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ires2 zsgreen  (TaKaRa)


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    Structured Review

    TaKaRa ires2 zsgreen
    Ires2 Zsgreen, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/0057vct/RLP-CRE-ZsGreen1-11/pmc08683524-55-18-19
    Average 91 stars, based on 1 article reviews
    ires2 zsgreen - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Generated:

    Article Title: Triciribine increases LDLR expression and LDL uptake through stabilization of LDLR mRNA
    Article Snippet: The amount of PCSK9 secreted by cells into culture medium was measured using Circulex Human PCSK9 ELISA Kit (MBL International, Woburn, MA) according to the manufacturer’s instructions. .. pLR1563-luc was kindly provided by Dr. Youngmi Kim Pak (Asian Institute for Life Sciences, University of Ulsan College of Medicine, Seoul, Korea) . pLR1563/mutSRE-1-luc was generated by mutating CC (underlined) in the LDLR promoter SRE-1 motif (ATCAC CC CAC) in pLR1563-luc to GG using QuickChange II XL mutagensis kit (Agilent Technologies). pmR-ZsGreen1-LDLR-3′UTR-wt was constructed by inserting a PCR fragment containing the 2.5 kbp of human LDLR 3′UTR downstream of ZsGreen1 gene in pmR-ZsGreen1 reporter plasmid (Clonetech). ..

    Article Title: Triciribine increases LDLR expression and LDL uptake through stabilization of LDLR mRNA.
    Article Snippet: The amount of PCSK9 secreted by cells into culture medium was measured using Circulex Human PCSK9 ELISA Kit (MBL International, Woburn, MA) according to the manufacturer’s instructions. .. DNA constructs, transfection and luciferase reporter assays. pLR1563-luc was kindly provided by Dr. Youngmi Kim Pak (Asian Institute for Life Sciences, University of Ulsan College of Medicine, Seoul, Korea)29. pLR1563/mutSRE-1-luc was generated by mutating CC (underlined) in the LDLR promoter SRE-1 motif (ATCACCCCAC) in pLR1563-luc to GG using QuickChange II XL mutagensis kit (Agilent Technologies). pmR-ZsGreen1-LDLR-3′UTR-wt was constructed by inserting a PCR fragment containing the 2.5 kbp of human LDLR 3′UTR downstream of ZsGreen1 gene in pmR-ZsGreen1 reporter plasmid (Clonetech). ..

    Construct:

    Article Title: Triciribine increases LDLR expression and LDL uptake through stabilization of LDLR mRNA
    Article Snippet: The amount of PCSK9 secreted by cells into culture medium was measured using Circulex Human PCSK9 ELISA Kit (MBL International, Woburn, MA) according to the manufacturer’s instructions. .. pLR1563-luc was kindly provided by Dr. Youngmi Kim Pak (Asian Institute for Life Sciences, University of Ulsan College of Medicine, Seoul, Korea) . pLR1563/mutSRE-1-luc was generated by mutating CC (underlined) in the LDLR promoter SRE-1 motif (ATCAC CC CAC) in pLR1563-luc to GG using QuickChange II XL mutagensis kit (Agilent Technologies). pmR-ZsGreen1-LDLR-3′UTR-wt was constructed by inserting a PCR fragment containing the 2.5 kbp of human LDLR 3′UTR downstream of ZsGreen1 gene in pmR-ZsGreen1 reporter plasmid (Clonetech). ..

    Article Title: Triciribine increases LDLR expression and LDL uptake through stabilization of LDLR mRNA.
    Article Snippet: The amount of PCSK9 secreted by cells into culture medium was measured using Circulex Human PCSK9 ELISA Kit (MBL International, Woburn, MA) according to the manufacturer’s instructions. .. DNA constructs, transfection and luciferase reporter assays. pLR1563-luc was kindly provided by Dr. Youngmi Kim Pak (Asian Institute for Life Sciences, University of Ulsan College of Medicine, Seoul, Korea)29. pLR1563/mutSRE-1-luc was generated by mutating CC (underlined) in the LDLR promoter SRE-1 motif (ATCACCCCAC) in pLR1563-luc to GG using QuickChange II XL mutagensis kit (Agilent Technologies). pmR-ZsGreen1-LDLR-3′UTR-wt was constructed by inserting a PCR fragment containing the 2.5 kbp of human LDLR 3′UTR downstream of ZsGreen1 gene in pmR-ZsGreen1 reporter plasmid (Clonetech). ..

