µM (4 mg/L, Acros Organics #455480050), Clotrimazole 6 µM (or 2 mg/L, Chem-Impex International #01311) were performed with 48 hour transfers at Stanford. .. The YPD, SC, SC with 48 hours transfer, 37°C with 280 rpm shaking, pH buffered to 7.8, and pH adjusted to 3.8

    Article Title: Antimicrobial compositions containing a synergistic combination of activated creatinine and an imidazole antifungal agent
    Article Snippet: Formulations of the invention were prepared containing clotrimazole (Chem-Impex International, Inc., Wood Dale, Ill.) and creatinine hydrochloride (Sigma-Aldrich Corporation, St. Louis, Mo.) in different molar ratios, in order to evaluate the potential effect of those ratios on antibacterial efficacy. .. T

    Mutagenesis:

    Article Title: Evolution of haploid and diploid populations reveals common, strong, and variable pleiotropic effects in non-home environments
    Article Snippet: pre-culture was used to inoculate both replicate evolutions for each condition with ~5e7 cells (~400 µL). .. Conditions with 0.2 M NaCl (Fisher Scientific – BP358-212), 0.8 M NaCl, 21°C, 2% glycerol (Fisher – G33-4)+2% ethanol (Goldshield – 412804), fluconazole 13 µM (4 mg/L, Acros Organics #455480050), Clotrimazole 6 µM (or 2 mg/L, Chem-Impex International #01311) were performed with 48 hr transfers at Stanford. .. The YPD, SC, SC with 48 hr transfer, 37°C with 280 rpm shaking, pH buffered to 7.8, and pH adjusted to 3.8 wer

    Article Title: Evolution of haploid and diploid populations reveals common, strong, and variable pleiotropic effects in non-home environments
    Article Snippet: pre- culture was used to inoculate both replicate evolutions for each condition with ~5e7 cells (~400 µL). .. Conditions with 0.2 M NaCl (Fisher Scientific – BP358- 212), 0.8 M NaCl, 21°C, 2% glycerol (Fisher – G33- 4)+2% ethanol (Goldshield – 412804), fluconazole 13 µM (4 mg/L, Acros Organics #455480050), Clotrimazole 6 µM (or 2 mg/L, Chem- Impex International #01311) were performed with 48 hr transfers Chen, Johnson, Hérissant et al. eLife 2023;12:e92899. .. DOI: https://doi.org/10.7554/eLife.92899 15 of 35 at Stanford.

    Article Title: Far From Home: Evolution of haploid and diploid populations reveals common, strong, and variable pleiotropic effects in non-home environments
    Article Snippet: pre-culture was used to inoculate both replicate evolutions for each condition with ∼5e7 cells (∼400 µL). .. Conditions with 0.2M NaCl (Fisher Scientific - BP358-212), 0.8M NaCl, 21°C, 2% Glycerol (Fisher - G33-4) + 2 % Ethanol (Goldshield - 412804), Fluconazole 13 µM (4 mg/L, Acros Organics #455480050), Clotrimazole 6 µM (or 2 mg/L, Chem-Impex International #01311) were performed with 48 hour transfers at Stanford. .. The YPD, SC, SC with 48 hours transfer, 37°C with 280 rpm shaking, pH buffered to 7.8, and pH adjusted to 3.8

    Article Title: Antimicrobial compositions containing a synergistic combination of activated creatinine and an imidazole antifungal agent
    Article Snippet: Formulations of the invention were prepared containing clotrimazole (Chem-Impex International, Inc., Wood Dale, Ill.) and creatinine hydrochloride (Sigma-Aldrich Corporation, St. Louis, Mo.) in different molar ratios, in order to evaluate the potential effect of those ratios on antibacterial efficacy. .. T

    Labeling:

    Article Title: Evolution of haploid and diploid populations reveals common, strong, and variable pleiotropic effects in non-home environments
    Article Snippet: pre-culture was used to inoculate both replicate evolutions for each condition with ~5e7 cells (~400 µL). .. Conditions with 0.2 M NaCl (Fisher Scientific – BP358-212), 0.8 M NaCl, 21°C, 2% glycerol (Fisher – G33-4)+2% ethanol (Goldshield – 412804), fluconazole 13 µM (4 mg/L, Acros Organics #455480050), Clotrimazole 6 µM (or 2 mg/L, Chem-Impex International #01311) were performed with 48 hr transfers at Stanford. .. The YPD, SC, SC with 48 hr transfer, 37°C with 280 rpm shaking, pH buffered to 7.8, and pH adjusted to 3.8 wer

    Article Title: Evolution of haploid and diploid populations reveals common, strong, and variable pleiotropic effects in non-home environments
    Article Snippet: pre- culture was used to inoculate both replicate evolutions for each condition with ~5e7 cells (~400 µL). .. Conditions with 0.2 M NaCl (Fisher Scientific – BP358- 212), 0.8 M NaCl, 21°C, 2% glycerol (Fisher – G33- 4)+2% ethanol (Goldshield – 412804), fluconazole 13 µM (4 mg/L, Acros Organics #455480050), Clotrimazole 6 µM (or 2 mg/L, Chem- Impex International #01311) were performed with 48 hr transfers Chen, Johnson, Hérissant et al. eLife 2023;12:e92899. .. DOI: https://doi.org/10.7554/eLife.92899 15 of 35 at Stanford.

