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resource source identifier recombinant mouse leukemia inhibitory factor mlif amsbio  (AMS Biotechnology)


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    AMS Biotechnology resource source identifier recombinant mouse leukemia inhibitory factor mlif amsbio
    Resource Source Identifier Recombinant Mouse Leukemia Inhibitory Factor Mlif Amsbio, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/01-a1140-0050/Recombinant+mouse+leukemia+inhibitory+factor+mLIF+%2F+LIF+-+Animal+Free/pm34358448-245-2-11
    Average 96 stars, based on 6 article reviews
    resource source identifier recombinant mouse leukemia inhibitory factor mlif amsbio - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Knock-Out:

    Article Title: Genetic Suppression of Cryoprotectant Toxicity
    Article Snippet: .. Embryonic stem cell (ESC) medium is KnockOut Dulbecco’s Modified Eagle’s Medium (Gibco, product 10829–018) supplemented with 15% fetal bovine serum (Tissue Culture Biologicals, product 104), GlutaMAX (2 mM L-alanyl-L-glutamine dipeptide in 0.85% NaCl; Gibco product 35050061), 0.1 mM non-essential amino acids (Gibco, product 1140–050), penicillin-streptomycin (25 units/ml, Thermo Fisher, product 15140122), beta-mercaptoethanol (55 μM, Thermo Fisher, product 21985–023) and 1,000 unit per ml of leukemia inhibitory factor (AMSBio, product AMS-263–100). .. DPBS is Dulbecco’s phosphate-buffered saline supplemented with calcium and magnesium (Gibco, product 14040–133).

    Article Title: A Modular Assembly Platform for Rapid Generation of DNA Constructs.
    Article Snippet: Human embryonic kidney cells (HEK-293FS), 3TB cells, and KP cells were cultured in DMEM (Corning) supplemented with L-glutamine (2 mM), penicillin (100 U/mL), streptomyocin (100 μ g/ mL), and 10% fetal calf serum (FCS; Gibco) at 37 °C in a 5% CO2 humidified atmosphere. .. Embryonic stem (ES) cells were cultured on primary irradiated mouse embryonic fibroblasts in Knockout DMEM (Life Technologies) supplemented with β -mercaptoethanol (100 μ M), L-glutamine (2 mM), penicillin (100 U/mL), streptomycin (100 μ g/mL), 1X Non-essential Amino Acids (Sigma), leukemia inhibitory factor (200 pg/mL; Amsbio), CHIR99021 (3 μ M; P212121) and PD0325901 (1 μ M; Selleck Chemicals), and 15% FCS at 37° in a 5% CO2 humidified atmosphere. .. Following transduction of 3TB or KP cells with lentivirus or retrovirus, 5 μ g/mL puromycin (Life Technologies), 400 μ g/mL hygromycin (EMD Millipore), 400 μ g/mL zeocin (Life Technologies), or 20 μ g/mL blasticidin (Invitrogen) selection was applied, as appropriate.

    Modification:

    Article Title: Genetic Suppression of Cryoprotectant Toxicity
    Article Snippet: .. Embryonic stem cell (ESC) medium is KnockOut Dulbecco’s Modified Eagle’s Medium (Gibco, product 10829–018) supplemented with 15% fetal bovine serum (Tissue Culture Biologicals, product 104), GlutaMAX (2 mM L-alanyl-L-glutamine dipeptide in 0.85% NaCl; Gibco product 35050061), 0.1 mM non-essential amino acids (Gibco, product 1140–050), penicillin-streptomycin (25 units/ml, Thermo Fisher, product 15140122), beta-mercaptoethanol (55 μM, Thermo Fisher, product 21985–023) and 1,000 unit per ml of leukemia inhibitory factor (AMSBio, product AMS-263–100). .. DPBS is Dulbecco’s phosphate-buffered saline supplemented with calcium and magnesium (Gibco, product 14040–133).