    Polymerase Chain Reaction:

    Article Title: Triciribine increases LDLR expression and LDL uptake through stabilization of LDLR mRNA
    Article Snippet: The amount of PCSK9 secreted by cells into culture medium was measured using Circulex Human PCSK9 ELISA Kit (MBL International, Woburn, MA) according to the manufacturer’s instructions. .. pLR1563-luc was kindly provided by Dr. Youngmi Kim Pak (Asian Institute for Life Sciences, University of Ulsan College of Medicine, Seoul, Korea) . pLR1563/mutSRE-1-luc was generated by mutating CC (underlined) in the LDLR promoter SRE-1 motif (ATCAC CC CAC) in pLR1563-luc to GG using QuickChange II XL mutagensis kit (Agilent Technologies). pmR-ZsGreen1-LDLR-3′UTR-wt was constructed by inserting a PCR fragment containing the 2.5 kbp of human LDLR 3′UTR downstream of ZsGreen1 gene in pmR-ZsGreen1 reporter plasmid (Clonetech). ..

    Article Title: Triciribine increases LDLR expression and LDL uptake through stabilization of LDLR mRNA.
    Article Snippet: The amount of PCSK9 secreted by cells into culture medium was measured using Circulex Human PCSK9 ELISA Kit (MBL International, Woburn, MA) according to the manufacturer’s instructions. .. DNA constructs, transfection and luciferase reporter assays. pLR1563-luc was kindly provided by Dr. Youngmi Kim Pak (Asian Institute for Life Sciences, University of Ulsan College of Medicine, Seoul, Korea)29. pLR1563/mutSRE-1-luc was generated by mutating CC (underlined) in the LDLR promoter SRE-1 motif (ATCACCCCAC) in pLR1563-luc to GG using QuickChange II XL mutagensis kit (Agilent Technologies). pmR-ZsGreen1-LDLR-3′UTR-wt was constructed by inserting a PCR fragment containing the 2.5 kbp of human LDLR 3′UTR downstream of ZsGreen1 gene in pmR-ZsGreen1 reporter plasmid (Clonetech). ..

    Plasmid Preparation:

    Article Title: Triciribine increases LDLR expression and LDL uptake through stabilization of LDLR mRNA
    Article Snippet: The amount of PCSK9 secreted by cells into culture medium was measured using Circulex Human PCSK9 ELISA Kit (MBL International, Woburn, MA) according to the manufacturer’s instructions. .. pLR1563-luc was kindly provided by Dr. Youngmi Kim Pak (Asian Institute for Life Sciences, University of Ulsan College of Medicine, Seoul, Korea) . pLR1563/mutSRE-1-luc was generated by mutating CC (underlined) in the LDLR promoter SRE-1 motif (ATCAC CC CAC) in pLR1563-luc to GG using QuickChange II XL mutagensis kit (Agilent Technologies). pmR-ZsGreen1-LDLR-3′UTR-wt was constructed by inserting a PCR fragment containing the 2.5 kbp of human LDLR 3′UTR downstream of ZsGreen1 gene in pmR-ZsGreen1 reporter plasmid (Clonetech). ..

    Article Title: Protective role of cytoplasmic p21Cip1/Waf1 in apoptosis of CDK4/6 inhibitor‐induced senescence in breast cancer cells
    Article Snippet: Protein bands were visualized using an Amersham ImageQuantTM 800 Biomolecular Imager (General Electric Company). .. MDA‐MB‐231 cells were transfected with pEB Multi‐Neo vector (Fujifilm Wako Pure Chemical), in which genes encoding p21 with IRES2‐ZsGreen (Takara Bio) were inserted, using Lipofectamine 3000 (Invitrogen) and cultured in the presence of G418. ..

    Article Title: Triciribine increases LDLR expression and LDL uptake through stabilization of LDLR mRNA.
    Article Snippet: The amount of PCSK9 secreted by cells into culture medium was measured using Circulex Human PCSK9 ELISA Kit (MBL International, Woburn, MA) according to the manufacturer’s instructions. .. DNA constructs, transfection and luciferase reporter assays. pLR1563-luc was kindly provided by Dr. Youngmi Kim Pak (Asian Institute for Life Sciences, University of Ulsan College of Medicine, Seoul, Korea)29. pLR1563/mutSRE-1-luc was generated by mutating CC (underlined) in the LDLR promoter SRE-1 motif (ATCACCCCAC) in pLR1563-luc to GG using QuickChange II XL mutagensis kit (Agilent Technologies). pmR-ZsGreen1-LDLR-3′UTR-wt was constructed by inserting a PCR fragment containing the 2.5 kbp of human LDLR 3′UTR downstream of ZsGreen1 gene in pmR-ZsGreen1 reporter plasmid (Clonetech). ..

