gst ndr2 (Carna Inc)
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Carna Inc
gst ndr2
![NDR depletion abolishes mobility and metastasis properties in HBEC with RASSF1A depletion. HBEC-3 were transfected with non-silencing siRNA (siNeg), siRASSF1A and/or with siNDR1 or siNDR2. Experiences were performed 48 h after transfection. A549 cells were transfected with shcontrol, shNDR1 or shNDR2. a Wound healing assay of HBEC-3 cells on collagen IV coating were performed 48 h after transfection. Scale bar represents 100 μm. b Invasion capacity of HBEC-3 cells on BioCoat Matrigel Invasion Chamber. Relative invasion normalized to that of the cells transfected with siNeg. Scale bar represents 80 μm. c - d ShNDR1 or shNDR2-infected A549 cells suspension were injected subcutaneously in SCID −/− Beige mice. c Xenograft tumor size [length (L)/width (l)/thickness (e)]. NDR1 and <t>NDR2</t> mRNA levels of the injected cells, representative xenograft and NDR1 or NDR2 expression assayed by immunohistochemistry on xenografts are presented respectively on the left of, on the right of and below the xenografts growth curves. d Quantification of lung and liver microscopic nodules metastases for A549 cells expressing shNDR1, shNDR2 or shcontrol. Excised mice lung and liver as histologic photographs of the lung and liver metastases after injection with shNDR1, shNDR2 or shcontrol are presented below the quantification. Error bars indicate the standard error of the mean (SEM) ( n ≥ 3). * P < 0.05, ** P < 0.01 and *** P < 0.001, using an ANOVA test followed by Dunnett test](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1807/pmc06461807/pmc06461807__13046_2019_1145_Fig1_HTML.jpg)
Gst Ndr2, supplied by Carna Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/01-126/NDR2/pmc06461807-54-17-18
Average 96 stars, based on 1 article reviews
Gst Ndr2, supplied by Carna Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/01-126/NDR2/pmc06461807-54-17-18
Average 96 stars, based on 1 article reviews
gst ndr2 - by Bioz Stars,
2026-09
96/100 stars
Images
1) Product Images from "NDR2 kinase contributes to cell invasion and cytokinesis defects induced by the inactivation of RASSF1A tumor-suppressor gene in lung cancer cells"
Article Title: NDR2 kinase contributes to cell invasion and cytokinesis defects induced by the inactivation of RASSF1A tumor-suppressor gene in lung cancer cells
Journal: Journal of Experimental & Clinical Cancer Research : CR
doi: 10.1186/s13046-019-1145-8
Figure Legend Snippet: NDR depletion abolishes mobility and metastasis properties in HBEC with RASSF1A depletion. HBEC-3 were transfected with non-silencing siRNA (siNeg), siRASSF1A and/or with siNDR1 or siNDR2. Experiences were performed 48 h after transfection. A549 cells were transfected with shcontrol, shNDR1 or shNDR2. a Wound healing assay of HBEC-3 cells on collagen IV coating were performed 48 h after transfection. Scale bar represents 100 μm. b Invasion capacity of HBEC-3 cells on BioCoat Matrigel Invasion Chamber. Relative invasion normalized to that of the cells transfected with siNeg. Scale bar represents 80 μm. c - d ShNDR1 or shNDR2-infected A549 cells suspension were injected subcutaneously in SCID −/− Beige mice. c Xenograft tumor size [length (L)/width (l)/thickness (e)]. NDR1 and NDR2 mRNA levels of the injected cells, representative xenograft and NDR1 or NDR2 expression assayed by immunohistochemistry on xenografts are presented respectively on the left of, on the right of and below the xenografts growth curves. d Quantification of lung and liver microscopic nodules metastases for A549 cells expressing shNDR1, shNDR2 or shcontrol. Excised mice lung and liver as histologic photographs of the lung and liver metastases after injection with shNDR1, shNDR2 or shcontrol are presented below the quantification. Error bars indicate the standard error of the mean (SEM) ( n ≥ 3). * P < 0.05, ** P < 0.01 and *** P < 0.001, using an ANOVA test followed by Dunnett test