    Affinity Magnetic Separation:

    Article Title: Genetic Suppression of Cryoprotectant Toxicity
    Article Snippet: .. Embryonic stem cell (ESC) medium is KnockOut Dulbecco’s Modified Eagle’s Medium (Gibco, product 10829–018) supplemented with 15% fetal bovine serum (Tissue Culture Biologicals, product 104), GlutaMAX (2 mM L-alanyl-L-glutamine dipeptide in 0.85% NaCl; Gibco product 35050061), 0.1 mM non-essential amino acids (Gibco, product 1140–050), penicillin-streptomycin (25 units/ml, Thermo Fisher, product 15140122), beta-mercaptoethanol (55 μM, Thermo Fisher, product 21985–023) and 1,000 unit per ml of leukemia inhibitory factor (AMSBio, product AMS-263–100). .. DPBS is Dulbecco’s phosphate-buffered saline supplemented with calcium and magnesium (Gibco, product 14040–133).

    Article Title: The CD155/TIGIT axis promotes and maintains immune evasion in neoantigen-expressing pancreatic cancer.
    Article Snippet: In brief Freed-Pastor et al. identify the CD155/ TIGIT axis as a key driver of immune evasion in pancreas cancer.. Neoepitope prediction reveals a subset of human pancreas cancer patients with predicted high-affinity neoepitopes and functional interrogation using preclinical models identifies a combination immunotherapy approach (TIGIT/PD-1 co-blockade plus CD40 agonism) capable of eliciting profound anti-tumor responses.

    Article Title: Light-sheet microscopy of network activity in 3D neuronal systems
    Article Snippet: Mouse embryonic stem cell line (kind gift from Yung Su Justin Kim from Dr. Ivan Bedzhov, MPI Münster) were maintained under 5% CO 2 and 37°C conditions on 10 % gelatine-coated (Sigma, G1393) T25 flasks in DMEM high glucose medium (Sigma D5671) containing 15% FBS (Sigma S0615), 100 U/ml Penicillin-Streptomycin, 2 mM L-Glutamine, 1 mM sodium pyruvate (Sigma, S8636), 0.1 mM NEAA (Sigma, M7145), mM 2-mercaptoethanol (Sigma, M3148). .. Maintenance medium was freshly supplemented with 0.44 nM mLif (Amsbio, AMS-263) 0.4 μ M PD 0325901 and 3 μ M CHIR 99021 inhibitors (Cayman Chemicals). ..

    Cell Culture:

    Article Title: A Modular Assembly Platform for Rapid Generation of DNA Constructs.
    Article Snippet: Human embryonic kidney cells (HEK-293FS), 3TB cells, and KP cells were cultured in DMEM (Corning) supplemented with L-glutamine (2 mM), penicillin (100 U/mL), streptomyocin (100 μ g/ mL), and 10% fetal calf serum (FCS; Gibco) at 37 °C in a 5% CO2 humidified atmosphere. .. Embryonic stem (ES) cells were cultured on primary irradiated mouse embryonic fibroblasts in Knockout DMEM (Life Technologies) supplemented with β -mercaptoethanol (100 μ M), L-glutamine (2 mM), penicillin (100 U/mL), streptomycin (100 μ g/mL), 1X Non-essential Amino Acids (Sigma), leukemia inhibitory factor (200 pg/mL; Amsbio), CHIR99021 (3 μ M; P212121) and PD0325901 (1 μ M; Selleck Chemicals), and 15% FCS at 37° in a 5% CO2 humidified atmosphere. .. Following transduction of 3TB or KP cells with lentivirus or retrovirus, 5 μ g/mL puromycin (Life Technologies), 400 μ g/mL hygromycin (EMD Millipore), 400 μ g/mL zeocin (Life Technologies), or 20 μ g/mL blasticidin (Invitrogen) selection was applied, as appropriate.

    Article Title: Impact of MicroRNA Levels, Target-Site Complementarity, and Cooperativity on Competing Endogenous RNA-Regulated Gene Expression
    Article Snippet: .. The male embryonic stem cell line E14 (from Prof. Constance Ciaudo laboratory) derived from 129/Ola mice was cultured in ESC media [DMEM 4.5 g/l glucose (Life Technologies), containing 15% heat-inactivated fetal bovine serum (Life Technologies), 1000 U/ml recombinant mouse LIF (Amsbio), 0.1 mM 2- mercaptoethanol (Sigma), 1% Penicillin-Streptomycin (Life Technologies), 1% Glutamax (Life Technologies), and 1mM sodium pyruvate (Life Technologies)] on a 0.1% gelatin-coated plated in the absence of feeder cells. .. For transfection, plates with ~80% confluence were trypsinized and plated at 2 x 105 cells/well in gelatincoated Nucleon Delta Surface 12-well plates (Thermo Scientific).

