guanidinium thiocyanate (Chem Impex International)
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guanidinium thiocyanate
Guanidinium Thiocyanate, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/00522/Guanidine+thiocyanate/pm37735163-304-34-37
Average 95 stars, based on 3 article reviews
Guanidinium Thiocyanate, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/00522/Guanidine+thiocyanate/pm37735163-304-34-37
Average 95 stars, based on 3 article reviews
guanidinium thiocyanate - by Bioz Stars,
2026-09
95/100 stars
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other:Article Title: Rapid and Efficient Isolation of Total RNA-Bound Proteomes by Liquid Emulsion–Assisted Purification of RNA-Bound Protein (LEAP-RBP) Article Snippet: Incubation:Article Title: Adaptation of the protein misfolding cyclic amplification (PMCA) technique for the screening of anti-prion compounds Article Snippet: Five μL of PK-digested PMCA-products were directly applied onto a nitrocellulose membrane (GE Healthcare Amersham, Chicago, IL, USA) using a Bio-Dot Apparatus (Bio-Rad Laboratories, Hercules, CA, USA). .. The membranes were dried using a blow dryer and then transferred to an incubation chamber containing 10 mL of 3 M Article Title: Adaptation of the protein misfolding cyclic amplification (PMCA) technique for the screening of anti‐prion compounds Article Snippet: © 2024 Federation of American Societies for Experimental Biology.. Abbreviations: 96wpPMCA, 96 well plate – protein misfolding cyclic amplification; CWD, chronic wasting disease; DMSO, dimethyl sulfoxide; EDTA, ethylenediaminetetraacetic acid; EtOH, ethanol; PBS, phosphate buffer saline; PK, proteinase K; PMCA, protein misfolding cyclic amplification; PMSF, phenylmethane sulfonyl fluoride; RML, Rocky Mountain Laboratories; SSLOW, synthetic strain leading to overweight; TSE, transmissible spongiform e nce pha lop ath y.. 1Department of Neurology, The University of Texas Health Science Center at Houston, Houston, Texas, USA 2Centro Integrativo de Biologia y Quimica Aplicada (CIBQA), Universidad Bernardo O'Higgins, Santiago, Chile Blocking Assay:Article Title: Adaptation of the protein misfolding cyclic amplification (PMCA) technique for the screening of anti-prion compounds Article Snippet: Five μL of PK-digested PMCA-products were directly applied onto a nitrocellulose membrane (GE Healthcare Amersham, Chicago, IL, USA) using a Bio-Dot Apparatus (Bio-Rad Laboratories, Hercules, CA, USA). .. The membranes were dried using a blow dryer and then transferred to an incubation chamber containing 10 mL of 3 M Article Title: Adaptation of the protein misfolding cyclic amplification (PMCA) technique for the screening of anti‐prion compounds Article Snippet: © 2024 Federation of American Societies for Experimental Biology.. Abbreviations: 96wpPMCA, 96 well plate – protein misfolding cyclic amplification; CWD, chronic wasting disease; DMSO, dimethyl sulfoxide; EDTA, ethylenediaminetetraacetic acid; EtOH, ethanol; PBS, phosphate buffer saline; PK, proteinase K; PMCA, protein misfolding cyclic amplification; PMSF, phenylmethane sulfonyl fluoride; RML, Rocky Mountain Laboratories; SSLOW, synthetic strain leading to overweight; TSE, transmissible spongiform e nce pha lop ath y.. 1Department of Neurology, The University of Texas Health Science Center at Houston, Houston, Texas, USA 2Centro Integrativo de Biologia y Quimica Aplicada (CIBQA), Universidad Bernardo O'Higgins, Santiago, Chile |