Techniques Used: Transfection, Wound Healing Assay, Infection, Suspension, Injection, Expressing, Immunohistochemistry
Figure Legend Snippet: NDR depletion abolishes YAP activation induced by RASSF1A silencing in HBEC cells. a - b HBEC-3 cells were transfected with siNeg, siRASSF1A and/or with siNDR1 or siNDR2. c - d Xenograft obtained after subcutaneous injection of shcontrol, shNDR1 or NDR2 H1299 cells. Nuclear YAP quantification by immunofluorescence ( a ) or by immunohistochemistry ( c ). b , d Quantification of CTGF (Bi, Di) & ANKRD1 (Bii, Dii) mRNA using actin as an internal control in HBEC-3 cells. Error bars indicate the SEM ( n ≥ 3). * P < 0.05, ** P < 0.01 and *** P < 0.001, using an ANOVA test followed by Dunnett’s test
Techniques Used: Activation Assay, Transfection, Injection, Immunofluorescence, Immunohistochemistry
Figure Legend Snippet: NDR2 interacts and phosphorylates GEF-H1 in HBEC. HBEC-3 or H1299 cells were transfected with siNeg and/or siRASSF1A, siNDR1, siNDR2, siGEF-H1, pcDNA3-NDR1, pcDNA3-NDR2 or pcB6-GEF-H1. a GST-RBD pull-down assay in H1299 cells. b Ser885 phosphorylation from GEF-H1 assayed by western blot following λ-phosphatase pre-treatment of the total protein extract or not and normalized with total GEF-H1 expression in HBEC-3 cells. c GST-NDR1 or -NDR2 pull-down assay using siRNA & GEF-H1 plasmid as controls. NDR2 phosphorylation on Ser265-GEFGH1 link with phosphorylation on Ser-885-GEF-H1A was assayed. NDR2 activity on HBEC-3 cells extracts previously transfected with pcB6-GEF-H1 wild type, mutated on Ser265 (S265A), or on Ser885 (S885A) was assayed by quantifying ser885-GEF-H1 phosphorylation status by western blot following normalization by total GEF-H1 expression. Error bars indicate the SEM ( n ≥ 3). * P < 0.05, ** P < 0.01 and *** P < 0.001, using an ANOVA test followed by Dunnett’s test
Techniques Used: Transfection, Pull Down Assay, Western Blot, Expressing, Plasmid Preparation, Activity Assay
Figure Legend Snippet: NDR2 and GEF-H1 are co-stained in HBEC-3 cells during both interphase and cell division. HBEC-3 cells were transfected with siNeg or siRASSF1A. a GEF-H1 and NDR2 co-staining assayed by immunofluorescence in HBEC-3 cells during interphase. b Representative images are shown for NDR2 and GEF-H1 during mitosis. Localization was identified by immunofluorescence and confocal microscopy. Costaining was evaluated by ImageJ software. HBEC-3 cells were also stained with DAPI for DNA and PSer885GEF-H1 during mitosis. Error bars indicate the SEM ( n ≥ 3). * P < 0.05, ** P < 0.01 and *** P < 0.001, using an ANOVA test followed by Dunnett’s test
Techniques Used: Staining, Transfection, Immunofluorescence, Confocal Microscopy, Software
Figure Legend Snippet: A proposal model for RASSF1A regulation of the NDR2/GEFH-1/RhoB/YAP axis
Techniques Used:
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Incubation:Article Title: NDR2 kinase contributes to cell invasion and cytokinesis defects induced by the inactivation of RASSF1A tumor-suppressor gene in lung cancer cells Article Snippet: .. For GTP-pulldown assays, cell lysates were incubated with beads glutathione-S-transferase (GST)-Rhotekin Rho binding domain (RBD), GST-NDR1 or Binding Assay:Article Title: NDR2 kinase contributes to cell invasion and cytokinesis defects induced by the inactivation of RASSF1A tumor-suppressor gene in lung cancer cells Article Snippet: .. For GTP-pulldown assays, cell lysates were incubated with beads glutathione-S-transferase (GST)-Rhotekin Rho binding domain (RBD), GST-NDR1 or Western Blot:Article Title: NDR2 kinase contributes to cell invasion and cytokinesis defects induced by the inactivation of RASSF1A tumor-suppressor gene in lung cancer cells Article Snippet: .. For GTP-pulldown assays, cell lysates were incubated with beads glutathione-S-transferase (GST)-Rhotekin Rho binding domain (RBD), GST-NDR1 or |
