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nigericin cayman  (Chem Impex International)


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    Structured Review

    Chem Impex International nigericin cayman
    Nigericin Cayman, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/00359/Nigericin+sodium+salt/pmc07971856-313-2-8
    Average 95 stars, based on 3 article reviews
    nigericin cayman - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Solvent:

    Article Title: Human NACHT, LRR, and PYD domain-containing protein 3 (NLRP3) inflammasome activity is regulated by and potentially targetable through Bruton tyrosine kinase.
    Article Snippet: 4x10 4 Human embryonic kidney (HEK) 293T 209 cells were plated, transiently transfected, fixed with 100% methanol, stained for nucleic acids (To-210 pro-3, Thermo Fisher) and BTK-HA (anti-HA-Alexa 594). .. Immortalized Nlrp3 KO macrophages 211 overexpressing NLRP3-FLAG and ASC-mCerulean were pretreated with ibrutinib or CGI1745 (and a 212 solvent control) for 10 min or 60 min, respectively, before stimulation with either 5 μM Nigericin (Life 213 Technologies) for 60 min or 1 mM Leu-Leu-OMe HCl (Chem-Impex) for 90 min, then fixed (4% 214 formaldehyde), and nucleic acids stained (DRAQ5, eBioscience). .. Details regarding analysis using a 215 Zeiss confocal microscope and image quantification using ImageJ or CellProfiler are described in 216 Supplemental Information.

    Control:

    Article Title: Human NACHT, LRR, and PYD domain-containing protein 3 (NLRP3) inflammasome activity is regulated by and potentially targetable through Bruton tyrosine kinase.
    Article Snippet: 4x10 4 Human embryonic kidney (HEK) 293T 209 cells were plated, transiently transfected, fixed with 100% methanol, stained for nucleic acids (To-210 pro-3, Thermo Fisher) and BTK-HA (anti-HA-Alexa 594). .. Immortalized Nlrp3 KO macrophages 211 overexpressing NLRP3-FLAG and ASC-mCerulean were pretreated with ibrutinib or CGI1745 (and a 212 solvent control) for 10 min or 60 min, respectively, before stimulation with either 5 μM Nigericin (Life 213 Technologies) for 60 min or 1 mM Leu-Leu-OMe HCl (Chem-Impex) for 90 min, then fixed (4% 214 formaldehyde), and nucleic acids stained (DRAQ5, eBioscience). .. Details regarding analysis using a 215 Zeiss confocal microscope and image quantification using ImageJ or CellProfiler are described in 216 Supplemental Information.

    Staining:

    Article Title: Human NACHT, LRR, and PYD domain-containing protein 3 (NLRP3) inflammasome activity is regulated by and potentially targetable through Bruton tyrosine kinase.
    Article Snippet: 4x10 4 Human embryonic kidney (HEK) 293T 209 cells were plated, transiently transfected, fixed with 100% methanol, stained for nucleic acids (To-210 pro-3, Thermo Fisher) and BTK-HA (anti-HA-Alexa 594). .. Immortalized Nlrp3 KO macrophages 211 overexpressing NLRP3-FLAG and ASC-mCerulean were pretreated with ibrutinib or CGI1745 (and a 212 solvent control) for 10 min or 60 min, respectively, before stimulation with either 5 μM Nigericin (Life 213 Technologies) for 60 min or 1 mM Leu-Leu-OMe HCl (Chem-Impex) for 90 min, then fixed (4% 214 formaldehyde), and nucleic acids stained (DRAQ5, eBioscience). .. Details regarding analysis using a 215 Zeiss confocal microscope and image quantification using ImageJ or CellProfiler are described in 216 Supplemental Information.



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