![SCI leads to increased α2δ1 and RAMP1 expression in L3–5 DRG neurons and eWAT, respectively (A) Scatterplot shows α2δ1 expression in Fluoro-Gold retrogradely labeled L3–5 DRG neurons at 7DPO (sham n = 4 and SCI n = 5 biological replicates, 419–486 neurons/experimental condition). Inset: α2δ1 expression in small-diameter (<20 μm) DRG neurons. Mean and SEM (mixed model with a random subject intercept to account for within-subject correlation ∗∗∗ p < 0.001). (B) Distribution of α2δ1 expression in retrogradely labeled CGRP-positive L3–5 DRG neurons at 7DPO. These neurons are a subset of (A) (two-sample Kolmogorov-Smirnov test ∗∗∗ p < 0.001; sham n = 4 and SCI n = 5 biological replicates; 161–233 neurons/experimental condition). (C) Representative fluorescence images of L3–5 DRG at 7DPO. Arrowheads indicate Fluoro-Gold retrogradely labeled CGRP-positive DRG neurons whose centrally projecting axon was severed after SCI. Scale, 50 μm. (D) Distribution of Cav2.1 expression in retrogradely labeled CGRP-positive L3–5 DRG neurons at 7DPO (two-sample Kolmogorov-Smirnov test ∗∗∗ p < 0.001; sham n = 4 and SCI n = 5 biological replicates; 109–236 neurons/experimental condition). (E) CGRP concentration in eWAT at 7DPO. Mean and SEM (unpaired two-tailed Student’s t test ∗ p < 0.05, sham n = 4 and SCI n = 4 biological replicates). (F) Representative fluorescence images of eWAT at 7DPO. Scale, 100 μm. (G) Quantification of (F). Mean and SEM (unpaired two-tailed Student’s t test ∗∗ p < 0.01, sham n = 4 and SCI n = 3 biological replicates). (H) Representative fluorescence images and 3D reconstruction of nociceptive nerve terminals in the unsectioned eWAT at 7DPO. Scale, 500 μm. (I) Quantification of (H). Mean and SEM (unpaired two-tailed Student’s t test, ns [not significant], sham n = 3 and SCI n = 3 biological replicates). (J) ex vivo eWAT lipolysis under basal (vehicle) and CGRP-stimulated (1 μM) conditions. Scatterplot shows fold change in glycerol release (paired two-tailed Student’s t test ∗∗ p < 0.01, n = 4 biological replicates). (K) Representative fluorescence and DIC images of cultured adult eWAT adipocytes loaded with the calcium indicator Fluo-4 AM. Scale, 100 μm. (L) Calcium response in primary eWAT adipocyte after vehicle (dH 2 O) and CGRP (43 μM) administration (vehicle n = 81 and CGRP n = 100 eWAT adipocyte traces from 5 independent experiments/experimental condition). (M) Quantification of (L). Mean and SEM (unpaired two-tailed Student’s t test ∗∗∗ p < 0.001, vehicle n = 81 and CGRP n = 100 eWAT adipocyte traces from 5 independent experiments/experimental condition).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8638/pmc11148638/pmc11148638__gr3.jpg)
![Cacna2d1 conditional deletion in DRG neurons normalizes eWAT lipolysis after SCI (A) Experimental scheme and timeline of AAV injection to transduce L3–5 DRG. (B) Real-time qPCR shows Cacna2d1 normalized expression in adult L3–5 DRG from Cacna2d1 fl/fl mice injected with either AAV-GFP or AAV-Cre. Mean and SEM (unpaired two-tailed Student’s t test ∗ p < 0.05, AAV-Ctr n = 4 and AAV-Cre n = 4 biological replicates). (C) Representative hematoxylin and eosin images of eWAT at 7 days post injury (DPI). Scale, 100 μm. (D) Quantification of adipocyte area in (C). Mean and SEM (two-way ANOVA ∗∗ p < 0.01 and ∗∗∗ p < 0.001, ns [not significant], AAV-GFP/SCI n = 3 and AAV-Cre/SCI n = 3 biological replicates; 2,063–2,322 adipocytes/experimental condition). (E) Ex vivo eWAT lipolysis at 7DPI. Mean and SEM (Kruskal-Wallis test followed by Dunn’s multiple comparisons test ∗∗ p < 0.01, ns [not significant], AAV-GFP n = 6, AAV-GFP/SCI n = 5, and AAV-Cre/SCI n = 6 biological replicates). The AAV-GFP samples are the same as in <xref ref-type=](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8638/pmc11148638/pmc11148638__gr4.jpg)
Figure S4 E. (F) Immunoblots show pHSL, HSL, and ATGL expression in eWAT at 7DPI. GAPDH is used as a loading control. (G) Quantification of (F). Mean and SEM (unpaired two-tailed Student’s t test ∗∗ p < 0.01, AAV-GFP/SCI n = 4 and AAV-Cre/SCI n = 4 biological replicates). (H) CGRP concentration in eWAT at 7DPI. Mean and SEM (unpaired two-tailed Student’s t test ∗∗ p < 0.01, AAV-GFP/SCI n = 4 and AAV-Cre/SCI n = 4 biological replicates). (I) Representative hematoxylin and eosin images of eWAT at 7DPI. Scale, 100 μm. (J) Quantification of adipocyte area in (I). Mean and SEM (two-way ANOVA ∗∗∗ p < 0.001, ns [not significant], Na v 1.8-Cre n = 5 and Na v 1.8-Cre/ Cacna2d1 fl/fl n = 5 biological replicates; 2,732–3,170 adipocytes/experimental condition). (K) Ex vivo eWAT lipolysis at 7DPI. Mean and SEM (unpaired two-tailed Student’s t test ∗ p < 0.05, Na v 1.8-Cre n = 4 and Na v 1.8-Cre/ Cacna2d1 fl/fl n = 5 biological replicates). (L) Immunoblots show pHSL, HSL, and ATGL expression in eWAT at 7DPI. GAPDH is used as a loading control. (M) Quantification of (L). Mean and SEM (unpaired two-tailed Student’s t test ∗ p < 0.05, Na v 1.8-Cre n = 4 and Na v 1.8-Cre/ Cacna2d1 fl/fl n = 5 biological replicates). (N) CGRP concentration in eWAT at 7DPI. Mean and SEM (unpaired two-tailed Student’s t test ∗∗ p < 0.01, Na v 1.8-Cre n = 8 and Na v 1.8-Cre/ Cacna2d1 fl/fl n = 10 biological replicates). " width="100%" height="100%">
![α2δ1 pharmacological blockade via GBP administration normalizes eWAT lipolysis and reduces ectopic lipid accumulation in the liver after SCI (A) Experimental scheme. (B) Mouse body weight. Mean and SEM (mixed model with a random subject intercept to account for within-subject correlation ∗ p < 0.05 and ∗∗∗ p < 0.001, ns [not significant], sham n = 10, vehicle/SCI n = 11, and GBP/SCI n = 11 biological replicates). (C) Representative images of hematoxylin and eosin-stained eWAT sections 28DPO. Scale, 100 μm. (D) Quantification of adipocyte area in (C). Mean and SEM (two-way ANOVA ∗ p < 0.05 and ∗∗∗ p < 0.001, ns [not significant], vehicle/SCI n = 5 and GBP/SCI n = 4 biological replicates; 654–1,051 adipocytes/experimental condition). (E) Ex vivo eWAT lipolysis at 30DPI (unpaired two-tailed Student’s t test ∗∗∗ p < 0.001, vehicle/SCI n = 6 and GBP/SCI n = 6 biological replicates). (F) Glycerol concentration in the serum at 30DPI. Mean and SEM (unpaired two-tailed Student’s t test ∗ p < 0.05, vehicle/SCI n = 5 and GBP/SCI n = 4 biological replicates). (G) Immunoblots show pHSL, HSL, and ATGL expression in eWAT at 30DPI. GAPDH is used as a loading control. (H) Quantification of (G). Mean and SEM (unpaired two-tailed Student’s t test ∗ p < 0.05, ns [not significant], vehicle/SCI n = 3 and GBP/SCI n = 3 biological replicates). (I) CGRP concentration in the serum at 7DPI. Mean and SEM (unpaired 2-tailed Student’s t test ∗ p < 0.05, vehicle/SCI n = 6 and GBP/SCI n = 5 biological replicates). (J) Representative images of liver sections stained with oil red O to visualize lipid accumulation. Scale, 50 μm. (K) Quantification of (J). Mean and SEM (two-way ANOVA ∗ p < 0.05 and ∗∗∗ p < 0.001, ns [not significant], vehicle/SCI n = 4 and GBP/SCI n = 5 biological replicates). (L) Metabolic cage assessment (CLAMS) of oxygen consumption (VO 2 ) and respiratory exchange rate (RER) at 1 month after SCI. Mean and SEM (mixed model with a random subject intercept to account for within-subject correlation, p values for group/period interaction are provided, sham n = 4, SCI n = 4, vehicle/SCI (middle) n = 4, GBP/SCI n = 5, vehicle/SCI (right) n = 4, and dGBP/SCI n = 5).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8638/pmc11148638/pmc11148638__gr5.jpg)