    Article Title: Far From Home: Evolution of haploid and diploid populations reveals common, strong, and variable pleiotropic effects in non-home environments
    Article Snippet: pre-culture was used to inoculate both replicate evolutions for each condition with ∼5e7 cells (∼400 µL). .. Conditions with 0.2M NaCl (Fisher Scientific - BP358-212), 0.8M NaCl, 21°C, 2% Glycerol (Fisher - G33-4) + 2 % Ethanol (Goldshield - 412804), Fluconazole 13 µM (4 mg/L, Acros Organics #455480050), Clotrimazole 6 µM (or 2 mg/L, Chem-Impex International #01311) were performed with 48 hour transfers at Stanford. .. The YPD, SC, SC with 48 hours transfer, 37°C with 280 rpm shaking, pH buffered to 7.8, and pH adjusted to 3.8

    Article Title: Antimicrobial compositions containing a synergistic combination of activated creatinine and an imidazole antifungal agent
    Article Snippet: Formulations of the invention were prepared containing clotrimazole (Chem-Impex International, Inc., Wood Dale, Ill.) and creatinine hydrochloride (Sigma-Aldrich Corporation, St. Louis, Mo.) in different molar ratios, in order to evaluate the potential effect of those ratios on antibacterial efficacy. .. T



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    Environmental conditions used in this study. Evolution conditions used in this study, after how many generations clones were isolated, whether adaptive mutations were identified, and abbreviations used.

    Journal: eLife

    Article Title: Evolution of haploid and diploid populations reveals common, strong, and variable pleiotropic effects in non-home environments

    doi: 10.7554/eLife.92899

    Figure Lengend Snippet: Environmental conditions used in this study. Evolution conditions used in this study, after how many generations clones were isolated, whether adaptive mutations were identified, and abbreviations used.

    Article Snippet: Conditions with 0.2 M NaCl (Fisher Scientific – BP358-212), 0.8 M NaCl, 21°C, 2% glycerol (Fisher – G33-4)+2% ethanol (Goldshield – 412804), fluconazole 13 µM (4 mg/L, Acros Organics #455480050), Clotrimazole 6 µM (or 2 mg/L, Chem-Impex International #01311) were performed with 48 hr transfers at Stanford.

    Techniques: Clone Assay, Isolation, Concentration Assay

    ( A ) Lineage tracking data of haploid bulk fitness assay (hBFA) for lineages evolved in fluconazole, clotrimazole, and glycerol/ethanol. Each column represents a replicate. The columns are grouped together by the evolution environment: fluconazole, clotrimazole, glycerol/ethanol. Each row represents the ‘test environment’. Each line represents one lineage evolved in the home environments that corresponds with its column group. The color of the line indicates the test environment in which that lineage was remeasured. ( B ) Lineage tracking data of diploid bulk fitness assay (dBFA) for lineages evolved in fluconazole, clotrimazole, and glycerol/ethanol. ( C ) Lineage tracking data of combined bulk fitness assay (cBFA) for lineages evolved in fluconazole, clotrimazole, and glycerol/ethanol.

    Journal: eLife

    Article Title: Evolution of haploid and diploid populations reveals common, strong, and variable pleiotropic effects in non-home environments

    doi: 10.7554/eLife.92899

    Figure Lengend Snippet: ( A ) Lineage tracking data of haploid bulk fitness assay (hBFA) for lineages evolved in fluconazole, clotrimazole, and glycerol/ethanol. Each column represents a replicate. The columns are grouped together by the evolution environment: fluconazole, clotrimazole, glycerol/ethanol. Each row represents the ‘test environment’. Each line represents one lineage evolved in the home environments that corresponds with its column group. The color of the line indicates the test environment in which that lineage was remeasured. ( B ) Lineage tracking data of diploid bulk fitness assay (dBFA) for lineages evolved in fluconazole, clotrimazole, and glycerol/ethanol. ( C ) Lineage tracking data of combined bulk fitness assay (cBFA) for lineages evolved in fluconazole, clotrimazole, and glycerol/ethanol.

    Article Snippet: Conditions with 0.2 M NaCl (Fisher Scientific – BP358-212), 0.8 M NaCl, 21°C, 2% glycerol (Fisher – G33-4)+2% ethanol (Goldshield – 412804), fluconazole 13 µM (4 mg/L, Acros Organics #455480050), Clotrimazole 6 µM (or 2 mg/L, Chem-Impex International #01311) were performed with 48 hr transfers at Stanford.

