gtp  (Chem Impex International)

Journal: eLife

Article Title: A robust method for particulate detection of a genetic tag for 3D electron microscopy

doi: 10.7554/eLife.64630

Figure Lengend Snippet:

Article Snippet: Chemical compound, drug , GTP , Chem-Impex , 00348 , .

Techniques: Transfection, Construct, Recombinant, Sequencing, In Vitro, Labeling, Electron Microscopy, Inhibition, Purification, Software

Journal: Cell communication and signaling : CCS

Article Title: PRMT5 enhances tumorigenicity and glycolysis in pancreatic cancer via the FBW7/cMyc axis.

doi: 10.1186/s12964-019-0344-4

Figure Lengend Snippet: Fig. 5 PRMT5-mediated epigenetic silencing of FBW7 leads to increased cMyc levels a. In PRMT5-silenced MIA PaCa-2 and SW1990 cells, the mRNA levels of FBW7 were increased. b. PRMT5 knockdown increased FBW7 protein levels. c. PRMT5 expression was negatively correlated with FBW7 expression in the TCGA-PAAD dataset of pancreatic cancer patients. d. In HPDE cells, overexpressing PRMT5 decreased FBW7 mRNA levels, but PRMT5DN did not regulate FBW7 expression. e. Overexpressing PRMT5 in HPDE cells decreased FBW7 protein levels, but PRMT5DN exerted no impact on FBW7 protein levels. f. The results of the dual luciferase assay in HEK-293 T cells showed that although PRMT5 suppressed FBW7 promoter activity, PRMT5DN did not significantly regulate FBW7 promoter activity. g-h. The ChIP assay results demonstrated that PRMT5 occupied the promoter region enriched with CpG islands. i. PRMT5 knockdown decreased the occupancy of the heterochromatin markers H4R3me2 and H3K9me3, and the euchromatin marker H3K9ac, which reflects active transcription, was increased in MIA PaCa-2 and SW1990 cells with PRMT5 knockdown. j. Finally, we performed ChIP in HPDE cells with low PRMT5 expression. The ChIP results showed that PRMT5 increased the occupancy of heterochromatin markers such as H4MR3me2 and H3K9me3 and decreased that of the active chromatin marker H3K9ac decreased. However, the transferase-dead PRMT5DN mutant had no such effect

Article Snippet: The anti-FBW7 IHC antibody for the detection of FBW7 was obtained from Bethyl Laboratories.

Techniques: Knockdown, Expressing, Luciferase, Activity Assay, Marker, Mutagenesis

Journal: Cell communication and signaling : CCS

Article Title: PRMT5 enhances tumorigenicity and glycolysis in pancreatic cancer via the FBW7/cMyc axis.

doi: 10.1186/s12964-019-0344-4

Figure Lengend Snippet: Fig. 6 PRMT5 regulates proliferation and aerobic glycolysis via the FBW7/cMyc axis a. Overexpression of wild-type FBW7 in PRMT5-overexpressing HPDE cells attenuated the increase in the cMyc protein level, but the FBW7R465H mutant had no such effect. b. The CCK-8 assay results demonstrated that wild-type FBW7 decreased the increase in cell viability caused by PRMT5, while the FBW7R465H mutant, which lacked enzymatic activity, did not. c. FBW7 suppressed the increase in glucose uptake caused by PRMT5 in HPDE cells, while the FBW7R465H mutant did not. d. FBW7 inhibited the increase in lactate production induced by PRMT5 in HPDE cells, but the FBW7R465H mutant had little impact. e-f. The ECAR measurement results showed that FBW7 mitigated the increase in glycolysis and glycolytic capacity caused by PRMT5, but the FBW7R465H

Article Snippet: The anti-FBW7 IHC antibody for the detection of FBW7 was obtained from Bethyl Laboratories.

Techniques: Over Expression, Mutagenesis, CCK-8 Assay, Activity Assay

Journal: Cell communication and signaling : CCS

Article Title: PRMT5 enhances tumorigenicity and glycolysis in pancreatic cancer via the FBW7/cMyc axis.

doi: 10.1186/s12964-019-0344-4

Figure Lengend Snippet: Fig. 7 Schematic representation of the working model In pancreatic cancer, upregulated PRMT5 could epigenetically silence the expression of the E3 ubiquitin ligase FBW7, resulting in cMyc stabilization and a subsequent increase in the proliferation of and glycolysis in pancreatic cancer cells

Article Snippet: The anti-FBW7 IHC antibody for the detection of FBW7 was obtained from Bethyl Laboratories.

Techniques: Expressing, Ubiquitin Proteomics

Journal: Biochemical Engineering Journal

Article Title: Modelling of amorphous cellulose depolymerisation by cellulases, parametric studies and optimisation

doi: 10.1016/j.bej.2015.10.017

Figure Lengend Snippet: Representation of uncertainty of the model predications for cellulose, glucose, cellobiose, and cellotriose over hydrolysis with all the parameters (dim θ = 27). Quasi Monte Carle simulations (1000 samples, white blue lines), mean (→), and 10th ( ) and 90th ( ) percentile of the predictions. Typical cases: (a), starting non-crystalline cellulose concentrations of 10, 25, 50, and 100 g/L, and 6 FPU/g-glucan Spezyme CP cellulases loading in all processes; (b), Spezyme CP cellulases loadings of 0.5, 1.5, 3, and 6 FPU/g-glucan, and 50 g/L starting non-crystalline cellulose concentration in all processes.

Article Snippet: One set of data for non-crystalline cellulose hydrolysis is used for parameter estimation , in which Spezyme CP (Genencor, lot no. 301-00348-257) had an average activity of 31.2 filter paper units (FPU)/mL and was diluted to 1 and 3 FPU by adding buffer solutions.

Techniques: Concentration Assay

Journal: Biochemical Engineering Journal

Article Title: Modelling of amorphous cellulose depolymerisation by cellulases, parametric studies and optimisation

doi: 10.1016/j.bej.2015.10.017

Figure Lengend Snippet: Representation of uncertainty of model predictions for cellulose, glucose, cellobiose, and cellotriose over hydrolysis with the reduced set of parameters (dim θ = 13). Quasi Monte Carle simulations (1000 samples, white blue lines), mean (→), and 10th ( ) and 90th ( ) percentile of the predictions. Two hydrolysis processes: (a), Spezyme CP cellulases loading = 1 FPU/g-glucan; (b), Spezyme CP cellulases loading = 3 FPU/g-glucan.

Article Snippet: One set of data for non-crystalline cellulose hydrolysis is used for parameter estimation , in which Spezyme CP (Genencor, lot no. 301-00348-257) had an average activity of 31.2 filter paper units (FPU)/mL and was diluted to 1 and 3 FPU by adding buffer solutions.

Techniques: