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rabbit anti ne  (Athens Research)


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    Structured Review

    Athens Research rabbit anti ne
    Rabbit Anti Ne, supplied by Athens Research, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/01-14-051200/Anti-Elastase%2C+Polyclonal+Ab+produced+in+rabbit/pm36732772-93-21-23
    Average 94 stars, based on 9 article reviews
    rabbit anti ne - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Staining:

    Article Title: Neutrophil functions in patients with neutropenia due to glycogen storage disease type 1b treated with empagliflozin
    Article Snippet: .. Cells were stained with a rabbit anti-human neutrophil elastase antibody (no. 01-14-051200, Athens Research and Technology) diluted at 1:500 in phosphate-buffered saline containing 3% bovine serum albumin and 0.1% saponin for 1 hour, followed by APC-conjugated goat anti-rabbit immunoglobulin G F(ab′) 2 (no. 111-136-144, Jackson ImmunoResearch Laboratories) diluted at 1:1000 for 45 minutes. .. Nuclei were counterstained with 1 μg/mL Hoechst 33342 (Invitrogen).

    Article Title: Neutrophil functions in patients with neutropenia due to glycogen storage disease type 1b treated with empagliflozin.
    Article Snippet: The quantity of DNA was determined using SYTOX Green Nucleic Acid Stain (Thermo Fisher Scientific) at an excitation wavelength of 485 nm and an emission wavelength of 535 nm. .. NETs were also identified by confocal microscopy, as previously described.21 Cells were stained with a rabbit anti-human neutrophil elastase antibody (no. 01-14-051200, Athens Research and Technology) diluted at 1:500 in phosphatebuffered saline containing 3% bovine serum albumin and 0.1% saponin for 1 hour, followed by APC-conjugated goat anti-rabbit immunoglobulin G F(ab′)2 (no. 111-136-144, Jackson ImmunoResearch Laboratories) diluted at 1:1000 for 45 minutes. .. Nuclei were counterstained with 1 μg/mL Hoechst 33342 (Invitrogen).

    Saline:

    Article Title: Neutrophil functions in patients with neutropenia due to glycogen storage disease type 1b treated with empagliflozin
    Article Snippet: .. Cells were stained with a rabbit anti-human neutrophil elastase antibody (no. 01-14-051200, Athens Research and Technology) diluted at 1:500 in phosphate-buffered saline containing 3% bovine serum albumin and 0.1% saponin for 1 hour, followed by APC-conjugated goat anti-rabbit immunoglobulin G F(ab′) 2 (no. 111-136-144, Jackson ImmunoResearch Laboratories) diluted at 1:1000 for 45 minutes. .. Nuclei were counterstained with 1 μg/mL Hoechst 33342 (Invitrogen).

    Article Title: Neutrophil functions in patients with neutropenia due to glycogen storage disease type 1b treated with empagliflozin.
    Article Snippet: The quantity of DNA was determined using SYTOX Green Nucleic Acid Stain (Thermo Fisher Scientific) at an excitation wavelength of 485 nm and an emission wavelength of 535 nm. .. NETs were also identified by confocal microscopy, as previously described.21 Cells were stained with a rabbit anti-human neutrophil elastase antibody (no. 01-14-051200, Athens Research and Technology) diluted at 1:500 in phosphatebuffered saline containing 3% bovine serum albumin and 0.1% saponin for 1 hour, followed by APC-conjugated goat anti-rabbit immunoglobulin G F(ab′)2 (no. 111-136-144, Jackson ImmunoResearch Laboratories) diluted at 1:1000 for 45 minutes. .. Nuclei were counterstained with 1 μg/mL Hoechst 33342 (Invitrogen).

    Incubation:

    Article Title: Probing of the reactive center loop region of alpha-1-antitrypsin by mutagenesis predicts new type-2 dysfunctional variants
    Article Snippet: .. After three rounds of washing with PBS-T, detection of NE was carried out by incubation with 200 ng/mL rabbit polyclonal antibody anti-NE (Athens Research & Technology, 01-14-051200) in BSA-containing dilution buffer, for 1 h at 37 °C. .. HRP-conjugated donkey antibody anti-rabbit IgG (Cytiva, NA9340) was applied at a concentration of 67 ng/mL in dilution buffer.

    Article Title: Lung Inflammation in alpha-1-antitrypsin deficient individuals with normal lung function.
    Article Snippet: Samples and standards pre-treated with 1 mM PMSF (Sigma, St. Louis, MO. .. Cat # P7626) were loaded to the sheep anti-human NE coated plates and were incubated at 37 °C for 1 h. Rabbit anti-NE (Athens Research and Technology, Athens, GA. Cat # 01-14-051200) was added and incubated for 1 h 37 °C. ..