    Irradiation:

    Article Title: A Modular Assembly Platform for Rapid Generation of DNA Constructs.
    Article Snippet: Human embryonic kidney cells (HEK-293FS), 3TB cells, and KP cells were cultured in DMEM (Corning) supplemented with L-glutamine (2 mM), penicillin (100 U/mL), streptomyocin (100 μ g/ mL), and 10% fetal calf serum (FCS; Gibco) at 37 °C in a 5% CO2 humidified atmosphere. .. Embryonic stem (ES) cells were cultured on primary irradiated mouse embryonic fibroblasts in Knockout DMEM (Life Technologies) supplemented with β -mercaptoethanol (100 μ M), L-glutamine (2 mM), penicillin (100 U/mL), streptomycin (100 μ g/mL), 1X Non-essential Amino Acids (Sigma), leukemia inhibitory factor (200 pg/mL; Amsbio), CHIR99021 (3 μ M; P212121) and PD0325901 (1 μ M; Selleck Chemicals), and 15% FCS at 37° in a 5% CO2 humidified atmosphere. .. Following transduction of 3TB or KP cells with lentivirus or retrovirus, 5 μ g/mL puromycin (Life Technologies), 400 μ g/mL hygromycin (EMD Millipore), 400 μ g/mL zeocin (Life Technologies), or 20 μ g/mL blasticidin (Invitrogen) selection was applied, as appropriate.

    Recombinant:

    Article Title: The CD155/TIGIT axis promotes and maintains immune evasion in neoantigen-expressing pancreatic cancer.
    Article Snippet: In brief Freed-Pastor et al. identify the CD155/ TIGIT axis as a key driver of immune evasion in pancreas cancer.. Neoepitope prediction reveals a subset of human pancreas cancer patients with predicted high-affinity neoepitopes and functional interrogation using preclinical models identifies a combination immunotherapy approach (TIGIT/PD-1 co-blockade plus CD40 agonism) capable of eliciting profound anti-tumor responses.

    Article Title: Impact of MicroRNA Levels, Target-Site Complementarity, and Cooperativity on Competing Endogenous RNA-Regulated Gene Expression
    Article Snippet: .. The male embryonic stem cell line E14 (from Prof. Constance Ciaudo laboratory) derived from 129/Ola mice was cultured in ESC media [DMEM 4.5 g/l glucose (Life Technologies), containing 15% heat-inactivated fetal bovine serum (Life Technologies), 1000 U/ml recombinant mouse LIF (Amsbio), 0.1 mM 2- mercaptoethanol (Sigma), 1% Penicillin-Streptomycin (Life Technologies), 1% Glutamax (Life Technologies), and 1mM sodium pyruvate (Life Technologies)] on a 0.1% gelatin-coated plated in the absence of feeder cells. .. For transfection, plates with ~80% confluence were trypsinized and plated at 2 x 105 cells/well in gelatincoated Nucleon Delta Surface 12-well plates (Thermo Scientific).

    Membrane:

    Article Title: The CD155/TIGIT axis promotes and maintains immune evasion in neoantigen-expressing pancreatic cancer.
    Article Snippet: In brief Freed-Pastor et al. identify the CD155/ TIGIT axis as a key driver of immune evasion in pancreas cancer.. Neoepitope prediction reveals a subset of human pancreas cancer patients with predicted high-affinity neoepitopes and functional interrogation using preclinical models identifies a combination immunotherapy approach (TIGIT/PD-1 co-blockade plus CD40 agonism) capable of eliciting profound anti-tumor responses.

    Activation Assay:

    Article Title: The CD155/TIGIT axis promotes and maintains immune evasion in neoantigen-expressing pancreatic cancer.
    Article Snippet: In brief Freed-Pastor et al. identify the CD155/ TIGIT axis as a key driver of immune evasion in pancreas cancer.. Neoepitope prediction reveals a subset of human pancreas cancer patients with predicted high-affinity neoepitopes and functional interrogation using preclinical models identifies a combination immunotherapy approach (TIGIT/PD-1 co-blockade plus CD40 agonism) capable of eliciting profound anti-tumor responses.

    Formalin-fixed Paraffin-Embedded:

    Article Title: The CD155/TIGIT axis promotes and maintains immune evasion in neoantigen-expressing pancreatic cancer.
    Article Snippet: In brief Freed-Pastor et al. identify the CD155/ TIGIT axis as a key driver of immune evasion in pancreas cancer.. Neoepitope prediction reveals a subset of human pancreas cancer patients with predicted high-affinity neoepitopes and functional interrogation using preclinical models identifies a combination immunotherapy approach (TIGIT/PD-1 co-blockade plus CD40 agonism) capable of eliciting profound anti-tumor responses.

    Hybridization:

    Article Title: The CD155/TIGIT axis promotes and maintains immune evasion in neoantigen-expressing pancreatic cancer.
    Article Snippet: In brief Freed-Pastor et al. identify the CD155/ TIGIT axis as a key driver of immune evasion in pancreas cancer.. Neoepitope prediction reveals a subset of human pancreas cancer patients with predicted high-affinity neoepitopes and functional interrogation using preclinical models identifies a combination immunotherapy approach (TIGIT/PD-1 co-blockade plus CD40 agonism) capable of eliciting profound anti-tumor responses.

    Sequencing:

    Article Title: The CD155/TIGIT axis promotes and maintains immune evasion in neoantigen-expressing pancreatic cancer.
    Article Snippet: In brief Freed-Pastor et al. identify the CD155/ TIGIT axis as a key driver of immune evasion in pancreas cancer.. Neoepitope prediction reveals a subset of human pancreas cancer patients with predicted high-affinity neoepitopes and functional interrogation using preclinical models identifies a combination immunotherapy approach (TIGIT/PD-1 co-blockade plus CD40 agonism) capable of eliciting profound anti-tumor responses.

    RNA Sequencing:

    Article Title: The CD155/TIGIT axis promotes and maintains immune evasion in neoantigen-expressing pancreatic cancer.
    Article Snippet: In brief Freed-Pastor et al. identify the CD155/ TIGIT axis as a key driver of immune evasion in pancreas cancer.. Neoepitope prediction reveals a subset of human pancreas cancer patients with predicted high-affinity neoepitopes and functional interrogation using preclinical models identifies a combination immunotherapy approach (TIGIT/PD-1 co-blockade plus CD40 agonism) capable of eliciting profound anti-tumor responses.

    Derivative Assay:

    Article Title: Impact of MicroRNA Levels, Target-Site Complementarity, and Cooperativity on Competing Endogenous RNA-Regulated Gene Expression
    Article Snippet: .. The male embryonic stem cell line E14 (from Prof. Constance Ciaudo laboratory) derived from 129/Ola mice was cultured in ESC media [DMEM 4.5 g/l glucose (Life Technologies), containing 15% heat-inactivated fetal bovine serum (Life Technologies), 1000 U/ml recombinant mouse LIF (Amsbio), 0.1 mM 2- mercaptoethanol (Sigma), 1% Penicillin-Streptomycin (Life Technologies), 1% Glutamax (Life Technologies), and 1mM sodium pyruvate (Life Technologies)] on a 0.1% gelatin-coated plated in the absence of feeder cells. .. For transfection, plates with ~80% confluence were trypsinized and plated at 2 x 105 cells/well in gelatincoated Nucleon Delta Surface 12-well plates (Thermo Scientific).

    other:

    Article Title: Impact of MicroRNA Levels, Target-Site Complementarity, and Cooperativity on Competing Endogenous RNA-Regulated Gene Expression
    Article Snippet: For transfection, plates with ~80% confluence were trypsinized and plated at 2 x 105 cells/well in gelatin- coated Nucleon Delta Surface 12-well plates (Thermo Scientific).



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