    Multiple Displacement Amplification:

    Article Title: Protective role of cytoplasmic p21Cip1/Waf1 in apoptosis of CDK4/6 inhibitor‐induced senescence in breast cancer cells
    Article Snippet: Protein bands were visualized using an Amersham ImageQuantTM 800 Biomolecular Imager (General Electric Company). .. MDA‐MB‐231 cells were transfected with pEB Multi‐Neo vector (Fujifilm Wako Pure Chemical), in which genes encoding p21 with IRES2‐ZsGreen (Takara Bio) were inserted, using Lipofectamine 3000 (Invitrogen) and cultured in the presence of G418. ..

    Transfection:

    Article Title: Protective role of cytoplasmic p21Cip1/Waf1 in apoptosis of CDK4/6 inhibitor‐induced senescence in breast cancer cells
    Article Snippet: Protein bands were visualized using an Amersham ImageQuantTM 800 Biomolecular Imager (General Electric Company). .. MDA‐MB‐231 cells were transfected with pEB Multi‐Neo vector (Fujifilm Wako Pure Chemical), in which genes encoding p21 with IRES2‐ZsGreen (Takara Bio) were inserted, using Lipofectamine 3000 (Invitrogen) and cultured in the presence of G418. ..

    Article Title: Triciribine increases LDLR expression and LDL uptake through stabilization of LDLR mRNA.
    Article Snippet: The amount of PCSK9 secreted by cells into culture medium was measured using Circulex Human PCSK9 ELISA Kit (MBL International, Woburn, MA) according to the manufacturer’s instructions. .. DNA constructs, transfection and luciferase reporter assays. pLR1563-luc was kindly provided by Dr. Youngmi Kim Pak (Asian Institute for Life Sciences, University of Ulsan College of Medicine, Seoul, Korea)29. pLR1563/mutSRE-1-luc was generated by mutating CC (underlined) in the LDLR promoter SRE-1 motif (ATCACCCCAC) in pLR1563-luc to GG using QuickChange II XL mutagensis kit (Agilent Technologies). pmR-ZsGreen1-LDLR-3′UTR-wt was constructed by inserting a PCR fragment containing the 2.5 kbp of human LDLR 3′UTR downstream of ZsGreen1 gene in pmR-ZsGreen1 reporter plasmid (Clonetech). ..

    Cell Culture:

    Article Title: Protective role of cytoplasmic p21Cip1/Waf1 in apoptosis of CDK4/6 inhibitor‐induced senescence in breast cancer cells
    Article Snippet: Protein bands were visualized using an Amersham ImageQuantTM 800 Biomolecular Imager (General Electric Company). .. MDA‐MB‐231 cells were transfected with pEB Multi‐Neo vector (Fujifilm Wako Pure Chemical), in which genes encoding p21 with IRES2‐ZsGreen (Takara Bio) were inserted, using Lipofectamine 3000 (Invitrogen) and cultured in the presence of G418. ..

    Luciferase:

    Article Title: Triciribine increases LDLR expression and LDL uptake through stabilization of LDLR mRNA.
    Article Snippet: The amount of PCSK9 secreted by cells into culture medium was measured using Circulex Human PCSK9 ELISA Kit (MBL International, Woburn, MA) according to the manufacturer’s instructions. .. DNA constructs, transfection and luciferase reporter assays. pLR1563-luc was kindly provided by Dr. Youngmi Kim Pak (Asian Institute for Life Sciences, University of Ulsan College of Medicine, Seoul, Korea)29. pLR1563/mutSRE-1-luc was generated by mutating CC (underlined) in the LDLR promoter SRE-1 motif (ATCACCCCAC) in pLR1563-luc to GG using QuickChange II XL mutagensis kit (Agilent Technologies). pmR-ZsGreen1-LDLR-3′UTR-wt was constructed by inserting a PCR fragment containing the 2.5 kbp of human LDLR 3′UTR downstream of ZsGreen1 gene in pmR-ZsGreen1 reporter plasmid (Clonetech). ..



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