    Article Title: Probing of the reactive center loop region of alpha-1-antitrypsin by mutagenesis predicts new type-2 dysfunctional variants.
    Article Snippet: Lung disease in alpha-1-antitrypsin deficiency (AATD) mainly results from insufficient control of the serine proteases neutrophil elastase (NE) and proteinase-3 due to reduced plasma levels of alpha-1-antitrypsin (AAT) variants.. Mutations in the specificity-determining reactive center loop (RCL) of AAT would be predicted to minimally affect protein folding and secretion by hepatocytes but can impair anti-protease activity or alter the target protease.. These properly secreted but dysfunctional ‘type-2’ variants would not be identified by common diagnostic protocols that are predicated on a reduction in circulating AAT.

    Western Blot:

    Article Title: Neutrophil Elastase Limits Spread of Staphylococcus aureus during Skin Infection
    Article Snippet: .. The presence of NE protein was assessed in neutrophil lysates through western blot with rabbit anti-mouse NE polyclonal IgG (ab68672; Abcam), or in whole fixed cells through fluorescent microscopy with rabbit anti-NE polyclonal IgG (01-14-051200; Athens Research and Technology), each followed by appropriate secondary antibodies ( ). ..

    Article Title: Neutrophil elastase limits spread of Staphylococcus aureus during skin infection.
    Article Snippet: Neutrophil elastase (NE), the main protease of neutrophils, is involved in many of their functions, including prevention of infections.. Despite its general importance, NE was previously thought to play no role in infections caused by Staphylococcus aureus, one of the leading bacterial pathogens.. Here, using transgenic mice deficient in NE and a mouse model of localized skin infection, we demonstrate that NE is in fact needed for the containment of S. aureus and prevention of the systemic spread of this bacterium.

    Microscopy:

    Article Title: Neutrophil Elastase Limits Spread of Staphylococcus aureus during Skin Infection
    Article Snippet: .. The presence of NE protein was assessed in neutrophil lysates through western blot with rabbit anti-mouse NE polyclonal IgG (ab68672; Abcam), or in whole fixed cells through fluorescent microscopy with rabbit anti-NE polyclonal IgG (01-14-051200; Athens Research and Technology), each followed by appropriate secondary antibodies ( ). ..

    Article Title: Neutrophil elastase limits spread of Staphylococcus aureus during skin infection.
    Article Snippet: Neutrophil elastase (NE), the main protease of neutrophils, is involved in many of their functions, including prevention of infections.. Despite its general importance, NE was previously thought to play no role in infections caused by Staphylococcus aureus, one of the leading bacterial pathogens.. Here, using transgenic mice deficient in NE and a mouse model of localized skin infection, we demonstrate that NE is in fact needed for the containment of S. aureus and prevention of the systemic spread of this bacterium.

    Confocal Microscopy:

    Article Title: Neutrophil functions in patients with neutropenia due to glycogen storage disease type 1b treated with empagliflozin.
    Article Snippet: The quantity of DNA was determined using SYTOX Green Nucleic Acid Stain (Thermo Fisher Scientific) at an excitation wavelength of 485 nm and an emission wavelength of 535 nm. .. NETs were also identified by confocal microscopy, as previously described.21 Cells were stained with a rabbit anti-human neutrophil elastase antibody (no. 01-14-051200, Athens Research and Technology) diluted at 1:500 in phosphatebuffered saline containing 3% bovine serum albumin and 0.1% saponin for 1 hour, followed by APC-conjugated goat anti-rabbit immunoglobulin G F(ab′)2 (no. 111-136-144, Jackson ImmunoResearch Laboratories) diluted at 1:1000 for 45 minutes. .. Nuclei were counterstained with 1 μg/mL Hoechst 33342 (Invitrogen).



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    Figure 4. Effect of empagliflozin on phagocytosis and NETosis. (A-C) The phagocytic activity of freshly isolated <t>neutrophils</t> was determined in the 7 patients with GSD1b
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    Figure 4. Effect of empagliflozin on phagocytosis and NETosis. (A-C) The phagocytic activity of freshly isolated <t>neutrophils</t> was determined in the 7 patients with GSD1b
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    Athens Research polyclonal rabbit-anti-ne igg
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    Image Search Results


    Figure 4. Effect of empagliflozin on phagocytosis and NETosis. (A-C) The phagocytic activity of freshly isolated neutrophils was determined in the 7 patients with GSD1b

    Journal: Blood advances

    Article Title: Neutrophil functions in patients with neutropenia due to glycogen storage disease type 1b treated with empagliflozin.

    doi: 10.1182/bloodadvances.2023012403

    Figure Lengend Snippet: Figure 4. Effect of empagliflozin on phagocytosis and NETosis. (A-C) The phagocytic activity of freshly isolated neutrophils was determined in the 7 patients with GSD1b

    Article Snippet: NETs were also identified by confocal microscopy, as previously described.21 Cells were stained with a rabbit anti-human neutrophil elastase antibody (no. 01-14-051200, Athens Research and Technology) diluted at 1:500 in phosphatebuffered saline containing 3% bovine serum albumin and 0.1% saponin for 1 hour, followed by APC-conjugated goat anti-rabbit immunoglobulin G F(ab′)2 (no. 111-136-144, Jackson ImmunoResearch Laboratories) diluted at 1:1000 for 45 minutes.

    Techniques: Activity Assay, Isolation