bicuculline methobromide bcc (Tocris)
Tocris is a verified supplier 
Tocris manufactures this product 
93
Structured Review
Tocris
bicuculline methobromide bcc
Bicuculline Methobromide Bcc, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0109/(-)-Bicuculline+methobromide/bio_rxiv__2021__05__21__445170-43-9-12
Average 93 stars, based on 117 article reviews
Bicuculline Methobromide Bcc, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0109/(-)-Bicuculline+methobromide/bio_rxiv__2021__05__21__445170-43-9-12
Average 93 stars, based on 117 article reviews
bicuculline methobromide bcc - by Bioz Stars,
2026-09
93/100 stars
Images
1) Product Images from "Coordinated regulation of CB1 cannabinoid receptors and anandamide metabolism stabilize network activity during homeostatic scaling down"
Article Title: Coordinated regulation of CB1 cannabinoid receptors and anandamide metabolism stabilize network activity during homeostatic scaling down
Journal: bioRxiv
doi: 10.1101/2021.05.21.445170
Figure Legend Snippet: Time course of homeostatic down-scaling in rat primary cortical neuron culture. (A,B) Representative western blots and quantification of total protein expression in DIV17 neurons treated by control media for 48h (Ctrl 48h) or by 20μM bicuculline (BCC) for 4h, 12h, 24h or 48h. FAAH is significantly downregulated from 12h and onward, but CB1 do not become significantly upregulated until the end of the 48h time course. Results are normalized for each protein to its expression levels in vehicle 48h (dotted line), and presented as mean ± SEM from four to six independent culture preparations with duplicate wells. (C) Quantification of targeted lipidomics analyzing 2-AG, AEA and various n-acylethanolamines (NAEs) in DIV17 neurons untreated or treated by 20μM BCC for 4h, 24h or 48h. Note that the neurons transition from a 2-AG dominated response at 4h to an AEA- and NAEs-dominated response at 24h and 48h, corresponding with the downregulation of FAAH shown in (B) . Results from each treatment group are normalized to a matching control, and presented as mean ± SEM from three to four independent culture preparations with duplicate plates. One-way ANOVA with Dunnett’s multiple comparison test *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.
Techniques Used: Western Blot, Expressing, Control, Comparison
Figure Legend Snippet: Glutamatergic and cannabinergic adaptations occur through independent mechanisms. (A,B) Representative blots and quantifications of surface and total protein expression in DIV21-25 neurons treated by DMSO or 20μM BCC in the absence or presence of 1μM MTEP hydrochloride, a selective mGluR5 antagonist, and 100nM JNJ 16259685, a selective mGluR1 antagonist (MTEP/JNJ). MTEP/JNJ blocked BCC-induced downregulation of surface GluA1 but not the upregulation of surface CB1. (C,D) Representative blots and quantifications of surface and total protein expression in DIV21-25 neurons treated by DMSO or 20μM bicuculline (BCC) in the absence or presence of 500nM AM251, a selective CB1 antagonist. AM251 did not block BCC-induced downregulation of surface GluA1 or the upregulation of surface CB1. Results are normalized for each protein to its expression levels in control group, and presented as mean ± SEM from four to six independent culture preparations with triplicate wells. One-way ANOVA with Šidák’s multiple comparison test *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.
Techniques Used: Expressing, Blocking Assay, Control, Comparison
Figure Legend Snippet: Acute application of AM251 blocks synchronous up-state like glutamatatergic events (A) Representative traces of GluSnFR time-lapse recorded in DIV21-25 neurons untreated (Ctrl) or treated with bicuculline for 48hr (BCC 48hr) followed by acute application (10-40min) of AM251 (Acute AM251 and BCC 48hr + Acute AM251). Results are presented as fluorescence for each recording normalized to its minimum fluorescence (F/F min , dotted line). Scale bar represents 10s (horizontal) and 10% change from minimum fluorescence (vertical). (B) Quantification of interevent intervals (left) and area under curve (right) of data shown in (A) . AM251 causes synchronous glutamate events to become fragmented, even in the presence of BCC. Results are presented as mean ± SEM from three to six independent culture preparations with four to eight wells in each culture. One-way ANOVA with Šidák’s multiple comparison test. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.
Techniques Used: Fluorescence, Comparison
Figure Legend Snippet: Detection of synchronous and asynchronous release of glutamate by iGluSNFR in mature (DIV21-25) neurons (A) Double derivative images showing spontaneous changes in GluSnFR fluorescence corresponding to a single synchronous glutamate event under control condition. (B) Double derivative images showing spontaneous changes in iGluSNFR fluorescence corresponding to multiple asynchronous glutamate events. Localized events are indicated by yellow arrows. (C-F) Representative traces of iGluSNFR time-lapse recorded in DIV21-25 cultured cortical neurons untreated (Ctrl, C ) or treated with acute application of 50μM D-AP5 (Acute D-AP5, D ), 1μM TTX (Acute TTX, E ) or 20μM bicuculline (Acute BCC, F ). Mutiple example traces are shown each representing a separate transfected neuron. While acute BCC causes a plateau in the synchronous events, acute D-AP5 leads to dramatically reduced amplitude of synchronous events and TTX completely abolishes them. Results are presented as fluorescence for each recording normalized to its minimum fluorescence (F/F min , dotted line). Scale bar represents 10s (horizontal) and 10% change from minimum fluorescence (vertical). Ctrl data in panel (C) is from the same set of data as in Figure 5 .
Techniques Used: Fluorescence, Control, Cell Culture, Transfection
Figure Legend Snippet: CB1 expression is upregulated during homeostatic downscaling in mature neurons. (A) Representative western blots of surface and total protein expression in developing (DIV11-12) and mature (DIV21-25) cultured cortical neurons treated for 48h with control media (Ctrl), 20μM bicuculline (BCC) or 1μM tetrodotoxin (TTX). (B,C) Quantification of data shown in (A) in developing neurons (B) and mature neurons (C) . BCC and TTX treated samples are normalized to control treatment. BCC induced downregulation surface GluA1 and S845 dephosphorylation regardless of age, and upregulation of total and surface CB1 only in mature neurons. (D,E) Representative western blots and quantification of total protein expression in cultured cortical neurons at DIV8, DIV15 and DIV22. Results are normalized for each protein to its expression levels at DIV15, and presented as mean ± SEM from at least three independent culture preparations with quadruplicate wells. Standard unpaired t-test was used for (B,C) and one-way ANOVA with Dunnett’s multiple comparison test was used for (E) . *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001
Techniques Used: Expressing, Western Blot, Cell Culture, Control, De-Phosphorylation Assay, Comparison
Related Articles
Electron Microscopy:Article Title: BEST1-mediated tonic excitation facilitates anxiety-like behaviors in a mouse model of chronic neuropathic pain. Article Snippet: neChem NA AAV-Syn-taCasp3-eGFP BrainVTA Cat# PT-2433 AAV-Syn-eGFP BrainVTA Cat# PT-1990 Chemicals, peptides, and recombinant proteins Isoflurane RWD Life Science Cat# R510-220 Dihydrokainic acid (DHK) Microscopy:Article Title: BEST1-mediated tonic excitation facilitates anxiety-like behaviors in a mouse model of chronic neuropathic pain. Article Snippet: neChem NA AAV-Syn-taCasp3-eGFP BrainVTA Cat# PT-2433 AAV-Syn-eGFP BrainVTA Cat# PT-1990 Chemicals, peptides, and recombinant proteins Isoflurane RWD Life Science Cat# R510-220 Dihydrokainic acid (DHK) |