    Techniques:

    These heatmaps show the fitnesses of all lineages including lineages that had the same mutation and were collapsed into a single row using the median fitnesses in . The number at the end of the name represents numerical barcode identification number. ( A ) Mutations identified in clotrimazole 1N measured in haploid bulk fitness assay (hBFA). ( B ) Mutations identified in clotrimazole 2N measured in diploid bulk fitness assay (dBFA). ( C ) Mutations identified in fluconazole 2N measured in combined bulk fitness assay (cBFA). ( D ) Mutations identified in fluconazole 2N measured in dBFA. ( E ) Mutations identified in glycerol/ethanol 1N measured in hBFA. ( F ) Mutations identified in glycerol/ethanol 2N measured in cBFA.

    Journal: eLife

    Article Title: Evolution of haploid and diploid populations reveals common, strong, and variable pleiotropic effects in non-home environments

    doi: 10.7554/eLife.92899

    Figure Lengend Snippet: These heatmaps show the fitnesses of all lineages including lineages that had the same mutation and were collapsed into a single row using the median fitnesses in . The number at the end of the name represents numerical barcode identification number. ( A ) Mutations identified in clotrimazole 1N measured in haploid bulk fitness assay (hBFA). ( B ) Mutations identified in clotrimazole 2N measured in diploid bulk fitness assay (dBFA). ( C ) Mutations identified in fluconazole 2N measured in combined bulk fitness assay (cBFA). ( D ) Mutations identified in fluconazole 2N measured in dBFA. ( E ) Mutations identified in glycerol/ethanol 1N measured in hBFA. ( F ) Mutations identified in glycerol/ethanol 2N measured in cBFA.

    Article Snippet: Conditions with 0.2 M NaCl (Fisher Scientific – BP358-212), 0.8 M NaCl, 21°C, 2% glycerol (Fisher – G33-4)+2% ethanol (Goldshield – 412804), fluconazole 13 µM (4 mg/L, Acros Organics #455480050), Clotrimazole 6 µM (or 2 mg/L, Chem-Impex International #01311) were performed with 48 hr transfers at Stanford.

    Techniques: Mutagenesis

    The columns are the home environments that lineages evolved in and the rows are the test environments in which their fitnesses were remeasured. X axis is the fitness of the lineages remeasured in the bulk fitness assay (BFA) in their home environment. Y axis is the fitness of the lineages in a non-home environment. No fitness remeasurements are available from BFAs grown in clotrimazole.

    Journal: eLife

    Article Title: Evolution of haploid and diploid populations reveals common, strong, and variable pleiotropic effects in non-home environments

    doi: 10.7554/eLife.92899

    Figure Lengend Snippet: The columns are the home environments that lineages evolved in and the rows are the test environments in which their fitnesses were remeasured. X axis is the fitness of the lineages remeasured in the bulk fitness assay (BFA) in their home environment. Y axis is the fitness of the lineages in a non-home environment. No fitness remeasurements are available from BFAs grown in clotrimazole.

    Article Snippet: Conditions with 0.2 M NaCl (Fisher Scientific – BP358-212), 0.8 M NaCl, 21°C, 2% glycerol (Fisher – G33-4)+2% ethanol (Goldshield – 412804), fluconazole 13 µM (4 mg/L, Acros Organics #455480050), Clotrimazole 6 µM (or 2 mg/L, Chem-Impex International #01311) were performed with 48 hr transfers at Stanford.

    Techniques:

    The distribution of fitness effects for all adaptive diploid lineages remeasured in clotrimazole, fluconazole, and glycerol/ethanol. The gray vertical line delineates the fitness threshold of the top 10% of mutants that were evolved in the labeled environment. The red line delineated the top fitness of all mutants that were evolved in the labeled environment. The y axis delineates the percentage of mutants that have a specific fitness (x axis).

    Journal: eLife

    Article Title: Evolution of haploid and diploid populations reveals common, strong, and variable pleiotropic effects in non-home environments

    doi: 10.7554/eLife.92899

    Figure Lengend Snippet: The distribution of fitness effects for all adaptive diploid lineages remeasured in clotrimazole, fluconazole, and glycerol/ethanol. The gray vertical line delineates the fitness threshold of the top 10% of mutants that were evolved in the labeled environment. The red line delineated the top fitness of all mutants that were evolved in the labeled environment. The y axis delineates the percentage of mutants that have a specific fitness (x axis).

    Article Snippet: Conditions with 0.2 M NaCl (Fisher Scientific – BP358-212), 0.8 M NaCl, 21°C, 2% glycerol (Fisher – G33-4)+2% ethanol (Goldshield – 412804), fluconazole 13 µM (4 mg/L, Acros Organics #455480050), Clotrimazole 6 µM (or 2 mg/L, Chem-Impex International #01311) were performed with 48 hr transfers at Stanford.

    Techniques: Labeling